immobilization of glucoamylase on yeast cell surface using an integrative plasmid

Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx

Ngày tải lên : 12/08/2014, 04:21

... method of choice to detect and quantify virus infections in clinical samples, including enteroviruses [26,27] Amplification of highly conserved regions of the enterovirus 5'untranslated region (5'UTR) ... of infection (MOI) of 0.05 TCID50 /cell) or MOI 0.05 TCID50 /cell of crude virus extracts were incubated with cells in suspension on ice for h The attached virus was measured using scintillation ... amount of cells was also investigated A decreasing number of cells were incubated with CVB5 at a concentration of MOI 0.05 TCID50 /cell and significant differences in binding to HeLa in comparison...

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Báo cáo sinh học: "Shipping blood to a central laboratory in multicenter clinical trials: effect of ambient temperature on specimen temperature, and effects of temperature on mononuclear cell yield, viability and immunologic function" potx

Ngày tải lên : 18/06/2014, 19:20

... influence of common variables on T cell subset analysis by monoclonal antibodies Journal of Immunological Methods 1984, 67:243-253 10 Nicholson JKA, Jones BM, Cross GD, McDougal JS: Comparison of T and ... 0.001 Olson et al Journal of Translational Medicine 2011, 9:26 http://www.translational-medicine.com/content/9/1/26 Page of 13 Table Effect of exposure to different incubation conditions on PBMC ... estimated means and 95% confidence intervals of ratios of cell yield to the control sample (RT × 24 h) are shown, both before cryopreservation and upon recovery of cells after cryopreservation P-values...

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báo cáo hóa học:" Effect of borax on immune cell proliferation and sister chromatid exchange in human chromosomes Malinee Pongsavee" docx

Ngày tải lên : 20/06/2014, 00:20

... final concentration of 0.1, 0.15, 0.2, 0.3 and 0.6 mg/ml for 24 hours The value of mean absorbance reflected the anti-proliferation of lymphocyte by borax Mean absorbance conversion can be directly ... with the amount of formazan produced by untreated control cells, the effectiveness of the agent in causing death of cells can be deduced, through the production of a dose-response curve [7] Various ... formazan in the mitochondria of living cells A solubilization solution is added to dissolve the insoluble purple formazan product into a colored solution The absorbance of this colored solution can...

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Báo cáo hóa học: " Quantitative profiling of housekeeping and Epstein-Barr virus gene transcription in Burkitt lymphoma cell lines using an oligonucleotide microarray" docx

Ngày tải lên : 20/06/2014, 01:20

... http://www.virologyj.com/content/3/1/43 Figure induction of lytic EBV infection on Zta and EBV gene transcription Effect of4 Effect of induction of lytic EBV infection on Zta and EBV gene transcription (A) Western ... mechanisms governing the maintenance of EBV latent infection, host cell transformation, and reactivation of lytic infection Thus, a research tool to quantify simultaneous EBV gene transcription ... transcription levels of Zta in IgG cross-linking induced (+) and non-treated (-) Akata cells (B) Transcription of EBV genes was quantified by microarray analysis in Akata cells upon induction of...

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Modeling Of Wire On Tube Heat Exchangers Using Finite Element Method

Ngày tải lên : 19/03/2016, 13:14

... to analyse numerically the performance of a wire -on- tube heat exchanger either under free convection or forced convection conditions The present paper is an attempt in that direction Free convection ... 313 a Coil #5 Location of starting of phase change in tube # Location of starting of sub-cooling in tube # Location of starting of phase change in tube # Location of starting of sub-cooling in ... convection condition on the outside of the tubes normally exists and hence this case is considered for the analysis of wire -on- tube heat 420 G.A Quadir et al / Finite Elements in Analysis and Design...

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Electronic transport properties of single crystal silicon nanowires fabricated using an atomic force microscope

Ngày tải lên : 16/03/2014, 15:15

... fabricated using conventional photolitho- Fig Schematics of the process for silicon nanodevice fabrication involving an AFM lithography step Inset: transmission electron micrograph of a thinned Unibond ... structure of the Si upper layer graphy processes This connection pattern is made of highly doped silicon obtained by phosphorous ion implantation and consists in 100 m2 contact pads connected ... to a local oxidation of the substrate via a ÿeld-enhanced oxidation process by anodization [10] The oxidation reaction occurs only if Utip is chosen higher than a threshold of −2:7 V [11,12]...

