improvement of single domain antibody stability by disul ﬁ de bond introduction

Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulﬁde isomerase by disulﬁde bond formation with free cysteines in the substrate-binding domain pptx

Ngày tải lên : 14/03/2014, 23:20

... crosslink by the single- letter code The PDI peptide side of the disul de bond is named R1, and the bacitracin side R2 (C) MALDI-TOF MS spectrum of peptides generated by tryptic digestion of the PDI–bacitracin ... involvement of disul de bond formation We hypothesized that bacitracin may react with cysteines on PDI to form a disul de bond Using MALDI-TOF ⁄ TOF MS analysis, we were able to show that disul de bond ... Bacitracin forms a disul de bond with free PDI cysteines Fig Disul de bond formation between bacitracin and Cys345 on PDI (A) Scheme of thiol formation of the thiazoline ring of bacitracin A with...

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[Khiem] improvement of matrix converter drive reliability by online fault detection and a fault tolerant switching strategy IEEE trans

Ngày tải lên : 13/05/2014, 00:55

... B.S degree (with honors) in the ﬁeld of electrical engineering from Hanoi University of Science and Technology, Hanoi, in 2009 He is currently working toward the M.S degree in the Department of ... practice, the DSP determines whether the output phase current is inside a small dead band , because a practical system often contains noise in the current NGUYEN-DUY et al.: IMPROVEMENT OF MATRIX CONVERTER ... output Thus, at the end of the ﬁrst stage test, the number of possible answers has been reduced from nine to three In the second stage of fault detection, the exact location of the open switch is...

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Báo cáo hóa học: " A Temperature Window for the Synthesis of Single-Walled Carbon Nanotubes by Catalytic Chemical Vapor Deposition of CH4 over Mo2-Fe10/MgO Catalyst" pptx

Ngày tải lên : 22/06/2014, 00:20

... shown in the results and are coated by plenty of amorphous carbons Conclusions A temperature window of SWCNTs growth by catalytic chemical vapor deposition of CH4 over Mo-Fe/MgO catalyst has been ... (Fig 4) showing the dependence of ID/IG on the growth temperature From Fig 4, two kinds of ID/IG distributions can clearly be distinguished From 750 to 900 °C, the ID/IG decreases with increasing ... In Fig 2a, only the G band (tangential mode), D band (related to disordered graphite or amorphous) and a shoulder at 1604 cm-1 (the fundamental E2g mode of graphite) are presented In the lower...

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quality improvement of bixin from annatto seed by modified extraction methods

Ngày tải lên : 29/11/2014, 08:21

... acetone, and sodium hydroxide solution 23 70 Bixin degradation (%) 60 50 40 deg Extraction by soybean oil degraded bixin acetone Extraction by 30 Extraction degraded by NaOH 20 10 20 40 60 80 ... percentage of degraded yellow products (C17 species) up to 40% of commercial oil soluble annatto products Whereas, degraded amounts of bixin in both extraction processes by acetone and sodium hydroxide ... by aluminum foil) containing soybean oil at 100oC for 20 Determination of bixin degradation The degradation of bixin was investigated by measuring bixin content in the samples, which were added...

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Báo cáo Y học: Disul®de bond formation through Cys186 facilitates functionally relevant dimerization of trimeric hyaluronan-binding protein 1 (HABP1)/p32/gC1qR docx

Ngày tải lên : 17/03/2014, 17:20

... sensitivity of 0.3 lM using copper nitrate as standard solution HgCl2-induced disul de linkage of HABP1 HgCl2-induced disul de linkage of two cysteine residues was achieved by a previously described ... the sequence-derived molecular mass that may arise from modi®ed electrophoretic mobility as a result of neutralization of positive charges of the lysine side chain by glutaraldehyde Oligomeric ... spectro¯uorimeter and Professor P Balaram of IISc, Bangalore for useful discussions The Department of Science and Technology and the Department of Biotechnology, Government of India, New Delhi have ®nancially...

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Báo cáo khoa học: Human acid sphingomyelinase Assignment of the disulﬁde bond pattern ppt

Ngày tải lên : 31/03/2014, 07:20

... SAP-type activator domain [13] The disul de bond Fig Disul de bond pattern and domain structure of haSMase The identiﬁed disul de bond structure of haSMase is presented The N-terminus of native haSMase ... FEBS 2003 Disul de bonds of human acid sphingomyelinase (Eur J Biochem 270) 1083 Glycosylation analysis – identiﬁcation of a peptide with two disul de bonds RP-HPLC of the peptides of the tryptic ... and by the extreme hydrophobicity of peptide T5 + T13 As these disul de bonds are part of the highly conserved SAP -domain of haSMase [13], we assume that the arrangement of the disul de bonds...

