host vector combinations for production of membrane proteins
05 bacterial cultivation for production of proteins and other biological products (1)
Ngày tải lên :
24/04/2016, 10:44
... 10 Bacterial Cultivation for Protein Production N 133 FIGURE General layout of the bacterial cultivation process for production of proteins and other biological products 10.2.2 ... phosphorus for the formation of RNA and DNA, and sulfur for the formation of the amino acids methionine and cysteine Other trace elements, such as metal ions, are required by various enzymes for their ... equipment, and (v) performing the production process itself A large amount of work has to be done on development and optimization of the production process before commercial production can be initiated...
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Báo cáo khoa học: "Project for production of closed-caption TV programs for the hearing impaired" docx
Ngày tải lên :
08/03/2014, 06:20
... selected for the evaluation of the model We used the speech of four people for the evaluation The evaluation results are shown in Table They are the accuracy (detection rate) and false alarm rate for ... their news program for the purpose of our research References Figure Captions in the picture Figure Captions outside of the picture Most of the subjects preferred 2-line, outside of the picture captions ... Department of Information and Computer Science (master's thesis) in Japanese Wakao, T., Ehara, E., Sawamura, E., Abe, Y., Shirai, K (1997) Application of NLP technology to production of closed-caption...
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APPLYING OF ULTRASOUND FOR PRODUCTION OF NANOMATERIALS
Ngày tải lên :
11/06/2014, 12:29
... on the base of ultrasonic system with power kW of LLC «Ultrasonic technique - INLAB» production (fig 1) have developed sonochemistry reactor (USA patent №7,157,058 B2) for production of nano-particles ... sonochemically, for example, nano-particles of Fe, Co, Cu, Ag, Ni, Pd and so on The reactor is an effective unit for accelerating of chemical reactions, for example, the reduction of metal salts ... XXII Session of the Russian Acoustical Society Session of the Scientific Council of Russian Academy of Science on Acoustics Moscow, June 15-17, 2010 are added to the mixture to form the amorphous...
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Improved oxides for production of leadracid battery plates pot
Ngày tải lên :
05/07/2014, 21:20
... temperature for oxidation to the required product In the case of pure b-PbO, the temperature is held at 6008C while for the production of red lead a range of 450 to 5008C is used In the case of red ... material feedstock for the production of pure PbO and red lead A schematic flow chart of the process used to Red lead is beneficial in improving the electrochemical performance of cells and batteries ... 0.1 26–32 125–150 4.5 20 90 Fig Schematic diagram of flow process for production of leady oxide, lead monoxide, and red lead D.P Boden r Journal of Power Sources 73 (1998) 56–59 produce leady oxide,...
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Báo cáo vật lý: "Protonation and Complexation Approaches for Production of Protic Eutectic Ionic Liquids" ppsx
Ngày tải lên :
07/08/2014, 14:20
... 251.46 50.17 The DSC data for the PEILs were taken for appropriate molar ratios of the complexing agent (two for urea and one for oxalic acid) owing to the appearances of the PEILs that, overall, ... than the information concerning the hydrochloride salts that are related to the preparation Journal of Physical Science, Vol 21(1), 15–28, 2010 17 of ILs For that reason, the concept of hybrids ... medications Combinations of hydrochloride salt and complexing agents to form ILs have never been reported before In this study, we produced protic eutectic ionic liquids (PEILs), a subgroup of the...
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Báo cáo vật lý: "Preliminary Studies for Production of Fatty Acids from Hydrolysis of Cooking Palm Oil Using C. rugosa Lipase" pot
Ngày tải lên :
07/08/2014, 14:20
... Hydrolysis Profile Figure shows a typical hydrolysis profile of palm oil at temperature of 45ºC for an initial palm oil concentration of 0.1 g palm oil/ml iso-octane at agitation speed of 200 rpm ... every 30 from each flask 2.3 Determination of Degree of Hydrolysis (Conversion) and Rate of Hydrolysis The degree of hydrolysis was determined by titration of the oil phase samples with 0.1 M sodium ... indicator The degree of hydrolysis, X is calculated as below: X,% = (ml NaOH used) (molarity of NaOH) (average molecular weight of fatty acid) 10 (weight of sample) (1) The rate of hydrolysis, r...
