ikk iκb nf κb signals and nf κb nuclear translocation in lps activated macrophages
Báo cáo y học: "Effect of a small molecule inhibitor of nuclear factor-κB nuclear translocation in a murine model of arthritis and cultured human synovial cells" pps
... These include a proteasome inhibitor PS-341 [14], and IκB kinase inhibitors BMS-345541 [15] and SPC 839 [16], which improved clinical and pathologic findings in murine arthritis Another NF- κB inhibitor ... DHMEQ inhibits TNF-α-induced nuclear translocation of NF- κB, and does not inhibit phosphorylation and degradation of IκB, or a c-Jun N-terminal kinase (JNK) and a caspase-activating pathway in Jurkat ... degradation of IκB by the proteasome and passage of NF- κB into the nucleus [2] NF- κB regulates the expression of many genes that are involved in inflammatory responses, including TNF-α, IL-1β,...
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Báo cáo y học: "Strong inhibition of TNF-α production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657, a p38 mitogen-activated protein kinase (MAPK) inhibitor" ppt
... variety of stimuli both in primary cells and in cell lines [9] In the RA synovium, p38 MAPK is predominantly activated in endothelial cells and in the lining layer [10] Inhibition of p38 MAPK ... induced by LPS stimulation, and no inhibition by p38 MAPK inhibitor was observed This latter finding is in accordance with the study by Lai and co-workers showing that LPS induction of MMP9 in ... of the stress -activated protein kinases, extracellular signal-regulated kinase, c-JUN N-terminal kinase, and p38 mitogen -activated protein kinase, in synovial tissue and cells in rheumatoid arthritis...
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Báo cáo y học: " Regulation of IκBα expression involves both NF-κB and the MAP kinase signaling pathways." ppt
... bortezomib and several MAP kinase inhibitors on LPS- induced IκB expression The results that follow suggest that, in addition to NF- κB, the MAP kinase signaling pathway is involved in controlling IκB ... to affect LPS- induced luciferase expression Bortezomib inhibited LPS- induced IκB expression Using the IκB -luc model, we tested the effect of bortezomib on LPS- induced IκB expression in vivo ... the NF- κB signaling pathway by IκB [6] By reflecting NF- κB transcriptional activity, the luciferase signal in the IκB -luc mouse provides a convenient approach for in vivo monitoring of NF- κB...
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Báo cáo y học: " Functional inhibition of NF-κB signal transduction in αvβ3 integrin expressing endothelial cells by using RGD-PEG-modified adenovirus with a mutant IκB gene" ppsx
... TNF-α activated HUVECs infected with AddnIκB (solid line); and TNF-α activated HUVECs infected with AdLacZ control virus (dotted line) FITC, fluoroscein isothiocyanate MIF, mean fluorescence intensity ... determined by flow cytometry Minus and plus signs and denotes resting, non-infected, and TNF-α activated, non-infected HUVECs, respectively All other histograms represent the responses of dnIκB ... endothelial NF B [9-13] A dominant negative form of IκB (dnIκB) that contains serineto-alanine mutations at amino acids 32 and 36 blocks endogenous IκB phosphorylation and subsequent proteosomemediated...
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Báo cáo y học: " Mitogen-activated protein kinases and NFκB are involved in SP-A-enhanced responses of macrophages to " pdf
... increased activation of intra-macrophage signalling pathways involving MAP kinases and NF B We have examined the role of both the MAPK pathway and NF B activation in BCG killing and nitric oxide production ... plays in enhancing signaling in macrophages infected with BCG Specifically we have examined the effect of SP-A on activation of the MAP kinases ERK1/2 and the transcription factor NF B In initial ... reported that SP-A interaction with macrophages and macrophage cell lines resulted in production of reactive oxygen and nitrogen species and inflammatory cytokines, and activated NF B [60-64] Vazquez...
