... mutant imitates phosphorylation of Hsp27 by MAPKAP2 kinase [12], and this phosphorylation is induced by different stimuli and stress conditions [19,23,24] Phosphorylation (or mutations) induces ... Hsp27 and formation of small dimers and tetramers that are much more useful for investigation of interactions with denatured actin than large, variably sized oligomers formed by nonphosphorylated ... mixture are indicated for each point Rh of 16 nm under saturation conditions) is much smaller than the corresponding values for native actin filaments or actin aggregates formed upon thermal denaturation...
... forms of sulfate- related expansion Interaction of sulfates with the C-S-H phase Internalsulfateattack 4.8.1 Internalsulfateattack at ambient temperature 4.8.2 Heat- inducedsulfateattackor ... techniques References Sulfateattack 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 Forms of sulfateattack 4.1.1 Manifestations of sulfateattack Source of sulfates in sulfateattack 4.2.1 Internal sources ... reprinted or reproduced or utilised in any form or by any electronic, mechanical, or other means, now known or hereafter invented, including photocopying and recording, or in any information storage or...
... to sulfateattack in concrete and related material ASTM Designation E 150 – Specification for Portland cement ASTM Designation C 452 – Test method for potential expansion of Portland cement mortars ... be required for low permeability or protection against corrosion or freezing and thawing Includes sea water Pozzolan that has been determined by test or service record to improve sulfate resistance ... community regarding the definition of sulfateattack For some, the term means only the process of possible expansion caused by formation of ettringite from external source of sulfate with the C3A...
... attackSulfateattack (chemical attack involving sulfates) Ingress of aggressive chemicals into permeable concrete Permeable concrete Improper processing Reaction of internalor external sulfate ... (also portlandite or CH) In the presence of cement of fly ash, slag, or microsilica, the released portlandite may react with the available silica to form additional C-S-H For additional information ... calcium sulfate compounds of primary interest during sulfateattack in concrete are gypsum (formed during sulfate attack; not gypsum added during cement processing), ettringite, monosulfate,...
... Table 3.1 Factors influencing chemical attack on concrete Factors which accelerate or aggravate attack Factors which mitigate or delay attack (1) High porosity due to i high water absorption ii ... It should be noted that field experience shows ASR to often occur in combination with the DEF- type of internalsulfateattack More detailed information on this phenomenon is given in Chapters ... namely so-called delayed ettringite formation (DEF) , improper curing practices are the most probable cause of damage Learn more about this form of internalsulfateattack in a special section of this...
... removal of the contaminated concrete A typical dilation curve for a mortar sample subjected to heat- inducedinternalsulfateattack (or DEF) is presented in Figure 5.2 As can be seen, the kinetics ... formation) 5.4 CONSEQUENCES OF SULFATEATTACK ON THE MECHANICAL PROPERTIES OF CONCRETE Reports on the consequences of composition -induced internalsulfateattack clearly indicate that this form ... resulting from external sulfateattack has often complicated the work of researchers In order to work on a relatively homogeneous material (for which degradation was more or less uniform throughout its...
... PROTECT CONCRETE AGAINST HEAT- INDUCEDINTERNALSULFATEATTACK Proper mixture design is one way of protecting concrete against degradation by heat- inducedinternalsulfateattack The materials used ... the protection of concrete against composition -induced internalsulfateattack It is therefore recommended to continuously monitor not only the sulfate content of the ground and shipped cement ... proven history of satisfactory performance The lowest possible w/cm is recommended Under proper conditions, to be defined below, there is no evidence showing that regular Portland or blended...
... performance of concrete subjected to sulfateattack Over the years, some authors have elaborated various kinds of empirical equations to describe, for instance, the relationship between sulfateinduced ... P., Sorensen, P and Klinghoffer, O (1996) “HETEK – Chloride penetration into concrete”, Report No 53, The Road Directorate, Copenhagen, Denmark Ouyang, C (1989) “A damage model for sulfateattack ... extent of deterioration of concretes buried in clay for about forty years An alteration zone of about cm was observed in the samples However, the authors mentioned that sulfateattack was probably...
... SLEEPERS: HEAT- INDUCEDINTERNALSULFATEATTACK (DEF) OR ASR? As is the case with external sulfate attack, internalsulfate attack, caused by presence or renewed availability of reactive sulfate ... low-temperature sulfateattack is possible in cases such as over-sulfated cement, sulfates in aggregate, etc As discussed earlier, the actual mechanisms of internalsulfateattackor the differences, ... agrees today that (a) heat- induces sulfateattack is more prevalent in the presence of ASR; (b) there is no credible experimental evidence for “ambient-temperature DEF ; and (c) more data are needed...
... of magnesium sulfate, this effect may mask the sulfateattack Also, a combined sulfate acid attack may occur if the substance responsible for the attack is sulfuric acid or ammonium sulfate All ... prescriptive standards, performance standards describe standardized procedures for a direct assessment of the performance of the cement or concrete under sulfateattack For example, test specimens ... (C3A) in the binder This compound or its reaction product, monosulfate (Afm), are responsible for poor performance in a sulfate environment By reacting with sulfate ions, they yield ettringite...
