ii— origin of protein

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

Ngày tải lên : 19/02/2014, 00:20

... domain of DESC1 Scheme Domain organization of human DESC1 membrane region The extracellular part of DESC1 consists of a 120-amino acid SEA domain followed by the C-terminal trypsin-like serine proteinase ... development of speciﬁc inhibitors of individual TTSPs may provide insight into the molecular mechanisms of carcinogenesis as well as the normal biological roles of this interesting, emerging class of ... subgroups of the HAT ⁄ HAT-like ⁄ DESC and corin subfamilies exists We therefore cloned, expressed and puriﬁed the serine proteinase domain of DESC1 and solved the crystal structure of the complex of...

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Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt

Ngày tải lên : 15/03/2014, 09:20

... mixture had a volume of 10 lL and contained either 15 lg of total RNA or 0.125 A260 units (equivalent of lg of single-stranded RNA) of RNP particles and 0.2 pmol (300 000 c.p.m.) of [5¢-32P]-labeled ... transcription of the inserted intron RNA, contains sequence motifs characteristic of the H-N-H family of endonucleases, interspersed with two pairs of cysteine residues [11,14,15] Deletion of the conserved ... important protein region Effect of mutations in the C-terminal region of the RmInt1 IEP on RNA splicing in vivo We constructed a series of mutants to identify the functional features of the C-terminal...

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Báo cáo khoa học: The thermodynamic analysis of protein stabilization by sucrose and glycerol against pressure-induced unfolding The typical example of the 33-kDa protein from spinach photosystem II docx

Ngày tải lên : 31/03/2014, 01:20

... change of the protein is )43 mLÆmol)1 which might be the DV minimum of the protein (or of the system: protein, solvent and sucrose) in the presence of sucrose From this value, the P1/2 of the protein ... Stabilization of 33-kDa protein of spinach PS II against pressure unfolding (Eur J Biochem 270) 1657 Fig The eﬀect of sucrose (m), glycerol (j) and salt (d) on the standard volume change of the protein ... pressure-related studies on a larger set of globular proteins are required to determine the validity of each of the possible explanations [31] The contraction of the protein solvent interface caused...

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Báo cáo khoa học: ˚ Crystal structure at 3 A of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose pdf

Ngày tải lên : 31/03/2014, 01:20

... (1993) Molecular mechanism of inhibition of mammalian protein synthesis by some four-chain agglutinins: proposal of an extended classiﬁcation of plant ribosome-inactivating proteins (rRNA N-glycosidases) ... bonds with the O of Gly32, are not conserved in RTB and ABB However, Oc of Ser111 is located in a similar position to Oc1 of Thr110 of RTB Among the hydrophobic contacts, Ilel14 of MLB is mutated ... Tyr76 and Tyr115 in the active site of the A-chain, and Asp23 in the N-terminal sugar-binding site of the B-chain are shown in red The B-chain of MLI (MLB) consists of two homologous ˚ globular domains...

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Báo cáo khoa học: Modeled ligand-protein complexes elucidate the origin of substrate speciﬁcity and provide insight into catalytic mechanisms of phenylalanine hydroxylase and tyrosine hydroxylase pptx

Ngày tải lên : 31/03/2014, 07:20

... the catalytic domains of PAH and TH Docking of the native cosubstrate BH4 into the crystal structure of the PAH catalytic domain yielded a total of 286 conformations Out of these, 44 conformations ... position of tyrosine However, the orientation of BH4 in the secondranking conformation of tyrosine in THÆBH4 corresponded to that of 7,8-BH2 cocrystallized with TH [13] and in this orientation, N3 of ... the catalytic site of PAH (B) High-scoring positions of L-DOPA (red) and DA (blue, green) docked into the catalytic site of TH Ó FEBS 2003 Modeled ligand protein complexes of PAH and TH (Eur...

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Báo cáo y học: "Distribution patterns of small-molecule ligands in the protein universe and implications for origin of life and drug discovery" doc

Ngày tải lên : 14/08/2014, 08:20

... Björklund ÅK, Elofsson A: What properties characterize the hub proteins of the protein- protein interaction network of Saccharomyces cerevisiae? Genome Biol 2006, 7:R45 Prachumwat A, Li WH: Protein function, ... many proteins bind ligands that are essential for their functions and the ligands are likely to have originated independently of proteins [56-59], the binding of ligands with primordial proteins ... the origin of life Thus, it is intriguing to explore the chronology of ligand -protein binding and answer the following questions: which ligand was first recognized by a protein and what kind of...

