h— endocrine regulation of postprandial gallbladder motility

Regulation of na+ h+ exchanger 1 (NHE1) stability in PTEN mouse embryonic fibroblasts

Ngày tải lên : 10/09/2015, 09:29

... Conversely, downregulation of Akt1 and Akt2 has been shown to promote migration and invasion in PC3 prostate cancer, suggestive of the role of isoform-specific Akt in the regulation of cell motility ... function of NHE1 1.1.3.A pH and Cell Volume Regulation One of the most well-known functions of NHE1 is the regulation of intracellular pH (pHi) and cell volume The transmembrane domain of NHE1 ... likely the role of CHPs in the regulation of stability of NHE1 may vary depending on the cell or tissue type The rate of synthesis and degradation dictates the intracellular level of a protein Likewise,...

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Regulation of na+ h+ exchanger 1 (NHE 1) gene expression by mild oxidative stress

Ngày tải lên : 14/09/2015, 08:50

... 53 1.4.4 Regulation of activity and expression of NHE‐1 54 1.4.4 A 1.4.4 B 1.5 Regulation of NHE-1 activity 54 Transcriptional regulation of NHE-1 expression .58 AIM OF STUDY ... responsible to induce the inhibition of NHE‐1 promoter by H2O2 207 3.8 PHYSIOLOGICAL IMPORTANCE OF NHE-1 GENE REGULATION .212 3.8.1 Effect of mild oxidative stress on NHE‐1: The regulation of intracellular pH and cell cycle ... exposure of cells to non-toxic doses of H2O2 led to the inhibition of the ubiquituously expressed regulator of intracellular pH, NHE-1 Hence, the mechanism of NHE-1 gene repression upon exposure of...

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Reactive oxygen species mediated regulation of the na+ h+ exchanger, NHE 1 gene expression a new mechanism for tumor cells resistance to apoptotic cell death

Ngày tải lên : 16/09/2015, 08:31

... intracellular milieu 102 IV.3 Alkaline pHi and role of NHE-1 103 IV.4 Regulation of NHE-1 105 IV.5 Rationale of our study 105 IV.6 Regulation of NHE-1 gene expression regulates cells’ response ... transport H+ out of the cell in exchange for Na+ with a stoichiometry of 1:1 In mammalian cells, the NHE family consists of nine isoforms, NHE-1 to NHE-9 Whereas most of the isoforms are restricted ... other types of chemotherapy Major mode of action of taxanes is thought to be their ability to bind to tubulin leading to G2/M arrest of the cell and subsequently apoptosis Down -regulation of NHE1...

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fr h 109 abstract of trim calculation

Ngày tải lên : 17/09/2013, 21:00

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T H E BENEFIT OF ASKING THE RIGHT QUESTIONS

Ngày tải lên : 25/10/2013, 15:20

... explanations and descriptions of human behavior, we prefer that explanations or descriptions of the rate of abortion, the frequency of unemployment, or the causes of child abuse be consistent ... examination of those beliefs will sometimes reinforce our original commitment to them A long time ago, John Stuart Mill warned us of the emptiness of a set of opinions accumulated without the help of ... a moment of anger only because a gun is available In fact, every study of homicide shows the overwhelming majority of murderers are career criminals, people with lifelong histories of violence...

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Regulation of S Phase

Ngày tải lên : 25/10/2013, 21:20

... initiation (Fig 1) The chromatin loading of MCM2-7 depends on the loading of Cdt1 and Cdc6, which in turn depend on the loading of ORC This step- Fig Regulation of the pre-RC The pre-RC functions to ... is also co-dependent with Cdc45, Dpb11, and Sld3; binding of any of Regulation of S Phase 47 these proteins depended on the presence of the others (Kubota et al., 2003; Takayama et al., 2003) ... characterization of Xlinked mutants of Drosophila melanogaster which are sensitive to mutagens Genetics 84:485–506 Brown GW, Kelly TJ (1999) Cell cycle regulation of Dfp1, an activator of the Hsk1...

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Regulation of navigation and vessel-source pollution in the Northern Sea Route - Article 234 and state practice

Ngày tải lên : 01/11/2013, 09:20

... accompaniment of the CCGS John A 40 41 42 43 See Arts and 10 of the NSR Regulations; Arts 14 and 15 of the Economic Zone Edict; Arts and 15 of the Environmental Edict; and Art 11 of the Decree of the ... the Decree of the Council of Ministers of the USSR, June 1990, ‘On Measures of Securing the Implementation of the Edict of the Presidium of the USSR Supreme Soviet of 26 November 1984 “On Intensifying ... the NSR Regulations, as stated in Article (quoted above), include regulation of navigation, on the basis of non-discrimination These regulations are to apply to all vessels, regardless of nationality,84...

