identification detection and molecular characterization of novel monopartite begomoviruses
Báo cáo y học: " Molecular characterization of two distinct monopartite begomoviruses infecting tomato in india" pps
... prominently in the evolution of these viruses Page of 10 Results Identification, detection and molecular characterization of novel monopartite begomoviruses Leaves of infected tomato plants were ... case of bipartite begomoviruses or monopartite begomoviruses having betasatellite molecules When the IR sequence of K3/K5 and CTM isolates was compared and aligned to the published IR sequences of ... a molecular study of the sequences of genomes of different ToLCV isolates collected from the Indian subcontinent in an effort towards identifying and determining the molecular variability of...
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Báo cáo khoa học: "Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand" potx
... identities of 97∼ 98% and amino acid sequence identities of 96∼98% with Chinese IBVs (strain A2, SH and QXIBV) Discussion One of the major problems of IBV is the frequent emergence of new variants ... S, Tamura K, Nei M MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence Detection and molecular characterization of infectious bronchitis virus 223 alignment Brief ... typing of IBV Genotyping of IBV on the basis of the S1 gene sequence, particularly the HVR region of the S1 gene, is the most Nucleotide and amino acid sequence comparison The S1 gene of the...
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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... 0.7 1.4 ± 0.2 characterization and Jannaschia sp CCS1 evaluation of HYDJs from Characterization of HYDJs To explore the possible cause of the higher specific activity for conversion of d,l-p-HPH ... positions of the Ca of Ala156 in HYDJs and the Ca of Met156 in HYDBp located in the SGL3, which leads to the increased size of the substrate binding pocket of HYDJs The side chain of Ala156 is ... performed using T-COFFEE [44] Docking simulation of substrate on the active site of HYDJs The substrate binding pockets of HYDJs and the mode of interaction between substrate and enzyme were analyzed...
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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx
... explanation for detection of an additional CRFR1e1 protein (band 10) with molecular mass of 20 kDa (Fig 2) Proteins with lower molecular mass than expected included band (39 kDa) for CRFR1f, band (34 ... of a band with 53 kDa a Ó FEBS 2004 Characterization of novel CRFR1 isoforms (Eur J Biochem 271) 2825 Fig Levels cAMP accumulation in transiently transfected COS cells with different CRFR1 isoforms ... Broad band seen in CRFR1a has an apparent molecular mass of 55–65 kDa and most likely represents glycosylated receptor We note that others have also reported detection of CRFR1 proteins with molecular...
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Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf
... reaction products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE As shown in Fig 4, 9-HODE is the main product of the hazelnut ... part of the world production adsorbed by the pastry industry Hazelnut seeds contain reasonable levels of linoleic and linolenic acid (about 10 and 0.2%, respectively, of the total fatty acids) and ... C6 and C9 aldehydes (and the corresponding alcohol) were detected in all the samples and, similarly to the pattern of LOX expression and activity, their levels were higher in stage I seed and...
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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx
... and 2,6-DMP were pH 5.6 and 3.4 UÆmg protein)1 and pH 4.6 and 2.8 UÆmg protein)1, respectively In standard assay mixtures, the velocity of ABTS oxidation was maximal at 45 °C The dependence of ... Enzyme characterization The molecular mass of purified lac1 was estimated by SDS/ PAGE to be 58 kDa and the isoelectric point of the enzyme was 3.7 In addition to ABTS, syringaldazine and 2,6-DMP ... °C Enzyme characterization The molecular mass of purified laccase was determined by SDS/PAGE (15% w/v acrylamide gels) using low molecular mass standards (Bio-Rad) The isoelectric point of the enzyme...
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Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deficient families from Southern Italy docx
... patients and seven HPA III patients 2050 Characterization and functional analysis of novel mutations Among the mutations identied in our HPA population, two (i.e p.Q301P and c.707-2delA) were novel ... varies in distinct populations and geographic areas [79] and a number of them have been analyzed and characterized in vitro [10,11] Identication of the mutations and subsequent in vitro expression ... retardation and epilepsy, as well as other neurological abnormalities [3] The severity of PAH deciency is variable and partly depends on the nature of the mutations of the PAH gene Recently, a novel...
