human derived liver cell line for the detection of protective effect of dietary antioxidants against food mutagens
Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
... sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 is negative for HR HPV; Cutoff ratio greater than 1.0 positive for HR HPV The cut off ratio at 1.0 ... find out the positivity rate between HPV detection and oral lesions We did not detect any type of low risk humanpapilloma virus in these lesions The HC-II assay used for the detection of the presence ... was to compare the efficacy of HC-II assay and PCR for the detection of specific HPV type (HPV 16 E6) in OSMF and OSCC cases as well as find out the prevalence rate of the HR-HPV in these lesions...
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Báo cáo hóa học: " Inhibition of histone deacetylation in 293GPG packaging cell line improves the production of self-inactivating MLV-derived retroviral vectors" pdf
... 97% of the target cells were positive for the EGFP reporter respectively On the other hand, only 4.5% of A549 cells http://www.virologyj.com/content/3/1/27 were transduced with an equal volume of ... revised the manuscript PB contributed to the conception of the designs in the study, acquisition of funding, and revised the manuscript JG had substantial contribution to the conception of the study, ... transfected packaging cells and to minimize the risk of rescue of the SIN deletion in the 3'LTR We expected interferences between the internal CMVIE and the upstream 5'CMV in the SIN retrovector...
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Báo cáo hóa học: "Biocompatible micro-sized cell culture chamber for the detection of nanoparticle-induced IL8 promoter activity on a small cell population" pot
... between the individual cell responses Analysis of the biocompatibility of the MCC Figure The miniaturized cell culture chamber (a) Work flow of the fabrication (b) Design of the MCC The MCC contains ... in the corner areas of the microcavity (Figure 3f) The performed studies verify the biocompatibility of the Si3N4 membrane and the suitability of the microcavity for in vitro studies A549 cells ... sensitivity, the use of the miniaturized system for the detection of threshold-dependent effects was tested The comparison of the data obtained using a 96-well microplate and the developed micro-sized cell...
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Constructive role of internal noise for the detection of weak signal in cell system
... among the noise, the noise-induced oscillation, and the signal so as to intensively enhance the ability of the cell system to detect the weak signal Fig.1 shows the time series of the variable x for ... resonance among the noise, the noise-induced oscillation, and the signal can intensively enhance the ability of the system in detection of the weak signal, especially when the frequency of the signal ... around that of the intrinsic oscillation of the system And, because the frequency of the intrinsic oscillation can be adjusted by the internal or external modulations, the system can effectively...
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Báo cáo y học: "Comparison of a Two-Lead, Computerized, Resting ECG Signal Analysis Device, the MultiFunction-CardioGramsm or MCG (a.k.a. 3DMP), to Quantitative Coronary Angiography for the Detection of Relevant Coronary Artery Stenosis (70%)
... large inventory of normalized mathematical indexes of abnormality It is the pattern of these mathematical indexes of abnormality, obtained from analysis of multiple cardiac cycles of the resting ... a 25-mm section of waveform in any 5.12-second segment of the ECG data, the waveform strays from the baseline by ≥3 mm, • a radical deviation away from the baseline angle 146 of at least 80° ... centers included in the meta-analysis The boundaries of the box are Tukey’s hinges The median is identified by the line inside the box The length of the box is the interquartile range (IQR) computed...
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Genotoxicity of 255 chemicals in the Salmonella microsome test (Ames test) and 8-hydroxyguanine (8-OH-Gua) assay for the detection of carcinogens
... supported by the research project The research on the development of the total evaluation technique for the hazardous impacts by the chemical substances towards human and ecology (the project ... ultimate forms in the presence of a mammalian metabolic activation system, such as human liver S9 fraction as well as rat liver S9 fraction This result suggests that human liver S9 fraction in the ... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts...