... vector) or the vector encoding httEx1-25Q-EGFP (25Q EGFP vector), httEx1-72Q-EGFP (72Q EGFP vector) or httEx1-103Q-EGFP (103Q EGFP vector) Cotransfections were performed using the vectors encoding ... pCIneo-EGFP vector (EGFP vector), or the same vector encoding httEx1-25Q-EGFP (25Q EGFP), httEx1-72Q-EGFP (72Q EGFP) or httEx1-103Q-EGFP (103Q EGFP) Cotransfections were performed using the vectors encoding ... transfected with vectors encoding either httEx1-72Q-EGFP (72Q-EGFP) or httEx1-103Q-EGFP (103Q-EGFP) together with pCIneo vector or vectors bearing the Hsp70 ⁄ Hdj-1 (+ Hsp40 + Hsp70) or Hsp27 (+ Hsp27)...
... cho N ão quỵ Spirits - Alcohol: rượu nhiều nguy cho bệnh Tiểu đường N ão quỵ Tóm lại, "3 R for Yes + S for N o" giúp bệnh nhân phòng ngừa N ão quỵ, giảm hiểm nguy, biến chứng dư chứng Mong thay, ... định Bác sĩ Reduce body weight: Giảm ký, béo phì dễ bị cao huyết áp, tiểu đường đột quỵ DON'T: S for No: Salt - bớt muối thức ăn; không nên, không 2.5 gram ngày Saturate fat: kiêng mỡ (ngoại trừ...
... dithiothreitol), incubated with or without mm of the respective nucleotide or phosphate compound for h at °C, and stored for at least weeks at )80 °C before use for kinetic and molecular mass analyses ... tetrameric and dimeric forms in Figs and exhibited low or high catalytic efficiency Therefore, the various incubated hTK1 forms from Figs and were analyzed for their kinetic behavior with dThd, and ... form is more open than hTK1 Therefore, at present, it appears that the adenosine group is very loosely bound to hTK1 In the present work, the part of the phosphate donor that is necessary for...
... Hsp105a or Hsp105b did not significantly affect cell death induced by heat shock, although apoptotic cell death was induced by heat shock treatment (Fig 2) Hsp105a and Hsp105b suppress H2O2 -induced ... mM H2O2 for 0.5 or h (A) orheat shock at 45 °C for h (B) Aliquots (20 lg of protein) of the cell extracts were separated by SDS–PAGE and immunostained using anti-Hsp105, anti-caspase-3 or anti-PARP ... overexpression on the activation of caspase-9 induced by treatment with H2O2 HeLa-tet ⁄ Hsp105 cells were treated with 0.5 mM H2O2 for 0.5 or h (A) orheat shock at 45 °C for h (B) Aliquots (20 lg of protein)...
... cetylpyridinium chloride in H2O The precipitates were solubilized by incubation at 37 °C for 30 Vortexing was performed every 10 during the incubation The absorbance of the solubilized color was measured ... addition of these inhibitors to the complex after calcium activation, but prior to electrophoresis gave the same pattern as without inhibitors (data not shown) The CaCl2 induced conversion of the ... other than Cys75 in the prodomain and induced a conformational change prior to binding to the Cys75, followed by autoactivation It was also shown that if 63 or more amino acids in the prodomain of...
... cells to incorporate neutral red into lysozomes For the neutral red uptake assay, C3H10T1/2 cells (7 · 104 cells per well) in 24-well plates were treated with or without a drug or heat- shocked, ... hsps in mammalian cells (A) C3H10T1/2 cells were incubated with or without IND or NDGA at 37 °C for h, or heat- shocked at 42 °C for h (HS) as a positive control Cell extracts from these cells ... were incubated with or without IND or NDGA at 37 °C for h, and then further incubated at 37 °C for h without the drug As a positive control, cells were heat- shocked at 41 °C for h (HS) Cellular...
... samples were vortexed for min, placed in ice for 15 min, vortexed again for and finally centrifuged at room temperature at 8000 g for 15 Acid extracts were neutralized to pH with NaOH and stored at ... Molecular Cloning: A laboratory manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 14 Kogure, K., Simidu, U & Taga, N (1979) A tentative direct microscopic method for counting living ... twice the mean length of cells before DVC were scored as elongated The proportion of DVC positive cells was corrected by the proportion of elongated cells detected before the DVC method At the same...
... stimulation In this theoretical work, we have provided the first analytical solution for the transmembrane potential in an internal organelle (i.e., mitochondrion) that is induced by a time-varying ... Figure Impact of the presence of internal organelle on ψcell Amplitude (A) and phase (B) of ψcell with the presence of the internal organelle (cycle) or after the organelle was removed from the ... its neighboring cells, without direct physical contact between the two cells [60] This work investigates another important factor that might affect ψcell, i.e., presence of the internal organelle...