hgf sf receptor in human tumors

Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc

Ngày tải lên : 17/03/2014, 23:20

... IL-2 receptors These microdomains contain, among others, a potential regulatory protein of T cell growth, CD48 A pivotal role of cholesterol in maintaining such transient protein assemblies, including ... Endocytosis of interleukin receptors in human T lymphocytes: distinct intracellular localization and fate of the receptor alpha, beta, and gamma chains J Cell Biol 129, 55–64 Lemmon, M.A & Schlessinger, ... Sugamura, K & Waldmann, T.A (1994) Cooperative interactions between the interleukin receptor alpha and beta chains alter the interleukin 2-binding aﬃnity of the receptor subunits Proc Natl Acad Sci USA...

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Báo cáo y học: "Expression of the α7 nicotinic acetylcholine receptor in human lung cells" pdf

Ngày tải lên : 12/08/2014, 18:21

... http://respiratory-research.com/content/6/1/29 Figure showing α7 nicotinic acetylcholine receptor expression in SCLC cell lines Agarose gel Agarose gel showing α7 nicotinic acetylcholine receptor expression in SCLC cell lines cDNA was ... nicotinic acetylcholine receptor protein in non-SCLC cell lines and normal SAE cells as assessed by western Expression Expression of α7 nicotinic acetylcholine receptor protein in non-SCLC cell lines ... α7NCI-H226, NCI-H520, receptor Agarose5 in A-549 adenocarcinoma, NCI-H727 carcinoid, Agarose gel showing α7 nicotinic acetylcholine receptor expression in A-549 adenocarcinoma, NCIH727 carcinoid, and squamous...

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báo cáo hóa học:" Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors" ppt

Ngày tải lên : 20/06/2014, 07:20

... 100×) human ovaries The chicken embryo contains both sphingosine-1 phosphate (ligand for S1P1) and sphingosine kinase; the enzyme responsible for the conversion of sphingosine to sphingosine-phosphate ... follicle development in the laying hen is accompanied by divergent changes in inhibin A, inhibin B, activin A and follistatin production in granulosa and theca layers J Endocrinol 2003, 177:45-55 ... of S1P1 receptor protein expression in hen ovarian tumors (A) An example of a mucinous ovarian tumor with S1P1 + staining associated with surface epithelium (black arrow) and mucin-secreting structures...

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Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafﬁcking pdf

Ngày tải lên : 21/02/2014, 00:20

... by [125I]Streptavidin: (cAMP in receptor- transfected cells – cAMP in control pSG5-transfected cells)/(binding of receptor- transfected cells – binding of the control pSG5transfected cells) The ... measuring the binding of [125I]Streptavidin Specific binding at each point was normalized with reference to specific binding before the incubation at 37 °C (0-min point) to derive the fraction of initial ... containing five amino acids of the extracellular domain and devoid of any tag was not functional [27], while a mutant containing four amino acids of the ectodomain and including a rhodopsine tag...

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Báo cáo khoa học: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages pdf

Ngày tải lên : 06/03/2014, 22:21

... *** *** Visfatin/cyclophilin mRNA Control GW1929 RSG Visfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA A gene [23], was also induced to a similar extent in a dose-dependent ... that visfatin could bind and activate insulin receptors [14] However, the insulinmimetic actions of visfatin are still debated [13] All these data suggest that visfatin might be a player linking ... PPARc-regulated gene in human macrophages Interestingly, PPARc activation enhanced visfatin gene expression in both M1 and M2 human macrophages, but not in murine macrophages or in human adipocytes Finally,...

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Báo cáo khoa học: 15-Deoxy D12,14-prostaglandin J2 suppresses transcription by promoter 3 of the human thromboxane A2 receptor gene through peroxisome proliferator-activated receptor c in human erythroleukemia cells ppt

Ngày tải lên : 07/03/2014, 21:20

... proliferator-activated receptor activators inhibit thrombin-induced endothelin-1 production in human vascular endothelial cells by inhibiting the activator protein-1 signaling pathway Circ Res ... to bind to the radiolabelled PPARc ⁄ RXR probe B Although some weak binding was observed following incubation with either the individual PPARc2 or RXRa proteins, binding was substantially increased ... expression showing no reduction in Prm1 activity or TPa mRNA expression The reduction in TPb mRNA expression in response to 15d-PGJ2 in HEL cells was reﬂected in a corresponding reduction in the overall...