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FOAMING PROPERTIES OF PROTEINS.pdf

Ngày tải lên : 16/12/2012, 15:10

... capacity of protein and the stability and strength of the foam Foaming ability i.e Gi denotes the amount of gas retained in the foam reflecting the potential of the protein for foaming The measures of ... closer packing of protein at the air/liquid interface and increased viscosity of the adsorbed protein layer (Mita et al., Table 3-Regression coefficients of foaming characteristics of ovalbumin ... evaluation of the effect of pH on foam character- TEMP IEC i!00mfi FOAM STRENGTH PROTEIN ,316 % ElEl I q.s 3.0 PH OF PROTEIN s.2 ! 3.14 SOLUTIDN Fig 6-Effect of pH and protein content on drainage...

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THE ORIGIN OF ENGLISH

Ngày tải lên : 07/07/2013, 01:27

... history of English begins a little after A.D 600 English is a Germanic Language of the Indo –European Family Indo – European English French Latin Greek Swedish Russian Hindi History of English ... inhabit, Warigeath, wulf –hleothu, windige Wolf –cliffs wild and windy headlands, naessas, Ledges of mist Where and mountain Frence fen –gelid, there forge –steam torrents Under naessas genipap, ... system & grammar wasn’t so effected but vocabulary was effected much Middle English A Clerk ther was of Oxenford also, That un –to logik hadde longe y –go Modern English A Clerk that is an Oxford scholar...

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Information, Entropy, and the Origin of Life

Ngày tải lên : 01/11/2013, 07:20

... the diversity of life in the biosphere, it is generally recognized that the origin of life is one of the great unsolved mysteries in science (Radetsky1992; Wade 2000) At the heart of this mystery ... the use of information, to survive and exist in a nonequilibrium state, resisting the pull toward equilibrium that is described by the Second Law of Thermodynamics For them, the origin of life ... are able to sustain themselves against the downward pull of the Second Law of Thermodynamics and how thermodynamics affects the origin of information-rich, living systems Finally, we will explore...

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Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

Ngày tải lên : 16/02/2014, 14:20

... pathlength of mm Because of these opposing effects, the ﬂuorescence of the peptide is quenched, and it contributes a smaller proportion of the total ﬂuorescence of the system However, in the presence of ... mechanisms of inhibition of PDI have been investigated Furthermore, since the original reporting of the inhibition of PDI by bacitracin [8], concerns have been raised about protease contamination of ... chains of a folding protein or with the hydrophobic binding site of molecular chaperones such as BiP, PDI and DsbC To further study the mechanism of action of bacitracin, the reduction of insulin...

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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

Ngày tải lên : 18/02/2014, 16:20

... mitochondria of Su9-DHFR, in which the presequence of ATPase subunit of Neurospora crassa was fused to a passenger protein, DHFR As seen in Fig 5A, the level of mitochondrial targeting of Su9-DHFR, ... Role of PKA in the mitochondrial targeting of CYP2E1 The role of PKA in mitochondrial targeting of CYP2E1 was investigated using two approaches The ﬁrst approach involved measuring the level of ... translocase of inner membrane 23 (TIM23) [18–20] Our studies deﬁned two distinct mechanisms of activation of cryptic mitochondria-targeting signals at the N-terminus of mammalian CYP proteins [4,10]...

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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt

Ngày tải lên : 18/02/2014, 16:20

... analysis of peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of Ser ... versions of the L34 protein are present in a ratio of : 20 Analysis of the spectra recorded on the other peaks in the base peak ion chromatogram allowed us to detect and identify 46 of the 55 proteins ... near the C-terminus of the protein As a result, the ETD spectrum is devoid of low mass fragments of type z¢Æ that would provide sequence information at the C-terminus of the protein Although this...

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Tài liệu Báo cáo khoa học: Applications of diagonal chromatography for proteomewide characterization of protein modiﬁcations and activity-based analyses doc

Ngày tải lên : 18/02/2014, 16:20

... et al COFRADIC and protein modiﬁcations analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ... of ATP-binding proteins As expected, the majority of FSBA-labeled Jurkat proteins were known binders of small nucleotides, cofactors, or DNA and RNA molecules However, several proteins and sites ... concentrate on applications of COFRADIC in studying selected post-translational modiﬁcations ) protein processing [34,36] and N-glycosylation [39] – and describe the use of COFRADIC for studying interactions...

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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll ﬂuorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc

Ngày tải lên : 19/02/2014, 05:20

... superposition of the exponential rise to analyze quantitatively the contribution of each phase The amplitude and rate constant of each of the three phases can be calculated by deconvolution of the traces ... thylakoids in the absence of additives or in the presence of lm DCMU or dPQ over a range of temperature from 15 C to 25 C The range of temperature was set on the basis of the membrane transition ... Boisvert et al have attributed both of the two latter phases to the reduction of the acceptor side of PSII [9,12,13], or more specically to the reduction of two distinct plastoquinone (PQ) pools...