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Tài liệu Báo cáo khoa học: miRNAs and regulation of cell signaling pptx

Ngày tải lên : 14/02/2014, 19:20

... repression of their target genes Indeed, several examples of feedback regulation that involve miRNAs have been reported Below, we attempt to summarize our recent understanding of feedback regulation of ... ﬁnding that TPAinduced upregulation of miR-34a depends on the activation of the ERK signal cascade and that miR-34a downregulates MEK1, which is one of the main regulators of ERK signaling, indicates ... expression of estrogen receptor-a (ERa) is autoregulated by miR-18a, -19b and -20b, which in turn are upregulated by the activation of ERa This mechanism of regulation provides a wide range of coordinated...

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Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

Ngày tải lên : 14/02/2014, 22:20

... direct dephosphorylation of Ser112 and negative regulation of the ERK pathway via p38 MAPK, both of which lead to impaired phosphorylation of Ser112 [70] After dephosphorylation of Ser112, Ser136 becomes ... translocation of Bax and Bad to mitochondria independently of caspase activation [77,87] Phosphorylation of caspases Phosphorylation of caspases switches the cellular apoptotic signal on and off Caspase ... regulating the activity of an isoform of PP1, PP1a [3] Similarly, activation of caspase-8 and -3 is regulated through their phosphorylation and dephosphorylation Phosphorylation of caspase-8 at Tyr397...

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Tài liệu Regulation of cancer cell metabolism docx

Ngày tải lên : 15/02/2014, 04:20

... splicing The inclusion of exon in the PK mrnA leads to translation of the PKM1 isoform, whereas inclusion of exon 10 produces PKM2 (ReF 71) MyC upregulates the expression of heterogeneous nuclear ... required for the assembly of the cytochrome c oxidase complex of the electron transport chain61 Thus, the loss of p53 might also be a major force behind the acquisition of the glycolytic phenotype ... Publishers Limited All rights reserved REVIEWS of genes that increase glucose metabolism and reduce mitochondrial respiration One of these genes encodes an isoform of PDK (PDK4) that has the same function...

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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

Ngày tải lên : 16/02/2014, 09:20

... general context of a promoter appears to be relevant because inversion of the site in the human Cdc25C promoter resulted in a deregulation of cell cycle-dependent transcription Deregulation is ... promoters, mutation of the CHR leads to a more pronounced deregulation than the alteration of the CDE [40] Another gene tested for its cell cycle regulation is the cellular inhibitor of apoptosis protein ... investigating downregulation of the cyclin B2 promoter is that after deletion or mutation of constitutively activating sites, such as the destruction of CCAAT-boxes, small levels of reporter activity...

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Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

Ngày tải lên : 16/02/2014, 09:20

... genetic diseases [47] Most analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target gene expression, have ... treatment of the chick face with RA, and this is accompanied by an increase in apoptosis [73] The authors of this study ascribe the regulation of outgrowth of limb buds and patterning of the digits ... development Analyses of AP-2a-null mice have demonstrated that AP-2a is a fundamental regulator of mammalian craniofacial development AP-2a knockout mice die perinatally with craniofacial defects,...

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Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

Ngày tải lên : 18/02/2014, 04:20

... strict regulation becomes necessary for the correct expression of HSF2 target genes Indeed, when miR18-mediated regulation of HSF2 was disrupted in male germ cells in vivo, expression of HSF2 ... modiﬁcations of HSFs in different tissues and organs during development of an organism or differentiation of certain cell types This lack of knowledge severely hampers the understanding of the physiological ... HSEs, the Regulation of HSFs HSFs regulate different types of target genes that are involved in a broad range of cellular processes Therefore, the expression and activity of HSFs need to be under...

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Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt

Ngày tải lên : 18/02/2014, 08:20

... the regulation of miR-27 expression Our data suggest that the miR-27 gene family is potentially an important class of negative regulators of adipogenesis and may play a role in the regulation of ... relevant role of miR-27 in the regulation of adipose tissue functions in vivo, we examined the expression of miR-27 in the genetically obese ob ⁄ ob mice The 2352 expression levels of both miR-27a ... suggesting that downregulation of miR-27 is not sufﬁcient to promote adipogenesis We performed a time course study to gain further insights into the role of miR-27 during different stages of adipogenesis...

