ilit with dendritic cells
Báo cáo y học: " Eradication of intractable malignant ascites by abdominocentesis, reinfusion of concentrated ascites, and adoptive immunotherapy with dendritic cells and activated killer cells in a patient with recurrent lung cancer: a case report" pps
... of intrathoracic immunotherapy (1.82 × 1010 cells) and 12 of intravenous immunotherapy (5.6 × 1010 cells) with dendritic cells and activated killer cells (DC+AK) obtained from long-term tissue ... was added When dendritic cells and activated killer cells were released from the lymph node tissue, cells were harvested and tissue culture continued until the release of new cells ceased (dormant ... washed and counted The cells were suspended at × 107 cells/ ml In sterile glass tubes, 0.2 ml samples of labeled tumor cells (2 × 104) were mixed with serial dilutions of effector cells (2 × 106 to...
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Báo cáo hóa học: "Multicenter phase II study of matured dendritic cells pulsed with melanoma cell line lysates in patients with advanced melanoma" potx
... total CD8+ cells Detection of Melan A-specific T Cells with MHC tetramers When sufficient cells from HLA-A*0201 positive subjects were available, T cells (106 cells) were stained with Melan A/MART-1 ... FACSCalibur flow cytometer Anti-TAA specific CD8+ cells were defined as cells producing IFN-g (with or without CD107 expression) after stimulation with TAApeptide pools The CD8+ T cell population ... stimulation Cells were then stained with anti-CD8-PE and anti-CD3-PerCP antibodies, fixed and permeabilized with the Intrastain kit, and stained for intracellular IFN-a with an anti-IFN-g-APC antibody Cells...
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Báo cáo y học: " Dysfunctional interferon-α production by peripheral plasmacytoid dendritic cells upon Toll-like receptor-9 stimulation in patients with systemic lupus erythematosus" doc
... B cells, T cells, and dendritic cells IFN-α is mainly produced in plasmacytoid dendritic cells (pDCs); they were originally termed natural IFN-α producing cells [10-12] pDCs are key effector cells ... Lahoud M, Shortman K: Production of interferons by dendritic cells, plasmacytoid cells, natural killer cells, and interferonproducing killer dendritic cells Blood 2007, 109:1165-1173 34 Vallin H, Blomberg ... tolerance, in which cells exhibit no response to subsequent challenges with the same TLR stimulus, has been reported in epithelial cells, neutrophils, macrophages, and conventional dendritic cells [2528]...
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Báo cáo y học: " Myeloid dendritic cells correlate with clinical response whereas plasmacytoid dendritic cells impact autoantibody development in rheumatoid arthritis patients treated with infliximab" pdf
... lymphocytes PBMCs were cultured with influenza virus with or without infliximab After 15 days, we measured the proportion of CD19+CD20-CD38high+ plasma cells PBMCs cultured with influenza virus + infliximab ... plasmacytoid dendritic cells in synovial fluid and tissue of patients with chronic inflammatory arthritis J Immunol 2004, 173:2815-2824 29 Summers KL, Daniel PB, O'Donnell JL, Hart DN: Dendritic cells ... percentage of CD20neg/CD38high cells Synovial fluid was obtained at the initial time point from patients with RA (n = 9) and from patients with osteoarthritis (n = 11), with knee effusions This synovial...
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báo cáo khoa học: "Ganoderma lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function" docx
... DCs derived with GL-PS We detected low amount of IL-12 production from THP-1 cells, THP-1 DCs with GM-CSF/IL-4, THP-1 with GL-PS and GL-PS THP-1 DCs (Fig 5A) Though THP-1 DCs with or without GL-PS ... with GL-PS plus GM-CSF/IL-4 yielded atypical large adherent and elongated cells with multiple cytoplasmic spikes (white arrow) comparing to the round floating THP-1 cells and typical Mo-DCs with ... cycle analysis with PI staining The THP1 (left panel) and U937 cells (right panel) were treated with or without 100 μg/mL GL-PS for three days The cell cycle analysis was then analyzed with PI staining...
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Báo cáo y học: "Differential induction of inflammatory cytokines by dendritic cells treated with novel TLR-agonist and cytokine based cocktails: targeting dendritic cells in autoimmunity" potx
... untreated cells Pre-treatment of DCs with dexamethasone was able to suppress proliferation for all cocktails and LPS Cocktail treated DC with ability to induce IFNg producing T -cells The ability ... Differential induction of inflammatory cytokines by dendritic cells treated with novel TLRagonist and cytokine based cocktails: targeting dendritic cells in autoimmunity Journal of Inflammation 2010 ... bars indicate (SD) values not indicate if the T -cells has differentiated into Th1, Th2 or Th17 T -cells when the DCs are cocultured with CD4+ T cells In order to evaluate whether the cocktails...