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Báo cáo khoa học: "Two-dimensional gel electrophoresis of membrane proteins from ectomycorrhizal fungi" doc
Ngày tải lên :
08/08/2014, 23:22
... investigation develop suitable methods for the isolation of a membrane fraction free of mitochondrial or nuclear membranes and for efficient solubilisation of membrane proteins in order to analyze them ... bulk membrane fraction is required for detailed studies of specific membrane changes during mycorrhizal formation However, sampling of thousands of ectomycorrhizas needed for a purification of ... Solubilisation of membrane proteins for 2-D PAGE is difficult because most membrane proteins are tightly bound to membrane lipids, apparently by hydrophobic and ionic bonds The choice and the quantity of...
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Structural genomics of membrane proteins pptx
Ngày tải lên :
09/08/2014, 20:20
... target genomes for membrane- protein structural genomics efforts [13] The initial goals of these efforts will be to clone, overexpress and purify the known and putative membrane proteins of their selected ... some strain of E coli will serve as host cell Although there are a number of strains that have been used to express membrane proteins, BL21 (DE3) and a derivative of BL21 optimized for membrane- protein ... this class of vectors will be favored by research groups overexpressing membrane proteins One concern, especially with respect to the overexpression of membrane proteins, is the effect of target-protein...
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báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps
Ngày tải lên :
11/08/2014, 00:23
... considerable amount of non-specific proteins eluted in conjunction with this protein Final purity of the purified proteins is without any doubt an excellent measure of the performance of protein purification ... SiMAG/CS-NTA/Nickel for purification of two Histagged recombinant proteins, His-ProT and His-Mre11, overexpressed in Escherichia coli Results Relative expression of His-tagged recombinant proteins Recombinant proteins ... concentration of the bead As with His-tagged proteins, total content of non-specific proteins in FT decreased with increasing the concentration of SiMAC-Nickel beads indicating non-specific binding of non-target...
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Báo cáo y học: " Increased production of viral proteins by a 3''''-LTR-deleted infectious clone of human T-cell leukemia virus type 1" ppsx
Ngày tải lên :
12/08/2014, 04:21
... expression patterns (A) Diagram of the molecular clones showing the deletion series of the 3' LTR and the deletion of the reverse TATA box of the 3' LTR (B) The amount of p19 gag in the pooled concentrated ... Ishioka et al reported that inactivation of DLG1 augments the Tax-mediated transformation of cells This finding suggests that DLG1 regulates the function of Tax through its PDZ-binding motif [26] ... cellular factors, such as PDZ proteins, inhibiting Tax expression by binding to the PDZ-protein-binding motif in the C-terminal portion of the tax gene Page of (page number not for citation purposes)...
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Báo cáo y học: "The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins" pptx
Ngày tải lên :
14/08/2014, 17:22
... 488 proteins to be annotated as membrane proteins (47% of all proteins with localization information) Of these proteins, 225 proteins were annotated as plasma membrane proteins (21.6%) These proteins ... Origin of proteins in the urine Our analysis revealed that extracellular proteins, plasma membrane proteins, and lysosomal proteins are enriched in The excretion pathway of renal apical plasma membrane ... plasma membrane proteins and lysosome proteins interactions Figure urine from nine persons Comparison of identified proteins in urine of a single person and pooled Comparison of identified proteins...
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production of recombinant proteins by microbes and higher organisms demain 2009
Ngày tải lên :
08/09/2015, 22:11
... ease of culture and high product yields (Swartz, 1996) It is used for massive production of many commercialized proteins This system is excellent for functional expression of nonglycosylated proteins ... strains of B subtilis are high secretors and host strains used for successful expression of recombinant proteins are often deleted for genes amyE, aprE, nprE, spoIIAC, srfC and transformed via ... 20–30 g/l of recombinant proteins (Morrow, 2007) There are however, some disadvantages of using Pichia as a host for heterologous expression A number of proteins require chaperonins for proper...
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Yeast systems biotechnology for production of value added biochemicals
Ngày tải lên :
09/09/2015, 10:15
... p0 Frequency of occurrence of codon k in the host p1k Frequency of occurrence of codon k in the target coding sequence p0 Vector of frequencies defining codon distribution of the host s selected ... genes p1 Vector of frequencies defining codon distribution of the target coding sequence k q0 Frequency of occurrence of codon pair k in the host s selected genes q1k Frequency of occurrence of codon ... target coding sequence q0 Vector of frequencies defining codon pair distribution of the host s selected genes xiii q1 Vector of frequencies defining codon pair distribution of the target coding sequence...