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Báo cáo y học: "Basal shuttle of NF-κB/IκBα in resting T lymphocytes regulates HIV-1 LTR dependent expression" ppsx
... activation of genes involved in cell survival In resting HIV-infected T cells, the balance between free NF- κB and NF- κB /IκB complexes in the nucleus could directly participate in the maintenance of ... NF- κB /IκB complexes in CD4+ T cells and IκB pool dependence on de novo protein synthesis Analysis of nuclear NF- κB /IκB complexes in CD4+ T cells and IκB pool dependence on de novo protein synthesis ... existence of transdominant mutants of IκB that block NF- κB induction and inhibit de novo HIV infection in T cells by interfering with viral transcription [20,33] Besides, control of IκB by other cellular...
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Báo cáo y học: "Inhibition of HIV-1 replication in primary human monocytes by the IκB-αS32/36A repressor of NF-κB" docx
... Reduced NF- κB DNA binding activity in cells transfected with pNL(AD8 )IκB- αS32/36A Figure Reduced NF- κB DNA binding activity in cells transfected with pNL(AD8 )IκB- αS32/36A Panel A shows the NF- κB binding ... 1structure and expression of recombinant pNL(AD8 )IκB- αS32/36A and pNL(AD8 )IκB- antisense molecular genomes Genome Genome structure and expression of recombinant pNL(AD8 )IκB- αS32/36A and pNL(AD8 )IκB- antisense ... pNL(AD8 )IκB- αS32/36A or pNL(AD8 )IκB- antisense, and the respective nuclear extracts were evaluated for NF- κB (Fig 2A) and Sp1 DNA binding activity (Fig 2B) A significant reduction in NF- κB DNA binding activity was...
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Báo cáo Y học: Properties of group I allergens from grass pollen and their relation to cathepsin B, a member of the C1 family of cysteine proteinases pdf
... 83 and 139 when compared with the proteinases of G lamblia and G gallus All Cys and Trp residues are absolutely conserved in a-expansins and b-expansins as well as in the C1 cysteine proteinases ... respective N-termini was determined by N-terminal sequencing, resulting in the sequence Y-I-P-K-V, confirming the correct processing of the yeast (a-mating factor) signal sequence for protein export ... rPhl p Q during expression and purification, and rPhl p N-containing supernatant Fig Comparison of the expression and enzymatic activity of recombinant Phl p N and Q Sizes in kDa are indicated (A)...
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Báo cáo Y học: Chemical structure and immunoreactivity of the lipopolysaccharide of the deep rough mutant I-69 Rd–/b+ of Haemophilus influenzae docx
... monofunctional Kdo transferase and a phosphokinase of H in uenzae in E coli resulting in LPS which contained exclusively Kdo4P [7] As the LPS obtained from this recombinant strain was deacylated by the ... were washed in NaCl/Pi, blocked for h with blocking buffer (2.5% casein in NaCl/Pi) and then incubated for h with mAb diluted in blocking buffer (50 lL) Plates were washed in NaCl/Pi and incubated ... no binding was observed with antigens containing Kdo-5P instead of Kdo-4P or with antigens containing nonphosphorylated Kdo Fig Binding curves of mAbs S42-16 (A and B), S42-21 (C and D), and S42-10-8...
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Báo cáo y học: "κ The TRAF6-NFκB signaling pathway in autoimmunity: not just inflammation" ppsx
... in blue IL, interleukin; IRAK, IL-1 receptorassociated kinase; MAPK, mitogen -activated protein kinase; mTEC, medullary thymic epithelial cell; NF B, nuclear factor κB; NIK, NF B inhibitory kinase; ... TNF, tumor necrosis factor; TRAF6, TNF receptor associated factor p100 to p52 as a result of phosphorylation by NF B inhibitory kinase (NIK), in both cases allowing translocation of released NF B ... T cells in the periphery, and multi-organ inflammation [19-21] κ TRAF6 and NF B deficient mice define elements of the central tolerance pathway TRAF6 acts like a junction, transducing signals...
