identifying specific trans factors of rna editing in plant mitochondria by multiplex single base extension typing
Functional characterization of RNA editing and alternative splicing in the carboxyl terminus of cav 1 3 calcium channel
... Developmental profile of editing in ADAR2 substrates in mouse brain Figure 3.3 Spatial profile of editing in ion channels in adult mouse Figure 3.4 Spatial profile of editing in ADAR2 substrates in adult ... calcium-dependent inactivation (CDI) 1.3 RNA editing Pre-mRNA editing by selective adenosine deamination is catalysed by Adenosine Deaminases Acting on RNA (ADARs), resulting in a single nucleotide ... 1.2.3 The carboxyl terminal domain 3 1.3 RNA editing 1.3.1 Adenosine Deaminase Acting on RNA (ADAR) 1.3.2 Mechanism of RNA editing 1.3.3 Substrates of RNA editing 1.3.4 Role in neurophysiological...
- 174
- 400
- 0
Báo cáo khoa học: Trans-splicing of a mutated glycosylasparaginase mRNA sequence by a group I ribozyme deficient in hydrolysis pptx
... calculated by dividing the transsplicing band (RNA 2) ·100 by the sum of the trans- splicing band (RNA 2) and the major GA band (RNA 3) As the amount of DiGIR2 AGU and DiGIR2DP9.2 AGU ribozymes added in ... upstream of U436 in mutated GA mRNA and to a 49 nt region of the 3¢ exon in the ribozymes, resulting in a 361 nt protected region for the trans- spliced RNA The probe was in vitro transcribed containing ... probe; RNA 2, trans- spliced GA mRNA; RNA 3, major GA band; RNA 4, major DiGIR2 AGU and DiGIR2DP9.2 AGU band Additional bands result from degradation of RNA during incubation at trans- splicing and...
- 7
- 307
- 0
báo cáo hóa học: " Identifying type and determinants of missing items in quality of life questionnaires: Application to the SF-36 French version of the 2003 Decennial Health Survey" pot
... (5-10 min) and welladapted for studies in general populations Strategy for identification of type and determinants of missingness The type of missingness was defined according to Little and Rubin ... and Rubin to characterize missing data processes operating during administration of this questionnaire In this large representative sample of the French population the proportion of missing items ... Machin D: Incomplete quality of life data in randomized trials: missing items Statistics in Medicine 1998, 17:679-96 Perneger TV, Burnand B: A simple imputation algorithm reduced missing data in...
- 6
- 471
- 0
Báo cáo y học: " Identification of fusion genes in breast cancer by paired-end RNA-sequencing" potx
... base pair; DNA-seq: DNA-sequencing; FISH: fluorescence in situ hybridization; RNAi: RNA interference; RNA- seq: RNA- sequencing; siRNA: small interfering RNA; UTR: untranslated region Acknowledgements ... possibility of mRNA trans- splicing in some of the cases [15] Association with copy number breakpoints Integration of RNA- seq with array comparative genomic hybridization (aCGH) data showed that, in 23 of ... membrane protein-associated protein B and C) and the carboxyterminal part of IKZF3, which harbors two Zn-finger domains IKZF3 was only detected as a fusion transcript, indicating activation of a quiescent...
- 13
- 439
- 0
Báo cáo y học: "Subunit-specific mutational analysis of residue N348 in HIV-1 reverse transcriptase." docx
... repositioning of this loop in the N348I RT is likely due to the bulky sidechain of isoleucine disrupting the network of interactions between this residue and Y342 Similarly, the introduction of leucine ... RNase H domain that form part of the nucleic acid binding tract are shown in spacefill (and colored according to domain color) B) Location of the b14-b15 loop in the p51 subunit of HIV-1 RT in ... illustrating the location of the RNase H cleavage sites in the RNA template B) Autoradiogram of the RNase H cleavage patterns of HIV-1 RTs containing mutations at residue 348 in both subunits of the...
- 9
- 396
- 0
Identifying protein co factors of oct4, an essential stemness transcription factor, by affinity purification and mass spectrometry
... points In eukaryotes, basal transcription of genes is carried out by different types of nuclear RNA Polymerases (RNAP), depending upon the specific type of product involved (RNAPI ribosomal RNA, ... potential DNA binding sites for proteins of interest Chromatin Immunoprecipitation has been used as a means of detecting where proteins of interest might bind to specific genomic regions in vivo Briefly, ... bind cooperatively to the nucleosome-free region of the IFN-β promoter The binding of individual factors is insufficient for transcriptional activation, but the binding of all transcription factors...
- 209
- 293
- 0
An error analysis of using reference in written english by secondary school studend = phân tích lỗi của học sinh THPT tong việc sử dụng phép quy chiếu trong tiếng anh viết
... capability of using language Finding and analyzing errors by learners; therefore, are significant to improve learning and teaching quality Error analysis (EA) has been defined by many linguists in different ... Basing on the analysis of errors done by linguists and researchers, errors are classified into two main types: They are interlingual errors caused by the interference of the mother tongue and intralingual ... that students commit a lot of errors in using reference and they have great difficulties in using types of reference There are some following specific findings found in this study - The students...
