high resolution and targeted profiling of glycerophospholipids and sphingolipids in extracts from drosophila melanogaster
Novel lipidomic approaches to analyse glycerophospholipids and sphingolipids in complex mixtures using mass spectrometry
... Lipids and sensitivity to drugs 109 Chapter High Resolution and Targeted Profiling of Glycerophospholipids and Sphingolipids in Extracts from Drosophila melanogaster 112 5.1 Introduction ... 2.3.2 Non -targeted differential profiling based on ESI-MS and chemometry 46 2.4 Discussion 50 Chapter High Resolution and Targeted Profiling of Glycerophospholipids and Sphingolipids ... kainate induced neuronal injury FASEB J 20(8): 1152-61 xiv 11) Guan XL and Wenk MR (2006) High resolution and targeted profiling of phospholipids and sphingolipids in extracts from Saccharomyces cerevisiae...
- 225
- 1,280
- 0
Báo cáo khoa học: The biochemical properties of the mitochondrial thiamine pyrophosphate carrier from Drosophila melanogaster ppt
... and 4) Samples were taken at the time of induction (lanes and 2) and h later (lanes and 4) The same number of bacteria was analysed in each sample Lane 5, purified DmTpc1p (5 lg) originating from ... Expression of the DmTPC transcripts during development Ethidium bromide staining of the RT-PCR products obtained using specific primers for D melanogaster transcript TPCs and cDNA from Oregon ... serum albumin, ovalbumin, carbonic anhydrase, trypsin inhibitor and lysozyme); lanes 1–4, E coli BL21(DE3) containing the expression vector, without (lanes and 3) and with the coding sequence...
- 10
- 300
- 0
Báo cáo sinh học: " Imaging with isotopes: high resolution and quantitation" potx
... clusters Slodzian’s instruments enabled both high spatial resolution and the high mass resolution necessary for separating isobars at high secondary-ion transmissions Once the instrument and the tracer ... are potentially quite exciting We are also doing experiments where we take thousands of images of a single cell, slowly ‘shaving’ off layers from the top to the bottom, and then reconstruct them ... cells by 15N is really convincing and suggests that MIMS may be highly useful in immunology and cancer research,” says Brad Amos of the MRC Laboratory of Molecular Biology in Cambridge, UK “The paper...
- 5
- 231
- 0
Adiponectin in cattle profiling of molecular weight patterns in different body fluids at different physiological states and assessment of adiponectin’s effects on lymphocytes
... adiponectin receptor and AF allantoic fluid AMPK adenosine monophosphate-activated protein kinase APPL1 adaptor protein containing pleckstrin homology domain, phosphotyrosine binding domain and leucine ... cell surface protein T-cadherin (Hug, 2004) T-cadherin is believed to be one of the AdipoQ binding proteins because of its missing intracellular domain and its lack of expression in hepatocytes ... reproducibility of the semi-quantitative estimation of bovine AdipoQ is shown by an intra-assay variation of 15.7% and an inter-assay variation of 46.5% In addition, repeated freezing and thawing of serum...
- 131
- 350
- 0
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... 1-(b-d-arabinofuranosyl)-cytosine (AraC), 1-(b-d-arabinofuranosyl)-thymine (AraT) and (E)-bromvinyl-2¢-deoxyuridine (BVDU) Mutants of catalytic residues: E52 and R105 It is evident from the kinetic ... binding of dThd and deoxycytidine (dCyd) Results Fig Binding of the substrate dThd at the active site of Dm-dNK [17] Hydrogen-bonding residues are shown E52, Y70, Q81 and R105 were mutated in ... a Mg ion and arginines of the Lid-region stabilize the negative phosphates of this state The most striking effect of mutation of arginine to histidine is an almost 2000-fold decrease in the kcat...
- 10
- 504
- 0
Báo cáo khoa học: Structural studies of nucleoside analog and feedback inhibitor binding to Drosophila melanogaster multisubstrate deoxyribonucleoside kinase doc
... 3¢-azido-2¢,3¢-dideoxythymidine), zalcitabine (ddC, 2¢,3¢-dideoxycytidine) and brivudin [BVDU, (E)-5-(2-bromovinyl)-2¢-deoxyuridine] The kinetic parameters for these are given in Table When discussing the binding and ... binding This is in agreement with the N3 group being somewhat of a hindrance for proper binding but the LID being completely distorted, making P transfer very difficult In yeast thymidylate kinase ... additions BVDU Hanging drops consisted of lL of crystallization solution containing 0.1 m MES, pH 6.5, and 2.5 m Am2SO4 added to lL of enzyme solution containing 20 mgÆmL)1 of protein and 3.7 mm BVDU...
