immunotherapy of hiv infection dendritic cells as targets and tools

báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx

Ngày tải lên : 18/06/2014, 15:20

... was at least as efficient as an ubiquitous promoter (data not shown) In contrast to the desmin promoter, the C512 promoter was also active in monocyte-derived DC and LC (around 10% of E-GFP+ cells) ... and non-adherent cells in culture were harvested at day and transduced by LV-VSVG-PGK at an MOI of 18 TU/ml and at a fixed concentration of × 106 cells/ ml, in RPMI 1640 After hours at 37°C, cells ... concentration of 2–5 × 106 of cells per 200–500 μl of medium After hours at 37°C, cells were placed in complete medium and analysed by flow cytometry between day and day 60 Transduction of CD34-pDC...

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Báo cáo y học: "Topical application of entry inhibitors as "virustats" to prevent sexual transmission of HIV infection" pptx

Ngày tải lên : 13/08/2014, 05:21

... most instance of HIV transmission [37,38] In fact both dextran sulfate and cellulose sulfate can actually increase HIV infectivity in vitro [39,40], and plasma levels of HIV were increased in persons ... of promising candidates under development, including inhibitors of entry as well as inhibitors of other elements of the viral life cycle[12] Unfortunately, the field (and public perceptions of ... A number of small molecule allosteric inhibitors of CCR5 have been developed for systemic administration; one of these, maraviroc, has been approved for treatment of HIV infection, and another,...

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báo cáo hóa học:" When masculinity interferes with women’s treatment of HIV infection: a qualitative study about adherence to antiretroviral therapy in Zimbabwe" pdf

Ngày tải lên : 20/06/2014, 08:20

... between socially constructed notions of masculinity and HIV Men’s fear and denial of HIV To mediate the links between social constructions of masculinity and husbands’ interference with their wives’ ... between masculinity and HIV and how this has led many men in this context to develop a profound fear of any association with HIV, preventing them from getting tested and disclosing their HIV status: ... positive and knew of their husbands’ fear of HIV were often afraid of telling their husbands about their HIV seroconversion: I know a certain lady who attended these HIV/ AIDS functions and decided...

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Báo cáo y học: "Fosamprenavir or atazanavir once daily boosted with ritonavir 100 mg, plus tenofovir/emtricitabine, for the initial treatment of HIV infection: 48-week results of ALERT" potx

Ngày tải lên : 10/08/2014, 05:21

... discretion of the investigators However, usage of lovastatin and simvastatin was prohibited, and atorvastatin and fluvastatin were to be used only on a precautionary basis in view of some potential ... Society (IAS) treatment guidelines recommend ritonavir-boosted FPV as a recommended PI-based treatment for the initial treatment of HIV infection [23] and the British HIV Association (BHIVA) treatment ... effects of TDF [7] As of January 2008, ATV/r100-based regimens are considered first-line PI regimens by DHHS HIV treatment guidelines [21], as recommended PI regimens by IAS guidelines [23], and as...

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Báo cáo y học: "Determinants of late disease-stage presentation at diagnosis of HIV infection in Venezuela: A case-case compariso" ppt

Ngày tải lên : 10/08/2014, 05:21

... was a decreasing trend for late presentation with increasing knowledge of HIV- transmission and awareness of the PNSIDA (Table 2) Awareness of existence and free availability of HIV testing was ... correct mode of transmission (range 0–15) Knowledge of existence, availability and prices of HIV- tests as well as ART was assessed A PNSIDA awareness score was calculated adding one Page of 12 (page ... contact with commercial sex workers and previous occurrence of STIs was recorded during interview Knowledge of HIV/ AIDS Knowledge of HIV/ AIDS before HIV- diagnosis was assessed during the interview...

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Báo cáo y học: "Structure of HIV-1 quasi-species as early indicator for switches of co-receptor tropis" docx

Ngày tải lên : 10/08/2014, 05:21

... marks the 10% detection rate of standard Trofile® assay Naming was adopted from [21] Page of reliable detection of X4 minorities in test mixtures by the standard Trofile® assay at concentrations down ... agreement of the two Trofile® classes R5 and DM (dual/mixed) with predicted X4 tropic fractions of below and above 0.1, respectively This agreement holds for all four patients and all time points, and ... of Figure Development of quasi-species Development of HIV- 1 quasi-species in patients Every dot represents a combination of two tropism predictions for the same V3 sequence: one prediction based...

