immobilization of beta glucosidase on yeast cell surface
Investigation of thermal effect on exterior wall surface of building material at urban city area
... designers and decision makers on the importance of the construction material selection, which is not only for their esthetical aspect but also on the local climate and energy consumption of buildings ... heat/solar radiation) of each material was calculated and analyzed (Figure 10) Figure 10 Normalized heat (total heat/solar radiation) using average heat of convection (qconv), heat of radiation (qrad) ... areas Atmos Environ 2004, 38, 2737–2748 [8] Wong N.H., Chena Y., Ongb C.L., Siab A (2005) Investigation of thermal benefits of roof top garden in the tropical environment Build Environ 2005, 38,...
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Effect of tunneling current on the growth of silicon islands on si(111) surfaces with a scanning tunneling microscope
... to the surface Experimental We used a UHV STM combined with a scanning reflection electron microscope (SREM ) The condition of the tungsten tip of the STM and the manipulation of the tip on the ... measurements of the nanostructure growth provide data which throw light on the mechanism of nanostructure formation Conclusion The early- and late-stage rates of silicon island growth on Si(111) surfaces ... transfer of silicon atoms from the STM tip by re-evaporation of the silicon that was accumulated on the STM tip at the initial stages of the tip– sample interaction very likely occurs in the growth of...
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báo cáo khoa học: " In vitro behaviour of endothelial cells on a titanium surface" doc
... degree of expression of adhesion molecules on the surface of human ECs depends on the response of the cells against the implanted material [11] PECAM-1 is a cell- cell junction molecule that establishes ... Upon one-day Ti /cell contact the expression of fibronectin was predominantly cytoplasmatic and stronger than on the control surface (Figure 5A and 5D) After and 14 days we observed that fibronectin ... both surfaces with a more dense mesh on the control surfaces The cells produced and released fibronectin in Figure after the studied periods Attachment of HUVECs on titanium and on control surfaces...
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First principles study of benzene adsorption on transition metal surfaces
... energy ΔHads Heat of Adsorption ΔΦ Change in workfunction of metal surface upon adsorption Φ Workfunction of metal surface θ Surface coverage in computational methods θexp Surface coverage in ... center of nuclei, contributing to the overestimation of bond length; however, the latter contributes to underestimation of bond length arising from correlation with electrons in long-range on molecular ... interaction will be briefly presented Finally, electronic interaction of benzene adsorption on transition metal surface will be explained 1.4.1 Surface- adsorbate interaction Chemical bonding constructed...
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Báo cáo khoa học: Investigation of the effects of sulfonylurea exposure on pancreatic beta cell metabolism pptx
... the effects of prolonged exposure to sulfonylurea drugs on beta cell metabolism using a combination of different NMR techniques, and utilizing the well-characterized clonal glucose-responsive pancreatic ... stimulation of exocytosis by sulfonylureas in pancreatic beta cells Science 271, 813–815 Tian YA, Johnson G & Ashcroft SJ (1998) Sulfonylureas enhance exocytosis from pancreatic beta- cells by ... perspectives on the actions of sulphonylureas and hyperglycaemic sulphonamides on the pancreatic betacell Diabete Metab 20, 157–162 Kamp F, Kizilbash N, Corkey BE, Berggren PO & Hamilton JA (2003) Sulfonylureas...
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Báo cáo y học: " Expression of Nef from unintegrated HIV-1 DNA downregulates cell surface CXCR4 and CCR5 on T-lymphocytes" docx
... the context of replication competent viruses [34-36] In order to confirm that the Rev-CEM cell line was suitable for the study of Nef-mediated downregulation of cell surface receptors from cells ... http://www.retrovirology.com/content/7/1/44 lation of CCR5 seen with integrated infections of TZM/bl cells [36] Studies with 293-Affinofile cells, a cell line that is quantitatively inducible for both CD4 and CCR5 cell surface ... typically 7% of the total population studied Infected cells were measured by flow cytometry for cell surface expression of CD4, CXCR4 and CCR5 A pattern of downregulation, similar to that of integrating...