- 51
- 613
- 0
Báo cáo khoa học: Evidence for the presence of ferritin in plant mitochondria pdf
... antibody against ferritin (PAAF) (C) Immunoblotting of recombinant human mitochondrial ferritin (rHuMt, 10 ng), protein extract from HeLa cells overexpressing human mitochondrial ferritin (MtF-HeLa, ... mass of 25–26 kDa was revealed, thus suggesting the presence of this iron-storage protein also in mitochondria from this type of plant cells Furthermore, preliminary results indicate that ferritin ... from the binding of the inhibitor protein IF1 Biochim Biophys Acta 1556, 133–141 25 Shevchenko, A & Shevchenko, A (2001) Evaluation of the efficiency of in- gel digestion of proteins by peptide...
- 8
- 504
- 0
DETERMINATION OF ORGANIC COMPOUNDS IN DRINKING WATER BY LIQUID-SOLID EXTRACTION AND CAPILLARY COLUMN GAS CHROMATOGRAPHY/MASS SPECTROMETRY pdf
... sample Ais = integrated abundance of the quantitation ion of the internal standard in the sample Qis = total quantity (in micrograms) of internal standard added to the water sample V = original water ... used in this method as internal standards, are rapidly oxidized and/or chlorinated in water containing residual chlorine Therefore, residual chlorine must be reduced at the time of sampling These ... part of the performance test The correct setting of the baseline threshold, as indicated by the presence of low intensity ions, is the next most important part of the performance test Finally,...
- 60
- 528
- 0
Báo cáo khoa học: Reannotation of hypothetical ORFs in plant pathogen Erwinia carotovora subsp. atroseptica SCRI1043 pot
... methyltransferases Protein involved in catabolism of external DNA Addiction module toxin, RelE ⁄ StbE family Antitoxin of toxin-antitoxin stability system YicC N-terminal domain protein Twin-arginine ... were based on the larger training set Using the final Fisher coefficients and the criterion for deciding coding ⁄ noncoding, the hypothetical ORFs in Eca1043 were re-identified A total of 49 of the ... distinguishing between protein-coding and noncoding ORFs The difference between coding and noncoding sequences can be viewed intuitively using PCA PCA defines the correlation among the variables of...
- 9
- 170
- 1
Báo cáo khoa học: Translation initiation region dependency of translation initiation in Escherichia coli by IF1 and kasugamycin pdf
... fMet-tRNA binding to the P-site [10,12,13], probably by stabilizing IF2 binding to the 30S subunit [14], and stimulates the GTPase activity of IF2 [15] It also increases binding of mRNA to the initiation ... kasugamycin-binding site on the 30S subunit or on the 70S ribosome in the region of the mRNA-binding tunnel in the E-site and P-site [32,33] As no effect of kasugamycin on mRNA binding to the ... nucleotides in mRNA corresponding to the E-site [32] Translation of leaderless mRNA starting directly from AUG is insensitive to kasugamycin action [37] TIR dependence of translation initiation by IF1...
- 12
- 484
- 0
Báo cáo khoa học: Effects of various N-terminal addressing signals on sorting and folding of mammalian CYP11A1 in yeast mitochondria doc
... phosphoglycerate kinase promoter In this case the content of recombinant protein in mitochondria was reduced by an order of magnitude However, the bulk of CYP11A1 was again in the form of aggregates insoluble ... content of the imported protein in the supernatant was 0.025% of total protein of the fraction, whereas that in mitochondria was approximately 0.3% of total protein Obviously, the supernatant containing ... CoxIV(1–25)-mCYP11A1 in whole mitochondria and the corresponding 12 000 g supernatant determined by SDS/PAGE and Western blotting of the samples containing equal amounts of total protein According to these...
- 8
- 470
- 0
origin of coherent phonons in bi2te3 excited by ultrafast laser pulses
... the thinner films still have crystalline structure, as shown in the Raman scattering data in Fig 4(c) The widths of the Raman peaks in the thinner films are slightly wider, indicating longer interatomic ... perturbations are introduced to the molecular system by slightly displacing the atomic positions along the directions indicated in Fig For example, the A1 phonon 1g mode is generated in MD by stretching the ... anharmonicity in thinner films The band gap in 064307-4 ORIGIN OF COHERENT PHONONS IN Bi2 Te3 PHYSICAL REVIEW B 88, 064307 (2013) the very thin Bi2 Te3 films can be wider, for example, ∼0.25 eV in 5-nm-thick...
