hsod catalase hcat and glutathione peroxidase hgpx

Báo cáo Y học: Two GPX-like proteins from Lycopersicon esculentum and Helianthus annuus are antioxidant enzymes with phospholipid hydroperoxide glutathione peroxidase and thioredoxin peroxidase activities pptx

Ngày tải lên : 08/03/2014, 22:20

... GPXle1 and GPXha2 The glutathione peroxidase activities of GPXle1 and GPXha2 towards several physiological and nonphysiological hydroperoxides were monitored in the presence of glutathione and glutathione ... F.F (1994) The human glutathione peroxidase genes GPX2, GPX3 and GPX4 map to chromosome 14, and 19, respectively Cytogenet Cell Genet 66, 96–98 Drevet, J.R (2000) Glutathione peroxidases expression ... has been reported [26] Among the GPX family, Table Glutathione peroxidase activities of GPXle1 and GPXha2 towards diﬀerent substrates Glutathione peroxidase assays were performed as described in...

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báo cáo hóa học:" Decreased levels of serum glutathione peroxidase 3 are associated with papillary serous ovarian cancer and disease progression" pot

Ngày tải lên : 20/06/2014, 08:20

... conceptualized and designed the experiments, collected, assembled, analyzed and interpreted data, and drafted the manuscript OC conceptualized and designed the experiments, and collected, assembled and ... designed and implemented experiments SS recruited, collected and annotated specimens, and interpreted data KO recruited, collected and annotated specimens, and interpreted data SC collected and annotated ... specimens, and analyzed and interpreted data PD conceptualized and designed the experiments, analyzed and interpreted data, and helped with the drafting of manuscript JM conceptualized and designed...

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Tài liệu Báo cáo khoa học: A novel dicyclodextrinyl diselenide compound with glutathione peroxidase activity ppt

Ngày tải lên : 19/02/2014, 02:20

... Heterozygous cellular glutathione peroxidase deciency in the mouse: abnormalities in vascular and cardiac function and structure Circulation 27, 11541158 Babizhayev MA (1996) Failure to withstand oxidative ... group on the antioxidant activity of glutathione peroxidase J Am Chem Soc 116, 25572561 13 Mugesh G, Panda A, Singh HB, Punekar NS & Butcher RJ (2001) Glutathione peroxidase- like antioxidant activity ... cytosolic glutathione peroxidase mimic Biochem J 332, 251255 Liu J, Luo G, Gao S, Zhang K, Chen X & Shen J (1999) Generation of a glutathione peroxidase- like mimic using bioimprinting and chemical...

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Báo cáo khoa học: Hydroperoxide reduction by thioredoxin-speciﬁc glutathione peroxidase isoenzymes of Arabidopsis thaliana potx

Ngày tải lên : 16/03/2014, 12:20

... (HaPhGPX), Lycopersicon esculentum (LePhGPX), Chinese cabbage (PHCC-TPx), Setaria italica (SiPhGPX), Raphanus sativus (RsPhGPX), S cerevisciae (yeast GPX2) and non-SeCys type GPX from human and ... catalytic domains (Fig 2) The ﬁrst A thaliana glutathione peroxidases and third Cys residues conserved in yeast GPX2 and Chinese cabbage Trx-dependent peroxidase (TPx) are needed to form a disulﬁde ... and -6 for Trx were 13.4 · 10)2, 11.0 · 10)2, 13.0 · 10)2 and 13.7 · 10)2Æs)1 and Kcat ⁄ Km values were 3.35 · 104, 5.0 · 104, 4.19 · 104 and 5.96 · 104 m)1Æs)1, respectively (Table 2) Vmax and...

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Báo cáo khoa học: cGMP and glutathione-conjugate transport in human erythrocytes The roles of the multidrug resistance-associated proteins, MRP1, MRP4 and MRP5 pot

Ngày tải lên : 23/03/2014, 21:20

... membrane vesicles shown in Fig The maximum uptake rates, Umax1 and Umax2, the dissociation constants, Kd1 and Kd2, and the Hill coefficients, n1 and n2 are defined as indicated in Eqn (1) The data were ... erythrocyte membrane vesicles The maximum uptake rates, Umax1 and Umax2, the dissociation constants, Kd1 and Kd2, and the Hill coefficients, n1 and n2 are as defined in Eqn (1) The data were collected ... Immunodetection of MRP4 and MRP5 in human erythrocytes and COR-L23 cells Inside-out membrane vesicles prepared from human erythrocytes, COR-L23/P and COR-L23/R cells, and crude lysates from MOR/ADR...

