hormone responsible for raising blood glucose levels
Báo cáo khoa học: Specific biomarkers for stochastic division patterns and starvation-induced quiescence under limited glucose levels in fission yeast docx
Ngày tải lên :
14/03/2014, 23:20
... H
2
DCFDA dye. Cells were cul-
tured for 6 h in 111, 4.4, 1.1 and 0 m
M glucose, or precultivated in 1.1 mM glucose for 16 h prior to the switch to 0 mM glucose medium for
6 h (rightmost image). Micrographs ... 2.2 and 1.7 mm glucose conditions: the
second division doubling time was approximately 7 h
for 2.2 mm glucose and 4–48 h for 1.7 mm glucose.
Nondividing cells were scarce in 2.2 mm glucose, but
plentiful ... that take up
glucose from blood: 4.4 mm glucose is a pre-breakfast
level in human blood. The reduction in cell size might
at least partly explain the short doubling time in low
glucose. The 33%...
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Tài liệu Investigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of the North Fork of the Shenandoah River, Virginia, during Spring of 2007 ppt
Ngày tải lên :
14/02/2014, 08:20
...
individual samples designated for processing for agricultural
pesticides, hormones, pharmaceuticals, and the YES assay.
The procedures used for preparing SPMD samples for
analysis were similar ... others (in press).
Hormones
Four common natural and synthetic hormones were
targeted in this study. Extracts selected for hormone analy-
sis required derivatization of the hormones to facilitate ... with the Friends of the North Fork of the Shenandoah River
Investigation of Organic Chemicals Potentially Responsible
for Mortality and Intersex in Fish of the North Fork of the
Shenandoah River,...
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Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
Ngày tải lên :
18/02/2014, 14:20
... except for
UDP-GlcNAc acyltransferase, responsible for lipid A biosynthesis in
M. catarrhalis, have been identified. By bioinformatics, two late acyltrans-
ferase genes, lpxX and lpxL, responsible for ... m HCl at 80 °C for 18 h.
The FAMEs were recovered into the organic phase by
three-fold extraction with 50% NaCl ⁄ chloroform (1 : 1
v ⁄ v). A new volume of chloroform was used for each of
the ... LpxM is
responsible for the addition of a secondary myristate
(C14:0) chain at the 3Â-position of lipid A [36,37]. In
H. influenzae, the htrB (lpxL) gene product was shown
to be responsible for the...
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Tài liệu Báo cáo khoa học: Is ATP binding responsible for initiating drug translocation by the multidrug transporter ABCG2? docx
Ngày tải lên :
18/02/2014, 18:20
... post-hydrolysis. Therefore, the dissoci-
ation of phosphate and ⁄ or ADP is likely to be responsible for resetting
of the transporter. The data indicate that, like ABCB1 and ABCC1,
the ‘power stroke’ for translocation ... inform on the orientation of the sites,
only their affinity for interaction with drugs.
The data presented here suggest that the ABC-
G2
R482G
isoform undergoes the following sequence of
conformational ... ABCB1, the R482G isoform
also contains multiple sites of interaction for a single
drug (daunomycin), which can manifest as homotropic
allostery [22]. The latter has been observed for the
bacterial...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
Ngày tải lên :
19/02/2014, 02:20
... an
almost 2000-fold decrease in the k
cat
value for dThd,
and a 270-fold decrease for dCyd. The K
m
value was
increased sevenfold for dThd and 49-fold for dCyd.
The large decrease in k
cat
value ... catalytic rate. For the E52D
mutant the K
m
value was approximately the same as
for the wild-type with dThd, whereas k
cat
was 20 000
times lower. This was also the scenario for the E52H
mutant ... ordered with formation of
a ternary complex [1,2]. Pre-steady-state measurements
indicate that either the catalytic step or a preceding
step is rate determining for the overall forward reac-
tion...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
Ngày tải lên :
19/02/2014, 16:20
... pathways for 3-hydroxyanth-
ralinic acid differs from that in the pathway for 4-amino-
3-hydroxybenzoic acid.
The N-terminal amino acid sequence of the purified
enzyme d id not show significant levels ... coupled assay was
identified as 2-hydroxymuconic 6-semialdehyde by GC-MS
analysis. A mechanism for the formation of 2-hydroxy-
muconic 6-semialdehyde via enzymatic deamination and
nonenzymatic decarboxylation ... characterization of the
enzymes is slow [2,4], p robably because the substrate for
the enzyme assay, 2-aminomuconic 6 -semialdehyde, which i s
formed by ring cleavage of 2-aminoph enol, is unstable and is
converted...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
Ngày tải lên :
22/02/2014, 04:20
... & M artin [6], we also n eeded to obtain
information ab out relevant noncoding regions in which
specific and informative mutations are localized.
For these reasons, we d ecided to analyse the ... M/B).
Interestingly, the mutation s pecific for the M allele affects
the same nucleotide as that which is deleted in the F allele,
and shown to be responsible for the internal deletion of 37
amino-acid ... ollowing
three-step cycle which was repeated 35 times: denaturation
for 30 s (or 1 min) at 94 °C, annealing for 30 s (or 2 min) at
47–60 °C, and extension for 30–60 s (or 3 min) at 72 °C,
using the 2400 (or...