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Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii ppt
... was 1.8°, the standard deviation of the hydrogen bond energies was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant ... in position With the exception of four a-helices and two pairs of short b-strands, the rest of the protein consists almost entirely of loops connecting the b-strands The loops extending from the ... Denaturation and refolding of reCBH and enzyme assay reCBH (1 mg) was denatured by incubation in a solution of M urea/0.1 M Tris/HCl, pH 8.0, in the presence of 100 mM dithiothreitol and mM EDTA,...
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Báo cáo khoa học: Biochemical and molecular characterization of purified chicken pancreatic phospholipase A2 docx
... 50% of its activity in the absence of NaTDC The maximal PLA2 specific activity of 400 UÆmg)1 was measured at pH 9.5 and at 37 °C in the presence of mm NaTDC and mm CaCl2 Pure ChPLA2 lost 80% of ... 4547 A Karray et al A effect of bile salts on ChPLA2 activity, the rate of hydrolysis of PC by ChPLA2 in the presence of various concentrations of bile salts, at pH 9.5 and at 37 °C, was studied ... Lys56; and aD, Ala90–Lys108); (e) two short strands 4550 of an antiparallel b-sheet (b-wing) (b2, Ser74–Ser78; and b3, Glu81–Asn85); and (f) a C-terminal loop containing a short b4-strand (His115–Asn117)...
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Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... bring to sampling and molecular study of viral crop diseases, we tested the efficacy of FTA for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene ... used to study the efficacy of FTA technology for sampling, retrieval and molecular analysis of these viruses Geminiviruses are composed of monopartite or bipartite genomes of ssDNA, 2.7–2.8 kb in ... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C...
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báo cáo hóa học:" Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc
... bring to sampling and molecular study of viral crop diseases, we tested the efficacy of FTA for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene ... used to study the efficacy of FTA technology for sampling, retrieval and molecular analysis of these viruses Geminiviruses are composed of monopartite or bipartite genomes of ssDNA, 2.7–2.8 kb in ... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C...
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Báo cáo sinh học: "Cytogenetic and molecular characterization of eight new reciprocal translocations in the pig species. Estimation of their incidence in French populations" pot
... performance of the sows sired by two of these boars only (cases and 6) The boar carrier of the 2/6 translocation sired 51 litters in two herds, with an average litter size of 8.30 The boar carrier of ... intensification of the control programmes, especially in France [10], but also by the improvement of the laboratory techniques used [27] G and/ or R banding is presently used systematically and allows the detection ... detection of rearrangements modifying very slightly the size and/ or the banding profiles of the chromosomes, as, for instance, in the case of the translocations rcp(15;17)(q24;q21) and rcp(7;8)(q24;p23)...
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IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE
... you for believing in me and letting me pursue my dreams The person I am today is because of you To my dear uncle and my aunt Khem Kandel and Laxmi Kandel: your support and friendship has been ... Crystal structure of ALDH3A1 with CB29 66 Crystal structure of ALDH3A1 with CB7 .72 Crystal structure of ALDH3A1 with CB25 81 G Expression and Activity of ALDH3A1 and ALDH1A1 in ... Characterization of CB29 binding .113 B Selectivity of CB29 for ALDH3A1 versus ALDH1A1 and ALDH2 117 C Characterization of CB7 binding 123 D Probing CB7 binding of ALDH3A1 site...
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Expressed sequence tags analysis of major allergens producing dust mites and molecular characterization of their allergens
... of a total of 120 allergic sera to DF Gal d homologue and egg white; and DF Gal d homologue and ovalbumin Figure 53: 201 Evaluation of cross-reactivity between DF Gal d homologue, ovalbumin and ... Profile of allergen collections of D farinae and B tropicalis 114 Table 13: Percentage of amino acid sequence homology between the mites FABP homologues 167 Table 14: IgE reactivity profiles of ... temperature of 23-30o C and 80-90% relative humidity The house dust mites primarily feed on skin scales and dander while the storage mites feed on plants and micro-organisms (Ho and Nadchatram, 1984; Andersen...
