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hershberger bioassay in rats a short term screening assay for anti androgenic properties

Báo cáo sinh học:

Báo cáo sinh học: " A short-term divergent selection for resistance to Teladorsagia circumcincta in Romanov sheep using natural or artificial challenge" pptx

Báo cáo khoa học

... significant and therefore were ignored in Natural or artificial infection with T circumcincta 227 Table I Models of data analysis Information No of data Traits analysed Estimated parameters Model analysed ... grazing animals, the pasture in each paddock was sampled on the first and last day of the grazing period and the larvae were extracted and counted [16] At the same time, grass availability was ... preliminary analysis using the SAS/STAT package [27] To normalise the FEC data, the Univariate procedure indicated that a square root transformation gave better results than a logarithmic transformation,...
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Báo cáo y học:

Báo cáo y học: "Effect of a short-term HAART on SIV load in macaque tissues is dependent on time of initiation and antiviral diffusion" pot

Báo cáo khoa học

... Statistical analysis Statistical analyses were carried out using Stat View software (SAS institute Inc, Cary, North Carolina, USA) Plasma and cell-associated viral load as well as SIV-RNA, total ... Figure Viral dynamics in SIV-infected macaques receiving a short- term HAART just after the viremia peak Among animals infected since 14 days, animals received the AZT/3TC/IDV combination (HAART d14-d28: ... what was observed in macaques treated as early as h pi and macaques treated during early chronic infection Thus, the PVL at day 21 pi was significantly lower in HAART treated animals than in...
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muslims in america a short history oct 2009

muslims in america a short history oct 2009

Vật lý

... Harriet for Liberia, the African American colony in West Africa More than four decades had passed since he had been MUSLIMS IN AMERICA forced to leave his native land Abd al-Rahman’s plan was ... Futa Jalon in Guinea— also the homeland of Abd al-Rahman—Bilali married and had children in the islands He married again and had more children after his arrival in Georgia Though some of Bilali’s ... African Americans Missionary Muhammad Sadiq emphasized his belief that Islam was a universal religion for all people and strongly advocated social equality for African Americans The Ahmadiyya...
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Báo cáo khoa hoc:

Báo cáo khoa hoc:"Food resource allocation patterns in lactating females in a long-term selection experiment for litter size in mice" pot

Báo cáo khoa học

... dam was used as a scaling factor instead) Food intake during lactation relative to mature virgin maternal food intake was calculated as food intake (g/d) divided by the individual estimate of ... from an increase in mammary size, lactating mice and rats experience an increase in liver, heart, lung and gut size to accommodate the large increase in food demands [6,12,17,27,35] In dairy ... the female was estimated as the average of metabolic body weights for all 5-d periods from weaning to 25 d after weaning 2.4 Data analysis   The SAS R program was used for the statistical analysis...
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Báo cáo y học:

Báo cáo y học: "Ipsilateral common iliac artery plus femoral artery clamping for inducing sciatic nerve ischemia/reperfusion injury in rats: a reliable and simple method" doc

Báo cáo khoa học

... participating in drafting the manuscript and statistical analysis GF participating in statistical analysis and study design MRR: Preparing the revised paper and drafting the manuscript SMR participating ... participating in setting up the method ABF participating in setting up the method FAA carried out pathologic assessment ARD participated in the design and cordination of the study All authors read and ... performed in accordance with institutional guidelines for animal care and use and also "Principles of laboratory animal care" were followed The animals weighing 150–200 g were randomly divided into...
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10 steps in developing a strategic social media plan for your business

10 steps in developing a strategic social media plan for your business

Internet Marketing

... track Capture multiple analytics as some metrics may later become a better KPI af ter you have had the experience of measuring and analysing the data Roll out st rat egy Once you have f inalized ... of social media at solving that problem Choose a metric that’s indicative of that goal’s progress and is easy to measure Social media evolves rapidly so your tactics may have to change later T ... ten a great place to start) Discover opport unit ies Social media can be used f or a range of business activities including: Marketing Sales Customer service Research Analysis HR etc Within any...
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The 10 Steps In Developing A Strategic Social Media Plan For Your Business

The 10 Steps In Developing A Strategic Social Media Plan For Your Business

Internet Marketing

... goals and their associated KPIs will vary Speak with and analyse the requirements of each department and work out what level of resource is available f or social media # Def ine goals Analyse any ... buy -in f rom individual departments and management Add timelines and assign tasks Identif y and provide any required training/tools/supports Agree and manage roll out Only sign of f af ter the above ... cost of that tactic Thanks for reading our 10 steps in developing a strategic social media plan for business How would you go about developing a social media strategy? What would you add? Did...
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Tài liệu Keep Your Shop in Tune A Best Management Practices Guide for Automotive Industries docx

Tài liệu Keep Your Shop in Tune A Best Management Practices Guide for Automotive Industries docx

