importance of tourism industry and its role in the philippine economy
Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
Ngày tải lên :
19/02/2014, 06:20
... research is that
understanding what causes the loss of appropriate
metal binding might be crucial for the understanding
of the role of amyloidogenic proteins in a number of
neurodegenerative disorders.
Experimental ... with
glycine alone was performed and no binding was observed.
In our hands, as others [54], the most reproducible data
was obtained from injections of the metal into a solution of
the protein. Typically ... important in the brain, there-
fore the role of copper binding or loss of its binding
could be related to specific cerebellar function(s) of
stefin B [18], which remains to be seen by more in vivo
studies.
Stefin...
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Tài liệu Báo cáo khoa học: Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites doc
Ngày tải lên :
20/02/2014, 11:20
... concerning the kinetics and properties of the
enzyme. More strikingly, we show a novel role of the PPase
enzyme in the development and molting process of A. suum
larvae in vitro.
Materials and methods
Parasites
Adult ... 14.635 lmolặmin
)1
ặmg protein
)1
. The inset in (A) repre-
sents the linear transformation of the curve. (B) Mg
2+
dependency was
determined as described in (A) in the presence of increasing concen-
trations ... determined by incubating the diluted recombin-
ant proteins in the standard reaction mixture in the presence
of increasing concentrations of PP
i
(0.05–0.5 m
M
)at55°C.
Data were fit to the appropriate...
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Báo cáo khoa học: Characterization of Trypanosoma brucei PEX14 and its role in the import of glycosomal matrix proteins pptx
Ngày tải lên :
23/03/2014, 17:21
... mislocalization of all
three enzymes in these cells clearly shows the involvement of
PEX14 in the targeting of PTS-1, PTS-2 and I-PTS proteins
to the glycosome.
To further analyse the effect of the destruction ... a binding constant K
d
of 2.75 l
M
, and that the
amino-terminal domains of the two peroxins were respon-
sible for this interaction.
The role of TbPEX14 in glycosome biogenesis received
further ... the
peroxin PEX14 is a component of a docking complex at the
peroxisomal membrane, where it is involved in the import of
matrix proteins into the organelle after their synthesis in the
cytosol and...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
Ngày tải lên :
14/02/2014, 19:20
... v-KIND KIND2 binding resides within
residues 702–744 of MAP2.
Residues 456–487 in the KIND2 domain of v-KIND
contain the core MAP2 binding module
To identify the core MAP2-binding site within the
KIND2 ... specific MAP2 binding resides
within the 32 residues (amino acids 456–487) of the
v-KIND KIND2 domain.
To examine whether the MAP2 binding core site of
v-KIND binds to intact MAP2 protein, we coex-
pressed ... under-
stood, the data obtained in the present study indicate
that the 43 residues (amino acids 702–744 in mice) that
reside in the middle region of the CD act as the
v-KIND binding core. The v-KIND-binding...
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Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx
Ngày tải lên :
18/02/2014, 18:20
... the basis of the
above findings, our research provides new insights into
the role of exon 9 in the regulation of apoptosis and
invasion in liver cell lines.
A
B
Fig. 7. Effects of occludin shRNA ... important roles in the induction of
mitochondria-mediated apoptosis and the inhibition of invasion, along with
the downregulation of matrix metalloproteinase expression. In addition, by
using the calcium ... Matrigel-coated inserts
were stained with crystal violet. The dots are stained cells on the bottom of the Matrigel-coated inserts. The number of invasive cells was
determined by counting the number of stained...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
Ngày tải lên :
19/02/2014, 07:20
... using
crotonyl-CoA as a substrate [15]. The gene encoding
this bifunctional protein was named AUH (‘AU bind-
ing homolog of enoyl-CoA hydratase’). The RNA-
binding activity of the human protein ... For the
kinetic characterization of AUH described in the work
at hand, AUH was overproduced in Escherichia coli as
a maltose binding protein fusion (MBP-AUH). The
complete AUH gene (producing ... gặL
)1
bovine serum albumin
and 10 lm 3-MG-CoA or glutaconyl-CoA. After incubation
at 37 °C for 60 min, the reaction was terminated by the addi-
tion of 2.5 lLof2m HCl. The samples were homogenized,
and...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
Ngày tải lên :
07/03/2014, 21:20
... that the interaction between VBARP
and Vpr is specific. The input panel represents the
amount of protein used in this assay indicating that an
equal amount of protein was used in all our samples
and ... con-
firmed that the cytoplasmic distribution of VBARP is
independent of the cycling stage of the cells.
Identification of biological function(s) of VBARP
using a siRNA assay
To examine the role of endogenous ... protein
containing a single ankyrin repeat that interacts with
HIV-1 viral protein R (Vpr) and we designated this
protein as Vpr-binding ankyrin repeat protein
(VBARP). This interaction was further...
