histone chromatin modifications epigenetic control of gene expression
Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx
... induces gyrase expression, in the physiological state topoisomerase I has little control over gyrase gene expression The purported mechanism for such a control of gyrase gene expression is the ... weak the effect of topoisomerase I on supercoiling actually was, can be quanti®ed in terms of the control coef®cient of metabolic control analysis [20,21] With respect to the control of DNA supercoiling ... dependence of gyrase gene expression on DNA supercoiling This prompted us to ask whether this lack of control by topoisomerase I on DNA gyrase expression was due to a low sensitivity of the gyrase...
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Epigenetic control of neuronal activity dependent gene transcription as a basis for long term memory formation
... activity-dependent gene transcription depends on the action of several enzymes that respond to activity to specifically regulate the expression of genes that effectuate downstream functions The ability of epigenetic ... epigenetic mechanisms are now also thought to function in the dynamic regulation of gene expression in the post-mitotic neurons of the adult nervous system26 The four cardinal mechanisms of epigenetics ... 3-dimensional arrangement of chromatin, and 4) histone code: post-translational modifications of histone molecules that package DNA itself27 The present work focuses on the role of chromatin modification...
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Analysis of gene expression
... Labeling of a PCR-amplified probe with DIG as part of in situ RT-PCR indirect detection of gene expression expression standard RT-PCR was performed showing specific amplification of the TNF gene For ... success of both the DNA elution and re-amplification depends on the amount of cDNA generated during the first round of PCR A good way of optimizing the first round PCR is to take advantage of known genes ... intensity of green versus red This gives a measure of the relative levels of the competing mRNAs bound to each spot and so provides information on the relative level of expression of the genes on...
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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
... the control of gene expression As each step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and translation rate is essential for the control of the expression ... activity of both RRMs Finally, the association of ASF ⁄ SF2 with TIAR led us to investigate its role on the expression of reporter gene bearing an ARE in its 3¢ UTR We showed that overexpression of ... might modulate the expression of ARE-containing genes Accordingly, we tested the effect of overexpressing ASF ⁄ SF2 on the expression of Renilla luciferase (Rluc) reporter genes carrying (or...
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Impact of Gene Expression Profiling Tests on Breast Cancer Outcomes potx
... single or multiple gene predictors not involved ‡ in one of the gene expression profile tests of interest: 150 Article does not involve one of the three gene expression tests of interest: 659 No ... between the investigated gene expression signatures (see Glossary, Appendix B) and the actual gene expressionbased tests The gene “signature” is the collection of genes whose expression levels are ... later This cut-off year was established based on the introduction date of the MeSH heading gene expression profiling,” 2000, and the introduction date of the MeSH heading gene expression, ” 1990...
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Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc
... (2005) Analysis of long-lived C elegans daf-2 mutants using serial analysis of gene expression Genome Res 15, 603–615 10 Ryu EJ, Angelastro JM & Greene LA (2005) Analysis of gene expression changes ... number of serial analysis of gene expression tag sequences into 3¢ complementary DNAs Genes Chromosomes Cancer 33, 252–261 33 van den Berg A, van der Leij J & Poppema S (1999) Serial analysis of gene ... gene expression: rapid RT-PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 34 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression...
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Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx
... of gene expression: rapid RT-PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression ... known of the function of mRNAs in human spermatozoa, it was difficult to estimate whether the rest of the genes were related to the function of human spermatozoa, or just retained during spermatogenesis ... (2003) An anatomy of normal and malignant gene expression Proc Natl Acad Sci USA 99, 11287–11292 10 Lee S, Zhou G, Clark T, Chen J, Rowley JD & Wang SM (2001) The pattern of gene expression in human...
