implementing a characterization of genre
Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx
Ngày tải lên :
14/02/2014, 03:20
... was maintained by
using beryllium metal foil to seal the X-ray optical path.
Table 2. Summary of DSC data, suggested results, and additional characterizations
sample no. peak onsets (°C) peak ... nitrogen
purge.
Evolved Gas Analysis. Simultaneous TG/MS and TG/gas
chromatography (GC)/MS analysis were performed on several
samples. A split was used to send a fraction of the evolved
gases to a quadruple mass ... TGA analysis was performed on several
samples using a TA 2950 TGA with a platinum pan. A nitrogen
atmosphere was used for each trial. Some analyses were
performed on a Perkin Elmer-7 TGA with an...
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Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt
Ngày tải lên :
18/02/2014, 06:20
... Meyer-Klaucke for data collection and assis-
tance in data evaluation. The assistance of T. Pavkov
(Institute of Chemistry, University of Graz) in the
acquisition of CD and DLS data is gratefully acknowl-
edged. ... B. xenovo-
rans LB400 through a PCR with GAGCGG
CATATGGA
AATCAAACCGAAGGTTCGCGA and GAGCGG
CATA
TGGAAATCAAACCGAAGGTTCGCGA as the forward
and reverse oligonucleotide primers, respectively. The
primers ... depletion
of a molar equivalent of dissolved O
2
. HPLC analysis
of the products of the enzymatic transformation
revealed that Bxe _A2 876 catalyzed breakdown of the
b-diketone substrate via oxidative carbon–carbon...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
Ngày tải lên :
18/02/2014, 08:20
... molecular basis of enzyme thermosta-
bility. J Bacteriol 185, 4038–4049.
20 Nanba H, Yajima K, Takano M, Yamada Y, Ikenaka
Y & Takahashi S (1997) Process for producing d-N-car-
bamoyl -a- amino acid. ... molecular mass
was also estimated by SDS–PAGE.
Characterization and comparative analyses of
HYD
Js
and HYD
Bp
The optimal temperature for activity of HYD
Js
with d-p-
HPH as substrate was determined ... Bommarius AS, Schwarm M & Drauz K (1998) Biocatal-
ysis to amino acid-based chiral pharmaceuticals - exam-
ples and perspectives. J Mol Catal B-Enzym 5, 1–11.
14 Liljeblad A & Kanerva LT...
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Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx
Ngày tải lên :
18/02/2014, 17:20
... 2008)
doi:10.1111/j.1742-4658.2008.06352.x
Cone snails, a group of gastropod animals that inhabit tropical seas, are
capable of producing a mixture of peptide neurotoxins, namely conotoxins,
for defense and predation. Conotoxins are mainly ... and
sequence of dermorphin, a novel opiate-like peptide
from the skin of Phyllomedusa sauvagei. Int J Pept
Protein Res 17, 275–283.
10 Kuwada M, Teramoto T, Kumagaye KY, Nakajima K,
Watanabe T, Kawai T, ... Kawakami Y, Niidome T,
Sawada K, Nishizawa Y et al. (1994) Omega-agatoxin-
TK containing D-serine at position 46, but not syn-
thetic omega-[L-Ser46]agatoxin-TK, exerts blockade of
P-type calcium...
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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx
Ngày tải lên :
19/02/2014, 02:20
... TGGCCAGATCTAAAAAAGAGGT
2fw ATCCCAGGAAACACCAGTAGA
10rev ATTGTTTTCTCTCAAGACCCAA
TaqMan probe T1 ACACCACAGCAAGGGAGAAGCAAAG
18Sfw CGCCGCTAGAGGTGAAATTC
18Srev TCTTGGCAAATGCTTTCGCT
TaqMan probe 18S TGGACCGGCGCAAGACGGAC
AB
Fig. ... rev,
reverse.
Sequence
ACMSD cloning: primer
1fw CGCTCGAGATGAAAATTGACATCCATA
GTCAT
11rev AAAGCTGAGCTCCATTCAAATTGTTTT
CTCTCAAG
4fw TTCTCGAGATGGGAAAGTCTTCAGAGT
GGT
ACMSD real-time PCR: primer and probe
1 ⁄ 3fw TGGCCAGATCTAAAAAAGAGGT
2fw ... Ala was
carried out using the QuickChange kit (Stratagene, La Jolla,
CA, USA). Mutagenic primers were: 5¢-CGCTCGAGA
TGAAAATTGACATC
GCTAGTCATATTCTACC-3¢ and
its complement for His6Ala; 5¢-GACATCCATAGT
GCT
ATTCTACCAAAAGAATGGCC-3¢...
