hot spots in the gyrb binding cavity

Báo cáo y học: "Conformational alterations in the CD4 binding cavity of HIV-1 gp120 influencing gp120-CD4 interactions and fusogenicity of HIV-1 envelopes derived from brain and other tissues" pptx

Ngày tải lên : 13/08/2014, 01:20

... which becomes part of the gp120 bridging sheet, which is a structural element of gp120 formed after CD4 binding that is involved in coreceptor binding [3,11] Changes in CD4 binding to gp120 contribute ... residues within three separate regions distributed over six segments of gp120 These regions include the a-helices of the inner domain, the CD4 binding loop of outer domain, and the b20-b21 ribbon ... measuring the distance between the inner most atoms present at the stem of the V1V2 loops and the V5 loop, which constrain the CD4 binding pocket of gp120 [9] Figure shows the derivation of the CD4bs...

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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

Ngày tải lên : 19/02/2014, 13:20

... concentration The dissociation constants, Kd values, enthalpies of binding and stoichiometries were determined from the binding isotherm by ﬁtting a : binding model to the data using the software ... uncharged valine side chain completely abolishes fosfomycin binding in the absence of UDPNAG Clearly, UDPNAG binding creates additional favorable interactions that promote fosfomycin binding to the enzyme ... mutant proteins This was interpreted as a lack of fosfomycin binding to these mutant proteins [12] Binding of fosfomycin to either wild-type MurA or the K22 mutant proteins in the absence of UDPNAG...

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707
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Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulﬁde isomerase by disulﬁde bond formation with free cysteines in the substrate-binding domain pptx

Ngày tải lên : 14/03/2014, 23:20

... [16] tested the effect of bacitracin on the reductive activity of a truncated PDI containing the catalytic a-domain, but lacking the independent substrate -binding b¢-domain Bacitracin did not ... cysteines In conclusion, we show a mechanism of action of bacitracin on PDI that involves covalent binding of an open thiol form of bacitracin to free cysteines in the substrate -binding domain ... bacitracin in this region would impair the binding of the substrate insulin, ultimately inhibiting its reduction Although all bacitracin analogs seem to interact covalently with PDI (Fig 4), there...

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626
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Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf

Ngày tải lên : 15/03/2014, 09:20

... C-terminal domain, including the highly conserved GSH -binding site (G-site) and the more divergent cosubstrate -binding domain or hydrophobic binding site (H-site) [7,18] The G-site includes both a-helices ... secondary structure elements The topological arrangement of these elements is usually bababba, similar to the thioredoxin fold of other GSH -binding or cysteine -binding proteins The H-site is entirely ... sensitive to the nature of the leaving group, as the substitution of the chlorine atom with the more electronegative ﬂuorine in the CDNB molecule did not affect the kcat values Altogether, these results...

8
421
1

Báo cáo khoa học: Intermonomer cross-linking of F-actin alters the dynamics of its interaction with H-meromyosin in the weak-binding state ppt

Ngày tải lên : 16/03/2014, 13:20

... complexes the motion of the probe is less restricted relative to the strong binding state and there is no difference between the two weak binding complexes In the case of cross-linked F-actin, the ... subdomain 1, where the spin probe is attached, is not directly involved in the intermonomer cross-link, because one of the cross-linking sites is occupied by the spin probe, therefore, the rigidity ... cross-linked samples In the control F-actin samples, there are differences in the motion of the EPR probe when the acto-HMM complex is in a strong and weak binding state, respectively Under strong binding...

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311
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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

Ngày tải lên : 23/03/2014, 03:20

... at the junction of the two barrels Another conserved characteristic of lobster trypsins is the presence of calcium -binding sites (Fig 3C) The calcium -binding motif does not occur in many invertebrate ... despite the presence of calcium -binding sites, Ca2+ affected neither the activity nor the stability of crab trypsin because there are no accessible autolysis sites in the N-terminal domain, which ... different in comparison with those of vertebrate trypsins, and also they are important for inhibitor binding [8] The phenylalanine and Ile residues in crayﬁsh Loop37 interact with the C-terminal segment...

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474
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Báo cáo khoa học: Crystal structures of HIV protease V82A and L90M mutants reveal changes in the indinavir-binding site potx

Ngày tải lên : 23/03/2014, 12:20

... propagating to the inhibitorbinding site Fig Structural variation in residues 81–82 near indinavir Stereoview showing the benzyl group of indinavir interacting with residues 81–82, using the major ... the values in the two mutants Ile50¢ was ordered in all three structures and the side chain ﬁtted well in the S2 binding pocket created by the t-butyl group of indinavir Ile50, on the other hand, ... shown in A (A) Comparison of indinavir interactions with Arg8¢ in the wild-type HIV-1 protease (PR) (black) and PRL90M (gray) (B) PR interactions with indinavir The indinavir bonds are in black, the...

