... gene containing a trinucleotide repeat
that is expanded and unstable on Huntington’s disease
chromosomes. Cell 72, 971–983.
6 Young AB (2003) Huntingtin in health and disease.
J Clin Invest 111, ... neurodegenerative
diseases caused by CAG repeat expansions, encoding
polyglutamine tracts in different proteins, suggesting
that these diseases may involve overlapping molecular
mechanisms of pathogenesis involving ... progress has been made
in identifying genetic factors contributing to PD in
recent years [135]. Low concordance for clinical disease
in monozygotic twins indicates environmental in uen-
ces on PD...
... been
reported using the proteomic approach, including a
chitinase and a PR protein in Coffea canephora and a
quinone reductase 2 in Gossipium hirsutum [74].
Understanding plant pathogen
interactionsin the ... commonly
identified in several plantpathogen proteomic studies
described in this review (Fig. 1).
Fig. 1. Overview of plantpathogeninteractions and insights into proteomic studies of the proteins involved in ... Thaumatins are pro-
teins involved in the plant response against fungal
infection, and may equally be expressed in peach as a
response to PPV infection [6]. MDLs are flavoproteins
involved in the...
... hypothetical ORFs inplant pathogen
Erwinia carotovora subsp. atroseptica SCRI1043
Ling-Ling Chen, Bin-Guang Ma and Na Gao
Shandong Provincial Research Center for Bioinformatic Engineering and Technique, ... 7e-118 287 S 87 YicC N-terminal domain protein
ECA0264 1e-166 333 R 83 Twin-arginine translocation pathway signal
ECA0285 3e-153 285 R 96 Predicted P-loop-containing kinase
ECA0293 2e-151 357 MR ... basis of our method for distinguishing
between protein-coding and noncoding ORFs.
The difference between coding and noncoding
sequences can be viewed intuitively using PCA. PCA
defines the correlation...
... Farming of Antibodies in Plants 39
Minibody
Fab fragment Single chain Fv
fragment (scFv)
Diabody Bispecific scFv
Camelid heavy chain
scFv-fusion
Single
variable
domain
Large single chain
Antigen ... glycine betaine was
a critical element in the efficient enhancement of tolerance to salt and cold stresses
in the engineered rice plants.
Metabolic engineering to accumulate osmoprotectants in plants ... Overcoming Rate-Limiting Steps
The most important aspects in metabolic engineering are to identify enzymes that
may be involved in intermediate biosynthesis and subsequently to determine if
any...
... in all
cases of Alzheimer disease. These findings not only further define the cell cycle reentry phenotype in Alzheimer
disease but also indicate that the neurofibrillary tangles which define ...
working with other factors to halt the cell cycle and
mediate DNA repair. Interestingly, a much higher
proportion of NFT were labeled in AD cases than in
control cases. These findings hint ... fixed either in 10% buffered formalin or in
methacarn (methanol:chloroform:acetic acid, 6:3:1),
and embedded in paraffin. 6μm sections were depar-
affinized in xylene and rehydrated in graded...
... adducts. In formation of
these addition compounds, important roles are played by hydrogen-bonding
and hydrophobic interactions. In certain cases one of the reacting partners
will wind up in a relatively ... 2000
,
722
Chapter 10
Host- guest chemistry in aqueous organometallic
catalysis
10.1 Cyclodextrins and the formation of inclusion
compounds
Host- guest complexation relies on interactions of molecules ... form an interesting class of macrocycles possessing a
cone-shaped cavity defined by four symmetrically situated phenoxy rings.
Much attention has been devoted to the use of such molecules in host- guest
chemistry...
... blocking or presenting of
binding sites for the ribosome [4]. These are just a few
Keywords
mode of binding; proteins that promote
RNA folding; RNA chaperones; RNA folding
problem; transient interactions
Correspondence
B. ... changes in the RNA are
introduced during the transient interaction arises. Ini-
tial results indicate that the protein acts as an electro-
static lubricant that shields repulsive interactions
within ... helicases. The binding sites for double-
stranded RNA and ATP overlap, resulting in coupled
binding of both molecules. Simultaneous binding
forces the RNA into a bent conformation that is
incompatible...