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Báo cáo lâm nghiệp: "Automatic determination of fiber orientation on OSB surface using line detection method based on small eigenvalue analysis" doc

Ngày tải lên : 08/08/2014, 00:22

... movement and vibration worked like a filter to cause the small strands to align random Most of the small strands fall and collect on the surface of a conveyer, which forms the undersurface of the ... peak of the distribution RESULTS AND DISCUSSION The original pictures of the top surface and undersurface are shown in Figures 3a and 4a It can be seen that the flake alignment on the top surface ... of angles: mean angle and central angle, which can be used to express the main stream of the flake alignment on the surface of OSB As shown in Figure 7, it is much better to use the central angle...

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Báo cáo y học: "Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers" docx

Ngày tải lên : 09/08/2014, 01:23

... functional quality of the cell populations used for transplantation The methods of FACS analysis and cell sorting offer important approaches for quality control and application of cell populations ... based on the quantification by FACS analysis using triple staining of MPC related cell surface markers In addition, Jones and coworkers [22] recently identified cells with characteristics of MPCs ... human marrow stroma J Cell Biochem 2002, 87:51-57 Guo XM, Wang CY, Wang YH, Duan CM, Zhao Q, Sun DM: Experimental study of the isolation, culture and in chondrogenic differentiation of human bone...

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Báo cáo y học: " Expression of Nef from unintegrated HIV-1 DNA downregulates cell surface CXCR4 and CCR5 on T-lymphocytes" docx

Ngày tải lên : 12/08/2014, 23:23

... origin of replication and so is not maintained upon cell division Therefore, the stability of unintegrated DNA in dividing cells is governed by the rate of cell division [23,24] Insertion of an SV40 ... P, Gluckman J, Canque B: Cell cycle regulation of human immunodeficiency virus type integration in T cells: antagonistic effects of nuclear envelope breakdown and chromatin condensation Virology ... that Nef translation from unintegrated DNA can occur at Page of 10 functionally relevant levels, and leads to reduced cell surface expression of CXCR4 and CCR5 as well as CD4 Additional work to...

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Báo cáo y học: " Antibody microarray analysis of cell surface antigens on CD4+ and CD8+ T cells from HIV+ individuals correlates with disease stages" ppt

Ngày tải lên : 13/08/2014, 05:22

... decreased expression of CD38 on CD8+ T cells is highly correlated with the effectiveness of antiretroviral therapy [4-8] and lack of activation and expression of CD38 and HLA-DR on CD4+ T cells correlates ... simultaneously analyze 135 different cell surface antigens (128 cluster of differentiation antigens plus other surface antigens) on peripheral blood CD4+ and CD8+ T cell subsets from HIV+ and HIV- ... changes in the expression of activation markers were more pronounced in CD8+ T cells, whereas changes in the expression of cell membrane receptors for cytokines and chemokines were more pronounced...

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Báo cáo y học: "Longitudinal microarray analysis of cell surface antigens on peripheral blood mononuclear cells from HIV+ individuals on highly active antiretroviral therapy" doc

Ngày tải lên : 13/08/2014, 06:20

... sustained responders and transient responders Our study not only demonstrated potential associations between modulations of cell surface antigens and activation or restoration of the immune system, ... between modulation of antigen expression and changes in the proportion of cell population expressing the antigen A biological analysis of these markers may also help to clarify their role and may lead ... software on an 8-bit pixel grey scale from 0–255 The dot intensity reflects cell binding density, which depends on both the level of expression of a particular antigen and the proportion of cells...

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Effect of Process Parameters on the Degradation of Polychlorinated Biphenyls in Water Matrix using UV/H2O2

Ngày tải lên : 05/09/2013, 09:08

... solutions The initial concentration of the solution being irradiated was also found to have significant effects on the rate of pollutant degradation as well as the kind and concentration of intermediates ... H2O2 concentration of about 2800 ml (of the 30%H2O2 solution) per liter and initial PCB concentration of about 40 ppm The initial pH of the solution also has a significant effect on the rate of ... study of Wang et al (2000) on the degradation of humic acids cited that, at a fixed concentration of hydrogen peroxide, increasing the humic acid concentration decreases the rate of degradation Wang...