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Báo cáo khoa học: SOX10, in combination with Sp1, regulates the endothelin receptor type B gene in human melanocyte lineage cells pptx

Ngày tải lên : 16/03/2014, 13:20

... binding of SOX10 and Sp1 proteins to the GC1 ⁄ CA2, but were unable to detect the complexes, containing both SOX10 and Sp1 proteins (data not shown) It is conceivable that the in vitro binding ... simultaneous binding of the two proteins to the GC1 ⁄ CA2 SOX10 and Sp1 bind to the EDNRB promoter in vivo To investigate whether SOX10 and ⁄ or Sp1 bind to the EDNRB gene in vivo, chromatin immunoprecipitaion ... SOX10-containing lysates were preincubated for 30 on ice with or without a competitor in the absence of a probe and then incubated with the probe for 30 on ice in 20 lL solution containing mgÆmL)1 bovine...

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Báo cáo khoa học: Downregulation of protease-activated receptor-1 in human lung ﬁbroblasts is speciﬁcally mediated by the prostaglandin E2 receptor EP2 through cAMP elevation and protein kinase A pot

Ngày tải lên : 30/03/2014, 04:20

... AP-2 for binding to the regulatory region of the PAR-1 gene [19,29] Interestingly, in the human alveolar epithelial A549 cell line, Sp1 inhibition, as in human lung ﬁbroblasts, did not in uence ... to an increase in mRNA degradation For this purpose, we determined the half-life of PAR-1 mRNA in the presence of the transcriptional inhibitor actinomycin D The treatment with actinomycin D (7 ... gene expression However, ongoing protein synthesis is required for maintaining the level of PAR-1 on the cell surface Counts Fig Involvement of PKA and Epac in PGE2-induced downregulation of PAR-1...

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báo cáo hóa học:" TRIP-Br2 promotes oncogenesis in nude mice and is frequently overexpressed in multiple human tumors" ppt

Ngày tải lên : 18/06/2014, 15:20

... TRIP-Br for binding to PHD zinc finger- and/or bromodomain-containing proteins, arrests proliferation and induces caspase-3-independent sub-diploidization in cancer cells in vitro [23] In summary, ... protein-protein interactions THD2 consists of one or more PEST signals rich in proline, serine and threonine residues, while THD3 harbors a novel PHD zinc finger- and/or bromodomain-interacting ... carcinoma; 2: Squamous cell lung carcinoma; 3: Breast carcinoma; 4: Gastrointestinal stromal tumors, GIST; 5: Renal cell carcinoma; 6: Ovarian carcinoma; 7: Colon carcinoma; 8: Basal cell carcinoma;...

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Báo cáo hóa học: " Differential inhibition of human cytomegalovirus (HCMV) by toll-like receptor ligands mediated by interferon-beta in human foreskin fibroblasts and cervical tissue" pdf

Ngày tải lên : 20/06/2014, 01:20

... cervicovaginal epithelial cells occurs in the absence of toll-like receptor 4mediated signaling J Immunol 2002, 168(5):2424-2432 van Duin D, Medzhitov R, Shaw AC: Triggering TLR signaling in vaccination ... were maintained in culture medium comprised of Minimum Essential Medium (Gibco, Carlsbad, CA) with 10 mM HEPES, mM L-glutamine, 50 μg/ml gentamycin, 2.5 μg/ml amphotericin B, and 10% fetal bovine ... were cultured in 0.5 ml medium containing Dulbeco's Modified Essential Medium, 24% Ham's nutrient mixture, μg/ml insulin, 50 μg/ml gentamicin, 100 U penicillin/100 μg/ml streptomycin, 20 mM HEPES,...

