gtpase its complexation to yeast gdi and complex crystallization
Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot
... spectra of apo-Tf and its Yb3+ complexes in the highfield region ()1.0 to +0.5 p.p.m) (A) and N-acetyl region (B) Fig Competition between apo-Tf and citrate Changes in percent of Yb bound to Tf (converted ... receptor on gallium-67 uptake and cytotoxicity in lymphoma cell lines Br J Cancer 74, 619–624 19 Roskams, A.J & Connor, J.R (1990) Aluminum access to the brain: a role for transferrin and its ... electrode and calibrated with standard buffers at pH 4.0 and 7.0, respectively Flow cytometry analysis The competitive effect of Yb-Tf species on binding of Fe2-Tf to K562 cells was studied by flow cytometry...
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Characterization the role of kinectin in assembly of translation elongation complex to endoplasmic reticulum and its involvement in organelle motility
... Automated sequencing 59 3.2 Construction of kinectin baits for yeast two-hybrid screening 60 3.2.1 Cloning of Baits A, B and D into BD vector 60 3.2.2 Small-scale transformation of baits into yeast ... have the capacity to bind to both plus and minus end directed motors, and the in vitro and organelle-motor competition binding studies support the concept that a regulated motor complex which contains ... of cell shape, cell motility and cell division (Cole and Lippincott-Schartz, 1995; Garrett and Kapoor, 2003; Sato and Toda, 2004; Scliwa and Honer, 1993; Skoufias and Scholey, 1993; Stukenberg,...
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Development of the Quantitative PCR Method for Candidatus ‘Accumulibacter phosphatis’ and Its Application to Activated Sludge
... (PAO462, PAO651 and PAO846) and an FITC-labeled EUBMIX (EUB338, EUB338 II and EUB338 III) probe (Table 1) The targets of PAOMIX and EUBMIX probe are Candidatus ‘Accumulibacter phosphatis and most eubacteria, ... suitable for routine monitoring or measurement of a large number of samples, and lead to better understanding of the contribution of Candidatus ‘Accumulibacter phosphatis’ to phosphorus removal ... activated sludge was related to those of Candidatus ‘Accumulibacter phosphatis’ Standard curves were constructed from 1.0×103 to 1.0×107 copies of the DNA quantification standard per reaction A linear...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf
... 271) Fig The yeast cytochrome bc1 complex (A) The structure of the dimeric yeast bc1 complex with the redox subunits, cytochrome b, cytochrome c1, and the Rieske ISP colored blue, red and yellow, ... catalytic subunits, cytochrome b, ISP and cytochrome c1 In addition, core proteins and and subunit were reduced to about half of their original levels, while subunits and were reduced to about one ... supernumerary subunits Yeast strains were grown on YPD medium and mitochondrial membranes were analyzed by SDS/PAGE and Western blotting with antibodies to the subunits of the yeast bc1 complex indicated...
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Tài liệu Báo cáo khoa học: "Organizing Encyclopedic Knowledge based on the Web and its Application to Question Answering" ppt
... method (see Sections and 3), and evaluated the quality of the encyclopedia itself Second, we applied the generated encyclopedia to our QA system (see Section 4), and evaluated its performance The ... choice can be performed to improve the coverage to 100% Thus, for each knowledge resource we compared cases without/with random choice, which are denoted “w/o Random” and “w/ Random” in Table 1, respectively ... associated with more complex interrogations, such as ones related to how and why, so as to enhance Webbased natural language understanding Acknowledgments The authors would like to thank NOVA, Inc...
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Tài liệu Báo cáo khoa học: "AUTOMATIC SPEECH RECOGNITION AND ITS APPLICATION TO INFORMATION EXTRACTION" pdf
... value has lead to rapid technology transfer of speech recognition into other research areas and applications Since the variations in speaking style and accent as well as in channel and environment ... between July 1992 • and May 1996, and comprised roughly 500k sentences and 22M words To calculate word ngram language models, we segmented the broadcast-news manuscripts into words by using a ... identification, and a noisy part, and were separately evaluated name extaction, topic c l a s s i f i c a t i o n and The clean part consisted of utterances with no information retrieval background noise, and...
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Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf
... region and b strand 5A of PAI-1 and studied their effect on the transition to latent and substrate forms and on the stabilizing effect of vitronectin, a flexible joint region-binding a cofactor known ... were: wild-type, 48.6 and 65.2 min; K335A, 78.9 and 41.8 min; S53A, 69.4 and 109.9 min; S53A/K335A, 99.2 and 43.5 min; G56S/Q334H, 5.3 and 59.8 min; G56S/Q334H/K335A, 26.5 and 35.1 A summary of ... not identical to that giving an increased rate of latency transition Previously, Triton X-100 was found to induce substrate behaviour in nonglycosylated PAI-1 at and 37 8C [37] and to induce substrate...
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The Savings and Loan Crisis and Its Relationship to Banking pptx
... regulators used supervisory tools and enforcement actions to limit growth and raise capital levels at commercial banks and mutual savings banks But both banks and S&Ls, and their regulators, ... does not have too much influence over its regulators and if the regulators have the ability to hire, train, and retain qualified staff In this regard, the bank regulatory agencies need to remain ... competitors, all eager to lend to developers, had a negative effect on existing commercial banks, on their underwriting standards, and on the quality of their loans Field examiners and bank regulators...
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Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx
... understood The HFE primary sequence exhibits significant homology to major histocompatability complex (MHC) class I molecules and the protein is organized into a1, a2 and a3 structural domains that resemble ... 61129/2004) and the Health Research Board (RP/ 2006/294) to J.V.F and Health Research Board Grant (RP/2005/107) and Science Foundation Ireland (SFI) Investigator Grant (05/IN.3/13859) to MMC The ... reported to co-localize and interact with the type transferrin receptor (TfR1) [5,18] TfR1 mediates the endocytosis of iron-loaded transferrin into acidic endosomes where the iron is released and...