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Báo cáo khoa học: Control of transferrin expression by b-amyloid through the CP2 transcription factor pdf
... the control The level of CP2 expression in each group of cells was confirmed by Western blotting, an example of which is shown (bottom panel) Expression of b-tubulin was used as a loading control ... increases compared to the control (upper panel) The expression levels of proteins were assessed by immunoblotting (bottom panels) Expression of b-tubulin was included as a loading control (D) HEK293 ... dysfunction and death, based on studies of the effects of aggregated Ab in culture [19,20] Iron is involved in the generation of ROS by catalyzing the production of OH• from hydrogen peroxide (H2O2)...
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báo cáo hóa học:" Epigenetic control of the ubiquitin carboxyl terminal hydrolase 1 in renal cell carcinoma" doc
... underlying molecular mechanism of the heterogeneity of UCHL1 expression levels The silencing of UCHL1 was discovered by cDNA microarrays and chemical genomic screening of head and neck squamous cell ... contribute to the understanding of the role of a differential UCHL1 expression during tumorigenesis and progression of human cancers as well as in the course of developing therapy resistance ... R, Manoharan M: The emerging role of epigenetics in urological cancers Can J Urol 2007, 14:3535-3541 Feinberg AP: Phenotypic plasticity and the epigenetics of human disease Nature 2007, 447:433-440...
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báo cáo hóa học:" Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs" pot
... feasibility of analyzing changes in gene expression of articular cartilage two weeks after ACL-transection The specific aims of this study were to determine if changes in relevant gene expression ... mix, 0.1 μl of HK-UNG (Epicentre, Madison, WI), and μl of RNase-free water for each sample for a total volume of 20 μl The PCR profile consisted of at 35°C; 15 at 94°C; 50 cycles of seconds (sec) ... powers of the analyses were lower than 0.8, and therefore should be interpreted with caution Gene expression analysis Significant differences (p < 0.05) in gene expression between ACL-X and control...
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Báo cáo hóa học: " Research Article Clustering of Gene Expression Data Based on Shape Similarity" potx
... μxg represents the mean of xg 2.3 Extraction of Shape Information and Time Scaling To extract shape information of time-varying gene expression, the derivative of the expression trajectory is ... optimal number of clusters was arrived at by multiple runs of the algorithm at values of K, 3.7 Validation of E Coli Expression Data Results We validated the results of our tests using Gene Ontology ... vector of time points associated with gene g, zg is the vector of transformed time-series data (from (1)) associated with gene g, and yg is the resulting vector of first differences associated with gene...
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Báo cáo sinh học: "Promoter architecture and the evolvability of gene expression" pdf
... evolvability of gene expression Science 2007, 317:118-121 38 Rando OJ, Verstrepen KJ: Timescales of genetic and epigenetic inheritance Cell 2007, 128:655-668 39 Raser JM, O’Shea EK: Noise in gene expression: ... effects As noted above, expression divergence (the extent to which expression of a gene evolves) correlates with expression responsiveness (the extent to which expression of a gene is changed in response ... mutation in a gene encoding a relevant chromatin regulator, or an upstream gene regulating the activity of the chromatin regulator, could affect transcription of the downstream target gene Unstable...
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Báo cáo y học: "Microarray analysis of gene expression in lupus" ppt
... with effects of both IFN-α/β and IFN-γ on gene expression In addition to this set of IFN-induced genes, gene expression profiles indicating ischemia and myofiber degeneration and regeneration were ... GeneChips Genes showing at least a twofold difference in comparisons of JDM samples with each of two control samples were used to develop a list of differentially expressed genes Ninety-one genes ... clustering of these genes was then performed to identify gene expression patterns among the study samples As in the JDM study, a striking increase in the expression of a group of genes previously...
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Báo cáo y học: "Perspectives and limitations of gene expression profiling in rheumatology: new molecular strategies" doc
... limited number of genes Concordance or divergence of results can therefore be estimated only from the selection of genes published in more detail Up to now, gene expression profiling has given ... Assessment of disease activity including organ involvement or destruction is currently limited to general markers of inflammation or organ function and needs profound improvement On the basis of gene expression ... Discovery of distinctive gene expression profiles in rheumatoid synovium using cDNA microarray technology: evidence for the existence of multiple pathways of tissue destruction and repair Genes Immun...