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Expansion and its realization in the short story “the law of life” by jack london
Ngày tải lên :
29/01/2014, 14:44
... “hated”… At the beginning of the story, the main
character was introduced “an old Indian named Koskoosh” and the story is told from
the point of view of this character. In the text, one can find many ... a
randomly chosen text in terms of expansion to see how the findings can help to
understand some features of the text and the intention of the writer. In this thesis, the
text chosen is the ... realization in the short story The Law
of Life by Jack London has been finished. Now we shall sum up the results of the
study.
In chapter 2 of the study, the theoretical background of the most...
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Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt
Ngày tải lên :
19/02/2014, 17:20
... nm,
comparable to the inhibition of the aminopeptidase
cathepsin H (K
i
ẳ 30 nm ). In comparison with other
cystatins, cystatin F is a rather slow binding inhibitor
of the cathepsins, characterized ... Cystatins are generally rather non-
selective inhibitors. An interesting feature of cystatin
F is the 100-fold stronger inhibition of cathepsins F,
L, and V than of cathepsin S. Cathepsin S is ... process
regulating its inhibitory properties.
Screening of proteases for their inhibition showed
that cystatin F is different from other cystatins, both
in terms of specificity and strength of binding to the
target...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
Ngày tải lên :
07/03/2014, 12:20
... Prx
class, is their second redox-active cysteine, the resol-
ving cysteine (Cys-S
R
). In typical 2-Cys, S
p
at the N
terminus and S
R
at the C terminus of the protein
belong to different subunits and ... periods of time
(Fig. 3). As shown, the addition of stressors to the cul-
tures inhibits growth without killing the cells.
The hybridizing band in the northern analysis
showed the expected size of ... processes.
In this study we examined the role of Bcp2 in order
to increase the knowledge of the enzymatic activity
involved in the oxidative stress in S. solfataricus.
To detect differences in the response...
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
Ngày tải lên :
08/03/2014, 16:20
... 20 lL of the enzyme/inhibitor mixture to 180 lLof
substrate (4NPGlc), incubating for 30 min at 37 °C, and
measuring the rel ease of 4NP.
Protein assays and protein sequencing
Total protein in crude ... property
of enzymes involved in transformation of xenobiotics is
increased enzyme levels in the presence of xenobiotic
substrates, i.e. inducibility. Cloning the human cbg- 1 gene
(i.e. including the ... mLámin
)1
.The
following gradients were used: incubations containing
arbutin or salicin as substrate; 100% C initial, i ncreasing
D t o 2 0% (10 min), 50% ( 15 min), 100% (5 min), held at
100% (5 min);...
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Solar and Space Physics and Its Role in Space Exploration doc
Ngày tải lên :
14/03/2014, 10:20
... its charge in the broadest sense and to
examine both the fundamental roles of solar and space physics as aspects of scientific exploration and
the roles of the research in support of enabling ... are defined broadly and that include exploration to understand
the origin and evolution of the universe, the formation of planets and planetary systems, the origin and
extent of life, and the ... element of the fate theme, and it contributes
in key ways to many aspects of several components of the origins and evolution themes. In light of the
commission’s findings, the Committee on the...
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báo cáo hóa học: " Biomarkers of oxidative stress and its association with the urinary reducing capacity in bus maintenance workers" pptx
Ngày tải lên :
20/06/2014, 00:20
... only 10% of urate is excreted in the urine (the
remaining 90% being recirculated by the renal system
[3]), an increase of this antioxidant in blood will also
lead to an increase in urine. The fact ... H,
Savant Instruments Inc.). The final volume was deter-
mined by gravimetry, assuming that the entire methanol
was removed during the conc entration in the SpeedVac
and that the d ensity of the remaining ... (Institute for Work and Health) for their
help in the metal/creatinine analysis, and comments on the manuscript. The
medical team of the Institute for Work and Health (Prof Marcel-André Boillat,
Dr...
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Báo cáo khoa học nông nghiệp " Investigation of rice kernel cracking and its control in the field and during post-harvest processes in the Mekong Delta of Vietnam " docx
Ngày tải lên :
21/06/2014, 06:20
... of rice and to investigate the effect of tempering
on the increase in the mechanical strength of rice. This was the preliminary work mainly
to establish the methodology and feasibility of the ... extension workers, and collection of more da
training part of the program in the next 3 m
24
shared the existing information and experiences on rice cracking. The document prepared
(in Vietnamese ... 10: Increased mechanical strength of
the rice due to tempering
22
1. Institute Information
Project Name
Investigation of rice kernel cracking
and its control in the field and during...
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- 0
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- nội dung cụ thể cho từng kĩ năng ở từng cấp độ
- xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct
- phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ
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