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Báo cáo y học: " Inhibitor of IκB kinase activity, BAY 11-7082, interferes with interferon regulatory factor 7 nuclear translocation and type I interferon production by plasmacytoid dendritic cells" ppsx
... IFNα and TNF-α in pDCs is that BAY11 targets IKK in the inhibition of IFN-α and IKK in the inhibition of TNF-α at the downstream of TLR7/9-MyD88 The other two IKK- related kinases, TANK-binding ... IKK and IKK in activating NF- κB in the absence of IKK [40] Given the function of BAY11 as an inhibitor of IKK activity [18,25], a more plausible explanation for its inhibitory activities in ... for the induction of proinflammatory cytokines and type I IFNs, respectively [2,5] Whereas IRF7 phosphorylation and nuclear translocation depend on IKK , NF- κB activation needs IKK IKK homodimer...
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Báo cáo y học: "Mechanical signals control SOX-9, VEGF, and c-Myc expression and cell proliferation during inflammation via integrin-linked kinase, B-Raf, and ERK1/2-dependent signaling in articular chondrocytes" pot
... integrin-linked kinase in mediating tubular epithelial to mesenchymal transition and renal interstitial fibrogenesis J Clin Invest 2003, 112:503-516 Wu C, Dedhar S: Integrin-linked kinase (ILK) and ... [12,13] Integrin-linked kinase (ILK), located in the cytoplasmic domain of integrins, plays a key role in transmitting mechanical signals to the intracellular compartment [13-15] Within the cells, ... al., Mechanical signals control SOX-9, VEGF, and c-Myc expression and cell proliferation during inflammation via integrinlinked kinase, B-Raf, and ERK1/2-dependent signaling in articular chondrocytes...
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Báo cáo y học: "Tumor necrosis factor-alpha promotes survival in methotrexate-exposed macrophages by an NF-B-dependent pathwa" pptx
... effect of TNF-a, we exploited the species specificity of TNF-a action Murine TNF-a (mTNF-a) activates both TNF-R1 and TNF-R2 on murine macrophages, while human TNF-a (hTNF-a) activates TNF-R1 only, ... TRAF2 (TNFR-Associated Factor 2), RIP (Receptor-Interacting Protein) c-Src and Jak2 These include the NF- B pathway, Phosphatidylinositol3-Kinase/Protein Kinase B (PI3K/AKT) and MitogenActivated ... the inflamed synovium, including IL-1, RANKL, GM-CSF, VEGF, PDGF and leukotrienes [37] can promote NF- B signaling in responsive cells and may contribute, with TNF-a, to NF- B activation in macrophages...
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Báo cáo y học: "Participation of the PI-3K/Akt-NF-κB signaling pathways in hypoxia-induced mitogenic factor-stimulated Flk-1 " pot
... through NF- κB route Transfection of dominant negative mutants of IKK kinases, IKK (K44A) and IKK (K44A), and an IκB super-repressor, I κB (S32A/S36A), abolished HIMF-induced NF- κB activity and ... from NF- κB [23] In the present study, we found that HIMF administration induced phosphorylation of IKK and IκB Moreover, transfection of the dominant-negative mutants of IKK and IKK , and an IκB ... cytoplasm by interaction with members of a family of inhibitory proteins, inhibitors of κB (IκBs) [32] In response to extracellular stimuli, phosphorylation of IκB on serines 32 and 36 and of IκB on...
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Báo cáo y học: " Caspase-3-mediated cleavage of p65/RelA results in a carboxy-terminal fragment that inhibits IκBα and enhances HIV-1 replication in human T lymphocytes" ppsx
... p65wt-tag and ΔNH2p65-tag proteins were expressed and able to bind IκB (Fig 7b) In vitro binding affinity of translated proteins p65wt-tag and ΔNH2p65-tag to IκB According to the previous data, ... of increasing quantities of ΔNH2p65-tag protein in the 3T3-p65ko cell line – maintaining the p65wttag protein concentration as invariable – showed that NF B-dependent activation increased in ... a higher binding affinity than p65wt for IκB in vivo in PHA -activated PBLs that had been treated with LMB for hours (Fig 1b) Accordingly, the binding affinity of both the p65wt-tag and ΔNH2p65-tag...