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Báo cáo khoa học: " Expression of pituitary adenylate cyclase activating polypeptide and its type I receptor mRNAs in human placenta" doc

Ngày tải lên : 07/08/2014, 18:21

... and PAC1 receptor in human placenta at various gestations, during pregnancy In this study, we utilized in situ hybridization to determine the existence of PACAP and PAC1 receptor mRNAs in human ... peptide), showing increasedadenosine 3',5'-monophosphate and interleukin-6 secretion and cell proliferation Endocrinology 1993, 133, 2150-2155 18 Miyata A, Arimura A, Dahl RR, Minamino N, Uehara ... posthybridized in a posthybridization buffer Following a wash in × SSC for 30 min, the sections were then treated with ribonuclease A (50 µg/ ml) at 37oC for 10 min, washed twice in × SSC and × SSC, transferred...

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Báo cáo y học: "The active metabolite of leflunomide, A77 1726, increases the production of IL-1 receptor antagonist in human synovial fibroblasts and articular chondrocytes" ppsx

Ngày tải lên : 09/08/2014, 01:23

... both in vitro and in vivo [11,12] Finally, it has been reported that, at higher concentrations, A77 1726 inhibits different types of receptor and nonreceptor tyrosine kinases that are involved in ... catabolic cytokines, including IL-1, can synergize to induce substantial effects In vivo, it is likely that multiple cytokines present in low amounts act in synergy to induce proinflammatory and ... reported to inhibit different types of receptor and nonreceptor tyrosine kinases [13–15], we assessed whether tyrosine kinase inhibitors would affect IL-1β-induced IL-1Ra production in fibroblasts...

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Báo cáo khoa học: "Coxsackievirus and adenovirus receptor expression in human endometrial adenocarcinoma: possible clinical implications" doc

Ngày tải lên : 09/08/2014, 07:21

... http://www.wjso.com/content/6/1/59 Intense immunostaining for CAR in tumor cells in representative endometrial adenocarcinoma cases (original magnification Figure ×200) Intense immunostaining for CAR in tumor cells in representative ... (high vs low level of CAR expression) and staining intensity (mild vs moderate and intense CAR staining intensity) in patients with endometrial adenocarcinoma did not reveal statistically significant ... activity was developed in 0.5% 3,3'-diaminobenzidine hydrochloride (DAB; Sigma, Saint Louis, MO, USA) in PBS containing 0.03% hydrogen peroxide for Sections were counterstained with Positive (%)...

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Báo cáo khoa học: " Assessment of Epidermal Growth Factor Receptor (EGFR) expression in human meningioma" docx

Ngày tải lên : 09/08/2014, 09:20

... Immunohistochemical staining intensity scores A) Meningiomas stained with anti-EGFR antisera showing negative staining B) 1+ staining C) 2+ staining D) 3+ staining Original magnification for ... (staining percentage (SP) score) and the estimate of the staining intensity (staining intensity (SI) score) When there was multifocal immunoreactivity and a significant difference in staining intensities ... (staining percentage) were estimated by inspection and scored from to 5, (< 20% of the sample exhibiting staining); (2140% of the sample exhibiting staining); (41-60% of the sample exhibiting...

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Báo cáo y học: "Peroxisome proliferator-activated receptor γ1 expression is diminished in human osteoarthritic cartilage and is downregulated by interleukin-1β in articular chondrocytes" doc

Ngày tải lên : 09/08/2014, 10:20

... to increasing concentrations of SB203580 (p38 mitogen-activated protein kinase inhibitor), SP600125 (c-Jun N-terminal kinase inhibitor) and PD98059 (extracellular signal-regulated kinase inhibitor) ... peroxisome proliferator-activated receptor γ inhibits interleukin-1β-induced membraneassociated prostaglandin E2 synthase-1 expression in human synovial fibroblasts by interfering with Egr-1 J Biol Chem ... expression of PPARγ in OA cartilage may, at least in part, be involved in increased expression of inflammatory and catabolic genes, promoting articular inflammation and cartilage degradation In addition,...

