identification of genes involved in tumor like properties of embryonic stem cells pdf
Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The ... destruction of additional human embryos Indeed, the embryos from which the existing ESCs originated have already been destroyed Federal funding of medical research on these existing stem cell lines...
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Báo cáo khoa học: The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells pdf
... amount of glycoprotein in cells As shown in Fig 5E, although the total amount of protein in cells decreased to 87% after 48 h of incubation, the amount of glycoprotein decreased more The results of ... with 200 lL of lipofectamine solution, dissolving lL of Lipofectamine 2000 in Opti-MEM, and transfected into subconfluent 293A cells in mL of Opti-MEM in six-well plates The 293A cells were then ... and wild-type mouse embryonic fibroblast cells (WT cells) were grown in DMEM containing 20% fetal bovine serum CDG-Ib patient cells were grown in a-MEM containing 10% fetal bovine serum All media...
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báo cáo khoa học:" Induction of osteogenic markers in differentially treated cultures of embryonic stem cells" ppt
... xenogenic cells [13-16] There are some reports that use totipotential embryonic stem cells in tissue engineering of bone [17,18] Embryonic stem cells (ESCs) are routinely derived from the inner cell ... are von Kossa stainings with arrows pointing to the deposition of calcium phosphate-containing minerals that stained in black Figure LIF, stem cell PCR showing amplification of the without hematopoeticqualitative ... adipogenesis in embryonic stem cells by bone morphogenetic protein-2: effect of cofactors on differentiating lineages BMC Dev Biol 2005, 5(1):1 Bielby RC, Boccaccini AR, Polak JM, Buttery LD: In...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ES cells in epigenetic ... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... Fig Involvement of Dnmts in establishing and maintaining DNAmethylation profiles Dnmt3a ⁄ 3b have an indispensable role for maintaining DNA methylation in gene areas, which are constituents of...
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differentiation of embryonic stem cells
... of Megakaryocytes from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of Embryonic ... YAMAZAKI, AND SHIN-ICHI HAYASHI 98 v vi TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils ... an impure mixture of matrix proteins derived from porcine or bovine skin, on the growth of pluripotent cells are often overlooked Gelatin can increase the differentiation of EPL cells when compared...
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The signals of FGFs on the neurogenesis of embryonic stem cells ppsx
... re-initiated the ES differentiation and neurogenesis, indicating that the FGF pathway participates in the initiation of ES commitment into embryonic cell lineages List of abbreviations ESC: embryonic ... Nuclei of all cells are revealed by DAPI staining in blue Scale bar, 10 μm in C *, p < 0.01, Anova test into neuroblast cells Immunocytostaining revealed that both FGFR1 and FGFR3 were detected in ... phosphorylated isoforms of JNKs and ERKs in the differentiating ES cells, respectively Specific pharmacological inhibitors of MAPKs, shown affecting their respective kinase targets in Fig 4B, were administrated...
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báo cáo khoa học: " Transcriptomic identification of candidate genes involved in sunflower responses to chilling and salt stresses based on cDNA microarray analysis" pps
... stress per se In another study the expression of 150 genes in response to wounding and insect feeding was carried out [58], finding that some of the responding genes, for which the inducing stimulus ... http://www.biomedcentral.com/1471-2229/8/11 elements binding proteins) TFs While cis elements in cold response genes bind DREB1/CBF TFs, regulatory regions of drought response genes bind TFs belonging to DREB type protein [5,31-33] ... showing 287 genes whose expression levels were studied in three treatments: control (Ctrl), cold (C) and salinity (S) The ordination was obtained taking into account the three (two in case of...
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Báo cáo y học: "The ASRG database: identification and survey of Arabidopsis thaliana genes involved in pre-mRNA splicing" doc
... proteins, such as SF1/BBP (branchpoint-binding protein, which facilitates U2 snRNP binding in fission yeast [54]) and cap-binding proteins (CBP20 and CBP80, possibly involved in cap proximal intron ... ribonucleoprotein (Sm protein); S_Tkc: Serine/threonine protein kinase; Surp: SWAP/Surp; Thioredoxin_2: Thioredoxin domain 2; TPRlike: TPR -like; TPR: TPR repeat; Tudor: Tudor domain; TyrKc: Tyrosine protein ... N-terminal beta-propeller domain; PfkB_Kinase: Carbohydrate kinase, PfkB; PHOSPHOPANTETHEINE: Phosphopantetheine attachment site; Pinin/SDK/memA: Pinin/SDK/ memA protein; Pkinase: Protein kinase;...