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355
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Different roles of functional residues in the hydrophobic binding site of two sweet orange tau pps

Ngày tải lên : 07/08/2014, 01:20

... C-terminal domain, including the highly conserved GSH -binding site (G-site) and the more divergent cosubstrate -binding domain or hydrophobic binding site (H-site) [7,18] The G-site includes both a-helices ... secondary structure elements The topological arrangement of these elements is usually bababba, similar to the thioredoxin fold of other GSH -binding or cysteine -binding proteins The H-site is entirely ... sensitive to the nature of the leaving group, as the substitution of the chlorine atom with the more electronegative ﬂuorine in the CDNB molecule did not affect the kcat values Altogether, these results...

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385
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Báo cáo y học: " Second site escape of a T20-dependent HIV-1 variant by a single amino acid change in the CD4 binding region of the envelope glycoprotein" doc

Ngày tải lên : 13/08/2014, 09:20

... domain Binding of the surface subunit gp120 to CD4 and a co-receptor on the T-cell surface triggers conformational changes in the Env complex, leading to the insertion of the hydrophobic N-terminal ... 3:84 interaction with the HR2 region in gp41 Alam et al [16] found an HR2/T20 peptide -binding site on soluble HIV-1 recombinant gp120 Furthermore, they demonstrated that binding of T20 was induced ... to mimic the C4 region in gp120 have the ability to suppress the T20 inhibitory effect either by preventing T20 from binding to the C4 region in gp120 and/or by modulating gp41 conformational...

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364
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Báo cáo y học: "Molecular defects in the mannose binding lectin pathway in dermatological disease: Case report and literature review" pps

Ngày tải lên : 13/08/2014, 13:22

... bacteria on binding of the pattern recognition molecules mannan -binding lectin, L-ficolin, and Hficolin Infect Immun 2005, 73:1052-1060 Written Consent Written informed consent was obtained from the ... activation and opsonophagocytosis by complexes of mannose -binding lectin with mannose -binding lectin-associated serine protease after binding to Staphylococcus aureus J Immunol 2002, 169:4430-4436 ... mannose -binding protein Structure 1994, 2:1227-1240 Madsen HO, Satz ML, Hogh B, Svejgaard A, Garred P: Different molecular events result in low protein levels of mannan -binding lectin in populations...

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346
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Báo cáo y học: "Hot times in the intensive care unit" pdf

Ngày tải lên : 13/08/2014, 21:20

... harmful, there is little evidence that the more modest fevers commonly seen in ICU patients aﬀect their clinical outcomes Until we have such data, there seems to be little use in treating fever ... as the development of fever may provide an important clue alerting us to the need for modifying therapy for pathophysiologic processes, such as a new infection, that really can determine the ... prolonged period and in more typical ICU populations before its routine use can be considered The question of whether we should be treating fever at all remains Although there may be some reason...

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Epistemic modality manifested in conversation in the thorn birds = tính chân nguỵ biện được thể hiện qua lời thoại trong tác phẩm tiếng chim hót trong bụi mận gai

Ngày tải lên : 20/12/2013, 18:24

... shows the tendency of the characters employing modal verbs to commit themselves or the others to the actions equal to the way they use them to express feeling, attitudes, as well as cling to the ... 75,47% sure is the one having the highest occurrence to state something certain or not according to the subjective idea of the S and it mostly occurs in the main clauses of the subordinate clauses ... decide to choose the topic Epistemic modality manifested in conversations in The Thorn Bird as the theme for our thesis Aims of the study The thesis has been done with the following aims: - To...

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Tài liệu Báo cáo khoa học: The role in the substrate speciﬁcity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx

Ngày tải lên : 18/02/2014, 17:20

... was further investigated by determining the in uence on the glycone binding of the Sfbgly interactions with the aglycone Thus, the effect of the alteration of the glycone structure in the stability ... in an internal region of the aglycone -binding site, probably subsite +1, because they in uence the binding of the ﬁrst glucose unit of the aglycone On the other hand, M453 may be positioned in ... of the aglycone -binding site in the stabilization of ESà and the interdependence between the binding of aglycone and the positioning of glycone in subsite )1 are not known In this study, the...