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Báo cáo khoa học: "Effects of endocrine disrupting chemicals on expression of phospholipid hydroperoxide glutathione peroxidase mRNA in rat testes" pdf

Ngày tải lên : 07/08/2014, 20:23

... ,M setaY ,D noxiD ,FJ esuoC 8437-2437 ,962 ,4991 mehC loiB J ecneuqes dica onima decuded dna ,ANDc ,AND cimoneG esadixorep enoihtatulg edixorepordyh -dipilohpsohP L éholF ,E rednegniW ,F inisrU ... xPGHP yfilpma ot desu erew stes remirp gniwollof ehT )KU ,secneicsoiB mahsremA( stnegaer sisehtnys ANDc dnarts-tsrif dna sremirp 6)N(dp gnisu debircsnart esrever saw )gµ 5( ANR latoT esu litnu Co07− ... nosbiG ,K eahC ,SK hcaroK 61 506-106 ,01 ,9991 emoneG mmaM gnippam dna ,noisserpxe eussit ,ecneuqes ANDc :esadixorep enoihtatulg edixorepordyh dipilohpsohp eniruM DK neirB'O ,SS beeD ,CR fueoBeL ,M...

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Báo cáo khoa học: "Glutathione peroxidase (GPX) activity in blood of ewes on farms in different scrapie categories in Iceland" pptx

Ngày tải lên : 12/08/2014, 18:22

... selenium and glutathione peroxidase activity in blood of sheep, cows and pigs Res Vet Sci 1976, 20:229-231 Atroshi F, Sankari S, Osterberg S, Sandholm M: Variation of erythrocyte glutathione peroxidase ... Iceland are included with the other results in the present study (cf Materials and methods) Decreased glutathione peroxidase and superoxide dismutase activity in brain of prion-infected mice and ... purposes) Acta Veterinaria Scandinavica 2008, 50:23 http://www.actavetscand.com/content/50/1/23 Figure Scrapie in Iceland and locations of farms Scrapie in Iceland and locations of farms Scrapie...

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Báo cáo khoa học: Puriﬁcation and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx

Ngày tải lên : 23/03/2014, 09:21

... by Edman degradation and MS are in bold letters and boxes, respectively 1796 FEBS Journal 274 (2007) 1793–1803 ª 2007 Merck and Co., Inc Journal compilation ª 2007 FEBS B Ma and F N Chang named ... and healthy controls (open bar) (B) Titration curve of Bla g (open circle) and BgGSTD1 (solid circle) against IgE obtained from patients with cockroach allergy Data represent the mean and standard ... glutathione peroxidase A survey of reported GST-mediated peroxidase activity across insect species revealed that Delta class GSTs probably have higher activity than the enzymes in the Sigma and...

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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx

Ngày tải lên : 21/02/2014, 01:21

... known GSTs classes and S mansoni GSTs, allowed us to place the new parasite product among the newly identified GSTO class, and the previously characterized Sm28GST and Sm26GST as mu- and sigma-class, ... protocols Glutathione and S-hexyl glutathione affinity assays were performed in batch Briefly, 20 lL of 50% resuspended Ó FEBS 2002 5514 J Girardini et al (Eur J Biochem 269) resin was centrifuged and ... was not able to bind to agarose-coupled glutathione, but was able to bind to S-hexyl glutathione agarose and was recovered after elution with S-hexyl glutathione, as was already described for...

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Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx

Ngày tải lên : 07/03/2014, 21:20

... only one Fe-Im band, in contrast to the class I and II peroxidases that have two Fe-Im bands resulting from the tautomerism of the imidazole Nd proton with respect to the donor and acceptor atoms ... rather anomalous hybrid peroxidase, that exhibits the spectroscopic characteristics and substrate preferences of both class I and class III peroxidases [24] As in ascorbate peroxidase (APX) [25], ... changes in Fe-His band frequencies and the redox potential values This is exemplied by the case of CCP and its mutants D235E, D235N and D235A [27] The H-bond between the proximal His and Asp235 is...