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Báo cáo y học: " Inhibitor of IκB kinase activity, BAY 11-7082, interferes with interferon regulatory factor 7 nuclear translocation and type I interferon production by plasmacytoid dendritic cells" ppsx
... stimulated with CpG-2216 with autologous 20% serum in flat-bottomed 48-well plates at 106 cells in 500 μl of medium per well Alternatively, healthy PBMCs were stimulated with 20% lupus serum with or without ... Dead cells were excluded on the basis of side- and forward-scatter characteristics In the viability assay, cells were washed with phosphate-buffered saline(PBS) containing mM EDTA, and viable cells ... BDCA: blood dendritic cell antigen; DC: dendritic cells; IFN: interferon; IKK: IκB kinase; IRF7: IFN regulatory factor 7; PBMC: peripheral blood mononuclear cells; pDC: plasmacytoid dendritic cell;...
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Báo cáo y học: "Abnormalities in circulating plasmacytoid dendritic cells in patients with systemic lupus erythematosus" pps
... cultured with or without apoptotic cells; (2) Antigen loaded or unloaded pDCs were then co-cultured with autologous or allogenous T cells The same control T cells were used as responder cells in ... annexin V and propidium iodide (PI) staining (double positive cells) Interaction of dendritic cells with apoptotic cells pDCs were incubated with apoptotic PMNs for four hours pDCs that had captured ... of patients with systemic lupus erythematosus proliferate mononuclear cells in vitro J Rheumatol 1999, 26:294-301 Tzeng TC, Suen JL, Chiang BL: Dendritic cells pulsed with apoptotic cells activate...
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Báo cáo y học: "TLR2 and TLR4 triggering exerts contrasting effects with regard to HIV-1 infection of human dendritic cells and subsequent virus transfer to CD4+ T cells" docx
... V, Delgado E, Steinman RM: Virus replication begins in dendritic cells during the transmission of HIV-1 from mature dendritic cells to T cells Curr Biol 1999, 9:21-29 Jiang W, Lederman MM, Hunt ... T cells by mature dendritic cells PLoS Pathog 2008, 4:e1000134 Wang JH, Wells C, Wu L: Macropinocytosis and cytoskeleton contribute to dendritic cell-mediated HIV-1 transmission to CD4+ T cells ... stimulated for hours with the listed TLR agonists Thereafter, cells were washed twice and pulsed with NL4-3/Balenv IM-MDDCs were either left untreated (left panel) or treated with EFV (right panel)...
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Báo cáo y học: " HIV interactions with monocytes and dendritic cells: viral latency and reservoirs" pptx
... trans-infection of T cells by mature dendritic cells PLoS Pathog 2008, 4:e1000134 Cavrois M, Neidleman J, Kreisberg JF, Greene WC: In vitro derived dendritic cells trans-infect CD4 T cells primarily with surface-bound ... plasmacytoid dendritic cells PLoS ONE 2007, 2:e458 Groot F, van Capel TM, Kapsenberg ML, Berkhout B, de Jong EC: Opposing roles of blood myeloid and plasmacytoid dendritic cells in HIV-1 infection of T cells: ... of CD1a-sorted myeloid dendritic cells J Virol 2005, 79:602-608 Janas AM, Dong C, Wang JH, Wu L: Productive infection of human immunodeficiency virus type in dendritic cells requires fusion-mediated...
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Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc
... FPGN-containing peptides facilitate the uptake of adenovirus into CD40-expressing cells To test the ability of CD40-binding peptides in facilitating adenovirus entry into CD40-expressing cells, we used a ... GFP-expressing cells for both immature and mature murine Ó FEBS 2003 Fig Adenovirus complexed with FPGN2-KBP preferentially transduced SKBR3 cells transiently transfected with murine CD40 SKBR3 cells ... B cells DC, LCL, L10A, Blasts: these labels refer, respectively, to day human DC, EBV-immortalized human B cells, murine B lymphoma L10A cells and murine LPS blasts Cells (105) were infected with...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... immature dendritic cells that were used as control (100%) with those of dendritic cells made mature by stimulation with LPS and Der p allergen (Fig 1) We found that in mature dendritic cells the ... human immature dendritic cells and in rat Ó FEBS 2002 The endocannabinoid system in human dendritic cells (Eur J Biochem 269) 3775 Fig FAAH, CB1 and CB2 mRNA expression in dendritic cells (A) Expression ... and in dendritic cells (lane 4) lysates when the CB1 antibody was preabsorbed with its corresponding homologous peptide (B) Rat spleen (lane 1) and dendritic cells (lane 2) lysates reacted with...
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Báo cáo khoa học: Differential post-translational modification of CD63 molecules during maturation of human dendritic cells potx
... Biochem 270) Fig CD63 isoforms in immature and mature dendritic cells (A) Immature dendritic cells, mature dendritic cells (DC) and Mel JuSo cells incubated for 48 h in the absence or presence ... immature and mature dendritic cells Poly N-acetyl lactosaminoglycans on CD63 and LAMP molecules during maturation of dendritic cells (A,B): Dendritic cells were incubated with LPS for the times ... immature and mature dendritic cells, CD63 as well as LAMP were largely present within pool I, indicating that before and after maturation of dendritic cells, CD63 remained localized within MHC class...