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Báo cáo y học: " Antibody microarray analysis of cell surface antigens on CD4+ and CD8+ T cells from HIV+ individuals correlates with disease stages" ppt
... decreased expression of CD38 on CD8+ T cells is highly correlated with the effectiveness of antiretroviral therapy [4-8] and lack of activation and expression of CD38 and HLA-DR on CD4+ T cells correlates ... demonstrate the conservation of unique cell surface antigen mosaics in cryopreserved PBMCs from HIV+ individuals Here, we have used an antibody microarray constructed on the surface of a nitrocellulose ... CD8+/CD11b+ T cells during progression of HIV infection in asymptomatic patients [38], we are not aware of any previous reports of modulations in CD11b expression on CD4+ T cells of HIV+ patients...
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Báo cáo y học: "Longitudinal microarray analysis of cell surface antigens on peripheral blood mononuclear cells from HIV+ individuals on highly active antiretroviral therapy" doc
... sustained responders and transient responders Our study not only demonstrated potential associations between modulations of cell surface antigens and activation or restoration of the immune system, ... HAART, cell surface expression of adhesion molecules and levels of circulating adhesion molecules requires further investigation Three cell surface antigens were associated with cell activation (CD9, ... expression over time was consistent with a previous study, which showed lack of control of T cell apoptosis under HAART [23] CD2 mediates both cell- to -cell adhesion and T cell activation; also...
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Báo cáo y học: "HLA-DR regulation and the influence of GM-CSF on transcription, surface expression and shedding
... glycosylated cell surface transmembrane protein expressed on antigen-presenting cells and constitutively expressed on monocytes Expression of HLA-DR by monocytes is essential for the presentation of peptides ... conjugate, clone L243 (Becton Dickinson, UK) and with a mouse IgG anti-human CD14 R-phycoerythrin conjugate (R-PE), clone M5E2, (Becton Dickinson, UK) to identify monocytes Briefly 100 µl of cells ... surface A comparison to the surface value allowed an estimate of intracellular versus extracellular expression of HLA-DR Healthy controls (n=6) had lower expression of intracellular HLA-DR than...
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A CFD analysis on the effect of ambient conditions on the hygro-thermal stresses distribution in a planar ambient airbreathing PEM fuel cell
... (corresponding to the assembly conditions) [11, 12, 28] In addition, a constant pressure of (1 MPa) is applied on the upper surface of cathode, corresponding to a case where the fuel cell is ... Navier-Stokes equations, i.e the continuity equation, the mass conservation equation for each phase yields the volume fraction (r ) and along with the momentum equations the pressure distribution inside ... (b) longitudinal cross section of 3 -cell fuel cell stack ISSN 2076-2895 (Print), ISSN 2076-2909 (Online) ©2011 International Energy & Environment Foundation All rights reserved International...
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Tài liệu Báo cáo " Effecting of medium composition on biomass and ginsenoside production in cell suspension culture of Panax vietnamensis Ha et Grushv " doc
... medium containing 3% sucrose Effects of application sole nitrate (NO3-) and in combination with ammonium (NH4+) on production of ginseng saponin and polysaccharides by suspension cultures of Panax ... nitrogen concentration of 40 mM [12] In the simultaneous production of ginseng saponin and polysaccharide by suspension cultures of P ginseng, [4] reported that production of ginseng saponin was ... Mean separation by Duncan’s multiple range test at p ≤ 0.05 Effect of different sucrose concentrations on cell growth and ginsenoside production The effect of initial sucrose concentration (0, 20,...
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Tài liệu Báo cáo khoa học: Cell surface nucleolin on developing muscle is a potential ligand for the axonal receptor protein tyrosine phosphatase-r ppt
... is present on the surface of a variety of cell lines [42,48,52,55,56] and on the surface of endothelial cells [57,58] To address whether nucleo4674 lin is also present on the surface of developing ... parent ion tolerance of ± m ⁄ z, a peptide ion tolerance of ± m ⁄ z, one missed cleavage, fixed carbamidomethylation of cysteines and variable oxidation of methionines were specified Purification of ... well as on the surface of nonmyosinexpressing cells Some of the punctate localization also occurred at the cell cell interface between these cells and myotubes (arrowheads in Fig 6D) It is of interest...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... The DNA content of the cell layer at the end of incubation was quanti®ed by the method of West et al [31] After solubilization of the cell layer in M NaOH and subsequent neutralization by M KH2PO4, ... observed (Table 3) In conclusion, bFGF requires cell surface HSPG for the inhibition of steroidogenesis in developing Sertoli cells Cell surface HSPG are bFGF targets during Sertoli cell postnatal development ... Sertoli cells Sertoli cells were incubated for 24 h with testosterone substrate (200 ngáml)1) in the presence or not (control) of FSH (100 ngáml)1) and of bFGF increasing concentrations Determination...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... FEBS 3221 N-Glycosylation of HFE L Bhatt et al A B C Fig Inhibition of N-glycosylation influences patterns of HFE intracellular localization HuTu80 cells were transfected with constructs expressing ... function has not previously been studied In this article, we map the sites of HFE N-glycosylation and examine the importance of glycosylation on parameters of protein localization and function Results ... treatment of HFE-WT-expressing cells results in a reticular localization pattern To definitively establish the importance of N-glycosylation on HFE localization in HuTu80 cells, we transfected cells...