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Học tiếng Anh qua bài hát: Warm and Fuzzy
... Big old cups of steaming cider Những ly cũ lớn đầy rượu táo nghi ngút Fall asleep by a cozy fire Chìm vào giấc ngủ bên lửa ấm cúng It’s the warm and fuzzy time of year Đó khỏanh khắc ... ấm cúng It’s the warm and fuzzy time of year Đó khỏanh khắc ấm cúng huyền ảo năm It’s the warm and fuzzy time of year Là khoảnh khắc ấm cúng huyền ảo năm Santa’s on his way! Ông già Noel đến!...
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Ebook : HYDRAULIC STRUCTURES 4TH EDITION BY P. NOVAK, A.I.B, MOFFAT, C. NALLURI AND R. NARAYANAN
... outfalls and the treatment of wave forces on pipelines in the shoaling region Change of title (from Scale models in hydraulic engineering) to Models in hydraulic engineering to include in the general ... response and in their interactive relationship with site hydrology and geology In recognition of this, and reflecting the relatively indeterminate nature of many major design inputs, dam engineering ... embankment and culminated in the Giritale and Kantalai embankments (23 m and 20 m high respectively), completed in ELEMENTS OF DAM ENGINEERING AD 610 The Japanese and Indian entry into major dam building...
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Organizational behavior i essential theories of motivation and leadership by john b miner
... organizational behavior in business and other organizational settings; rather, most are concentrated in academia— teaching, writing, and conducting research In my opinion this is unfortunate; the field ... Improving Understanding and Modeling Academy of Management Review, 19, 230–51 Dubin, Robert (1976) Theory Building in Applied Areas In Marvin D Dunnette (Ed.), Handbook of Industrial and Organizational ... of designing and conducting good experiments that were so easily handled in the laboratory now become overwhelming Real organizations have innumerable ways of resisting and undermining objective...
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Organizational behavior i essential theories of motivation and leadership by john b miner
... organizational behavior in business and other organizational settings; rather, most are concentrated in academia— teaching, writing, and conducting research In my opinion this is unfortunate; the field ... Improving Understanding and Modeling Academy of Management Review, 19, 230–51 Dubin, Robert (1976) Theory Building in Applied Areas In Marvin D Dunnette (Ed.), Handbook of Industrial and Organizational ... of designing and conducting good experiments that were so easily handled in the laboratory now become overwhelming Real organizations have innumerable ways of resisting and undermining objective...
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ĐỀ THI THỬ ĐẠI HỌC NĂM 2012 - 2013 Môn Sinh học Khối B - Đề số 5
... xuất insulin điều trị bệnh tiểu đường nhờ trực khuẩn E coli, ADN tái tổ hợp gồm A tế bào E.coli có gen mã hóa insulin của người B nhiễm sắc thể E.coli và gen mã hóa insulin C gen mã hóa insulin ... ăn sau : Sinh vật sản xuất (2,1.106 calo) → sinh vật tiêu thụ bậc (1,2.104 calo) → sinh vật tiêu thụ bậc (1,1.102 calo) → sinh vật tiêu thụ bậc (0,5.102 calo) Hiệu suất sinh thái sinh vật tiêu ... dinh dưỡng sinh vật chuỗi thức ăn B quan hệ dinh dưỡng sinh vật loài quần xã C quan hệ dinh dưỡng sinh vật loài khác loài D quan hệ dinh dưỡng nơi sinh vật quần xã Câu 26 Ở loài thực vật, A: thân...
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