human embryonic stem cell bank implication of human leukocyte antigens and abo blood group antigens for cell transplantation
Myocardial protection and therapeutic angiogenesis using peptide and embryonic stem cell transplantation
... be achieved by transplantation of exogenous stem cells and differentiated cells and also by enhancing the mobilization of endogenous stem cells These cells have natural capacity of participating ... Peripheral blood- derived Umbilical cord-derived Embryonic stem cell- derived 44 45 46 1.7 Embryonic stem cells- a new era in therapeutic angiogenesis 1.7.1 In vitro differentiation of human embryonic stem ... angiogenesis and vasculogenesis: Cellular approach Adult mesenchymal stem cells Smooth muscle cells Hematopoietic stem cells and progenitor cells Endothelial progenitor and endothelial cells Bone...
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Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx
... sampling and processing, we generated a cell bank of cultured mES cells For years, the importance of mES cells to biology and medicine has been attributed both to their ability to proliferate for ... mES cell bank for the overexpression of 32 distinct genes, we also developed a standardized approach for the generation of mES clones to be added to this cell bank This opens the possibility of ... for technical assistance in the generation of mES inducible clones We thank Dr Lucia Perone and the Cell Culture and Cytogenetics Core of Tigem for the karyotyping of mouse ES clones of the cell...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
... pluripotent stem cells However, less is known about the conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines ... Cokus and Weihong Yan for the data visualization on genome browser, Amander Clark for providing embryonic stem cell DNA (HSF1) and discussion P-YC is funded by Eli and Edythe Broad Center of Regenerative ... Innovation Award from the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA (to SEJ and MP), and by the Department of Energy Office of Science (DE-FC02-02ER63421...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells ... upon to LMO2, of involved sets of cell breast (Affyme- Authors' contributions SJL and QF designed and performed the cell culture, differentiation and RNA isolation JAH and JDH performed the microarray ... H, Baker SC, et al.: Genome wide profiling of human embryonic stem cells (hESCs), their derivatives and embryonal carcinoma cells to develop base profiles of U.S Federal government approved hESC...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... Mouse embryonic fibroblast xiv Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, and ... Feeder-free culture of human pluripotent stem cells 28 3.2 Expansion and mitotic inactivation of MEF cells 28 3.3 Preparation of MEF-conditioned medium 29 iii 3.4 Culture of PA6 mouse skull ... expressed in pluripotent stem cells, and is required for self-renewal of undifferentiated cells Zfp206 physically associates with both Oct4 and Sox2, and genome-wide mapping of Zfp206 binding sites...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... important in human embryonic and neuronal developmental networks The enriched and highly homogenous cultures of neural progenitors and neurons derived from hESCs provided an ideal source of cells for ... hESCs and NPCs (Figure 5.1) In addition, the expression profiles of these lncRNAs mirror that of known drivers of neuronal differentiation (Figures 5.2E-F), and were therefore indicative of important ... specific to pluripotent cells Thus, the expression levels of the 16 lncRNAs in undifferentiated human pluripotent stem cells and a panel of somatic tissues were quantified Three of the pluripotency...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... GenBank IDs: AK056164, AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and ... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
... M., and Huttner, W.B (2005) The cell biology of neurogenesis Nat Rev Mol Cell Biol 6, 777-788 Gregg, C., and Weiss, S (2003) Generation of functional radial glial cells by embryonic and adult forebrain ... Reiter, J.F., and Stanford, W.L (2010) Polycomb-like associates with PRC2 and regulates transcriptional networks during mouse embryonic stem cell selfrenewal and differentiation Cell Stem Cell 6, 153-166 ... Dopaminergic differentiation of human embryonic stem cells Stem Cells 22, 925-940 Zhang, Y., Liu, X.S., Liu, Q.R., and Wei, L (2006) Genome-wide in silico identification and analysis of cis natural antisense...
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A human embryonic stem cell based model of trophoblast formation
... Australia) A Human Embryonic Stem Cell based Model of Trophoblast Formation (manuscript in preparation, 2009) Inhibition of methylation releases the developmental plasticity of mouse embryonic stem cells ... project and helped me to accomplish it From our lab, Jameelah Sheik Mohamed and Sun Lili for the human embryonic stem cell (hESC) culture work and Woon Chow Thai for the microarray experiments and ... A Human Embryonic Stem Cell based Model of Trophoblast Formation LUO WENLONG (BSc., China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES...
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Guidelines for Human Embryonic Stem Cell Research potx
... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... types of cells It is believed that stem cells also form reservoirs of repair cells to replace cells and tissues that degenerate over the life span of the organism The dual capacity of stem cells for ... standardization and for validation of results The safe handling and storage of blastocysts and stem cell material and conditions for transfer of such material among laboratories Prerequisites to hES cell research...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
... use of adult and embryonic stem cells to study human hematopoiesis Curr Opin Biotechnol 2005, 16:510-515 Anderson JS, Bandi S, Kaufman DS, Akkina R: Derivation of normal macrophages from human embryonic ... generation of an unlimited supply of CD34+ cells thus paving the way for utilization of these cells for hematopoietic cell therapy [9] Recently we demonstrated derivation of phenotypically and functionally ... hES-CD34+ cells can give rise to normal DCs which are capable of supporting HIV-1 infection Results Hematopoietic differentiation of human ES cells and derivation of dendritic cells hES cell line...