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ConstructionandCharacterizationofaFulllengthcDNALibraryandIdentificationof GenesInvolvedinSalinityStressinWild Eggplant(SolanumtorvumSwartz)
... Vigor’ ESTs Seven genes that contained the initiation codon (ATG) of the open reading frame (ORF) were functionally assigned as proteins involved in salinity stress, including: betaine aldehyde dehydrogenase ... rate-limiting step in protein folding (Zhu et al., 2011) In plants, cyclophilin genes are induced in response to biotic or abiotic stresses It has been reported that cyclophilin proteins in tobacco ... ‘full-length’ if the 5’-deduced amino acid sequence (N-terminus) of this cDNA matched the initiation methionine of a ‘complete protein sequence’ in the NCBInr protein ([WUDFWLRQ RI 7RWDO 51$...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... ethanol-induced mucosal injury in the upper gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44] These factors are involved in ethanol-induced ... et al Genome-wide analysis of gene expression in human hepatocellular carcinomas using cDNA microarray: identification of genes involved in viral carcinogenesis and tumor progression Cancer Res ... necessary step in the understanding of the molecular mechanisms involved in alcohol-induced effect in human hepatocellular carcinoma cells In addition, this represents a novel approach for the identification...
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Tài liệu Báo cáo Y học: Identification of a set of genes involved in the biosynthesis of the aminonucleoside moiety of antibiotic A201A from Streptomyces capreolus pdf
... implicated in the biosynthesis of the aminonucleoside moiety of A201A Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicated in the biosynthesis of this ... transcription terminator The resulting fragment only contains the initial 67 bp and the final 68 bp of the pur4 coding sequence It was inserted in plasmid pGM9 The resulting construct was introduced, ... point, the procedure described in Hopwood et al [14] was followed DNA sequencing was made by the dideoxy-chain termination method [25] using the Amplitaq dye-terminator sequencing system (Perkin...
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Báo cáo khoa học: Amino acid limitation regulates the expression of genes involved in several specific biological processes through GCN2-dependent and GCN2-independent pathways ppt
... regulated in wild-type cells For example, the genes involved in amino acid metabolism were not regulated by amino acid starvation in GCN2) ⁄ ) cells, whereas enrichment for the genes involved in cholesterol ... is directly involved in the regulation of amino acid and protein metabolism, as many of the genes involved in these processes are not regulated in GCN2) ⁄ ) cells These results are in good agreement ... stabilization of mRNA This latter mechanism has been described for the amino acid-dependent regulation of several genes, including Chop, Atf3, Cat-1 and insulinlike growth factor binding protein (Igfbp1),...
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báo cáo khoa học: "Prospecting for Genes involved in transcriptional regulation of plant defenses, a bioinformatics approach" pdf
... kinases leading to repression of SA and induction of JA defense genes; yellow boxes, genes involved in JA biosynthesis; light blue boxes, genes involved in ET biosynthesis; pink boxes, genes involved ... (encoding ubiquitin ligases EOL1, ETO1) involved in ET biosynthesis, indicative of a possible function in the integration of ET and auxin signaling pathways The MAP kinases in the ET signaling pathway ... Within the plant field there is an increasing number of publications that report the finding of biologically relevant genes involved in certain pathways via coexpression analysis Examples are: genes...
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báo cáo khoa học: " High levels of nucleotide diversity and fast decline of linkage disequilibrium in rye (Secale cereale L.) genes involved in frost response" doc
... region; I: intron Failure of amplification in some of the lines may be due to the presence of SNPs/Indels in the binding sites of the sequences and/or the absence of some of the Cbf genes in some ... of amino acids of different polarity The first intron and second exon comprising 20% of the coding sequence of ScIce2 were amplified, resulting in the identification of 36 SNPs, all located in ... levels of all cold-induced Cbf genes at the frost tolerance locus Fr-H2 in barley are significantly higher in lines harbouring the vrn1 winter allele than in lines harbouring the Vrn1 spring allele...