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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

Ngày tải lên : 08/03/2014, 16:20

... Ch-Prx1 protein and with BSA (400 lgámL)1) The reactions were stopped by adding 3.3 mM EDTA and analyzed on an agarose gel Thioredoxin-dependent peroxidase activity of Ch-Prx1 Peroxidase activity ... described in yeast, mammals and plants, Ch-Prx1 displayed antioxidant and peroxidase activities The enzyme could reduce hydrogen peroxide as well as alkyl hydrogen peroxide and exerted a strong protective ... mM Na2EDTA, 7% SDS, and 1% BSA After puri®cation of positive clones, k DNA was extracted and puri®ed as described in [13] cDNA inserts were excised by a digestion with EcoRI and cloned into the...

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Báo cáo Y học: The mechanism of nitrogen monoxide (NO)-mediated iron mobilization from cells NO intercepts iron before incorporation into ferritin and indirectly mobilizes iron from ferritin in a glutathione-dependent manner pot

Ngày tải lên : 08/03/2014, 22:20

... hydrazone (PIH) and its analogue, 2-hydroxy-1-naphthylaldehyde isonicotinoyl hydrazone (311), were synthesized by standard techniques [34] Both PIH and 311 are strong Febinding ligands [34] and were ... concentrations of both physiological chelators (apolactoferrin and apoTf) and synthetic chelators (EDTA and DTPA) were used, and for all ligands no signiﬁcant potentiation of 59Fe mobilization was ... (0.01–1 mM) The media and cells were separated and the 59Fe levels in each assessed Cells were lysed and the cytosols subjected to native PAGE-59Fe-autoradiography (see Materials and methods) (B)...

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Báo cáo khoa học: Glutathione transferases kappa 1 and kappa 2 localize in peroxisomes and mitochondria, respectively, and are involved in lipid metabolism and respiration in Caenorhabditis elegans pot

Ngày tải lên : 16/03/2014, 03:20

... kidney, stomach and heart, and its association with liver and kidney mitochondria has been demonstrated by electron microscopy [6] GSTK1 transcript tissue expression is similar in the rat and in the ... expression in human [4] and mouse [6] tissues In order to determine the spatial and temporal expression patterns of gstk-1 and gstk-2 genes in C elegans, we constructed gfp::gstk-1 and gstk-2::gfp fusions ... the body wall muscles (Fig 2C) and in the rectal gland cells in larvae and adults (Fig 2D) The presence of a peroxisomal targeting signal in its C-terminus (Fig 1) and the punctate localization...

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Báo cáo khoa học: Yeast oxidative stress response Inﬂuences of cytosolic thioredoxin peroxidase I and of the mitochondrial functional state pot

Ngày tải lên : 16/03/2014, 14:20

... cytosolic thioredoxin peroxidase II, alkyl hydroperoxide reductase, mitochondrial thioredoxin peroxidase I, cytochrome c peroxidase, glutathione reductase, catalase T and catalase A, did not alter ... isocitrate dehydrogenase, glutathione peroxidase III, heat shock protein 104, thioredoxin peroxidase II, catalase A, nuclear thioredoxin peroxidase, thioredoxin I and glutaredoxin II, respectively ... oligomeric states in vivo cTPxI and cTPxII can act as peroxidases and as molecular chaperones, depending on changes of their quaternary structures triggered by oxidative stress and heat shock exposure...

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Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new afﬁnity label for probing the structure and function of glutathione transferases potx

Ngày tải lên : 16/03/2014, 16:20

... (for rat GST A1-1 and human GST A1-1) or 100 lmol potassium phosphate buffer, pH 7.5 (for glutathione reductase, glutathione synthase and L-lactate dehydrogenase), SDTG (98.2 nmol) and enzyme (typically ... site of the rat glutathione transferases 3–3 and 1–1 and human glutathione transferase A1–1 Biochem J 302, 383–390 Katusz, R.M., Bono, B & Colman, R.F (1991) S-(4-Bromo-2,3dioxobutyl )glutathione: ... native and cysteine 112-modiﬁed glutathione S-transferase A1–1: reassessment of nonsubstrate ligand binding Biochemistry 41, 10920–10927 Simons, P.C & Vander Jagt, D.L (1977) Puriﬁcation of glutathione...