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Isolation and molecular characterization of genes associated with shoot regeneration of mustard (brassica juncea) in vitro
... ISOLATION AND MOLECULAR CHARACTERIZATION OF GENES ASSOCIATED WITH SHOOT REGENERATION OF MUSTARD (BRASSICA JUNCEA) IN VITRO GONG HAIBIAO (M.Sc SJTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... Ethylene and PAs are ubiquitous in plants and both are involved in the regulation of various physiological processes during plant growth and development (Yang and Hoffman, 1984; Evans and Malmberg, ... et al., 2003) and Arabidopsis thaliana (Ikeda-Iwai et al., 2003), and androgenesis in rye (Immonen and Anttila, 1999) and triticale (Immonen and Robinson, 2000) An accumulation of H2O2 in culture...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
... expressed the predicted molecular mass of protein similar to the in vitro 4095 Molecular characterization of ANKHD1 splice variant A B Fig Expression of VBARP isoforms in vitro and in vivo: (A) In ... 2B that VBARP-L and VBARP-S are coded by these specific exons VBARP-L and VBARP-S code for precursor proteins of 627 and 435 amino acids and with calculated peptide masses of 69 and 49 kDa, respectively ... Expression and biochemical characterization of VBARP The predicted sizes of the proteins encoded by VBARP-L and VBARP-S are 69 and 49 kDa, respectively To test this, C-terminal V5-tagged VBARP-L and...
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Báo cáo khoa học: "Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods" pdf
... relationship of the investigated strains The conventional and molecular methods used in the present study allowed a reliable identification and further characterization of S uberis and S parauberis and ... for the identification of streptococci of Lancefield group B Res Vet Sci 1999, 67, 159-162 Abdulmawjood, A and Lämmler, C 2000 Determination of intraspecies variations of the V2 region of the ... Smola, J and Pilich, J Diagnostic availability of the hemolytically active exosubstance of Corynebacterium pseudotuberculosis for isolation and identification of Streptococcus agalactiae and its...
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Identification and characterization of novel anticoagulants from bungarus fasciatus venom
... Discussion and Conclusion Chapter Identification and characterization of novel inhibitors on extrinsic tenase complex from Bungarus fasciatus (banded krait) Venom 3.1 Introduction 3.2 Materials and ... activity of pooled fractions Chapter Three Figure 3.1: Purification of BF-AC1 and BF-AC2 from the venom of B fasciatus Figure 3.2: ESI-MS profile of BF-AC1 (A) and BF-AC2 (B) Figure 3.3: Structural characterization ... Kang Tse Siang, Professor R Manjunatha Kini and Associate Professor Go Mei Lin for their constant encouragement and scientific input throughout my candidature Dr Kang and Prof Kini have provided...
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Identification and characterization of novel proteins from a rare australian elapid snake drysdalia coronoides
... 41 Materials and Methods 42 iii Reagents and kits; Collection of venom and venom gland; RNA isolation and cDNA synthesis; Cloning of ds cDNA; Isolation of plasmids and verification of clones; cDNA ... about 2,930 species of snakes distributed on every continent except Antarctica, islands of Ireland, Iceland and New Zealand [5, 6] They have successfully colonized various habitats and feed on small ... gene into pET-32a and pET-M; Transformation of cloning and expression host strains; Expression of protein; Purification of protein; Mass determination; Refolding of protein Results and discussion...
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Identification and characterization of novel group 5 and group 21 allergens from dust mite and ige binding epitope mapping of blo t 5
... f 21 and Der f Der f 21 and Der f in house dust samples 130 Discussion 132 135 Chapter 6: Identification and Characterization of Group and Group 21 in S medanensis and Cross Comparison of a Panel ... Effect of thermal treatment on IgE-binding activities of Blo t 92 and Blo t 21 4.2.2 Effect of thermal treatment on Blo t and Blo t 21 folding 94 4.2.3 Resistance of IgE-binding of Blo t and Blo ... alkaline and urea treatment 4.3 Discussion 102 viii 105 Chapter 5: Identification and Characterization of Der f 21: a Der f Homologue 5.1 Introduction 105 5.2 Results 107 5.2.1 Identification of a novel...
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