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Báo cáo y học: "Activation of proteinase-activated receptor 2 in human osteoarthritic cartilage upregulates catabolic and proinflammatory pathways capable of inducing cartilage degradation: a basic science study" pot

Ngày tải lên : 09/08/2014, 10:22

... signalling pathways involved in PAR-2 activity We showed that PAR-2 is present at increased levels in human OA cartilage and that its level is modulated by the proinflammatory cytokines interleukin ... staining with the avidin-biotin-peroxidase complex method (Vectastain ABC assay) The color was developed with 3,3'diaminobenzidine (DAKO Diagnostics Inc., Mississauga, ON, Canada) containing ... important serine protease systems is the plasminogen activator (PA) plasmin, in which the urokinase PA (uPA) plays a major role [32,33] Interestingly, the uPA/plasmin system, in addition to acting directly...

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Báo cáo y học: "Anti-Inflammatory mechanisms of the proteinaseactivated receptor 2-inhibiting peptide in human synovial cells" ppt

Ngày tải lên : 10/08/2014, 05:21

... (PMSF), 0.8 nM aprotinin,10 nM leupeptin, and mM dithiothreitol Protein concentrations were determined using a Bio-rad assay Page of able to increase COX-2 and MMP-1 protein levels within h in ... cell line as a model to examine trypsin-induced COX-2 and MMP1 expressions Similarly we observed an increased COX-2 protein level by 30 nM trypsin within h of incubation in this cell line (Figure ... transactivating subunit NF-B is normally found in the cytoplasm in an inactivated state by binding to an inhibitor, such as IBa NF-B activation in response to proinflammatory stimuli involves...

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Báo cáo y học: " Differential toll-like receptor 3 (TLR3) expression and apoptotic response to TLR3 agonist in human neuroblastoma cells" docx

Ngày tải lên : 10/08/2014, 10:20

... containing 10% (v/v) heat-inactivated fetal bovine serum (FBS; Invitrogen, Carlsbad, CA), L-glutamine, nonessential amino acids (Invitrogen; 10 mM) and antibiotic-antimycotic (Invitrogen) in a ... immunoreactivity of TLR3 was graded according to the staining intensity as weak (1+), moderate (2+) and strong (3+) staining Moderate or strong staining intensity was considered TLR3-positive The ... specimens were maintained in formaldehydefixed, paraffin-embedded blocks Sections stained with hematoxylin and eosin (H&E) were also reviewed The paraffin sections from specimens were deparaffinized,...

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báo cáo khoa học: "Prognostic significance of STAT3 and phosphorylated STAT3 in human soft tissue tumors - a clinicopathological analysis" ppt

Ngày tải lên : 10/08/2014, 10:21

... After several rinses in phosphate-buffered saline, the sections were incubated in biotinylated secondary antibody for 30 minutes The bound antibodies were detected by a streptavidin-biotin method, ... Vecta Elite ABC staining kit (Vector Laboratories) The slides were rinsed in phosphatebuffered saline, exposed to diaminobenzidine, and counterstained with Mayer’s hematoxylin For the tumor tissues, ... with clinical parameters Histopathological parameters were studied using μm thick paraffin sections stained with Hematoxylin and Eosin and the tumors were broadly classified into benign, intermediate...

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Báo cáo y học: " Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages" pot

Ngày tải lên : 12/08/2014, 11:22

... were used instead of FACS buffer I Intracellular staining of CD68 was done using the IntraStain Reagents (Dako, Carpinteria, CA, USA) according to the manufacturer’s instructions The stained cells ... population in human lungs referred to as lung interstitial macrophages (IM) Studies using primary rat or mouse macrophages suggest that AM are more effective than IM in producing cytokines involved in ... -80°C until use in the multiplex cytokine assay For cytokine measurement, a Milliplex MAP Human Cytokine Kit (Millipore, Billerica, MA, USA) was used, containing the following cytokines: IL1b, IL1ra,...

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