i cant lose you now im looking at the other half of me
Tài liệu Looking At the ADO Object Models ppt
... manner. This is another way that ADO allows developers to read outside data, such as from other applications or over the Internet. You could not do this easily with DAO. Figure A.1. The object model ... read in special tree-structured hierarchies, such as e-mail messages or file systems. You can even point the object to an URL, provided the system has set it up in a consistent manner. This is ... A.1. The object model for ActiveX Data Objects 2.7. Most of the work is done in the ADODB module when you use ADO. Whenever you use recordsets, this is also the object model to use. ...
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Tài liệu Looking at the SQL Server DMF APIs pdf
... perform the requested tasks. Following is a brief description of each of the APIs. We will be using the last two directly in the rest of this chapter. ã SQL-NS. SQL Namespace provides a ... Looking at the SQL Server DMF APIs The SQL Distributed Management Framework is a framework of objects, services, and components used to manage Microsoft SQL Server 2000. It is made up of ... of a few APIs that you can use to accomplish tasks in your applications. You can see how these APIs are used in Figure 7.3. Figure 7.3. Under the covers, the SQL-DMO objects are being used to...
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Gender-based Violence and Sexual and Reproductive Health and Rights: Looking at the Health Sector Response in the Asia-Pacific Region doc
... experiences of GBV.Recognising diversity also means looking at experiences of young women living with disabilities. While there is dearth in data in the region on GBV and young women with disabilities, ... experience in invoking human rights to ensure international commitments, such as those stated in the Convention on the Elimination of All Forms of Discrimination Against Women (CEDAW), International ... reproductive ill health, disability and death. It operates in Africa, South and South East Asia and the Pacic. SPRINT facilitates implementation of the Minimum Initial Service Package for Reproductive...
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Báo cáo y học: "Effects of Losartan on expression of connexins at the early stage of atherosclerosis in rabbits"
... of in vitro and in vivo experiments have proved that GJ may be involved in the injury or dis-ease induced activation of vessel wall cells. It is indi-cated that the expression of Cx43 in the ... fat diet group (P>0.05). The body weight at the end of expe-riment was did not change significantly compared with that at baseline (P>0.05). Furthermore, there was no significant difference ... prominent stain-ing in the latter. Panel C: After losartan treatment, Cx40 stain-ing was markedly re-duced in the neointima of arteries. The distri-bution of Cx43 was similar to that of Cx40....
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LECTURE SLIDES ON NONLINEAR PROGRAMMING BASED ON LECTURES GIVEN AT THE MASSACHUSETTS INSTITUTE OF TECHNOLOGY CAMBRIDGE, MASS DIMITRI P. BERTSEKAS
... Characterization of minima Necessary conditions Sufcient conditions Lagrange multiplier theory Sensitivity Dualityã Computation by iterative algorithms Iterative descent− Approximation methods− ... maintaining the fast convergence rate CHOICES OF STEPSIZE I ã Minimization Rule: kis such thatf(xk+ kdk) = min0f(xk+ dk).ã Limited Minimization Rule: Min over [0,s]ã Armijo ... primal-dual methods LOCAL AND GLOBAL MINIMAf(x)xStrict LocalMinimumLocal Minima Strict GlobalMinimumUnconstrained local and global minima in one dimension. LEAST-SQUARES PROBLEMSminimize...
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Tài liệu Báo cáo khoa học: Site-directed mutagenesis of a loop at the active site of E1 (a2b2) of the pyruvate dehydrogenase complex A possible common sequence motif docx
... at the C-terminal end (Fig. 2).Choice of mutations The importance of the loop region in the a-subunit of the B. stearothermophilus E1p component was highlighted by itssusceptibility to limited ... of mutations on catalytic activity The catalytic activity of E1 mutants was investigated usingthree different assays: the DCPIP assay, which measures E1activity bymonitoring the reduction ofDCPIP ... changein activity in the reductive acetylation assay and a signi cant drop in overall activity in the PDH assay when comparedwith wild-type E1. This is similar to the effects on E1 catalysis of limited...
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Tài liệu The Proof is in the Pudding - A Look at the Changing Nature of Mathematical Proof doc
... es-tablishing mathematical facts.What is interesting is that a mathematical statement of fact is a free-standing entity with intrinsic merit and value. But a proof is a device of communication. The ... relations with siblings. This is an entirely different kind of proof from the kind treated in the present book. It is in the spirit of this book in the sense that it is a “device for convincing ... any of the ideas of string theory. This isquite similar to the state in which general relativity sat for a good manyyears. String theory is a set of ideas waiting to be verified.Mathematics is...