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Optimal Design of a Hybrid Electric Car with Solar Cells pptx
... season, can be obtained with an intermittent use of the vehicle at limited average power, compatible with typical use in urban conditions during working days The fuel saving with respect to conventional ... impressive, considering that a HEV can save about 40% with respect to actual cars This result has been obtained with commercial PV panels and with realistic data and assumptions on the achievable ... sides and rear at almost vertical positions (the practical feasibility of this solution is questionable, also due to the limited reliability of panels in case of lateral impacts) APV ,V ,MAX = (2l...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... fusion cells could prime naive T cells to differentiate into CTL with lytic activity against the HCC cells (Figure 3A and 3B) After hr coculture of the HCC cells with healthy donor's T cells stimulated ... addition of effector cells Cells were washed, fixed with Cytofix/Cytoperm Solution (BD Pharmingen) and then washed with Perm/Wash Buffer (BD Pharmingen) Cells were incubated with FITC-conjugated ... and NK-sensitive K562 cells were labeled with the red fluorescence dye PKH-26 (Sigma, St Louis, MO) After washing with PBS, PKH-26-labeled target cells were cultured with T cells for h at 37°C...
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báo cáo hóa học:" Protective CD8+ T-cell responses to cytomegalovirus driven by rAAV/GFP/IE1 loading of dendritic cells" pdf
... PBMCs and K562 cells did not express IE1 (data not shown) T -cells incubated with AAV/IE1loaded DCs were able to kill the IE1-positive autologous target cells These data are consistent with a strong ... E7 antigen gene transduction into dendritic cells Eur J Immunol 2002, 32:30-38 Young JW, Inaba K: Dendritic cells as adjuvants for class I major histocompatibility complex-restricted antitumor ... antibodies, at a concentration of 25 μg/mL, were pre-incubated with the target cells for 30 minutes before addition of the stimulated T -cells K562 cells were used as targets to observe natural killer (NK)...
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báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
... incubated with allogeneic T cells stained with CFSE (F) Total non-transduced (NT) and rAAV2/1, rAAV2/2 or rAAV2/5 transduced unsorted GEN2.2 were incubated with allogeneic T -cells stained with CFSE ... LV with a PGK promoter pseudotyped with either VSVG and RD114 envelopes at a MOI of 18 or with the GaLV envelope at a MOI of (B) CD34+ progenitors were transduced with VSVG pseudotyped-LV with ... transduced by LV with a PGK promoter pseudotyped with either VSVG and RD114 envelopes at a MOI of 18 or with the GaLV envelope at a MOI of (B) GEN2.2 were transduced with VSVG pseudotyped-LV with a PGK,...
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báo cáo hóa học:" Short-term cultured, interleukin-15 differentiated dendritic cells have potent immunostimulatory properties" ppt
... used in the present study Dendritic cell differentiation IL-4 differentiated dendritic cells (IL-4 DCs) GM-CSF 800 IU/mL IL-4 20 ng/mL IL-15 differentiated dendritic cells (IL-15 DCs) GM-CSF 800 ... monocyte-derived dendritic cells Immunol Cell Biol 2009, 87:606-614 Jacobs B, Wuttke M, Papewalis C, Seissler J, Schott M: Dendritic cell subtypes and in vitro generation of dendritic cells Horm Metab ... Figure mRNA transfectability of mature IL-15 DCs mRNA transfectability of mature IL-15 DCs Monocytes were cultured for days with GM-CSF + IL-15, followed by a 24-hr incubation with a TLR7/8 agonist-based...
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Báo cáo hóa học: " Molecular signatures of maturing dendritic cells: implications for testing the quality of dendritic cell therapies" pptx
... monocyte-derived dendritic cells Proc Natl Acad Sci USA 2009 20 Martinez-Nunez RT, Louafi F, Friedmann PS, Sanchez-Elsner T: MicroRNA155 modulates pathogen binding ability of Dendritic Cells by downregulation ... signature in dendritic cell vaccines for cancer immunotherapy Hum Immunol 2009 22 Muthuswamy R, Urban J, Lee JJ, Reinhart TA, Bartlett D, Kalinski P: Ability of mature dendritic cells to interact with ... expressed in human activated and mature dendritic cells through serial analysis of gene expression Blood 2000, 96:2206-2214 Young JW, Merad M, Hart DN: Dendritic cells in transplantation and immune-based...
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Báo cáo hóa học: " Three-day dendritic cells for vaccine development: Antigen uptake, processing and presentation" pot
... IFN-g release from T cells after stimulation with standard mDC loaded with long peptide was previously noted to be much higher than stimulation with standard mDC loaded with short Page 11 of ... interleukin 10-producing, nonproliferating CD4(+) T cells with regulatory properties by repetitive stimulation with allogeneic immature human dendritic cells J Exp Med 2000, 192:1213-1222 Bürdek et ... light microscopy (C) DC were incubated without or with 10 μg/ml FITC-dextran at 37°C or at 4°C for hour The cells were then washed three times in ice-cold PBS with 1% FCS The uptake of FITC-dextran...
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