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Production of recombinant proteins by microbes and higher organisms demain 2009
Ngày tải lên :
09/09/2015, 12:14
... ease of culture and high product yields (Swartz, 1996) It is used for massive production of many commercialized proteins This system is excellent for functional expression of non-glycosylated proteins ... strains of B subtilis are high secretors and host strains used for successful expression of recombinant proteins are often deleted for genes amyE, aprE, nprE, spoIIAC, srfC and transformed via ... 20–30 g/l of recombinant proteins (Morrow, 2007) There are however, some disadvantages of using Pichia as a host for heterologous expression A number of proteins require chaperonins for proper...
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Production of recombinant proteins by microbes and higher organisms
Ngày tải lên :
09/09/2015, 12:34
... ease of culture and high product yields (Swartz, 1996) It is used for massive production of many commercialized proteins This system is excellent for functional expression of non-glycosylated proteins ... strains of B subtilis are high secretors and host strains used for successful expression of recombinant proteins are often deleted for genes amyE, aprE, nprE, spoIIAC, srfC and transformed via ... 20-30 g/l of recombinant proteins (Morrow, 2007) There are however, some disadvantages of using Pichia as a host for heterologous expression A number of proteins require chaperonins for proper...
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Structural basis for recruitment of traffic proteins by small GTPases
Ngày tải lên :
16/09/2015, 08:31
... pinching-off to form a vesicle With the help of these proteins around the neck, the two noncytosolic leaflets of the membrane are brought close to each other, fuse and then pinch off to form the ... formation of the donor membrane requires the assembly of specific proteins coating the cytoplasmic face of the donor membrane, which provides the mechanical force to pull the membrane into a bud ... STRUCTURAL BASIS FOR RECRUITMENT OF TRAFFIC PROTEINS BY SMALL GTPASES WU MOUSHENG (B.Sc., M.Sc) Xiamen University A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL...
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Heterogeneous catalysts for production of chemicals using carbon dioxide as raw material
Ngày tải lên :
08/10/2015, 23:14
... case of Cu and Au, the addition of large amounts of CuO and AuO led to the formation of large pore diameter of catalysts in contrast with Ag loading [26] This could be attributed to the formation ... remarkable performance three times greater than that of Cu/SiO2 due to the role of ZnO/SiO2 in creating Cu þ and Cu0 as active species in driving the hydrogenation steps for the production of methanol ... in the synthesis of methanol from CO2 [12,13] The preparation of Cu/ZnO catalyst by physical mixture of Cu/SiO2 and ZnO/SiO2 resulted in formation of the ZnOx on the surface of Cu particles to...
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Methor statement for production of super - T Girder
Ngày tải lên :
08/05/2016, 10:10
... hydraulic jack for removal inner form Using hydraulic jack for movable of inner form to separate inner form and girder, then using gantry crane to lift core of inner form out of girder In Page ... Statement for Production of Super-T Girder Water pump 2HP set 02 For curing concrete set 03 Production Casting bed type 38.3m girder Outer formwork type 38.3m set 03 Production Inner formwork ... Drawing of frame system for hanging inner formwork 8) Appendix – 8: Drawing of detail for lifting hook 9) Appendix – 9: Drawing of protection frame during stressing 10) Appendix – 10: Calculation of...
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Procedures guidelines procedures for production of UHT chocolate milk beverage
Ngày tải lên :
08/09/2016, 09:52
... Guidelines Procedures for the Production of UHT Chocolate Milk Beverages Introduction With the exception of milk powders several other dry ingredients are used in the production of chocolate milk ... number of decimal reductions of thermoresistant spores Therefore an acceptable level of defects should be established to rate the quality of the final product The maximum acceptable defect rate for ... Guidelines Procedures for the Production of UHT Chocolate Milk Beverages 3.4 Flowchart A typical flowchart for chocolate milk production Weigh the ingredients according to the formulation If the...
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Evaluation of cDNA libraries from different developmental stages of schistosoma mansoni for production of expressed sequence tags (ESTs)
Ngày tải lên :
11/11/2016, 15:59
... use this Rates of 50% in acquirement of new genes as observed library for large-scale EST production The high profor the S mansoni libraries met the criteria established portion of redundant genes ... code for enzymes of different metabolic pathways, a great variety of ribosomal proteins, several constituents of transcriptional/translational machinery, and regulatory cytoplasmic and membrane proteins, ... isolated, even after the discovery of a measure of the proportion of useless clones In general, significant degree of redundancy (65% of the sequenced the libraries were of good quality with respect...
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