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Báo cáo y học: " Possible transmission of HIV Infection due to human bite" potx

Ngày tải lên : 10/08/2014, 05:22

... PEP, as salivary transmission of HIV is rare and negligible On 29 March 2010, four weeks after the incidence.(Mr A) was hospitalized and his HIV Duo test was weakly positive and his viral load was ... sites and bold letter indicates variation ♦Starts C2 region of HIV1 gp120 followed by V3 region Starts at 296 and end at 330 positions of HIV DNA by amplifying C2-V3 Region of HIV1 C envelope gene and ... detection of HIV infection for monitoring responses to ARV therapy Conclusions Our observations revealed transmission of HIV infection from the smear of non-contaminated saliva of [Mr.X] on the raw and...

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báo cáo khoa học: " Predictors of HIV infection and prevalence for syphilis infection among injection drug users in China: Community-based surveys along major drug trafficking routes" pps

Ngày tải lên : 11/08/2014, 18:20

... Macao, and Taiwan, had reported cases of HIV/ AIDS among drug users from 1989 to 2004 Yunnan reported the highest number of annual HIV/ AIDS cases in mainland China [7] Yunnan's proximity to one of ... Total N may not equal 689, because of missing data; N: the number of participants being tested for HIV; †: %: the prevalence of HIV infection; HIV+ : the number of HIV positive Results Socio-demographic ... discrimination associated with drug use and HIV/ AIDS, fear of arrest due to illegal practice, knowing a positive result, a lack of coping skills, and knowledge of HIV risks are the other reasons for...

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báo cáo khoa học: " High prevalence of HIV infection among homeless and street-involved Aboriginal youth in a Canadian setting" potx

Ngày tải lên : 11/08/2014, 18:20

... positive for HIV, of whom (46.7%) were of Aboriginal ethnicity As shown in Table 1, Aboriginal ethnicity was associated with HIV infection (odds ratio [OR] = 2.87, 95%CI: 1.02 – 8.09), as was injection ... concept and design and was responsible for the composition of the manuscript The statistical analysis was conducted by KL and the interpretation of the results was performed by BM, CL, EW, JM and ... between the age of 14 and 26, self-reported use of illicit drugs other than or in addition to marijuana in the past 30 days, and the provision of informed consent The study has been approved...

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Báo cáo y học: " Kaposi''''s sarcoma of the hand mimicking squamous cell carcinoma in a woman with no evidence of HIV infection: a case report" pptx

Ngày tải lên : 11/08/2014, 21:22

... presence of disease Figure regarded Kaposi's sarcoma carcinoma A primaryas a squamous cell on a hand, which was first A primary Kaposi's sarcoma on a hand, which was first regarded as a squamous ... HHV8 infection [4] B Figure spaces and sarcoma consisting of angiomatoid 40 A) Kaposi'sabundant spindle-shaped cells HE × vascular A) Kaposi's sarcoma consisting of angiomatoid vascular spaces and ... angiogenesis and replication of the spindle cells typical of KS [6,7] The presence of HHV8 infection is now considered essential for the development of KS However, the incidence of HHV8 infection is far...

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Báo cáo y học: " HIV interactions with monocytes and dendritic cells: viral latency and reservoirs" pptx

Ngày tải lên : 12/08/2014, 23:21

... spread HIV- 1 infection and maintain viral reservoirs HIV infection of DCs HIV- 1 is capable of directly infecting different DC subtypes (known as cis infection) , but at a lower efficiency than HIV- 1's ... trans infection The most interesting aspect of HIV- 1 infection in DCs is the ability of the cells to act as mediators of trans infection of activated CD4+ T cells, which is the most productive ... extensive understanding of the mechanisms of HIV latency [1,2] Given that monocytes and DCs have been implicated as HIV- 1 reservoirs using in vitro and ex vivo models of viral infection (Table...

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Báo cáo y học: "Semen-mediated enhancement of HIV infection is donor-dependent and correlates with the levels of SEVI" docx

Ngày tải lên : 13/08/2014, 01:20

... of HIV infection by SE relative to that measured for the corresponding PBS control (C) Metabolic activities of cells analysed in B (D) Effect of low concentrations of SE on HIV infection R5 HIV- 1 ... further support an important role of SEVI in SE-mediated enhancement of HIV- 1 infection and thus in the transmission of HIV/ AIDS Page of 12 Results Semen boosts HIV- 1 infection under non-cytotoxic ... transmissions of HIV/ AIDS, however, the enhancing effect of SE on HIV- 1 infection remains poorly defined and controversial One reason is the lack of standardized methods addressing the high cytotoxicity of...

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Báo cáo y học: " The pathogenesis of HIV infection: stupid may not be so dumb after all" ppsx

Ngày tải lên : 13/08/2014, 09:20

... activation and viremia appear together, as in the case of HIV- infected humans and SIVinfected macaques, disease occurs Modulation of TCRCD3 may help prevent activation of the immune system Page of (page ... stimulation and activation of the remaining cells Proinflammatory cytokines are also likely to play a role: CD8+ T -cells are activated and not infected with HIV/ SIV, B -cells and NK cells are also ... progressive depletion of the remaining CD4+ T -cells Much of the new data in support of this concept comes from non-human primate models of HIV infection More than 30 monkeys and apes are naturally...