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The Use of Plant Cell Biotechnology for the Production of Phytochemicals
... Consequently, the development of an information base on genetic, cellular, and molecular levels is now a prerequisite for the use of plants or plant cell cultures for biotechnological applications for the ... relatively easy to perform One of the major applications of genome sequencing of plants is functional genomics In simple words, an understanding of the function of genes and other parts of the genome is ... All of these data are then used in the second step to reconstruct the biochemical reaction networks that underlie the cellular function of interest When such data are put into the format of a...
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Critical evaluation of diagnostic aids for the detection of oral cancer docx
... respectively One of the weaknesses of this study is the small sample size In addition, the majority of the lesions examined appear to be of the Class I type, rather than Class II lesions for which the technology ... disease in which the patient’s T cells attack the basal cells of the oral mucosa As such, the cytologic and morphologic changes observed in cell dissociates derived from a lesion of lichen planus ... imaging involves the exposure of tissue to a rather specific wavelength of light, which results in the autofluorescence of cellular fluorophores after excitation The presence of cellular alterations...
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INTERNATIONAL STANDARD Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Salmonella spp docx
... drops of the creatine solution, three drops of the ethanolic solution of 1-naphthol and then two drops of the potassium hydroxide solution; shake after the addition of each reagent The formation of ... preparations of the initial suspension for certain foodstuffs NOTE The following specific preparations concern only the case of Salmonella Specific preparations applicable for the determination of any ... incubation for 24 h ± h, examine the plates (9.4.3) for the presence of typical colonies of Salmonella and atypical colonies that may be Salmonella (see Note) Mark their position on the bottom of the...
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ag doped wo3-based powder sensor for the detection of no gas in air
... the concentration of NO and NO2 gases because of the low concentration, one of the roles of the Ag is apparent to provide surface for the conversion of NO to NO2 according to the literature [18] ... one of the roles of promoter may be to provide a surface for the catalytic conversion of NO to NO2 that are responsible for the high sensitivity associated with the promotion However, whether ... and was then diffracted onto the detector The detector collected data for all angles in the range 4±648, 2y, simultaneously Fig gives the diffraction spectra of the WO3 before and after the NO...
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Báo cáo khoa học: The structural comparison of the bacterial PepX and human DPP-IV reveals sites for the design of inhibitors of PepX activity pot
... into the active site of the human DPP-IV; 3, docked into the active site of the crystalline structure of PepX from L lactis; and 4, docked into the active site of the modeled structure of PepX ... lactis The results are presented in Table and Fig 4A For the cluster of lowest energy, both the position and the conformation of the ligand are close to those observed in the 3D structure of the ... of the ligand and the NH2 of Asn470 (equivalent to Asn710 in DPP-IV) Another potential bond can be proposed between the OH atom of Tyr380 and the carbonyl group of the ligand Finally, the valine...
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Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx
... to those in the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses ... with the human gene (unpublished results) Using PCR primers derived from consensus sequences of the human and monkey gene, we determined the sequence of the entire OSM coding sequence of the M ... genomes/106 cells which is the number of viral copies of RRV in µg of DNA from the MmuA01111 spleen To ensure that the RV2 assay does not detect RV1 viruses, the assay was performed using DNA from the human...
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Báo cáo hóa học: " Analysis of in vitro replicated human hepatitis C virus (HCV) for the determination of genotypes and quasispecies" docx
... into the Huh7 cell line or variants thereof Although a number of studies have been done in non -human primates, the relationship of Replicon systems to human diseases is not known yet As Huh7 cells ... induction of apoptosis [13], it is likely that the multiplication of Replicons in Huh7 derived cells may be due to the unusual properties of these cells rather than a unique capability of Replicons ... sequenced The dates of transmission indicate the date that HCV was added to the cells For the T1 through T7 isolates, the exact date of transmission sometimes wasn't known, but the first date of isolation...
