... sample, crystals were obtained under the same condition as that of the WtDSD crystals The quality of these crystals was better than the WtDSD crystals or WtDSD crystals obtained in the presence of ... dimeric formsof Foldtype II PLP-dependent enzymes will allow examination of the plausible role of oligomeric state in these enzymes [22] In this paper, we report the first crystal structure of a ... Indian Institute of Science, supported by the Crystal structure of D-serine deaminase Department of Science and Technology (DST) and the Department of Biotechnology (DBT), Government of India The...
... determined the crystal structures of two enzymes, TVA I [4,5] and TVA II [6,7], both of which hydrolyze CDs and a polysaccharide pullulan We have also determined the crystal structure of a solute-binding ... structures of TvuCMBP–ligand complexes The crystal structures of TvuCMBP liganded with a-CD, b-CD and G4 have been determined at 1.7, 1.8 ˚ and 1.55 A resolutions, respectively (Table 1) The crystals of ... positions of electron density, however, appear to be similar to those of two glucose residues, consisting of c-CD bound to the C-domain of TvuCMBP–c-CD (Fig 2B) The glucose residues of c-CD have...
... already (finish) the first semester exams 40 Mr Binh (learn) Chinese at school, but he (forget) most of it 41 We (have) a party next Sunday Would you like (come)? 42 While my sister and I (watch) a ... don’t mind (wait) 44 Ha Long Bay in Viet Nam (be) a World Herritage Site It (visit) by millions of people every year 45 If Mr Lam (go) to Paris next week, he (see) the Eiffel Tower 46 I myself ... Main Road yesterday A boy (knock) down by a car Then he (take) to the nearest hospital 47 Most of the Earth’s surface (cover) by water 48 The first festival (hold) nearly eight hundred years...
... the……………………… of the whole city after winning the championship (proud) 62 It’s good beacause I canm use it……………………… ( convenient) 63 They……………………… with each other in the sportsmanship of the Olympic ... London keeping their cars to the left made Mai……………………… a lot (amaze) 66 The……………………… of this paper mill is one of the highest in our country (produce) 67 Your money will be refunded if the goods ... (successful) 75 This is a very……………………… story (interest) 76 He is very……………………… of the work he has done (pride) 77.One of the basic needs in meny ……………………… countries is water (develop) 78 Everyone...
... art 126 40 Id at art 124 41 Consideration of reports submitted by States parties under article 18 of the Convention on the Elimination of All Formsof Discrimination against Women, Combined initial, ... analyze the state of health and health-care programs from the perspective of the health ministry in each country One of the survey questions asked each country to rank, in order of priority, its ... and the Ministry of Health’s technical units on maternal mortality.27 The TCU found that all of the sources consulted unanimously admitted an under-evaluation of the number of maternal deaths.28...
... alone because of blended families Security Because of “blended families”, as a fact of the changes due to the growing participation of women in the labour force, and the high incidence of divorce ... arguable to live at home? What are there barriers for elderly to think of new possible formsof housing? Are new formsof housing well accepted? (market driven) To differentiate: maintain to ... they say, the overall well being of a person is influenced of the health, socioeconomic status, nationality and social interaction and the weighted importance of one of these depends on the specific...
... [55] Crystallization TaLcc1 was crystallized at room temperature with the hanging drop vapour diffusion method Two microlitres of protein solution at a concentration of mgÆmL)1 and lL of crystallization ... TaLcc1 crystal structure, molecules A and B (and C and D) of the asymmetric unit form a weak dimer (Fig 6) On the basis of calculations performed Fig (A) Cartoon representation of dimers of TaLcc1 ... Journal compilation ª 2011 FEBS 2285 Crystal structure of a Thielavia arenaria laccase J P Kallio et al thermostability of the enzyme [34] On the basis of the sequence of TaLcc1, there are eight putative...
... assessment of data quality Acta Crystallogr D: Biol Crystallogr 62, 72–82 38 McCoy AJ, Grosse-Kunstleve RW, Adams PD, Winn MD, Storoni LC & Read RJ (2007) Phaser crystallographic software J Appl Crystallogr ... through a suite of programs in the ccp4 crystallography package [36] Scaling of intensities and inspection of data quality were performed in scala In particular, the resolution cut-off was determined ... data Acta Crystallogr D: Biol Crystallogr 62, 48–57 36 Collaborative Computational Project Number (1994) The CCP4 suite: programs for protein crystallography Acta Crystallogr D: Biol Crystallogr...
... (Fig 3A) Three of the ligands, OD2 of Asp165, NE2 of His79 and NE2 of His169, formed an equatorial plane The other protein ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, ... conformation around the active site of ApeSOD is independent of the presence of a metal cofactor Figure 3D shows the superimposition of the active site structures of apo, Mn-bound and Fe-bound ApeSODs ... metal-reconstituted ApeSODs Crystallization and determination of structure The crystals of ApeSOD were grown for 2–3 days in the presence of polyethylene glycol The crystals belonged to the space group P21, with...
... potential provider of support to so This probability of support indicates a minimum Long-Term Rating floor of ‘BB-’ Fitch Ratings – Definitions of Ratings and Other Formsof Opinion – Nov 2012 ... presumption of potential support being forthcoming In practice this approximates to a probability of support of less than 40% Fitch Ratings – Definitions of Ratings and Other Formsof Opinion ... Ratings – Definitions of Ratings and Other Formsof Opinion – Nov 2012 CCC: Substantial credit risk Default is a real possibility CC: Very high levels of credit risk Default of some kind appears...