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Báo cáo y học: "The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanase" docx
... molecules of the target gene to the number of molecules of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene The primer efficiencies for the test genes were the same as those for the GAPDH gene ... Treatment with licofelone had little effect on the level of its gene expression or on the level of protein In contrast, the level of expression of mRNA for ADAMTS-4 was found to be significantly increased ... the downregulating effects of licofelone on the level of mRNA expression/ protein synthesis of 5-LOX could provide an explanation of how the drug can reduce the synthesis of MMP-13 and ADAMTS-4 and...
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Global orchestration of gene expression by the biological clock of cyanobacteria Carl Hirschie Johnson pot
... of the transcription rates of all genes, accounting for the global regulation of gene expression [22] Gene- specific cis-regulatory elements that mediate rhythmic gene expression might therefore ... overexpression, but a significant non-rhythmic basal level remains These results indicate that KaiC (probably as part of a complex) coordinates genome-wide gene expression; the majority of genes ... Circadian orchestration of gene expression in cyanobacteria Genes Dev 1995, 9:1469-1478 Katayama M, Tsinoremas NF, Kondo T, Golden SS: cpmA, a gene involved in an output pathway of the cyanobacterial...
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Báo cáo y học: "A dynamic model of gene expression in monocytes reveals differences in immediate/early response genes between adult and neonatal cells" potx
... collectively highlight the importance of examining gene expression profiles (or related protein expression levels) over time The limits of gene expression profiling as a technique, albeit a very ... measure of the overall similarity of the dynamic behaviour of these samples The greatest temporal changes in gene expression for cord and adult monocytes noted above after 45 of LPS expo- Page of ... accumulation [29] Thus, gene expression profiling should be complemented with other methods in order to maximize there potential In the final analysis, the utility of gene expression profiling will be...
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báo cáo khoa học: " Analysis of gene expression in response to water deficit of chickpea (Cicer arietinum L.) varieties differing in drought tolerance" pps
... their expression profiles in PUSABGD72 The expression profile of individual gene in the cluster is denoted by grey line and the mean expression profile is depicted by pink line The number of genes ... Average expression intensity of the cluster genes was much higher than that of the cluster genes and there was uniformity in the expression of the cluster genes Two ESTs [FL512394 and FL518992] of ... http://www.biomedcentral.com/1471-2229/10/24 Page of 14 Figure Hierarchical clustering analysis of 53 selected genes based on their gene expression patterns Analysis of expression profiles of 53 ESTs (Additional File...
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Báo cáo y học: " 3′-coterminal subgenomic RNAs and putative cis-acting elements of Grapevine leafroll-associated virus 3 reveals ‘unique’ features of gene expression strategy in the genus Ampelovirus" potx
... Discussion Synthesis of 3′-coterminal sgRNAs is one of the genome expression strategies adapted by members of the family Page of 14 Closteroviridae Evidence gathered with viruses in the genera Closterovirus ... the lack of evolutionary conservation in the regulation of gene transcription among monopartite members of the family Closteroviridae The 5′NTR of GLRaV-3 has an unusually long 5′NTR of 737 nt ... used to amplify gene- specific fragments for preparing non-radioactive riboprobes, Table S6: List of primer combinations used to generate gene- specific riboprobes, Table S7: List of gene- specific...
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Báo cáo khoa học: "In vitro dynamics of HIV-1 BF intersubtype recombinants genomic regions involved in the regulation of gene expression" pdf
... viral gene expression, replication, and interaction with the host immune system, eventually leading to remarkable functional consequences [14,15] Transcriptional activation of HIV-1 gene expression ... degree of conservation was observed in all of them but in NFκB and Sp1 sites The HIV-1 promoter contains Sp1 and NF-kB binding sites that regulate gene expression via the recruitment of both ... delicate mechanism of regulation of viral gene expression by compensating any losses or gains in transcription levels This mechanism may play a key role in the adaptation process of recombinant variants...
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