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báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot
... to those in the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses ... with the human gene (unpublished results) Using PCR primers derived from consensus sequences of the human and monkey gene, we determined the sequence of the entire OSM coding sequence of the M ... genomes/106 cells which is the number of viral copies of RRV in µg of DNA from the MmuA01111 spleen To ensure that the RV2 assay does not detect RV1 viruses, the assay was performed using DNA from the human...
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báo cáo hóa học: " Glrt-Based Array Receivers for The Detection of a Known Signal with Unknown Parameters Corrupted by " docx
... (12) then corresponds to the correlation of the real part, z1c(nT), of the SMF’s output, y1c(nT), with the known real symbols, an, over the known signal duration B Performance The performance of ... detectors as the phase diversity between the sources increases We still note the best performance of the completely informed detectors (C1 and O1) and the lowest performance of the less informed detectors ... The protection of the desired signal and the rejection of the two interferences then require + + = degrees of freedom, which in fact corresponds to the number of degrees of freedom, 2N = 4, effectively...
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Báo cáo hóa học: " A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA" ppt
... cutoff value in succedent detection was determined as a positive result The ten HPV-16-positive samples were used to validate our hybridization assay on the basis of the cutoff value Detection of ... protection The resultant mixture was stirred for about 20 and then boiled at 100°C The reflux time to get the CdTe QDs was h X-Ray diffraction (XRD) was used to confirm the crystalline phase of QDs ... hybridization The detection procedure was described in detail in the next section (Figure 1) The cutoff value was defined as the mean fluorescence intensity of HPV-16-negative human DNA samples...
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báo cáo hóa học:" Research Article Linear Classifier with Reject Option for the Detection of Vocal Fold Paralysis and Vocal Fold Edema" doc
... is derived The optimal operating point of the linear classifier is specified with and without reject option The contribution of this paper is in the assessment of the impact of reject option in the ... hull of the experimental ROC curve of the linear classifier without reject option (solid line) with the level lines of slope α (dashed lines) overlaid (b) Zoom in (a): the arrow points to the optimal ... hull of the experimental ROC curve of the linear classifier without reject option (solid line) with the level lines of slope α (dashed lines) overlaid (b) Zoom in (a), the arrow points to the optimal...
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Báo cáo hóa học: " Quinoline Group Modified Carbon Nanotubes for the Detection of Zinc Ions" ppt
... device in intracellular environment for the detection of Zn(II) References ð1Þ Wherein, A is the value of the fluorescence intensity, C is the concentration of Zn(II), and the range of C is from ... selectivity when they are used just for Zn(II) detection On the other hand, the fluorescence enhancement of MWNTs-GNQ is only for Zn(II) As the use of organic–inorganic hybrid materials for bio-application ... happened in the temperature range from 200 °C to 450 °C due to the degradation of the GNQ grafted to the MWNTs The content of the GNQ grafted to the MWNTs is about 12 wt%, which is similar to the calculation...
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Báo cáo hóa học: " Research Article New Structured Illumination Technique for the Inspection of High-Reflective Surfaces: Application for the Detection of Structural Defects" doc
... methods: the center -of- mass and the Blais-and-Rioux operators The first uses the distribution of the grey level to retrieve the positions of the maxima, whereas the second applies a local linear ... of a widely used real-time industrial application The core component of the algorithm is the selection and the extraction of the features best describing the image contents The first step of the ... illuminations must be used for covering the complete circumference of a metallic cylinder The number NC of needed cameras depends not only on the diameter of the object DO , the width of the ray aperture’s...
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Báo cáo khoa học: "Comparison of Diagnostic Methods for the Detection of Parasites in Fish" pdf
... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the ... normal PCR reaction of 50 µl volume (25µl of master mix, Comparison of Diagnostic Methods for the Detection of Parasites in Fish 2µl of each primer, or µl of template and 18 or 20 µl of water) - Polymerase ... Comparison of Diagnostic Methods for the Detection of Parasites in Fish al., 1998; Anderson, 1999) Our primer sets were designed for identification of different flukes and they were useful for detection...
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