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Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

Ngày tải lên : 18/02/2014, 11:20

... molecular weights of 161.8, 28.2 and 13.2 kDa, respectively Regulation of cathepsin B activity The formation of the cathepsin B ⁄ cystatin C complex was seen as a shift of the elution peak of cathepsin ... the presence of various concentrations of 2A2 mAb The binding of 2A2 mAb was followed by native gel electrophoresis Increasing the concentration of 2A2 mAb at a ﬁxed molar ratio of cathepsin ... the conformational changes of the active site of cathepsin B, the results obtained in the present study provide a speciﬁc mechanism for the regulation of the activity of cathepsin B, which can...

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Tài liệu Báo cáo khoa học: Mixed lineage leukemia histone methylases play critical roles in estrogen-mediated regulation of HOXC13 docx

Ngày tải lên : 18/02/2014, 14:20

... level of MLL1 mRNA Interestingly, upon downregulation of MLL1, E2-mediated upregulation of HOXC13 was slightly decreased (Fig 4A, lane 3) Similar results were observed for MLL2 and MLL4 downregulation ... ERE2, ERE3 and ERE4 of the HOXC13 promoter As seen in Fig 5A, no significant binding of actin was observed in any of the EREs, irrespective of the absence and presence of E2 Binding of ERa and ERb ... amount of binding of MLL1 was observed in ERE3, no significant E2-dependent binding of any of the MLLs was observed in ERE3 and ERE4 (Fig 5A, lanes 5–8) Significant amounts of constitutive binding of...

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Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

Ngày tải lên : 18/02/2014, 16:20

... with dCTP (grey) and dTTP (yellow, cyan and magenta) (B) Stereoview of a superposition of one of the subunits of the E138A variant of E coli dCTP deaminase in complex with dTTP (yellow; chain B), ... Mutational analysis of amino acid residues involved in dTTP regulation of dCTP deaminase The design of the mutant enzymes H121A and V122G was inspired by the results from analysis of crystal structures ... roles of His121 and Val122 in catalysis and regulation of dCTP deaminase Removal of the imidazole ring in H121A was anticipated to relieve or reduce inhibition by dTTP by preventing expulsion of...

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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx

Ngày tải lên : 18/02/2014, 16:20

... swelling-induced inhibition of proteolysis in perfused rat liver J Nutr 126, 395–402 Haussinger D & Schliess F (1999) Osmotic induction of ¨ signaling cascades: role in regulation of cell function Biochem ... activation of the p38-type MAPK and thereby inhibition of 5800 autophagic proteolysis [14] Thus, integrin-dependent cell volume sensing and signaling integrates into the overall context of insulin ... of 3T3 ﬁbroblasts to growth factors [17,18] Activation of Na+ ⁄ K+ ⁄ 2Cl– cotransport via NKCC1 was shown to be essential for cell cycle progression in 3T3 ﬁbroblasts [17,19] The importance of...

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Tài liệu Báo cáo khoa học: Down-regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism docx

Ngày tải lên : 18/02/2014, 16:20

... malabsorption of folate was observed over the entire course of ethanol treatment of months Determination of the kinetic constants of [3H]folic acid uptake in BBMVs V(pmol/30 sec/mg protein) The effect of ... primary absorptive site of folic acid, i.e the small intestine Expression of the RFC protein in BBM of the intestine The effect of chronic alcoholism on the level of expression of the RFC protein ... expression of a major folate transporter, RFC, was studied The investigation of the regulation of folate transport via RFC expression in absorptive epithelia may aid in the development of future...

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Tài liệu Báo cáo khoa học: Redox regulation of dimerization of the receptor proteintyrosine phosphatases RPTPa, LAR, RPTPl and CD45 pdf

Ngày tải lên : 18/02/2014, 17:20

... role of oxidation in dimerization of a panel of four different RPTPs We compared four RPTPs from different subtypes, i.e RPTPa, RPTPl, CD45 and LAR We found that the cytoplasmic domains of these ... ectodomains of LAR and RPTPl may drive homophilic interactions [35–38] To remove contributions of the ectodomains to dimerization, we generated chimeras consisting of the extracellular domain of RPTPa ... consequence of 2600 oxidation of the catalytic site cysteines Taken together, these limited tryptic proteolysis suggest that oxidation induced a change in conformation of the intracellular domain of this...

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