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Prediction of deformation and hygro-thermal stresses distribution in PEM fuel cell vehicle using threedimensional CFD model

Ngày tải lên : 05/09/2013, 14:58

... membrane An operating fuel cell has varying local conditions of temperature, humidity, and power generation (and thereby heat generation) across the active area of the fuel cell in three-dimensions ... electrolyte fuel cell Variations in operating modes and general cell design according to application means that how dominant certain performance degradation and failure mechanisms are also change according ... mass balance is described by the divergence of the mass flux through diffusion and convection Multiple species are considered in the gas phase only, and the species conservation equation in multicomponent,...

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Investigation of thermal effect on exterior wall surface of building material at urban city area

Ngày tải lên : 05/09/2013, 15:28

... can increase the awareness of urban planners, designers and decision makers on the importance of the construction material selection, which is not only for their esthetical aspect but also on ... radiation) of each material was calculated and analyzed (Figure 10) Figure 10 Normalized heat (total heat/solar radiation) using average heat of convection (qconv), heat of radiation (qrad) and ... diurnal and seasonal anthropogenic heating profiles for urban areas Atmos Environ 2004, 38, 2737–2748 [8] Wong N.H., Chena Y., Ongb C.L., Siab A (2005) Investigation of thermal benefits of roof top...

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Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt

Ngày tải lên : 19/02/2014, 05:20

... is present on the surface of a variety of cell lines [42,48,52,55,56] and on the surface of endothelial cells [57,58] To address whether nucleo4674 lin is also present on the surface of developing ... sepharose (lane 2) using polyclonal rabbit anti-nucleolin serum (E) Immunoblot of column eluates from blank sepharose (lane 1) and lactoferrin sepharose (lane 2) using polyclonal rabbit anti-nucleolin ... experiments were carried out using the protein lactoferrin Lactoferrin, an ironbinding protein of the transferrin family, is present in external secretions and the secondary granules of FEBS Journal 273...

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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx

Ngày tải lên : 21/02/2014, 03:20

... target and partners of bFGF (Eur J Biochem 269) 509 Fig Action of bFGF on Glypican-1, syndecan-1 and syndecan-4 mRNAs expression during Sertoli cells development Sertoli cells from 10-, 20- and ... estradiol production, we evaluated if bFGF itself could in¯uence syndecan-1, syndecan-4 and glypican-1 mRNAs expression bFGF effect on glypican-1, syndecan-1 and syndecan-4 mRNAs expression The relative ... not (control) of FSH (100 ngáml)1) and of bFGF increasing concentrations Determination of estradiol production was performed by radioimmunoassay Values are expressed in pg per 106 cells and are...

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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx

Ngày tải lên : 06/03/2014, 22:21

... combinations of single and double putative N-glycosylation site mutants using site-directed mutagenesis The wild-type and mutant expression constructs were transfected into HEK293T cells and the ... compilation ª 2010 FEBS L Bhatt et al N-Glycosylation of HFE A B Fig ER localization of the HFE-triple mutant and interaction of N-glycosylation site mutants with b2M (A) HuTu80 cells transfected ... transfected cells continued to exhibit ERC/punctate localization and regulate transferrin binding, consistent with production and localization of a functional protein Many studies have demonstrated...

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Báo cáo khóa học: Identiﬁcation of residues controlling transport through the yeast aquaglyceroporin Fps1 using a genetic screen ppt

Ngày tải lên : 07/03/2014, 15:20

... full function None of the mutants was more abundant than wild type Fps1, excluding simple expression changes as a cause for the observed gain of function Phenotypic characteristics of mutants obtained ... grow on xylitol, suggesting that in some of the mutants isolated inﬂux may be enhanced to a higher degree than efﬂux Discussion The transmembrane transport of glycerol in yeast is rapidly controlled ... function under these conditions This effect was most pronounced for C-terminal truncations, some of which conferred sensitivity almost like deletion of FPS1 (Fig 1) In addition, mutations at...

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Báo cáo " Study on wave prevention efficiency of submerged breakwater using an advanced mathematical model " pot

Ngày tải lên : 14/03/2014, 15:20

... Fig Variation of reflection, transmission and dissipation coefficient versus water depth at the top of the breakwater For the transmission and dissipation coefficients, the variation is very ... presentation of variation of three quantities, the reflection, transmission and dissipation coefficients, versus the change of the breakwater width It is worthy to note that the dissipation coefficient ...

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