Kĩ thuật Viễn thông

... scrap paper Correctly manifest hazardous wastes Ask for and maintain a Material Safety Data Sheet (MSDS) on each chemical product used in your shop The Oregon Occupational Safety and Health Administration ... work area to a sewer without contacting your local sewerage agency Drain to a single sanitary drain when possible The drain may require an oil/water separator or an oil/grease trap 31 PARKING AND ... potential for site contamination • Join other automotive repair shops in Oregon that are taking pride in maintaining a clean and healthy environment Automotive Industry Wastes The individual sections...
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Báo cáo sinh học:

Báo cáo sinh học: " Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" docx

Điện - Điện tử

... 5'-CTTGCCAACGATTACATTTCCAGRGAYGARCT-3' 5' CTGGCTAACGACTACATCTCCAGRGAYGARCT-3' 5'-GGCAGTTTCAAGGCTGTGAATTTYTTYGARCG-3' 5'-CCGTAAGAAATGGTGGTCCTGACRAAYTGNGG-3' 5'-CCGTAGGCGATGGTCGTCCTAACRAAYTGNGG-3' 5'-TACAAAATACAGCGAGTGATANATRAARCA-3' ... C A T CACA T.G GAGA C T GA GG.GCCCAATT AC GT C.C.GCGG.G.TATTT AG.CAGGCT A T CACA G GAGA C T GACGGAT CCTGGT GCGCGTAA.C A CTGA TCC.CAATGC .A A C .T CAC G.GT GAC.G C T GAAGGTT.ACCTGT CG .A ... 5'-CCGTAGGCGATGGTCGTCCTAACRAAYTGNGG-3' 5'-TACAAAATACAGCGAGTGATANATRAARCA-3' ORF 59 (RFHV/KSHV)7 5'-TGAAAATCCACAGGCATGAT-3' 1The terminal "a" or "b" in the primer name indicates the plus or minus sense of the gene transcription,...
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báo cáo hóa học:

báo cáo hóa học:" Development of a real-time QPCR assay for the detection of RV2 lineage-specific rhadinoviruses in macaques and baboons" pot

Hóa học - Dầu khí

... 5'-CTTGCCAACGATTACATTTCCAGRGAYGARCT-3' 5' CTGGCTAACGACTACATCTCCAGRGAYGARCT-3' 5'-GGCAGTTTCAAGGCTGTGAATTTYTTYGARCG-3' 5'-CCGTAAGAAATGGTGGTCCTGACRAAYTGNGG-3' 5'-CCGTAGGCGATGGTCGTCCTAACRAAYTGNGG-3' 5'-TACAAAATACAGCGAGTGATANATRAARCA-3' ... C A T CACA T.G GAGA C T GA GG.GCCCAATT AC GT C.C.GCGG.G.TATTT AG.CAGGCT A T CACA G GAGA C T GACGGAT CCTGGT GCGCGTAA.C A CTGA TCC.CAATGC .A A C .T CAC G.GT GAC.G C T GAAGGTT.ACCTGT CG .A ... 5'-CCGTAGGCGATGGTCGTCCTAACRAAYTGNGG-3' 5'-TACAAAATACAGCGAGTGATANATRAARCA-3' ORF 59 (RFHV/KSHV)7 5'-TGAAAATCCACAGGCATGAT-3' 1The terminal "a" or "b" in the primer name indicates the plus or minus sense of the gene transcription,...
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báo cáo khoa học:

báo cáo khoa học: " A high-throughput screening system for barley/powdery mildew interactions based on automated analysis of light micrographs" docx

Báo cáo khoa học

... To obtain a stable informational value, this partitioning, training, and testing was performed in terms of 500 different realizations As a result, we obtained a classification accuracy of 95 ± ... partitioned into a training subset and a disjoint test subset [25] We randomly partitioned the data set and used one half for training the classifier and the other half for testing To obtain a ... least risk of overfitting To reach this aim, the feature vector is adapted by nonlinear transformations in advance As an example, consider incorporating the object's perimeter P and area A For...
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Báo cáo y học:

Báo cáo y học: "Assessing the psychometric properties and the perceived usefulness of the BasisRaadsOnderzoek (BARO) as a first-line screening instrument for juvenile offenders" ppt

Báo cáo khoa học

... development of the BARO, a standardized screening instrument was not available for CPB workers, resulting in a vast qualitative and quantitative variety of reports As a result, it was not clear why some ... very much) In case of no information, the examiner can fill in no information In order to provide the investigative magistrate with information on the quality of the data for every domain, whether ... the past, it was neither practice to interview youth and parents separately, nor to interview third party informants The additional travelling time in particular might have increased the total...
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A new term weighting method for text categorization

A new term weighting method for text categorization

Tổng hợp

... discriminating power in the real cases, we gain an insight into a better understanding regarding the basic idea behind tf.rf This chapter provides a detailed qualitative analysis and explanation ... machine learning approach to TC has gained popularity and has eventually become the dominant one In this approach, a general inductive process (also called the learner ) automatically builds a ... a new effective and efficient term weighting method for TC task by using the prior information given by training data set Meanwhile, we also give an analytical explanation of terms’ discriminating...
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Tài liệu Báo cáo khoa học: TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses pdf

Tài liệu Báo cáo khoa học: TransLISA, a novel quantitative, nonradioactive assay for transcription factor DNA-binding analyses pdf

