immunocytochemistry to study myogenesis in zebrafish
Báo cáo khoa học: Death-associated protein kinase (DAPK) and signal transduction: fine-tuning of autophagy in Caenorhabditis elegans homeostasis doc
Ngày tải lên :
29/03/2014, 08:20
... responding to growth factors and amino acid signaling, (b) AMP-activated protein kinase, which activates autophagy through the inhibition of mTOR signaling, (c) Bcl-2, which binds to Beclin and inhibits ... role in diverse neurodegenerative diseases Aging Fig Schematic diagram of autophagy signaling pathway in C elegans Autophagy inducing- and inhibiting signaling pathway in C elegans Plain lines ... death-associated protein kinase (DAPK) in the regulation of autophagy Fine-tuning of autophagy in C elegans homeostasis is particularly interesting DAPK is a serine ⁄ threonine kinase originally isolated...
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Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
Ngày tải lên :
06/08/2014, 19:21
... pathways Amino-acid pools and other nutrients activate TOR signaling, which then results in increased translation following activation of the protein kinase S6K and the translation initiation factor ... cellular amino-acid pools Specifically, Fuchs et al [7] show that mutations in daf-2/IGF1R, daf-28/insulin and ife-2/eIFE4 cause an increase in amino acid levels, especially branched-chain amino acids ... branched-chain amino acids are known to stimulate protein synthesis and inhibit protein degrada tion in higher eukaryotes [10], a phenomenon mediated by the TOR (target of rapamycin) pathway...
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Báo cáo sinh học: "Robust and specific inhibition of microRNAs in Caenorhabditis elegans" doc
Ngày tải lên :
06/08/2014, 19:21
... protocols are available to effectively and conveniently knockdown miRNA function transiently To inhibit miRNA expression in C elegans, Zamore’s group injected 2’-oxymethyl oligonucleotides into ... similar to those seen in the respective loss of function mutant strains of lin-4 and let-7 For example, progeny of animals injected with antisense oligonucleotides against lin-4 show delayed exit in ... to the target microRNA is injected into the syncytial gonads of C. elegans The transformed progeny are selected by the presence of rhodamine In these progeny the antisense oligonucleotides bind...
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Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-fluorouracil in Caenorhabditis elegans docx
Ngày tải lên :
30/03/2014, 01:20
... strongly in neurons and the pharynx, but weakly in the intestine Scale bars: 100 lm (B) In vitro enzymatic assays of three uridine kinase homologs using analytical HPLC No proteins exhibited uridine ... uridine kinase and OPRT converge, resulted in high 5-FU resistance As C29F7.3 is expressed in the hypodermis and intestine, both UP ⁄ UMPK in the hypodermis and OPRT ⁄ UMPK in the intestine are ... 4724 In vitro enzymatic assays To induce the MBP–UPP1 fusion protein, 0.3 lm IPTG was added to the culture Induced fusion proteins were purified using amylose resin (New England Biolabs), according...
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Báo cáo khoa học: Stage-specific activation of MIG-17⁄ADAMTS controls cell migration in Caenorhabditis elegans docx
Ngày tải lên :
30/03/2014, 04:20
... suggesting that the C-terminal histidine tag remained in both the proform and activated form in the recombinant enzyme after its secretion into the culture medium (Fig 2B) To determine the N-terminal ... with which to study the function of ADAMTSs during organogenesis in vivo In this study, we isolated recombinant MIG-17 and determined the amino acid sequence of the N-terminal neo-epitope of the ... membranes In mammals, tissue inhibitor of proteinases-3 inhibits ADAMTS proteases [27,28] Moreover, the extracellular glycoprotein papillin inhibits an ADAMTS in Drosophila [29] Genes encoding related...
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Báo cáo sinh học: "A global analysis of genetic interactions in Caenorhabditis elegans" doc
Ngày tải lên :
06/08/2014, 18:21
... linked by seven SGI interactions to the bar-1 query gene, 11 fine genetic interactions, 36 co-phenotype links, three coexpression links, and one protein-protein interaction link To further investigate ... analyzed involve receptor tyrosine kinase signaling Interestingly, let-60 and sem-5 each interact with all of the query genes but not interact with clk-2, suggesting that they are common mediators ... al 8.3 to one of six signaling pathways specific to metazoans, including the insulin, epidermal growth factor (EGF), fibroblast growth factor (FGF), Wingless (Wnt), Notch, and transforming growth...