- 10
- 308
- 0
South Africa''''s role in conflict resolution and peacemaking in Africa docx
... diplomacy proved wholly ineffective in promoting dialogue between Kabila and rebels based in Uganda and Rwanda, and in preventing Angola, Namibia and Zimbabwe from sending in troops to prop up his ... rights of states; the need for a strengthening of international law for the purposes of stressing prevention of conflict by way of guidelines, instruments and standards; the attaching of sanctions ... ruling National Party (NP) and the ANC, would be drawn into the business of conflict resolution and peacemaking in Africa, not least because of the iconic status of Nelson Mandela, who during...
- 280
- 506
- 0
Báo cáo hóa học: "Retention of progenitor cell phenotype in otospheres from guinea pig and mouse cochlea" ppt
... the inner ear[14,33,38] Nestin-positive cells expanded in culture from proliferating and floating spherical colonies have been shown to incorporate bromodesoyuridine into the DNA indicating their ... precursor markers as Sox2 and Nestin, and can differentiate in vitro into cells expressing markers of hair cells and supporting cells in vitro[18,31] Culturing organ of Corti progenitor cells ... supervision on cell culturing and immunofluorescence analyses, final image selection and edition, final review of the manuscript RFB: design and coordination of the study Competing interests The authors...
- 10
- 476
- 1
báo cáo khoa học: " Variation at four enzyme loci in natural populations Drosophila melanogaster : factor analyses of genotypic and gametic associations Angeles ALONSO-MORAGA" pdf
... analysis of principal components), with the objective of measuring the factors implicit in the physical variables obtained from each individual In our case the individuals are the populations, and ... carrying Adh allele from F and the gametes carrying Adh and a-Gpdh alleles from figure This s figure correlates with the sequence of intensity of the directional pressures for such loci The influence ... variables are the genotypic and gametic frequencies II Material and methods Two wine cellar populations and two field populations of Drosophila melanogaster from Southern Spain (C6rdoba, latitude 38°)...
- 10
- 229
- 0
evolution of credit and banks in france, from the founding of the bank of france to the present time
... elements of economic progress that were lacking in France In spite of the Revolution, in spite of the anarchy of the Directory and the grave financial disturbances resulting from it, a renewal of industry, ... everything Evolution CHAPTERVI1.-A brief survey of the general course of business during the first half of the nineteenth century-Credit and industrial beginnings Influence o individual directors in ... on the directors in banking statutes which were not of the least use in protecting the interests of the public, and yet were a hindrance to the extension of business In spite of this, these concerns...
- 136
- 1,041
- 0
báo cáo khoa học: "Genomic profiling of plastid DNA variation in the Mediterranean olive tree" pdf
... was observed in lineage E3 (Gué de Constantine 20), while a 225-bp deletion (in the trnQ-rps16 intergenic spacer) was detected in both individuals from South Asia (lineages C1 and C2) In addition, ... microsatellites, indels and CAPS [15] A moderate level of support was also recovered for the clustering of lineages E1 and E2 Only nine informative substitutions were detected in clade Cp-II, three of them ... reported in forest trees and this may also stem from low mutation rate in long-living organisms [22-24] However, higher cpDNA variation has been detected in wild olives than in cultivars, and this...
- 12
- 581
- 0
Báo cáo y học: " Global expression profiling of theophylline response genes in macrophages: evidence of airway anti-inflammatory regulation" ppsx
... supporting the role of theophylline as a regulator of inflammation In this report, interferon regulatory factor (IRF-7) and CD36 were both suppressed by theophylline in macrophages, especially in high ... dosages (Figures and 2A, and Supplemental Table 2) IRF-7 has been studied extensively in viral infection [27] and can induce the gene expressions of interferon and cytokine [28] Interestingly, an overexpression ... important roles in producing broncho-constriction or causing inflammatory changes Theophylline is a weak and non-selective inhibitor of phosphodiesterase (PDE) in airway smooth muscle cells In high doses,...
- 12
- 237
- 0
Báo cáo khoa học: Regulation of calpain B from Drosophila melanogaster by phosphorylation pot
... isoform of protein kinase C, PKCi, enhances both the activity and secretion of l- and m-calpain in human lung cancer cells [7] In the present study, we examined calpain B from Drosophila melanogaster ... subunits of mammalian l- and m-calpain They are composed of an N-terminal regulatory domain (I), a catalytic domain (II), a C2-like domain (III) and a calmodulin-like calcium binding domain (IV) ... consensus recognition motifs of several protein kinases, we initiated the investigation of the phosphorylation of this protein under both in vitro and in vivo conditions In the present study, we...