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Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx
Ngày tải lên :
19/02/2014, 05:20
... oxyheme
appeared at 540 and 579 nm. Then, a broad band
appeared at around 660 nm, and was maximal 9–12 min
after initiation of the reaction. The spectral features of
the final reaction mixture were analogous, ... T, Zhang X, Sun D,
Sato M, Sasahara M, Kayama T, Ikeda-Saito M &
Yoshida T (2000) Histidine 20, the crucial proximal
axial heme ligand of bacterial heme oxygenase Hmu O
from Corynebacterium ... vanished
after 30 min, and instead, an absorption peak
appeared at 637 nm, suggesting the formation of
CO–verdoheme. The 637 nm band disappeared gradu-
ally and was replaced by a new broad band...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
Ngày tải lên :
19/02/2014, 06:20
... GTT GGA ATT CCA TCA TCA TCA
TCA TCA TCA GGG CAC GAC ACA CAT CCC C; and
reverse primer, GAT CGG TAC CTC ATT ACA GAG
GAG GGA TAT GGG GAA C. The PCR reaction was
done as described above. The amplified ... T. reesei DNA
with the following primers: forward, GGG GAC AAG
TTT GTA CAA AAA AGC AGG CTA TCA TGC TGT TGT
CAG GTC CCT CTC G; and reverse, GGG GAC CAC
TTT GTA CAA GAA AGC TGG GTC A GT GGT GGT
GGT ... oxidase, Japanese patent 61115488.
37 Yamada Y, Tawara Y & Yoshika H (1983) Production
of heat-resistant polyphenol oxidase, Japanese patent
60062980.
38 Abdel-Raheem A & Shearer CA (2002)...
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Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx
Ngày tải lên :
19/02/2014, 06:20
... 5¢-CTTTATTTTCAGGGCGCCATGAAGCGCG
CAAGACCGTCTGAA-3¢ and a reverse oligomer 5¢-AGCT
CGAATTCG GATCCGGTACCTCAGAAGGTAGACAG
CAGAACC-3¢. For derivitization with TMR, a Gly-Gly-Cys
sequence was introduced at the C-terminus ... addition to an N-terminal Nco1 cloning site.
The forward o ligomer 5 ¢-TCCGAAACCAGCG GCCGCTT
TATCGCGTTA AAAC CGGT GATCAA ACCCC -3¢ and the
reverse oligomer 5¢-GTAGGCCTT TGAATTCCTCAAAA
AGTGCGGCTCGAT-3¢ ... pentamer give a total surface
area of 81 000 A
˚
2
with 30% ( 24 000 A
˚
2
) as con-
tact area. Thus, a large amount of the available surface
area of the molecule is buried upon pentamerization,
increasing...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
Ngày tải lên :
19/02/2014, 07:20
... C)AATTTC-3¢ PsCBL (degenerate forward)
45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ PsCBL (degenerate reverse)
55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward)
65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR ... T)GC(C ⁄ G ⁄ T)AAGGT-3¢ PsCIPK (degenerate forward)
25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate reverse)
35¢-CTTAT(C ⁄ G)AACAAGGAA (A ⁄ C)AATTTC-3¢ PsCBL ... Transgenic
evaluation of activated mutant alleles of SOS2 reveals a
critical requirement for its kinase activity and C-term-
inal regulatory domain for salt tolerance in Arabidopsis
thaliana....
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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
Ngày tải lên :
19/02/2014, 07:20
... primers: gatggatcccatATGGGTG
TTGAAGTTGTA annealing around the start codon of the
Ppi1 ORF and gactcgagATTAGTCGACTTCTTACGC
annealing just before the putative transmembrane domain
(capital letter ... Soave C & De Michelis
MI (2002) A novel interaction partner for the C-termi-
nus of Arabidopsis thaliana plasma membrane H
+
-ATP-
ase (AHA1 isoform): site and mechanism of action on
H
+
-ATPase ... H
+
-ATPase
activity was evaluated as the difference between total activ-
ity and that measured in the presence of 100 lm vanadate
(less than 10% of total activity at pH 7; less than 5% of
total activity...