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Báo cáo y học: "Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails" ppsx
Ngày tải lên :
14/08/2014, 14:21
... or budding yeast (cutoff BLASTP score E = × 10-20) in each bin is indicated as in (a) In all panels, the red dotted line indicates the average of all the genes, and the blue dotted lines indicate ... but not in neurons or the intestine, while 'Intestine' was genes expressed in the intestine, but not in neurons or muscles Xboxes were searched for using MEME and MAST [60] based on the definition ... FLAG-PAB-1 in ventral cord motor neurons was weak in the acr-5::PABP strain compared to that in sensory neurons in the che-2::PABP strain As a measure of the functional integrity of FLAG-PAB-1-expressing...
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Báo cáo y học: "Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution" pdf
Ngày tải lên :
14/08/2014, 17:22
... protein/actin interacting protein rab-8 + rab-10 GTP-binding protein SEC4, small G protein superfamily, and related Ras family GTP-binding proteins B0495.2 + ZC504.3 Protein kinase PITSLRE and related kinases ... dsRNA-expressing bacterial strain targeting lin-31 (data not shown), showing that the observed dilution effect appears not to be specific to the diluting dsRNA-expressing strain interactions Taken together ... ribosomal protein P2 C28H8.4 + erd-2 ER lumen protein retaining receptor lin-12 + glp-1 Member of the Notch/LIN-12/glp-1 transmembrane receptor familya C13G3.3 + W08G11.4 Serine/threonine protein phosphatase...
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Báo cáo y học: "Identification of ciliary and ciliopathy genes in Caenorhabditis elegans through comparative genomics" pptx
Ngày tải lên :
14/08/2014, 17:22
... research Mitogen-activated protein kinase kinase kinase kinase isoform + deposited research Intraflagellar transport 80 homolog Fas apoptotic inhibitory molecule reports - reviews C02H7.1 *Genes in these ... R126.9 Data mining and gene finding HMMER and motif finding The engineering procedure was as described in our previous publications [53,54] Briefly, the GFP coding sequence was 'stitched' together ... is, therefore, required for building and maintaining nematode ciliary structures DAF19 is orthologous to human RFX transcription factors, which bind to cis-regulatory elements called X-box motifs...
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Báo cáo khoa học: Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle ppt
Ngày tải lên :
05/03/2014, 23:20
... suggesting that prejunctional P2 receptors were not involved in the facilitating effects of cADPR In contrast, the inhibitors of intracellular cADPR receptors 8-Br-cADPR (80 lm) and ryanodine (50 ... J Clin Gastroenterol 39, S184–S193 31 Lecci A, Capriati A, Altamura M & Maggi CA (2006) Tachykinins and tachykinin receptors in the gut, with special reference to NK2 receptors in human Auton ... signal transduction pathways in response to cADPR transported into the cytosol via membrane-bound CD38 Thus, similar to studies in some cell lines [9,10], the present study suggests that extracellular...
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Báo cáo Y học: Dystrobrevin requires a dystrophin-binding domain to function in Caenorhabditis elegans doc
Ngày tải lên :
08/03/2014, 23:20
... H2 is involved in the interaction with Dys-1 in vitro Within this domain, the first half seems to be particularly critical as deletions infringing on this side lead to a decrease in binding Constructs ... Dyb-1 binds to Dys-1 (Fig 4) Similar results were obtained when a shorter Dys-1 fragment (amino acids 3402–3674) encompassing the syntrophin-binding domain and the coiled-coil domain (corresponding ... dystrobrevin protein will remain unknown, the only way of making functional investigations will continue to be through complementation of mutations A preliminary in vitro study on Dys-1–Dyb-1 interactions...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
Ngày tải lên :
16/03/2014, 00:20
... with lm SYTOX Green in NaCl ⁄ Pi for in the dark After addition of peptide, the increase in fluorescence, owing to the binding of the dye to intracellular DNA, was measured at 37 °C in a microplate ... 0.73 0.98 20 cin, and rifampicin [a fractional inhibitory concentration (FIC) £ 0.5 indicates synergy; see Experimental procedures] To obtain insights into the mode of action underlying the synergistic ... Glucosamine-fructose-6-phosphate aminotransferase 6707 Glucosamine-fructose-6-phosphate aminotransferase 2702 5103 5603 Trigger factor Outer membrane protein W Dihydrolipoyllysine residue succinyltransferase...