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Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
Ngày tải lên :
07/03/2014, 00:20
... determinant responsible for PKA
phosphorylation in vitro
To identify the structural determinants that are respon-
sible for phosphorylation of GIRK1 by PKA, fusion
proteins comprising truncated forms ... mmolặL
)1
glutathione pH 8.0.
Immunoprecipitation
For the immunoprecipitation experiments, Xenopus laevis
oocytes were injected with cRNAs coding for GIRK1 and
GIRK4 (5 ng per oocyte for each RNA) and the KHA2
antisense ... protein
was also demonstrated to be a direct target for PKA-
catalysed phosphorylation [17,22,23]. Despite several
efforts undertaken to identify the responsible structural
determinant on the GIRK1...
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Báo cáo khoa học: Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase l isoform pdf
Ngày tải lên :
07/03/2014, 17:20
... nuclear localization
for the mSMOl isoform (Fig. 4).
Taken together, these results consistently substanti-
ate the hypothesis that these two structural regions are
mandatory for the nuclear localization ... (2005) 30523059 ê 2005 FEBS
Two short protein domains are responsible for the nuclear
localization of the mouse spermine oxidase l isoform
Marzia Bianchi
1
, Roberto Amendola
2
, Rodolfo Federico
1
, ... neuroblastoma cell line, N18TG2, to investi-
gate its subcellular localization. Augmented transcript
levels for each recombinant protein were detected in
transiently transfected neuroblastoma cells, using...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
Ngày tải lên :
08/03/2014, 16:20
... strain Iso1 was V. paradoxus .For
example, the stain Isol was positive for catalase, oxidase and
nitrate reduction but negative of hydrolysis for gelatin and
starch. Therefore, according to all above ... incubated at 37 °C overnight. The transformants
were screened for enzyme activity by adding to each well
10 lL2mgặmL
)1
lysozyme and incubating at 37 °Cfor
30 min. This was followed by the addition ... mea-
surements were performed at various temperatures for 20 min. The
highest activity was taken as 100%. (B) Thermostability of purified
enzyme. The purified enzyme was preincubation for 30 min at various
temperatures....
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Báo cáo khoa học: The gene carD encodes the aldehyde dehydrogenase responsible for neurosporaxanthin biosynthesis in Fusarium fujikuroi potx
Ngày tải lên :
14/03/2014, 22:20
... Wặm
)2
for different times before
mycelia filtration. For carotenoid analysis of the strains
used in the expression experiments, incubations were per-
formed for 7 days in the dark at 22 °C. For ... to light for 15 min, 30 min, 1 h, or 2 h. Relative levels are
referred to the maximal value determined in the wild type in the
light. All data show averages and standard deviations for four mea-
surements ... fujikuroi were formerly investigated in N. crassa (al-1
[20], al-2 [21,22], and cao-2 [23], respectively).
Recently, we identified in this fungus the gene ylo-1
[24], which is responsible for the aldehyde...
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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
Ngày tải lên :
16/03/2014, 04:20
... sequence as being responsible for the pecu-
liar kinetic characteristics of this enzyme, acting as a
negative determinant for malonyl-CoA sensitivity.
Asp17 may account, at least in part, for the high
degree ... the single amino acid variation observed
between pig and human (Asp17 for human and Glu17
for pig) might be responsible for the kinetic charac-
teristics of both CPT1B enzymes. Consequently, ... scale) for IC
50
calculation. The K
m
for carnitine was obtained by assaying
mitochondria in the presence of increasing carnitine concen-
trations: 50–1500 lm for pig CPTIB, and 50–2000 l m for
human...
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Basics for Raising Backyard Chickens potx
Ngày tải lên :
17/03/2014, 10:20
... recommendations are based on common
protein levels for feeds available in most local feed
stores. It is assumed that the finished feed is balanced for
energy, vitamins, and minerals in relation ...
consumption for the number of birds in the
chicken house. This provides a buffer for a short
term water supply in the event of spillage or
leakage. It also offers an opportunity for the
more ... feed
manufacturer.
ã Its OK to let your chickens forage around for
bugs and greens, but always provide them access
to the appropriate type of formulated balanced
feed as well. Totally “free-ranged”...
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Báo cáo Y học: Monitoring the structural consequences of Phe12 fi D-Phe and Leu15 fi Aib substitution in human/rat corticotropin releasing hormone Implications for design of CRH antagonists pdf
Ngày tải lên :
17/03/2014, 10:20
... index,
3
J
HNHa
,andu vs. w plots, Fig. S2) imply a
conformational preference for an extended structure. The
unambiguous conformational preference for helical struc-
ture for the C-terminal 10-peptide is manifested ... values for a-helix structure, while w
angles for Leu14 and Arg16 are considerably close to the
range of values for 3
10
helix. Additionally, Leu14 possess a u
torsion angle almost typical for the ... the backbone conformational freedom [47–49]. This
limitation is imposed by the steric hindrance that arises from
its gem-dimethyl group. The torsion angles for the main-
chain conformation of Aib15...
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