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báo cáo khoa học: " Targeted isolation, sequence assembly and characterization of two white spruce (Picea glauca) BAC clones for terpenoid synthase and cytochrome P450 genes involved in conifer defence reveal insights into a conifer genome" pdf
... that increasing the sequencing depth beyond × coverage results in only a marginal improvement of the sequence assembly The future sequencing of a conifer genome will likely use a combination of ... features of conifer genomes pose untested challenges for the targeted isolation and sequence assem- http://www.biomedcentral.com/1471-2229/9/106 bly of BACs containing genes of interest involved in ... role in the regulation of the 3CAR gene The upstream regions of 3CAR and CYP720B4 contain putative cis-acting elements consistent with the roles of these genes in induced defence A large volume of...
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báo cáo khoa học: " Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in Camptotheca acuminata Decne (Nyssaceae)" ppt
... expression of genes involved in their biosynthetic pathway In C roseus, RNA in situ hybridization combined with immunocytolocalization techniques has demonstrated that the genes involved in the early ... flower bud, and in the apical meristem of the root tip, whereas the genes involved in the terminal stages (D4H and DAT) are expressed in the laticifer and idioblast cells of the leaf, stem and flower ... and camptothecin levels in Camptotheca acuminata seedlings Physiol Plant 1999, 105:402-408 27 Liu Z: Drought-induced in vivo synthesis of camptothecin in Camptotheca acuminata seedlings Physiol...
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Báo cáo y học: " Transcriptome profiling of primary murine monocytes, lung macrophages and lung dendritic cells reveals a distinct expression of genes involved in cell trafficking" pot
... Il18r1 interleukin alpha [NM_010554] interleukin [NM_031168] interleukin 18 [NM_008360] interleukin 17D [NM_145837] interleukin beta [NM_008361] interleukin 11 receptor, alpha chain [NM_010549] interleukin ... grouped into trafficking related clusters such as integrins, Mmps, chemokine and chemokine receptors, and interleukins and interleukin receptors Integrins are key mediators of cell-cell interactions, ... paid to genes involved in cell trafficking, namely integrins, metallopeptidases, chemokines and chemokine receptors, as well as interleukins and interleukin receptors (Table 1) In order to visualize...
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the regulation of genes involved in trichome development
... calmodulin-binding kinesin -like motor protein (Oppenheimer et al., 1997) ZWI interacts with two other proteins involved with trichome branching: ANGUSTAFOLIA and KCBP-interacting Ca2+ binding protein ... of epidermal cell fate determination by interactions between proteins involved in trichome patterning A combination of a MYB protein (either GL1 or MYB23) with a bHLH dimer (any combination of ... neighboring cells, where it would interact preferentially with the bHLH dimer, preventing expression of genes involved in trichome development TTG would act in the cytoplasm most probably aiding in...
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the regulation of genes involved in trichome development(fileminimizer)
... calmodulin-binding kinesin -like motor protein (Oppenheimer et al., 1997) ZWI interacts with two other proteins involved with trichome branching: ANGUSTAFOLIA and KCBP-interacting Ca2+ binding protein ... of epidermal cell fate determination by interactions between proteins involved in trichome patterning A combination of a MYB protein (either GL1 or MYB23) with a bHLH dimer (any combination of ... neighboring cells, where it would interact preferentially with the bHLH dimer, preventing expression of genes involved in trichome development TTG would act in the cytoplasm most probably aiding in...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
... recombinant Lefty1 protein was able to block reduction in level of alkaline phosphatase staining induced by Lefty2 RNAi Alkalline phosphatase staining was carried out day post transfection of Lefty2 ... were used in the selection of genes The first criterion was the expression level of the candidate genes in pluripotent cell types of both mouse and human, such as in ES cells in the in- vitro scenario, ... regulation of Lefty2 by Oct4, Sox2 and Nanog pluripotency genes in human ES cells All these observations fuelled my interest in further examining the functional role of Lefty2 in ES cells For...
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