Báo cáo khoa học

... Biotin-TCGACTAGAAGCTTCTAGAAGCTTCTAG AGCTGATCTTCGAAGATCTTCGAAGAT Biotin-TCGACTTCAAGCTTGTACAAGCTTGTAG AGCTGAAGTTCGAACATGTTCGAACATC Biotin-AACGACGGTCGCTCCGCCTGGCT nM Unlabeled HSE DNA-binding activity ... confirm that the assay specifically measures HSF1 DNA-binding activity Analytical range and precision The analytical range of the assay was evaluated using known concentrations of recombinant human HSF1 ... immunoassay, named TransLISA, for measuring the DNA-binding activity of a transcription factor (Fig 1B) This assay is the first homogeneous, nonradioactive assay for rapid and sensitive quantification...
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báo cáo khoa học:

báo cáo khoa học: "A nanoparticle-based immobilization assay for prion-kinetics study" pps

Báo cáo khoa học

... Louis, USA) Hydrogen tetrachloroaurate (III) hydrate (HAuCl4) was obtained from Sigma-Aldrich Inc (Milwaukee, WI) Streptavidin, a strain of Streptomyces avidini was purchased from Sigma-Aldrich Inc ... an essential asset for molecular manipulation for diagnosis purposes, the immobilization of prion protein onto magnetic and gold coated magnetic nanoparticles and the kinetics data obtained in ... signal over a flat substrate and enhances the quantity of adsorbed protein per unit area [16] In this context, metal nanoparticles could serve as potential carriers and/or anchor materials for...
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Báo cáo khoa hoc:

Báo cáo khoa hoc:" Application of a biotin functionalized QD assay for determining available binding sites on electrospun nanofiber membrane" pdf

Báo cáo khoa học

... determined that an assay needed to be developed that was sensitive in the nanogram range to quantitate protein bound to a surface The avidin protein can be attached to a surface and still maintain ... maintain the biotin binding functionality allowing attachment of biotin labeled receptors The strong binding affinity of avidin for biotin (Ka = 1015 M-1) has made it useful for bioanalytical applications ... is anticipated that measurement of binding sites on a planar system using this method would maintain a linear response before a saturation point then plateau The strong fluorescence signal that...
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Báo cáo y học:

Báo cáo y học: " In vitro evaluation of marine-microorganism extracts for anti-viral activity" pot

Báo cáo khoa học

... with antihelmintic, antibacterial, anticoagulant, antidiabetic, antifungal, anti- inflammatory, antimalarial, antiplatelet, antiprotozoal, antituberculosis, and antiviral activities; affecting ... for antiviral activity against four viral isolates that are readily available in this laboratory To properly test these marine extracts for antiviral activity, highly concentrated starting materials ... that have been developed include: a) Ara -A (vidarabine) and Ara-C (cytarabine) (antiviral drugs) derived from the sponge Tethya cripta; b) Okadaic acid and Manoalide (molecular probes) from Dinoflagellate...
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Cell based screening assay for inhibitors of porcine circovirus type2 (PCV2) replication

Cell based screening assay for inhibitors of porcine circovirus type2 (PCV2) replication

Tổng hợp

... 5.04  (GraphPad  Software,  San  Diego  California  USA,  www.graphpad.com).  One‐way  Analysis  of  Variance (ANOVA) with Bonferroni’s post‐test was performed for data obtained for Cell ELISA.  Cell‐Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication  ... (50 µl per well) OPD (SigmaFast OPD, Sigma‐Aldrich). Substrate was incubated in RT for 30, 60,  and 90 minutes in the dark and at each time point, color reaction was assessed by determining  absorbance at 450 nm with Tecan Infinite M200 Plate Reader (Tecan Group Ltd, Switzerland).  ... 0.05, 0.1, 0.5, 1.0, 5.0, and 10 µM, in MEM/5% for 6 hours at 37o C and washed thrice in PBS  before adding fresh medium. Alamar blue was added into each well (10%  v/v) and incubated in the dark at 37o C. Absorbance at 570 nm (referenced at 600 nm) and FI at 590 nm (excitation at ...
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Cell based screening assay for inhibitors of porcine circovirus type2 (PCV2) replication 2

Cell based screening assay for inhibitors of porcine circovirus type2 (PCV2) replication 2

Cao đẳng - Đại học

... Statistical  Parameter  for Use  in Evaluation and Validation of High Throughput Screening Assays. Journal of Biomolecular  Screening 4, 67‐73.  Zhu, Y., Lau, A. , Lau, J., Jia, Q., Karuppannan, A. K., and Kwang, J. (2007). Enhanced replication of  ... negative  value  indicates  that  the  assay results to variable data and not amenable for HTS (Inglese et al., 2007; Sui and Wu, 2007).  Z values for cell‐based  ELISA  was  not  calculated  for ... screening assay would be amenable to HTS. A Z’ factor of > 0.5 indicates amenability for HTS, while a value between 0  and  0.5  indicates  faulty  reagents  or  suboptimal  assay,   and  a negative ...
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