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Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc
... evidence in support of binding of ATP to the ligand-free hGK, resulting in a change of protein conformation. The MD simulations indicatethat the binding triggers molecular motion of the flexi-ble ... steady-state kineticanalyses are also compatible with conformational con-trol of GK catalytic activity by the binding of (Mg)ATP, with possible implications for kinetic coo-perativity with respect ... acidswere in an optimal position for filling the gap in the apoen-zyme. Insertion of the missing residues was followed byenergy minimization and 3-ns MD simulation (allowingonly the inserted residues...
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Báo cáo khoa học: Site-directed mutagenesis of selected residues at the active site of aryl-alcohol oxidase, an H2O2-producing ligninolytic enzyme pot
... study, the first evidence for the involvement of some amino acidresidues in the catalytic activity of this enzymehas been obtained by site-directed mutagenesis afterin silico docking. Two histidine ... histidine residues (His502 andHis546) in the vicinity of the flavin ring were found tobe strictly required for AAO activity. One of these his-tidines is most likely involved in activation of the alco-hol ... modulating the catalyticrate of the enzyme. Finaly, L315 affects the enzyme kcat, although it is notlocated in the near vicinity of the cofactor. The present study provides the first evidence...
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Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot
... dissolvedimmediately in SDS and 2-mercaptoethanol with proteaseand protein phosphatase inhibitors, frozen within 10 s, andthawed in boiling water to minimize postincubation signal-ling modifications in the ... ter-minally differentiated cells that do not divide further,insulin has the potential for genomic control via amitogenic signalling pathway. This may also be medi-ated by IRS; insulin activation ... increasedphosphorylation of all three proteins (Fig. 3). Thispattern was not significantly changed by overnightincubation of the cells (Fig. 3). Maximal insulin-stimulated increase in tyrosine phosphorylation...
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Báo cáo khoa học: Do N-terminal nucleophile hydrolases indeed have a single amino acid catalytic center? Supporting amino acid residues at the active site of penicillin G acylase pptx
... and initial velocities were calculated, taking intoaccount both the consumption of the substrate and the lib-eration of the arylamine with the time. The kinetic experi-ments with each substrate ... feature of the PGA catalytic reaction. It is probably a cumulativeresult of intrinsic amino acid interactions in the activesite of the E. coli enzyme that differentially (de)stabi-lize the structures ... because of the substitution of the oxyanion hole with twowater molecules. The effective stabilization of the oxy-anion is the main driving force of the first step of acyl-ation, and dominates all other...
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Báo cáo khoa học: MPP3 is recruited to the MPP5 protein scaffold at the retinal outer limiting membrane ppt
... determine the vectors with correctinsert orientation. This resulted in insertion of the epitope at the very amino terminal end.Protein purification and antibody productionFor the purification of ... protein motifs. Mutations have beenidentified in the CRB1 gene in individuals with Lebercongenital amaurosis, retinitis pigmentosa (RP) type12 with preservation of para-arteriolar retinal pigmentepithelium ... 3), Mint1, and actin-binding LIM pro-tein. Some of the MAGUKs identified bind directlyto the channel, like DLG1 and Veli [35,36] whileothers are recruited via binding to another MAG-UK, like...
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Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx
... as described [14] prior to initiation of the phosphorylation reaction in the presence of inhibitoryH4biopterin.Limited proteolysis by trypsinLimited tryptic proteolysis of tetrameric wt-hPAH ... side-chain of Ile25.Discussion The catalytic activity of PAH is regulated by four mainmechanisms, i. e. by substrate (L-Phe) activation, biopterincofactor (H4biopterin/H2biopterin) inhibition, ... and Ile25 (Fig. 4), which results inan inhibition of Ser16 phosphorylation similar to that of H4biopterin (Fig. 3B) with a half- maximum inhibition at aconcentration < 0.1 lM. This value...
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Acne Symposium at the World Congress of Dermatology Pari doc
... sys-temic administration in 10 patients with inflammatoryacne. Clinical evaluation of these patients indicated anapproximately 60% decrease in the acne severity indexwithin 3 weeks of the initiation ... assessed the action of IL-1· in the microdissected human pilosebaceous infundibulum prep-arations in vitro and found an IL-1·-specific induction of hypercornification of the infundibulum similar ... migration of polymorphonuclear leu-kocytes stimulated by LTB4. Topical treatment with iso-tretinoin resulted in a marked and statistically significantinhibition of the LTB4-induced migration...
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