... Content of the asymmetric unit Number of nonhydrogen atoms Number of protein molecules Number of water molecules Number of glycerol molecules Number of sodium ions Number of magnesium ions Number of ... Drops consisted of 1.5 lL of protein solution (5 mgÆmL)1) and 1.5 lL of crystallization buffer The crystals grew within week at 22 °C Data collection and processing For cryoprotection crystals were ... al ¨ Crystal structure of Klebsiella phytase PhyK Binding model A crystal structure of the inactive mutant H25A of PhyK was determined for four molecules in the asymmetric unit The exchange of...
... Asymptotic behavior of all elements of the matrix occurs whenever all of the roots of the characteristic equation of A have negative real parts where the characteristic equation of A is defined det( ... mode of oscillation is commonly referred to as the hunting or swing mode, which is the principal mode of oscillation of the rotor of the machine relative to the electrical angular velocity of the ... oscillation of the steam unit (Fig 5.10-10) is more damped and of higher frequency than the corresponding rotor oscillation of the hydro unit The real eigenvalues are associated with the decay of the offset...
... the presence of lm ACMS substrate Crystallization and structure determination Crystals of hACMSD were obtained using the vapour diffusion technique in hanging drop A volume of lL of reservoir ... axis in the crystal, and a mixture of monomeric and dimeric forms in solution [18] Therefore, the available structural data suggest Fig Ribbon representation of the overall structure of hACMSD ... design of enzyme inhibitors with potential medical interest Crystal structure of human ACMSD C-ter N-ter Results and Discussion Overall quality of the model The three-dimensional structure of hACMSD...
... domain of TtAK contributes not only to catalytic regulation, but also the thermostability of TtAK Comparison of the crystal structures of TtAKb and CgAKb was expected to elucidate the mechanism of ... A Yoshida et al Crystal structures of regulatory subunit of Thr-sensitive aspartate kinase Fig Stabilization of oligomer formation of TtAK and TtAKb by Thr (A) Elution profiles of TtAKb in the ... elevated thermostability of TtAKb In this article, we describe the crystal structures of TtAKb in two forms, Thr-bound and Thr-free, and discuss the regulatory mechanism of Thr and the structural...
... of E coli in plate assay using 100 pmol of each peptide, complete disappearance of ferroportin signal at the membrane of iron treated macrophage J774 cells in the presence of 700 lM of each of ... microlitres of hepcidin (0.1 mgÆmL)1) were eluted with the following gradient at mLÆmin)1 from 90% of (H2O, 0.1% TFA) and 10% of (H2O 30%, CH3CN 70%, TFA 0.1%) to 10% of (H2O, 0.1% TFA) and 90% of (H2O ... removal of the heat denaturated proteins; lane 4, 30 lg of Co2+ TalonÔ unbound protein fraction; lane 5, 50 lg of Co2+ TalonÔ 150 mM imidazole eluted fraction, containing TRX-huhepc; lane 6, 40 lg of...
... Max-Planck-Insitute of Biochemistry Journal compilation ª 2007 FEBS 2157 Crystal structure of the catalytic domain of DESC1 O J P Kyrieleis et al screenings of crystallization conditions Initial crystal ... serine proteinase domain of DESC1 and solved the crystal structure of the complex of this protease with benzamidine Results and Discussion Overall structure The catalytic domain of DESC1 resembles ... Max-Planck-Insitute of Biochemistry Journal compilation ª 2007 FEBS 2149 Crystal structure of the catalytic domain of DESC1 O J P Kyrieleis et al Fig Stereo ribbon representation of DESC1 in complex...
... of the substrate However, both the specific substrate of the enzyme and the biochemical pathway in which BcZBP is involved remain to be identified Crystal structure of BcZBP from B cereus The crystal ... part of the structure Although five of its residues could not be located to the TT1542 model, the electron density map of BcZBP was interpretable for all residues of this loop in both chains of ... established by the incorporation of the b8-strand of one monomer into a b-sheet of the other monomer The position of the zinc ion is indicated by a circle (B) Schematic diagram of the dimer Each monomer...
... key features of PDH specificity and mode of inhibition of the enzyme Results and Discussion Structural analysis and model quality Crystal structures of three different complexes of LlPDH have ... movement of the cofactor-binding domain of subunit A relative to subunits B and C (not shown) This domain alteration involves a rotation of 5° and translation of ˚ 0.7 A Five segments of polypeptide ... hydrogen bond with the C3-hydroxyl of RU5P, C2-hydroxyl of PEX or the C4-hydroxyl of 6PG (in the modelled ternary complex) Some 84 residues of the cofactor-binding domain of LlPDH are strictly conserved...
... mature formsof D164N and E78H ⁄ D164N, respectively, were subjected to X-ray A crystallographic studies Crystals of the D164N mutant of kumamolisin-As were fully isomorphous with those of the ... The rate of ligation was estimated from percentage intensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and 19-kDa bands, where the intensity of the 38-kDa ... indicate relative percentage of kcat and kcat ⁄ Km values of mutants, with those of wildtype enzyme taken to be 100% For E78H ⁄ D164N, a mixture of the nicked and intact formsof the precursor could...