- 14
- 314
- 0
Báo cáo khoa học: Characterization of a functionally expressed dipeptidyl aminopeptidase III from Drosophila melanogaster doc
... and induced S2 cells in NaCl/Pi containing the aminopeptidase inhibitor bestatine (100 lM), 6.25 nmol of proctolin and 1.75 nmol Arg-Tyr (and Leu-Pro-Thr, not shown) were recovered into the incubation ... met-enkephalin and the insect myotropin proctolin, from which it removes the N-terminal dipeptide, and because it is inhibited by the specific DPP III inhibitor tynorphin [18] The Km values of the recombinant ... is involved in zinc coordination and the catalytic activity of the enzyme Biochemistry 38, 8299–8303 Smyth, M & O’Cuinn, G (1994) Dipeptidyl aminopeptidase activities of guinea-pig brain Int...
- 9
- 357
- 0
Báo cáo khoa học: Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster docx
... panel from the left), or antiDmPCNA1 serum (middle panel) The 29 kDa protein band of DmPCNA2 and the 35 kDa protein band of DmPCNA1 are indicated by arrows Crude extracts from S2 cells expressing ... patterns of binding to chromatin between DmPCNA2 and DmPCNA1 in S2 cells treated with DNA-damaging agents MMS and H2O2 induced a more rapid association of DmPCNA2 with chromatin than of DmPCNA1 ... Chromatin-binding patterns of Drosophila melanogaster proliferating cell nuclear antigen (DmPCNA2) and DmPCNA1 in response to DNA-damaging agents (A) Immunofluorescent analysis of the localization of...
- 12
- 404
- 0
Báo cáo y học: "Comparison of marker gene expression in chondrocytes from patients receiving autologous chondrocyte transplantation versus osteoarthritis patients" pptx
... Competing interests TETEC AG is a tissue engineering company CG and JF have stock holdings in TETEC and have received salaries from the company RS and WKA have received research funding and royalties, ... Fibronectin fragments and blocking antibodies to alpha2beta1 and alpha5beta1 integrins stimulate mitogen-activated protein kinase signaling and increase collagenase (matrix metalloproteinase 13) ... However, controlling the environment within these joints will be of the utmost importance in ensuring the success of ACT in OA patients Hopefully, the development of novel tissue engineering strategies,...
- 10
- 523
- 0
Báo cáo y học: " Protocol for investigating genetic determinants of posttraumatic stress disorder in women from the Nurses'''' Health Study II" pot
... computer screen and is read verbatim to respondents Use of CATI incorporates complex skip patterns into the interview, eliminates post-interview coding errors, and reduces interviewer's inadvertent ... error is detected again in following interviewers, the interviewer is removed from the study Use of highly structured CATI interviews with well-trained carefully monitored interviewers provides ... control procedure: high missing genotype rates (both per individual and per SNP) and deviations from HardyWeinberg genotype proportions are indicative of problems Individuals and/ or SNPs will be...
- 20
- 326
- 0
Báo cáo y học: " Viral particles of the endogenous retrovirus ZAM from Drosophila melanogaster use a pre-existing " potx
... Gag anti-body of ZAM (red) and YL1 antibodies (green) of stage 10 ovarian chambers A) In ovaries of a U-line, the Gag protein of ZAM is detected in follicle cells (red staining), and colocalized ... literature to indicate that retroviral Gag proteins interact with a variety of proteins involved in these pathways Analysis of the role played by the Gag product in ZAM transfer, and its potential interaction ... expressing the distribution of gold anti-gag tagged grains detected in a U-line bearing or not the fs(2)A17 mutation Gold grains were counted in the follicle cells and the oocyte comprised within...
- 9
- 188
- 0
A discourse analysis of urban water management in singapore from 1960 2009
... communication, laying out of industrial estates, expanding electricity and water supplies, and creating the financial institutions that can aid in industrial development”.25 The issue of securing adequate ... stringent urban planning, population resettlement and social engineering In Chapter I explore the discourses surrounding water management of Singapore in the 1980s and 1990s In the 1980s, Singapore ... According to the historical analysis of water supply in colonial Singapore by Jayakumar (1988), wells were the main mode of water supply in Singapore in the 1800s With the expanding trading and...
- 156
- 603
- 0
Consuming more than just water a discourse analysis of urban water management in singapore from 1960 2009
... communication, laying out of industrial estates, expanding electricity and water supplies, and creating the financial institutions that can aid in industrial development”.25 The issue of securing adequate ... stringent urban planning, population resettlement and social engineering In Chapter I explore the discourses surrounding water management of Singapore in the 1980s and 1990s In the 1980s, Singapore ... According to the historical analysis of water supply in colonial Singapore by Jayakumar (1988), wells were the main mode of water supply in Singapore in the 1800s With the expanding trading and...
- 156
- 669
- 0
Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25