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Báo cáo hóa học: " Research Article Inequalities for Single Crystal Tube Growth by Edge-Deﬁned Film-Fed Growth Technique" ppt

Ngày tải lên : 22/06/2014, 02:20

... ∗ and α r1 de nition of r ∗ Journal of Inequalities and Applications 15 Therefore, r ∗ de ned by 3.14 satisﬁes r ∗ < m · Rgi tan π/2 − αg we remark that from the de nition In order to show ... αg Therefore, r∗ , de ned by 2.14 , satisﬁes r∗ > Rge /n − tan π/2 − αg we remark that from the de nition In order to show that z r∗ 2.14 of r∗ it follows that at least one of the following three ... Proof It is similar to the proof of Theorem 2.9 De nition 3.9 A solution z z r of 3.1 which describes the inner free surface of a static meniscus is said to be stable if it is a weak minimum of...

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Tài liệu Báo cáo khoa học: Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulﬁde bond formation and their contribution to venom complexity pdf

Ngày tải lên : 18/02/2014, 18:20

... inspection of the disul de bond pairing in the disintegrin-like domain of VAP2b indicates that the disul de bond pattern determined for catrocollastatin C could not occur without signiﬁcant structural ... residues with three disul de bonds The disul de bond pattern of the PIII proteinase domains appears to be different from that observed in the PI or PII proteinase domains (Fig 4) Most of the PIIIs have ... This underscores the point that similarly folded domains can support different disul de bond patterns Second, and perhaps most interesting, is the observation that the disul de bond pattern of VAP2B...

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Báo cáo khoa học: Cytoglobin conformations and disulﬁde bond formation pdf

Ngày tải lên : 15/03/2014, 11:20

... could depend on the recent history of binding of an external ligand For both Cygb and Ngb [14], the disul de bond influences the final position of the E-helix, which, in turn, modifies the affinity ... (intramolecular disul de bond) form and a reduced (disul de free) Cygb form is evident in the kinetics; such a transition affects the E-helix position, allowing a fine modulation of the endogenous His E7 affinity ... basic properties of Cygb, namely the role of the disul de bond in protein function and its involvement in the tertiary and quaternary structures Cytoglobin conformations and disul de bond formation...

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Báo cáo khoa học: Protein folding and disulﬁde bond formation in the eukaryotic cell pptx

Ngày tải lên : 29/03/2014, 22:21

... formation of protein disul des is catalysed by thiol disul de oxidoreductases through thiol disul de exchange reactions These enzymes have the capacity to oxidize, reduce or isomerize disul de bonds, ... the requirement for disul de bonds, which form between the SH groups (thiols) of two cysteine residues in a relatively oxidizing environment The disul de bond offers structural stability, and may ... the low-density lipoprotein receptor (LDLR) and its domains [9] Calcium seems to compete with disul de bond formation in the LDLR, and in the presence of this cation, non-native disul de bonds...

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Báo cáo khoa học: Simultaneous improvement of catalytic activity and thermal stability of tyrosine phenol-lyase by directed evolution ppt

Ngày tải lên : 07/03/2014, 00:20

... mutation of Thr15 to Ala in the vicinity of the hydrophobic core induces a tighter binding of the cofactor in C freundii TPL, thereby reducing the decomposition rate of the cofactor by a Pictet–Spengler ... show the trajectory of stability and activity mutations, respectively The gray-shaded ellipse encircles S1–S3 and AS1–AS3 hits that only include stability mutations no signiﬁcant improvement in activity, ... gray-shaded ellipse in Fig 1) Meanwhile, the four other mutants (AS4–AS7) included mutations from both the stability and activity mutants, and demonstrated a signiﬁcant improvement in both stability...

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Báo cáo khoa học: A strategy for the generation of speciﬁc human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx

Ngày tải lên : 23/03/2014, 13:20

... in antibody variable chain family representation, each V family of variable regions (VH or VL), was amplified by independent PCR In a second PCR step, the sequence of the linker peptide was added ... II-9.2.2) was purified from venom obtained by electric stimulation of scorpions of the species Centruroides noxius Hoffmann The venom was purified by Sephadex G-50 gel filtration and cation-exchange chromatography ... purified by Ni2+-NTA affinity chromatography (Qiagen, Hilden, Germany), and eluted with mL of 250 mm imidazole Finally, scFv preparations were purified by gel filtration chromatography on a SuperdexTM...

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Investigation of SPR and electrochemical detection of antigen with polypyrrole functionalized by biotinylated single chain antibody a review

Ngày tải lên : 13/09/2015, 18:05

... good stability of the sensor (figure not shown) Reproducible responses are obtained between analyses This specificity of the antibody antigen complex is confirmed by the injection of a solution of ... diffusion of ions at the electrode/electrolyte interface DPV has demonstrated advantages of high sensitivity and the lowest detection limit by amplification of the electrochemical signal of polypyrrole ... potential 2.4 Construction of immunosensors Biotin was covalently bonded on copoly(py–pyNHP) by immersing the modified electrode with 50 ␮L of a mg mL−1 solution of biotin hydrazide in PBS pH 7.4 for...