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Báo cáo khóa học: Mechanistic insight into the peroxidase catalyzed nitration of tyrosine derivatives by nitrite and hydrogen peroxide doc

Ngày tải lên : 16/03/2014, 16:20

... controversies: dening both the role of peroxidases in nitrotyrosine formation in vivo using eosinophil peroxidase and myeloperoxidase-decient mice, and the nature of peroxidase- generated reactive nitrogen ... times of and were 11.5 and 12.7 min, respectively, and those of the corresponding nitration products, 3-(4-hydroxy-3-nitrophenyl)-propionic acid and 4-hydroxy-3-nitrobenzonitrile, were 13.9 and 15.7 ... 6.8) in 20 min, from 25 35% in 60 and from 35100% of buffer B in 20 min, and monitored at 280 and 412 nm The lactoperoxidase containing peak was manually collected and the homogeneity of the protein...

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Báo cáo Y học: Role of histidine 42 in ascorbate peroxidase Kinetic analysis of the H42A and H42E variants pptx

Ngày tải lên : 18/03/2014, 01:20

... I for both H42A and H42E slowly returns to a spectrum with a slightly red-shifted Soret band, with wavelength maxima at 405, 514, 550sh and 640 nm for H42A and 406, 516, 552sh and 638 nm for H42E ... mini-plasmid system and sequenced to confirm the desired mutation Automated fluorescent sequencing, using New England Biolabs pUC and malE primers, was performed by the Protein and Nucleic Acid Chemistry ... 1/Ka) and k¢1 is the limiting first-order rate constant at high peroxide concentrations A fit of these data for H42A and H42E to Eqn (7) (Fig 3) yields values for k¢1 and Kd of 4.3 ± 0.2 s)1 and...

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Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx

Ngày tải lên : 23/03/2014, 09:21

... 4, and show PCR reactions run on plasmid DNA harboring CrPrx cDNA, and lanes 1, 3, and show the same using genomic DNA of C roseus Primer pairs were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and ... were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and #GSP-4 (lanes and 4); #GSP-2 and #PFLR-1 (lanes and 6); and #PFLF-1 and #PFLR-1 (lanes and 8) (B) Schematic organization of the CrPrx gene The asterisk ... identity between CrPrx and a number of other class III plant peroxidases (EC 1.11.1.7), notably secretory peroxidases from Avicennia marina (accession number AB049589) and Nicotiana tabacum (accession...

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Báo cáo khoa học: Puriﬁcation and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf

Ngày tải lên : 23/03/2014, 15:20

... 765–770 11 Andersson C, Mosialou E, Weinander R, Herbert H & Morgenstern R (1993) Rat liver microsomal glutathione transferase: studies on structure and function In Structure and Function of Glutathione ... human a and l class GSTs [39] Inhibition by S-hexylglutathione was the same for Peak and Peak isozymes and was stronger than the inhibition of human a GST [39] Hence, both substrate and inhibition ... comparable to largemouth bass (7.0 and 0.5 UÆmg)1 protein for CDNB and cumene hydroperoxide, respectively) [43] and salmonid species (17–28 and 22–37 UÆmg)1 protein for liver and kidney puriﬁed enzymes,...

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Báo cáo Y học: Production and chemiluminescent free radical reactions of glyoxal in lipid peroxidation of linoleic acid by the ligninolytic enzyme, manganese peroxidase pot

Ngày tải lên : 24/03/2014, 04:21

... buffer (pH 0) and the enzyme solution Reactions were started by adding H2O2 and were quantified by monitoring the initial rate of increase in absorbance at 470 nm in the presence and absence of ... by MnP Linoleic acid and aldehydes were reacted with 250 mU of the purified MnP, 0.5 mM of Mn(II) and 50 mM of H2O2 at 20 8C for 1–24 h in 10 mM acetate, formate, lactate and tartrate buffers ... 200 mL) was added and reacted at 35 8C for 0.5 h [17] To this solution, 10 mL of a 10-mM methanol solution of decafluorobenzene (DFB) and a drop of 18-N-sulfuric acid were added and the mixture...

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