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731
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

Ngày tải lên : 19/02/2014, 06:20

... histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although there are four histidines in the C-terminal, another ... (Fig 2) The binding isotherm data were fitted to sequential binding site parameters and the best fit, producing the smallest v2 values, indicated two binding sites, both with affinities in the picomolar ... copper binding on amyloid formation of other proteins A number of other amyloidogenic proteins have been shown to be copper binding proteins Copper binding is sometimes specific and at other times...

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586
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Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-afﬁnity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt

Ngày tải lên : 19/02/2014, 12:20

... of the ephrinB2(301–333) peptide alter only the binding interactions and the three-dimensional structure of the Grb4 -binding region, i.e residues 301–322, while leaving the PDZ -binding C-terminus ... with the Grb4 SH2 domain, i.e the binding isotherms (Fig 4A) The ephrinB2(301–333)pY304 peptide was found to have the strongest binding to the Grb4 SH2 domain while the other five peptides, ephrinB2(301–333), ... detect the binding interactions between the peptides and the Grb4 SH2 domain The affinity of the binding interactions was evaluated by measuring the changes of fluorescence polarization of the peptides...

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551
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$Tài liệu Báo cáo khoa học: Identiﬁcation of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line speciﬁcally binding to aptameric cytotoxic GT oligomers ppt$

Tài liệu Báo cáo khoa học: Identiﬁcation of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line speciﬁcally binding to aptameric cytotoxic GT oligomers ppt

Ngày tải lên : 21/02/2014, 00:20

... protein belonging to the GTPbinding elongation factor family, which promotes the GTPdependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis and the capture of ... quantify the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the buffer Therefore, a ﬁxed aliquot of the protein was incubated with the indicated ... cancer cells One hypothesis may be that these oligomers exert their action by blocking the binding of eEF1A to its ligand in cancer cells, perhaps to zinc ﬁnger proteins involved in the modulation...

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552
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Tài liệu Báo cáo Y học: The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region ppt

Ngày tải lên : 22/02/2014, 04:20

... cycle-dependent binding of LBR to chromatin Fig Identiﬁcation of chromatin binding domains in the N-terminal region of LBR and analysis of the binding mode Empty columns: four kinds of GST fusion proteins including ... chromatin in eggs, binding through the RS region of LBR to linker DNA of chromatin seems to be predominant Then, the binding is supported by the interaction of the NM region and a protein(s) other ... characterize the mode of binding of LBR to chromatin, beads bearing LBR fragments were preincubated with a DNA solution and then the binding to chromatin was examined (Fig 3, hatched columns) The binding...

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563
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Báo cáo khoa học: Starch-binding domains in the CBM45 family – low-afﬁnity domains from glucan, water dikinase and a-amylase involved in plastidial starch metabolism pptx

Ngày tải lên : 06/03/2014, 00:21

... b-cyclodextrin injection, and the bottom panel depicts the binding isotherm; open circles represent the integrated binding heat of the data in the top panel, and the full line is the fit of a one-site binding ... the starch -binding ability of AtAMY3 in vitro It cannot be precluded that some binding may involve secondary binding sites in the catalytic domain, but the demonstrated starchbinding ability ... showed no binding to maltoheptaose Taken together, the data presented here show that the binding affinity of the 1180 The full-length AtAMY3 offered an advantage when examining the binding affinity...

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634
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

Ngày tải lên : 07/03/2014, 01:20

... cell death [65,66] The AR contains an N-terminal domain harboring activation function 1, a central DNA -binding domain (DBD) and a C-terminal ligandbinding domain (LBD) containing activation function ... ligand -binding domain Actin -binding protein Targeting sequence Gelsolin Direct or indirect association with the AR Region LBD Classes Role in the cytoplasm AR effect Mechanism ()) Actin ﬁlament ... Direct a-actinin-2 ()) Bundling proteins Coactivator Supervillin NLS F-actin- and membraneassociated scaffolding protein Filamin NLS? Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin...

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Báo cáo khoa học: Inactivating pentapeptide insertions in the ﬁssion yeast replication factor C subunit Rfc2 cluster near the ATP-binding site and arginine ﬁnger motif docx

Ngày tải lên : 07/03/2014, 02:20

... an arginine residue located in Rfc4 (RFC-B) The side chain of the arginine is referred to as an arginine finger and the finger protrudes into the ATP -binding site of the neighbouring subunit The ... whereas insertion F45 disrupts a8 These insertions are likely to affect the positioning of the arginine finger in site D, thereby blocking DNA binding by RFC–PCNA Of the remaining three inactivating ... protein Mapping the insertions onto the structure of Rfc2: insertions into the N-terminal AAA+ domain The 3D structure of the Rfc2 protein comprises three separate domains Domains I and II together...

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