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Báo cáo khoa học: "Effect of fungal infection on leaf gas-exchange and chlorophyll fluorescence in Quercus ilex" doc

Ngày tải lên : 08/08/2014, 14:21

... the presence of cankers and their length was recorded The presence of mycelia, pycnidia and conidia was recorded and canker size was measured every two months throughout the study Vascular cambium ... growth and sporulation of C parasitica was abundant, 168 B El Omari et al and the growth of the canker was 19% lower in E-plants (figure 1) Visual symptoms of Q ilex infected by C parasitica ... the effect of infection on gas-exchange was not detected in spite of the progress of the disease in inocu- 28 3.2 Gas exchange 98 Figure Canker length progression in undisturbed plants and plants...

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Báo cáo y học: "The impact of HIV-associated lipodystrophy on healthcare utilization and costs" doc

Ngày tải lên : 10/08/2014, 05:21

... HAART has reduced morbidity and increased the life expectancy of persons infected with HIV [13,14], as reflected by improved disease measures (such as increased CD4 count and lower viral loads, as ... accumulation and metabolic abnormalities in the HIV- infected population is not as compelling as in non -HIV infected populations, and lipoatrophy also has been associated with metabolic and cardiovascular ... among the HIV- infected community have demonstrated a significant relationship between worsening disease status (as represented by decreasing CD4 count and increasing HIV viral load) and increased...

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Báo cáo y học: " Successful treatment of HIV-associated multicentric Castleman’s disease and multiple organ failure with rituximab and supportive care: a case report" potx

Ngày tải lên : 11/08/2014, 14:21

... reflecting better understanding of HIV- associated disease, the availability of new combination antiretroviral therapy, the improved care of HIV patients both outside and within the ICU, and protective ... 102(8):2786-8 Casquero A, Barroso A, Fernandez Guerrero ML, Gorgolas M: Use of rituximab as a salvage therapy for HIV- associated multicentric Castleman disease Ann Hematol 2006, 85(3):185-7 Casalino ... Successful treatment of HIV- associated multicentric Castleman’s disease and multiple organ failure with rituximab and supportive care: a case report Journal of Medical Case Reports 2010 4:32...

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Báo cáo y học: "Effects of bone marrow-derived cells on monocrotaline- and hypoxia-induced pulmonary hypertension in mice" pps

Ngày tải lên : 12/08/2014, 15:20

... both MCTp and male BMDCs, about 0.2% of total DNA was of male origin (Fig 5, panels A and B), demonstrating that the graft was able to localize in the lung of these animals but in very few cells ... containing antiproteases (1 µM leupeptin and µM pepstatin A) and CHAPS (20 mmol/ L) and the homogenate was centrifuged at 3,000 g for 10 at 5°C Western Blot analysis was then performed as previously ... alter the development of PH as assessed by RVSP, RV/LV+S, and pulmonary vessel muscularization Irradiated BMDCs increased the percentage of muscularized pulmonary vessels and the RV/LV+S index...

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Báo cáo y học: " Inhibition of HIV-1 gene expression by Ciclopirox and Deferiprone, drugs that prevent hypusination of eukaryotic initiation factor 5A" potx

Ngày tải lên : 12/08/2014, 23:22

... decrease in HIV infections [1] Studies of the HIV- 1 life cycle led to the development of drugs targeting viral proteins important for viral infection, most notably reverse transcriptase and protease ... Scheme inhibition of HIV- 1 infection by CPX and DEF Scheme for inhibition of HIV- 1 infection by CPX and DEF The model integrates results presented in this paper for the early phase of drug action ... phase I the drugs cause a reduction in the expression of HIV proteins, some of which are necessary to prevent apoptosis of HIV infected cells Apoptosis of infected cells takes place during phase...

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Báo cáo y học: "Phylodynamics of HIV-1 Circulating Recombinant Forms 12_BF and 38_BF in Argentina and Uruguay" pps

Ngày tải lên : 12/08/2014, 23:23

... covered the protease (PR) and part of the reverse transcriptase (RT) genes (nucleotides 2266-3705 relative to the HXB2 clone), encompassing the recombinant fragments of the CRF12_BF and CRF38_BF ... years), and the mode and rate (r, years -1 ) of population growth for the CRF12_BF and CRF38_BF strains were estimated using BEAST v1.4.7 [36,37] Evolutionary and demographic parameters of CRF12_BF ... X-axis of each graph) BSP represent estimates of effective number of infections (Y-axis; log10 scale) through time (X-axis; calendar years) Median (solid line) and upper and lower 95% HPD (dashed...

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