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Báo cáo khoa học: Lysosomal localization of GLUT8 in the testis – the EXXXLL motif of GLUT8 is sufficient for its intracellular sorting via AP1- and AP2-mediated interaction docx
Ngày tải lên :
16/03/2014, 02:20
... third study detected GLUT8 only in differentiating spermatocytes but not in mature spermatozoa [15] Heterotetrameric adaptor protein (AP) complexes mediate membrane protein sorting in the secretory ... marker protein LAMP1, a finding that is in agreement with our in vitro binding data Targeting of GLUT8 in the absence of AP1 and AP2 In order to investigate the contribution of AP adaptors, most ... detected by incubating cells with the anti-haemagglutinin IgG in cell culture prior to fixation (in green) The haemagglutinin antibody recognizes plasma membrane GLUT8 via a haemagglutinin epitope that...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
Ngày tải lên :
16/03/2014, 02:20
... IgG TN Fα In s TN Fα + C In s on Input 2.5 1.5 ** * 0.5 TN Fα + TN Fα s In In C s on Foxa2 binding normalized to input (arbitary units) B Foxa2 binding to PEPCK promoter (normalized to input) C ... required in the TNFa-mediated inhibition of PEPCK [54] Therefore, in cells preincubated with TNFa prior to insulin, insulin signalling is prevented, resulting in abrogation of this inhibitory effect ... alterations in PEPCK gene expression In this study, we found Foxa2 to be predominantly localized in the nuclei of HepG2 cells incubated with TNFa prior to insulin incubation As reported earlier, insulin...
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Báo cáo khoa học: Glutathione transferases kappa 1 and kappa 2 localize in peroxisomes and mitochondria, respectively, and are involved in lipid metabolism and respiration in Caenorhabditis elegans pot
Ngày tải lên :
16/03/2014, 03:20
... increased during morphogenesis of the embryo Fluorescence was observed as strong punctate staining in intestinal cells and in the epidermis The number and intensity of fluorescent structures increased ... transferase kappa in C elegans E Petit et al using Image J software and then processed using Adobe PhotoshopÒ To stain mitochondria, animals were incubated for 10 with 10 lm of MitoTracker Red (Invitrogen ... RCE1182) In brief, control and RNAi strain cultures were grown for h in LB medium containing 100 mgÆmL)1 ampicillin, and then spread onto NGM agar containing isopropyl thio-b-dgalactoside (1...
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Báo cáo khoa học: The astacin protein family in Caenorhabditis elegans docx
Ngày tải lên :
23/03/2014, 15:21
... and cloned in frame into a pBD95.85 vector (having the S65C mutation and artificial introns to increase the expression of GFP; A Fire Vector Kit, Baltimore, USA) according to standard protocols [23,25] ... occurrence in complement components C1r/C1s, embryonic sea urchin protein Uegf, and BMP-1 [62] These domains may be involved in calcium-binding and protein-protein or enzyme–substrate interactions ... (six-cysteine) motif was observed in several hypothetical C elegans proteins [64,65] but was originally described in metridin, a toxin from sea anemone and is also called ShK toxin domain (SMART...
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Báo cáo khoa học: Identification and localization of glycine in the inner core lipopolysaccharide of Neisseria meningitidis ppt
Ngày tải lên :
23/03/2014, 21:21
... without ions corresponding to glycine containing glycoforms (Table 1), thus confirming that the glycine residue is attached to the core oligosaccharide via an ester linkage In order to confirm and further ... indicated in the inset figures also performed on two other meningococcal strains of clinical origin and the compositions of the glycoforms observed are listed in Table In each case where glycine ... compared to the sharp singlet at 3.56 p.p.m observed for the -CH2- protons of the glycine moiety in meningococcal LPS points to an exocyclic location for the glycine residue in N meningitidis LPS The...
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A pathway that links reproductive status to lifespan in Caenorhabditis elegans pptx
Ngày tải lên :
28/03/2014, 16:20
... germline activates a signaling pathway, what is the initiating event? How is it linked to germline stem cell proliferation? Finally, it is interesting to ask whether this same reproductive signaling ... partially distinct from the pathway that localizes DAF-16 to nuclei when insulin/IGF-1 signaling is inhibited In longlived daf-2 (insulin/IGF-1-receptor) mutants, DAF16/FOXO accumulates in most or ... target-gene expression in response to germline ablation, but (for at least some of the same genes) not in response to inhibition of insulin/IGF-1 signaling, and this is an interesting question Whatever...
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Báo cáo sinh học: "The short coiled-coil domain-containing protein UNC-69 cooperates with UNC-76 to regulate axonal outgrowth and normal presynaptic organization in Caenorhabditis elegans" ppt
Ngày tải lên :
06/08/2014, 18:21
... used to study vesicular transport in neuronal development For example, monomeric kinesin UNC-104/KIF1A, UNC-116/kinesin heavy chain (KHC), kinesin light chain KLC-2, and various cytoplasmic dynein ... cotransformed into yeast cells with vector alone or vectors containing GAD-unc-76␥, GAD-arl-1, GAD-arl-3, or GAD-arfrp Protein-protein interactions were measured as -galactosidase activity by using ONPG ... motor column (LMC) and the medial motor column (MMC) In addition to neural tissues, staining was also present in the dermamyotome (Figure 5k) Blocking the function of SCOCO with in ovo RNA interference...
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