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Báo cáo Y học: Matrilysin (matrix metalloprotease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces docx
... processes, including pericellular proteolysis, cell cell or cell ECM adhesion, and regulation of membrane architecture [13–16] One of the important functions of extracellular annexin II is its ... the existence of annexin II on cell surfaces of WiDr cells (data not shown) To examine whether matrilysin cleaves annexin II on cell surfaces, three kinds of human colon cancer cell lines (CaR-1, ... interaction of annexin II with the cell surface receptors Binding of tPA to surfaces of matrilysin-treated cells It has been shown that tPA binds to annexin II on the surfaces of human endothelial cells...
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Báo cáo khoa học: N-Terminal segment of potato virus X coat protein subunits is glycosylated and mediates formation of a bound water shell on the virion surface docx
... cm)1 region corresponds to superposition of several absorption peaks Here a complex band of valent water OH-bond vibrations is overlapped with peaks of valent OH-bond and NH-bond vibrations of PVX ... 4000 cm)1 region Discussion The role of CP glycosylation in plant virus life cycles remains unclear, although data on the effects of glycosylation on the conformation and dynamics of O-linked glycoproteins ... glycosylation of potato virus X protein (Eur J Biochem 271) 3141 Fig Stages of glycosylation site identification (A) Initial part of RP-HPLC profile of PVX preparation after modification of the trypsin...
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Báo cáo " Effects of macro elements on biomass and ginsenoside production in cell suspension culture of Ngoc Linh ginseng " docx
... Concentration of macro element NH4NO3 KNO3 Determination and analyses Extraction and determination of ginsenoside production were determined as reported previously [8,9] MgSO4 Determination of ... level of secondary metabolism [10,11] demonstrated the effect of phosphate limitations on the accumulation of cinnamoyl putrescines in tobacco cultures, while [12] concluded that the lack of phosphate ... ginseng cell growth and ginsenoside production required higher concentrations of KNO3, MgSO4, and CaCl2 than those normally used in culture media In contrast, however, at low concentration of NH4NO3...
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Đề tài " Curve shortening and the topology of closed geodesics on surfaces " potx
... 2ε0 of the intervals on which one can construct local continuations, so that a finite number of such local continuations will take one from θ = to θ = We will therefore now describe the construction ... -intersections This was observed by Poincar´ [33] We include a proof for completeness’ e sake Proof Intersections of α and γ are of two types Each zero of u(x) corresponds to an intersection of α and ... intersections of α and γ To count self-intersections one must count the intersections of the graph of u(x) = sin(2π p x) wrapped up on the cylinder Γ = (R/Z) × R, i.e the q intersections of the graphs of...
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Báo cáo khoa học: Training of yeast cell dynamics ppt
... 0.8 0.6 Fig Intracellular response of yeast cells to extracellular dynamics The NADH fluorescence (solid line) was measured in a suspension of intact yeast cells Cells were grown on glucose, harvested ... Fig Effect of low continuous and low pulsatile glucose concentrations on the intracellular dynamics of yeast The amplitude of the intracellular NADH oscillation was determined for cells that ... previous studies on glycolytic oscillations there was an aspect of adaptation Alterations in the make-up of the yeast cells made them prone to limit cycle oscillations [6,7] For the cells to engage...
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Báo cáo khoa học: On the mechanisms of glycolytic oscillations in yeast potx
... by means of sensitivity analysis Cell synchronization Frequency modulation is of primary importance for the synchronization of the glycolytic oscillations among the individual yeast cells [30,4244] ... of the cell in terms of all relevant metabolite concentrations Despite the high dimension of concentration space, we show below that, in the specic case of glycolytic oscillations in intact yeast ... reaction is due to the on off nature of the oscillations; it is the regulation of the HK-PFK reaction by ATP that is important for the occurrence of oscillations, not its Vmax This notion can...
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