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Improvement of Water Quality by Removal of Suspended Solids and High-speed Biological Filtration in Closed Water Body

Ngày tải lên : 05/09/2013, 08:40

... Páez-Osuna and A C Ruiz-Fernández Geochemical evidences of the anthropogenic alteration of trace metal composition of the sediments of Chiricahueto marsh (SE Gulf of California), Environmental ... removal of nitrogen should be considered, too References [1] Department of Water Environment, Ministry of the Environment, Japan July 2002 Outline of Administration of Water Environment P20 (In Japanese) ... influence of these disturbances was found Some sampling points were selected They are the outlet of plant, the outside of experimental area, points of horizontal level and 0.5, 1.0, 1.5 m of vertical...

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Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc

Ngày tải lên : 19/02/2014, 08:20

... variants, the modifications are not just single- point mutations, but include the incorporation of additional residues at the N-terminus and the removal of a disul de bond These findings suggest that ... secreted levels of a larger number of lysozyme variants, a highly significant correlation has been identified between the level of secreted protein and the thermostability of the native state of the protein ... activity, identified by the lysis of Micrococcus lysodeikticus cells, was detectable for both variants suggesting that the overall structure of the folded proteins is unlikely to differ significantly...

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Báo cáo khoa học: Mechanisms and kinetics of human arylamine N-acetyltransferase 1 inhibition by disulﬁram potx

Ngày tải lên : 07/03/2014, 02:20

... inhibition of aldehyde dehydrogenase by disul ram Chem Biol Interact 130–132, 93–102 Veverka KA, Johnson KL, Mays DC, Lipsky JJ & Naylor S (1997) Inhibition of aldehyde dehydrogenase by disul ram and ... formation of mixed disul des or covalent adducts 4904 on target proteins [3] The inhibition of target enzymes, such as aldehyde dehydrogenase, by DS often occurs through the modiﬁcation of an active ... signal This dose-dependent modiﬁcation was correlated with the dose-dependent inhibition of Table Effects of reducing agents on DS-dependent inhibition of NAT1 Conditions % of control activitya...

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Báo cáo "On the stability of the distribution function of the composed random variables by their index random variable " pdf

Ngày tải lên : 14/03/2014, 13:20

... constant independent of ε Indeed, by using Essen’s inequality (see [3]) we have 1 ρ(Ψ; Ψ1 ) C5 ε + C6 ε K1 ε T where K1 is a constant independent of ε (26) C4 ε (27) Nguyen Huu Bao / VNU Journal of ... occasion of 50th Anniversary of Department of Mathematics, Mechanics and Informatics, Vietnam National University References [1] Tran Kim Thanh, On the characterization of the distribution of the ... µA1 )|t| = C|t| (1 − |ϕ(t) − 1|) (do |ϕ(t) − 1| µF |t| ∀t) where C is a constant independent of ε and µF = Proof of Theorem 2.1 +∞ −∞ |x|dF (x) < ∞ For every N > and t ∈ R1, we have +∞ |ψ(t) − ψ1...

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Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR deﬁnes the domain boundaries and identiﬁes an unstructured insertion in the SH2 domain pdf

Ngày tải lên : 16/03/2014, 14:20

... peptide Experiments were performed at 298 K Solutions of 10– 25 lm SOCS3 in the cell were titrated by injection of a total of 290 lL of 80–200 lm of the gp130 peptide The heat of dilution of the ... levels Our identiﬁcation of the secondary structure and correct domain boundaries of SOCS3 will enable manipulation of SOCS3 function by rational mutagenesis This includes mutagenesis of the PEST ... made for SOCS3(22–185) Apart from ﬁve missing spin systems (Ser25–Ser28 and Gly170), 100% of 1HN, 100% of 15N (excluding 18 proline residues), 96% of 13Ca, 84% of 13Cb, 87% of 13C¢ and 84% of...

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Iron catalytic growth of prism shaped single crystal silicon nanowires by chemical vapor deposition of silane

Ngày tải lên : 16/03/2014, 15:06

... two-dimensional fourier transform of the outer layer of the triangle (indicated by arrow) depicting lattice spacing of Si(1 1) of 0.31 nm, Si(2 1) of 0.22 nm and Si(2 0) of 0.38 nm, respectively, the ... SiNWs with well -de ned structures at the lower temperature Summary In conclusion, by chemical vapor deposition of silane, the large-scale SiNWs were grown under the catalysis of Fe particles ... zone axis of single crystal Si Another SAED pattern was taken along [2 1] zone axis of single crystal Si (see Fig 2b, left inset) From the data above, we can conﬁrm the as-grown prism of single...

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Báo cáo khoa học: Mapping contacts between regulatory domains of skeletal muscle TnC and TnI by analyses of single-chain chimeras potx

Ngày tải lên : 16/03/2014, 18:20

... close localization of the N domain of TnC and the first half of the C terminus of TnI The sensitivity to degradation by proteases is related to the conformational flexibility of the protein substrate ... production of attached protein-reporters to facilitate purification or the investigation of activity [27], the creation of antibody chimeras [28], and the study of Table The ratios of the volumes (V) of ... regulatory subunit, and that the first half of the C terminus of TnI is important for this binding Structural information of the interaction of TnC and TnI were firstly provided by low-resolution scattering...

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