- 6
- 345
- 0
origin of coherent phonons in bi2te3 excited by ultrafast laser pulses
... the thinner films still have crystalline structure, as shown in the Raman scattering data in Fig 4(c) The widths of the Raman peaks in the thinner films are slightly wider, indicating longer interatomic ... perturbations are introduced to the molecular system by slightly displacing the atomic positions along the directions indicated in Fig For example, the A1 phonon 1g mode is generated in MD by stretching the ... anharmonicity in thinner films The band gap in 064307-4 ORIGIN OF COHERENT PHONONS IN Bi2 Te3 PHYSICAL REVIEW B 88, 064307 (2013) the very thin Bi2 Te3 films can be wider, for example, ∼0.25 eV in 5-nm-thick...
- 6
- 370
- 0
báo cáo hóa học: "Reduction of motion artifact in pulse oximetry by smoothed pseudo Wigner-Ville distribution" doc
... weighted moving average (WMA) By calculating the ratio of the AC components and the ratio of the DC components of the two light sources, SpO2 can be obtained from every single pulse of a PPG signal ... Scharf JE, Sankar R: Alternate pulse oximetry algorithms for SpO2 computation Proceedings of the Sixteenth Annual International Conference of the IEEE Engineering in Medicin and Biology Society ... samples in every several-second intervals In this study, the SpO2 obtained by the WMA approach was the average of SpO2 samples in an 8-second period Overlap processing was performed at 1-second interval...
- 9
- 371
- 0
Báo cáo hóa học: " Study of Materials Deformation in Nanometric Cutting by Large-scale Molecular Dynamics Simulations" docx
... movement, and cutting forces during the cutting processes of no-wear, adherence, plowing, and cutting regimes; Komanduri et al [5–7] carried out MD simulations of nanometric cutting of single- crystal ... the start of the cutting Then the cutting forces tend to remain steady during the rest of the cutting process The formation of dislocations results in the release of the accumulated cutting energy, ... are maintained at 300 K by rescaling the velocities of the atoms The velocity Verlet algorithm with a time step of fs is used for the time integration of Newton’s equations of motion The interatomic...
- 8
- 275
- 0
STATE OF THE ART IN BIOMETRICS Edited by Jucheng Yang and Loris Nanni pdf
... processes of a fingerprint matching algorithm is presented in Figure Fig General processes for minutiae-based fingerprint matching In minutiae-based matching, minutiae are commonly represented as minutiae ... noisy resulting in difficulty in determining dominant ridges and orientations This, in turn, makes the orientation certainty level of the fingerprint lower than that of a normal one Kinetic Sciences ... over the age of 75 due to the lack of definition in the ridges on the pads of the fingers Purdue University has made several inquiries into the image quality of fingerprints and fingerprint recognition...
- 326
- 515
- 0
Báo cáo khoa học: "Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR" pot
... methods include H E staining and silver staining of the intestinal sections 36 Dong-Kyun Suh et al Fig Section of the ileum from a pig infected with L intracellularis a : Presence of numerous intracellular, ... one of the important factors for increasing the sensitivity of the PCR assay The increase in the number of amplification cycles may produce nonspecific DNA But nonspecific DNA was not detected in ... availability of the PCR assay for screening the prevalence of L intracellularis infection in the pig farms Macroscopic Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR examination...
- 5
- 288
- 0
Báo cáo y học: "α Does protein kinase R mediate TNF-α- and ceramide-induced increases in expression and activation of matrix metalloproteinases in articular cartilage by a novel mechanism" pps
... significant, increase in levels of proMMP-2 and active MMP-2 in the media C2-ceramide-induced regulation of MMP-2 is abrogated by inhibition of PKR R48 To determine a role for PKR in ceramide-induced ... in transcriptional pathways activated by specific cytokines [21,26], growth factors [27], and extracellular stresses [28] PKR is involved in a number of cellular responses, including signal transduction, ... to the induction of c-jun and c-fos and AP-1-dependent transcription of MMP genes [10] In addition, triggering of the ceramide pathway in human keratinocytes results in overexpression of MMP-9...
- 10
- 379
- 0
Báo cáo y học: "Altered expression of inflammatory cytokines in primary osteoarthritis by human T lymphotropic virus type I retrovirus infection: a cross-sectional study" pps
... or definite osteophytes with moderate joint space narrowing; IV, severe with large osteophytes and definite joint space narrowing bScoring was as follows: +/-, minimum staining in one area of the ... tissue; +, patchy staining involving several areas; ++, moderate diffuse staining OST, osteotomy; SYV, synovectomy; TA, total joint arthroplasty Of the cytokines examined in our study, C-PTHrP ... Kellgren/Lawrence; M, male Inc.) Two envelope proteins and three core proteins derived from HTLV-I were fixed onto nitrocellulose membrane, and specific binding of IgG or IgM was distinguished by specific secondary...
- 8
- 601
- 0
Tìm thêm: xác định các mục tiêu của chương trình khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25