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Tài liệu Báo cáo khoa học: Interferon-a induces sensitization of cells to inhibition of protein synthesis by tumour necrosis factor-related apoptosis-inducing ligand ppt

Ngày tải lên : 19/02/2014, 06:20

... of TRAIL such that very little of the full-length form of either protein remained in the IFN-treated cells after h of exposure to TRAIL Thus, the activity of caspase-8, and most likely that of ... only the extent of apoptosis but also the ability of TRAIL to regulate the initiation of translation at the level of eIF2a phosphorylation and 4E-BP1 dephosphorylation Control of protein synthesis ... Effects of IFNa treatment on the sensitivity of cells to inhibition of protein synthesis by TRAIL We have previously shown that protein synthesis is rapidly downregulated following exposure of cells...

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Tài liệu Báo cáo Y học: Prediction of protein–protein interaction sites in heterocomplexes with neural networks ppt

Ngày tải lên : 21/02/2014, 15:20

... structure of some of the complexes or by other experimental means CONCLUSIONS We have analysed the possibility of predicting the residues forming part of protein protein interacting surfaces in proteins ... Proteins 41, 47–57 16 Valdar, W.S.J & Thornton, J.M (2001) Protein protein interfaces: analysis of amino acid conservation in homodimers Proteins 42, 108–124 Ó FEBS 2002 Predicting protein protein ... 12 Chothia, C & Janin, J (1975) Principles of protein- protein recognition Nature 256, 705–708 13 Jones, S & Thornton, J.M (1997) Analysis of protein protein interaction sites using surface patches...

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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt

Ngày tải lên : 21/02/2014, 15:20

... result of overexpression of the recombinant proteins leading to a compromised mitochondrial integrity rather then to an intrinsic property of the proteins This could explain the lack of regulation ... the oligomycin-resistant portion of respiration reﬂects the level of proton leak of mitochondria, the low level of respiration in the presence of oligomycin of L6 cells showed that they were ... proteins using a yeast-two hybrid system revealed that the C-terminals of UCP2 and UCP3 (but not that of UCP1) speciﬁcally interact with members of the 14.3.3 protein family [37] The 14.3.3 proteins...

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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx

Ngày tải lên : 22/02/2014, 04:20

... As shown in Fig 2A, approximately 20 lM of NO was produced by 10 lgÆmL)1 of LPS and 100 UÆmL)1 of IFN Under these conditions, detectable induction of iNOS protein was observed by Western blotting ... iNOS protein induction Effects of a-T and its derivatives on the enhancement by TS of LPS/IFN-induced NO production We compared the effects of a-T and its derivatives TA and TN with that of TS ... mode of actions of TS, we examined the effect of TS on LPS/IFNinduced NO production in VSMC We found that TS activated LPS/IFN-induced NO production in VSMC through enhancement of iNOS protein...

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Báo cáo khoa học: Neural retina leucine-zipper regulates the expression of Ppp2r5c, the regulatory subunit of protein phosphatase 2A, in photoreceptor development pdf

Ngày tải lên : 06/03/2014, 02:20

... subunit of protein phosphatase 2A0 Evidence for the existence of at least seven novel isoforms J Biol Chem 271, 2578–2588 McCright B, Rivers AM, Audlin S & Virshup DM (1996) The B56 family of protein ... slower-migrating DNA protein complexes in a dose-dependent manner (lanes and 5), whereas the control GST protein alone did not form DNA protein complexes (lanes and 3) The presence of Nrl in the protein DNA ... Pallas DC (2000) WD40 repeat proteins striatin and S ⁄ G(2) nuclear autoantigen are members of a novel family of calmodulin-binding proteins that associate with protein phosphatase 2A J Biol...

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Báo cáo khoa học: NF-jB regulates the transcription of protein tyrosine kinase Tec pdf

Ngày tải lên : 07/03/2014, 00:20

... the survival of developing B lymphocytes and the maturation of spleen B cells [41–43] Abnormal activation of NF-jB has been shown to be a hallmark of the development and progression of lymphoid ... relative levels of luciferase activity are shown Data are representative of three independent experiments (D) Expression of Tec promoter–luciferase reporter constructs in the liver of living mice ... regeneration [52] Of note is the fact that the Tec promoter behaves in a similar way in cell lines of both hepatic and hematopoietic origin This may indicate that the altered reactivity of liver cells...

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