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Báo cáo khoa học: " Challenging complications of treatment – human herpes virus 6 encephalitis and pneumonitis in a patient undergoing autologous stem cell transplantation for relapsed Hodgkin''''s disease: a case report" doc
... allogeneic stem cell transplantation, severe disease is rare in patients after autologous stem cell transplantation Nevertheless, in patients with severe complications of infections after autologous stem ... Authors' information G.J and B.M are attending physicians in the department of hematology and oncology of the University of Ulm P.S is attending physician in the department of Diagnostic and Interventional ... had received four cycles of ABV and involved field radiation Seven months later the lymphoma relapsed and two cycles of DexaBEAM with stem cell harvest were applied We performed high-dose chemotherapy...
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Báo cáo khoa học: " Challenging complications of treatment – human herpes virus 6 encephalitis and pneumonitis in a patient undergoing autologous stem cell transplantation for relapsed Hodgkin''''s disease: a case report" ppsx
... allogeneic stem cell transplantation, severe disease is rare in patients after autologous stem cell transplantation Nevertheless, in patients with severe complications of infections after autologous stem ... Authors' information G.J and B.M are attending physicians in the department of hematology and oncology of the University of Ulm P.S is attending physician in the department of Diagnostic and Interventional ... had received four cycles of ABV and involved field radiation Seven months later the lymphoma relapsed and two cycles of DexaBEAM with stem cell harvest were applied We performed high-dose chemotherapy...
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Human embryonic stem cell derivatives as cancer therapeutics
... factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic stem cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic ... derivatives are used for cancer therapy VIII LIST OF PUBLICATIONS Comparative Study of Effects of Neural Stem Cells and Mesenchymal Stem Cells on Differentiation, Maturation and Function of Human Monocyte-Derived ... 2001) - Used for evaluation of the effects of adult stem cells on differentiation of DCs from monocytes - Used for evaluation of the effects of adult stem cells on differentiation of LPS-induced...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage ... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... universal stem cell marker and may be used to identify and isolate NSCs Gain -of- function and loss -of- function studies in Xenopus provide the first few evidences of the role of Sox2 in maintaining ‘stemness’...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
... and MEF-lysed matrix need to be replaced by transplant-friendly and costeffective culture systems In view of the imperfections of existing culture systems, an optimal system for the culture and ... Summary of hESC culture systems 14 1.5 Hypothesis A human ECM can be bio-assembled in vitro, decellularized and subsequently applied for the sustained growth and maintenance of pluripotency of hESCs ... sites for integrins, also serves for cell attachment and the control of cellular behavior, such as cell migration and differentiation [62, 61] 41 LTBP-1 was found to be present in each of the...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
... Fibronectin*† 100.00% 21 14.60% Isoform of Fibronectin*† 100.00% 15 8.51% 240832 Isoform of Tenascin*† 100.00% 5.77% 100.00% 4.54% 77311 Isoform of Transglutaminase Isoform 2C2 of Collagen alpha-2(VI) ... 53635 152453 Isoform of Myosin9*† Isoform of FilaminA* 100.00% 2.81% 100.00% 1.10% Isoform of 100.00% 3.33% 47 IPI00220967, IPI00744135, IPI00856098 IPI00009865 58811 IPI00220327 Extracellular Matrix ... Fibrillin-1* Isoform of Collagen alpha-1(XII) chain Isoform of Collagen alpha-3(VI) chain*† 100.00% 4.10% 100.00% 8.57% 100.00% 100.00% 100.00% 23 8.17% 1.25% 11.80% 100.00% 15 7.84% 262599 Isoform of Fibronectin*†...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
... the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for the hESCs, since DCN and BGN both bind active TGFB, and could lead to a decrease ... been optimized for Matrigel, the standard feeder-free culture system Subculture was done every 5-7 days depending on the confluency of the hESCs In this way, hESCs were maintained for up to 20 ... representative cultures of hESCs from each matrix condition were sacrificed for cell counting using a haemacytometer Population doublings from each passage were calculated and the data plotted for comparison...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
... expression 77.5% of hESCs on Matrigel were positive for SSEA3, with 72.2% for hESCs on DxSDOC and 70.6% for hESCs on DxSDOCDOC 78.9% of hESCs on Matrigel were positive for SSEA-4, and 85% for hESCs ... 88.9% and 88.7% for hESCs on DxSDOC and DxSDOCDOC respectively Similarly, 85.7% of hESCs on Matrigel expressed SSEA-4, with 84.2% and 88.1% for DxSDOC and DxSDOCDOC hESCs respectively Only 34.6% of ... sample, triplicates of 10,000 labeled cells were used for each marker An average of the percentage of positively-labeled cells of each triplicates was plotted, together with the standard deviation...
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