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Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt
Ngày tải lên :
19/02/2014, 07:20
... 5¢-CTTTAACTTGTTGGGCACTGG
CATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCC
CA-3¢ along with the adaptor primers: AP1 (5¢-GTAATAC
GACTCACTATAGGGC-3¢) and AP2 (5¢-ACTATAGGG
CACGCGTGGT-3¢).
Nested PCR was carried ... obtain the full length sequence:
OP5, 5¢-GACTGTAACGGTCATGGYACMAYGT-3¢;
OP6, 5¢-GATGAAAATCCTAACCTCTCCCCCGCACAG-
3¢; OP7, 5¢-ACTGCACCTACGGCGGGTCGTTGGTACG
TG-3¢; NP4, 5¢-GACACCGTAGGTTGAGCCGCCAATC
GTCCC-3¢; ... PCR was performed in 50 lL containing 1 ng
of genomic DNA as template, 0.2 mm dATP, dCTP, dGTP
and dTTP, 0.2 lm of upstream primer (OP17: 5¢-GA
AAAACCATGGTGAATGAATACCAAGCGACT-3¢ ) and
downstream...
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Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
Ngày tải lên :
19/02/2014, 16:20
... pGL3e:Prm 3a
AP)1
*,
pGL3b:Prm3ab
AP)1
*, pGL3e: Prm3ab
AP)1
*, pGL3b:
Prm3aa
AP)1
*, pGL3e:Prm3aa
AP)1
*, pGL3b:Prm3aaa
AP)1
*
and pGL3e:Prm3aaa
AP)1
*.
Mutation of the Oct-1 element with the sequence aaA
TGCa to aaTTCCa ... Witte DP & Grabowski GA (1998) Isola-
tion and characterization of the human prosaposin pro-
moter. Gene 218, 37–47.
51 de Grazia U, Felli MP, Vacca A, Farina AR, Maroder M,
Cappabianca L, Meco ... to aaTTCCa (core bases shown in uppercase letters)
centered at )123 within Prm3 was performed using the
mutator primers Kin175 (5¢-CACCAGAGCTACTTACA
CTGAATTCCAGAATAATCACAAGCAAATC-3¢; sense
primer)...
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![Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc](https://media.store123doc.com/images/document/14/br/vp/medium_vpv1392870032.jpg)
Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc
Ngày tải lên :
20/02/2014, 11:20
... alkyla-
tion leading to a depletion of mtDNA in intact cells
(Fig. 1). Here we report the synthesis and characterization
of a novel mitochondria-targeted alkylating reagent and
show that it alkylates ... presence of relaxed-circular mtDNA after digestion
with ClaI may indicate alkylation of the restriction site as
untreated mtDNA, or mtDNA isolated from mitoDC-81-
treated mitochondria, was always ... entirely wrap mtDNA and cause a marked
increase in nuclease resistance in vitro [54]. Similar
targeting of PNAs to mitochondria also failed to show
inhibition of mtDNA replication in intact cells...
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Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
Ngày tải lên :
21/02/2014, 00:20
... hemolymph of P. jacquemontii by affinity
chromatography. It yielded a 2000-fold increase in specific
activity. Analysis of the lectin on SDS/PAGE gave a single
band at apparent molecular mass of 34 kDa.
The ... purification.
Erythrocyte preparation
Blood for HA assay was prepared as described by
Ravindranath et al. [12].
Hemagglutination assay
Hemagglutination assays were performed in microtiter
plates (Falcon) as recommended ... & Paulson, J.C.
(1985) Purification and characterization of an O-acetyl sialic acid
specific lectin from a marine crab Cancer antennarius. J. Biol.
Chem. 260, 8850–8856.
13. Kawai, T. Kato, A. ...
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Tài liệu Báo cáo Y học: Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf
Ngày tải lên :
21/02/2014, 01:21
... phosphatase gene. Astandard
BLAST
search, with AF164795 as the query, was
performed against the nonredundant databases at the NCBI site. In that way, the gene of the human phosphohistidine phosphatase ... gene.
EXPERIMENTAL PROCEDURES
Materials
Phosphoamidate was prepared according to the method of
Wei and Matthews [4]. Suc-Ala-His-Pro-Phe-pNA was
purchased from Bachem AG, Switzerland. Malachite green
reagent ... traced
by its absorbance at 315 nm, appeared as a peak at 0.33
M
MDEA and was essentially stable in this buffer at +4 °C
for at least 4 months. The phosphate content of the
phosphopeptide was analysed...
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