... structure of the cobalamin-binding domain
Homocysteine
Methionine
Co
Methylcob(III)alamin
Cob(I)alamin
Primary
turnover
cycle
H
CH
3
H
4
folate
AdoHyc
Cob(I)alamin
Cob(II)alamin
H
4
folate
Co
NADPH
A
doMet
e
–
FAD
FMN
NADPH
NADP
+
e
–
Reactivation
Co
Fig. ... 53–56.
34 Tollin G (1995) Use of flavin photochemistry to probe
intraprotein and interprotein electron transfer mecha-
nisms. J Bioenerg Biomembr 27, 303–309.
Supporting information
The following supplementary ... CH
3
-H
4
-folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark grey circle).
hMS is inactivated approximately every 200–1000 catalytic turnovers [owing to...
... strong, neutralizing action of the cytosolic RNase
inhibitor (cRI), a 50-kDa protein containing 16 leu-
cine-rich repeat motifs [7,8]. In fact, onconase from
Rana pipiens eggs [9], and seminal RNase ... proteins, with high
isoelectric point values. Thus we investigated the effects
Table 1. Natural and engineered RNases investigated in this study. IC
50
is the RNase concentration producing half-maximal ... homologous to the RNases investigated in
this study, have been isolated from human eosinophils,
namely eosinophil-derived neurotoxin (EDN) and eosi-
nophil cationic protein (ECP). They are RNases...
... in
point, formation of huntingtin-containing inclusion
bodies in Huntington’s disease encourages cell survival,
whereas monomers and small inclusion bodies of hunt-
ingtin, a protein possessing ... cells deficient in
desmin, again demonstrating inclusion body construc-
tion independent of intermediate fialments [62]. Inter-
estingly, inclusion body formation is slowed by
aB-crystallin, Hsp27 and ... aB-Crystallin R120 corresponds to aA-crystal-
lin R116 and both are conserved a-crystallin domain
arginines. R120G aB-crystallin permits intermediate fil-
ament self association in vitro, although binding...
... Gerstoft J, Pedersen C. Pharmacokinetic
interaction between rifampin and the combination of indinavir and low-dose ritonavir in HIV-
infected patients.
Clin Infect Dis.
Feb 1 2004;38(3):426-429.
26. ... together
Delavirdine AUC
by 95%
Etravirine
Etravirine and rifampin should not be used together
Marked decrease in etravirine predicted, based on data on the
interaction with rifabutin
Single protease inhibitors
Recommended ... Increasing nevirapine dose can
overcome reduced bioavailability due to rifampicin coadministration.
J Acquir Immune Defi c Syndr.
May 2006;42(1):36-41.
6
Predicting Drug Interactions Involving...
... recognition in the normal splicing
process [55]. First, U1snRNP binding to an intronic
splicing processing element has been found to inhibit
pathological pseudoexon inclusion in intron 20 of ... splice-
supporting intronic mutation in the last bp position of
a cryptic exon within intron 6 of the CYBB gene
induces its incorporation into the mRNA causing
chronic granulomatous disease (CGD). ... degradation following its joining to exon 3 (but not exon 2). The levels
of hnRNP H ⁄ F proteins can regulate the extent of NE inclusion. (B) shows that in the NF-1 intron, 30 pseudoexon inclusion levels...
... may be important for plant cells. On the other hand,
the N-terminal BRCT domain proposed to mediate
protein–protein interactions involved in DNA repair and
cell cycle checkpoint regulation on DNA ... 552 amino acid residues with a molecular mass of
60.9 kDa. OsPol k protein contains a BRCT domain at the
N-terminus and a Pol X domain at the C-terminal region,
similar to the overall domain organization ... were introduced into
OsPol k by a PCR-based method as described previously
[16].
Overexpression of OsPol k protein in
Escherichia coli
The OsPol k protein, which contained a six-histidine
C-terminal...