... type-3coppers) is responsible ofthe adsorption of light at 330 nm. The T1 c opper domain i s the primary electron acceptor from the reducing substrate and electrons are transferred from this copper to the ... activity (%)pHFig. 8. Effect ofthe pH on the activity ofthe purified laccase. pH in the range of 2.5–8 were tested with 500 lMABTS (d )and1 7lM of syringaldazine ( j) as the su bstrate.Sd 194 ... ammoniumsulfate, and a concentration b y ultrafiltration followed by aMono Q column. All the characteristics ofthe recombinantlaccase are in agreement with those ofthe native laccase. This is the first...
... conclusion is consistent with the verylow degree of identity between the N-terminal sequence of PHI and those of ferredoxin-like proteins belonging to otheroxygenases. On the contrary, the N-terminus ... possibility is that PHI interacts with onePHR and one PHO (abc) protomer. The hypothesis of adirect PHI–phenol interaction is quite unlikely, because of the fact that the addition of phenol does ... operating in the case of PH from A. radioresistens S13,as shown by the lack of alteration in the PHO-ANSfluorescence upon addition of PHI.In summary, while the regulatory components of MMOsact...
... avor the displacement ofthe equilibriumtoward the neutral state of His290.To attest this hypothesis, an Arg213Ala mutant has beenexpressed and characterized. The specific activity of the Ala213 ... alcoholate. The shape of the titration curve ofthe catalytic histidine shows anapparent pKa value ofthe essential histidine estimated to4.1 (Fig. 2C). This acidic pKa shift ofthe histidine ... Indeed, the presence of Arg213 along with the proximity ofthe chargerelay Asp260 within the active site could be responsible for the modification ofthe catalytic properties ofthe catalytichistidine....
... transcription and translation of the reporter gene. Alternatively the cells were left in the presence of the antibody for t he complete length of this period.In both cases the result was the same. As shown ... those of CD53, CD9or CD37 [15]. However, despite the knowledge of some of the eff ects induced by tet raspanin proteins andthe proteinswith which they interact, the identification ofthe s ... the top is a gel from one experiment, showing incorporation of phosphate detectedwith the PY99 mA b andthe total amount of Vav protein det ected byWestern blot. The ratio ofthe PY99 to the Vav...
... resolutions ofthe VIth andthe VIIth congresses ofthe Communists Party and those ofthe important meeting ofthe National Assembly, the Government andthe CentralParty Committee emphasized the importance ... turnimprove the lives of individuals, their families and society as a whole. The attainment of this objective requires the improvement ofthe educational and professional level of human resources, ... planning and management with expertise in both theoretical and practical aspects of their field is a keyissue for accelerating the renovation ofthe education system in Vietnam. This core group of specialists...
... understand the strategic position ofthe organization. There is strategic choice which is to do with the formulation of possible courses of action, their evaluation andthe choice between them.Finally ... mechanical85 Chapter 4Internal Analysis The final goal of this chapter is that, through the deep analysis of Hanoi MechanicalCompany to reveal the strengths and weaknesses ofthe company in order to find ... non-synchronous and understandardized products, high production costs and faced the rejection ofthe market. All of these attributes are differentfrom conditions for the development of Machinery...
... beproposed on the basis ofthe results presented here.Two ofthe mutants (D217E and E221Q) showed noactivity in any ofthe assays. These mutants (and wild-type) were assessed for their ability ... each of these in order to dissect their contributions to the isomerase and reductase activities. Several mutations result in loss of the isomerase activity with retention of reductase activity. However, ... aeruginosa KARI [11]. The tertiary and quaternary organization of this enzyme is substantially different from that ofthe spinach enzyme with the active site constructed from two monomers of a dode-camer....
... coffee at the price it is now? I guess she doesn’t,” said her father. He poured the coffee-pot full of boiling water from the tea-kettle, then he tipped the coffee canister into his hand, and ... that isthe coffee canister,” said she, pointing. “Why didn’t you say so, then?” asked her father, viciously, and again Maria made no reply. Her father seized the coffee canister and By the ... softly, and crept across the hall to the spare room, which Aunt Maria had occupied ever since she had been there. She listened, and heard a soft sob. Then she turned the knob of the door softly....
... the intracellular segment ofthe b1subunit is required for the interaction with the a subunit, probably acrucial step for the regulation of channel properties. The tissue distribution ofthe ... where Gmax is the maximumconductance, z isthe valence ofthe process and Vẵ is the midpoint voltage of activation.Ionic current expression levels were determined from batches of oocytes injected ... the N-terminal sequence ofthe human b1. However, the C-terminal sequence was significantly differentfrom that of the human b1, suggesting that it might encode a novelsplice variant of the...
... Characterization ofthe TRH-DE from serum and liver (Eur. J. Biochem. 269) 1281 Western blot analysis. To verify the hypothesis that the brain T RH-DE, the serum TRH-DE andthe liver TRH-DEarederivedfromthesamegene,theenzymepreparationswere ... is very similar oridentical. These results s trongly support the hypothesis that the serum TRH-DE andthe membrane-bound TRH-DE from brain and liver are derived fromthe same gene,whereby the ... between the particulate liver enzyme and the serum enzyme were noticed [9,15].To verify the hypothesis that the serum TRH-DE is of liver origin we analyzed the TRH-DE i n serum and tissue ho...
... FSW and improve knowledge and pratising STD managing for health workers, base on that to expand this intervention model for other centers over the nation. 4. The layout ofthe thesis The thesis ... characteristics of FSW is the nature mobility, due to the inferiority of their career, they do not want to live in a place fixed for a long time. On the other hand they also have to move from ... LRTIs in FSWs. The results of some other domestic and foreign studies were comformed with the results of this study to point out the high rate of LRTIs. Reported by WHO, the incidence of sexually...