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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
... similarly to its recruitment to GAL1-10 and GAL2 The activator-binding domains of Swi1 and Snf5 are required for galactose-induced expression of SWI ⁄ SNF-dependent genes It was next necessary to determine ... determine whether the activator-binding domains of the SWI ⁄ SNF complex are important for its recruitment to promoters in vivo and to determine whether the Swi1 and Snf5 domains are differentially ... recruitment to promoters M E Ferreira et al tional activators and general transcription factors to target genes [1,16–18] However, the abundance of the SWI ⁄ SNF complex was found to be low [1],...
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Báo cáo khoa học: Identification of yeast aspartyl aminopeptidase gene by purifying and characterizing its product from yeast cells potx
... Mono Q chromatography was used to separate aspartyl aminopeptidase activity into three major peaks (Fig 1) The first and second peaks were pooled as fraction I and II, respectively, and proteins ... peptide and substrate peaks Purification of yeast aspartyl aminopeptidase Yeast cells were grown in YPD medium (10 gÆL)1 yeast extract, 20 gÆL)1 bactopeptone, and 20 gÆL)1 glucose) at 29 °C for 72 ... fractions I and II using a Superose column (s, d) Activity of fractions I and II, respectively (B, C) Results of SDS ⁄ PAGE analysis of fractions I and II Lane numbers correspond to the chromatography...
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Báo cáo khoa học:The principle of flux minimization and its application to estimate stationary fluxes in metabolic networks docx
... oxidative pentose phosphate pathway had to be reversed to maintain the target fluxes Then, the NADPH needed to drive the reactions of the oxidative pathway into a backwards direction and to form hexose ... enzymes, metabolites) can be used to build up mathematical models of the gene-regulatory, signal-transducing and metabolic networks of a cell and to integrate them into whole-cell Ôin silicoÕ models ... effectors, reversible phosphorylation and temporal gene expression These regulatory mechanisms that are operative at the molecular level have evolved during natural evolution and enable the cell to...
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Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot
... dithiothreitol and 10 mM MgCl2) using a motorized mortar and pestle (Torsion Balance Co, Clinton, NJ, USA) Following centrifugation to remove nuclei, yolk platelets, mitochondria (10 000 g, 20 min) and ... yielded both CL and CLAP in a high state of purity However, dissociation of CLP to its subunits (CL and CLAP) required incubation of CLP at pH for at least h at °C prior to chromatography on Mono ... thought to represent destabilizing elements leading to rapid deadenylation and messenger breakdown [47,48] Thus, while CLAP appears to be somewhat refractory to protease degradation, its mRNAs...
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Báo cáo khoa học: "Bayesian Synchronous Tree-Substitution Grammar Induction and its Application to Sentence Compression" pdf
... engineering and is not in general amenable to latent variable problems A simpler alternative to this two step approach is to use a generative model of synchronous derivation and simultaneously segment and ... from nodes in es to nodes in et or We therefore sample in the direction from source to target In particular, we visit every tree pair and each of its source nodes i, and update its alignment by ... of rule c → e, and K is the total number of ways to rewrite c, we now take into account our DP(αc , P0 (· | c)) prior in (1), which, when truncated to a finite grammar, reduces to a K-dimensional...
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Báo cáo khoa học: "A Method for Relating Multiple Newspaper Articles by Using Graphs, and Its Application to Webcasting" pptx
... Kohno, takes a critical attitude toward China, and asks France to understand Japan's position d6: The prime minister of New Zealand asks the French Government not to restart nuclear testing dT: ... reference to its source document, an identifying id, genus and differentia words, and other information on the article The tag < f o l l o w s > is used to denote arcs from the article to related ... the story of the articles For example, the word "state" is the differentia word for dT, and is in its adjacent articles ds, dg, anddlo This means that d7 is a starting point of the new topic...
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Báo cáo khoa học: "Generalized Encoding of Description Spaces and its Application to Typed Feature Structures" potx
... type to the least upper bound, and point the other's type representation to it, and (3) recurse on respective pairs of feature values The frame does not need to be resized, only the types need to ... — we would need to look in a table to see what value to assign it An alternative would be to place that value in the frames for a and b from the beginning But since the value itself must be of ... when it comes to unification, due to appropriateness Nevertheless, unbounded subtyping chains and acquiring new features conspire to force most internal representations of TFSs to perform extra...
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Women’s Employment and Its Relation to Children’s Health and Schooling in Developing Countries: Conceptual Links, Empirical Evidence, and Policies pptx
... increasingly, integration into the global economy Women (like men) shift out of agriculture and into expanding manufacturing, service, and commerce sectors These and concomitant changes in the ... more strongly to community randomized designs; when randomizing over communities, true randomization is more difficult to achieve, and the number of communities must often be large to detect significant ... and control over resources Legislation that guarantees women’s rights to own and inherit property, that makes divorce and child support easier to obtain, and that provides social assistance to...
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THE MINE WATER POLLUTION THREAT TO WATER RESOURCES AND ITS REMEDIATION IN PRACTICE pdf
... Survey, sheets 19 and 25, and parts of 13, 24, 26, 31, 32 (England and Wales) HMSO, London DZOMBAK D.A and MOREL F.M.M 1990 Surface Complexation Modelling Hydrous Ferric Oxide John Wiley and Sons, New ... contributory factor in the decision to abandon the mine in March 1991 Since the abandoned workings of Wheal Jane flooded to river level eight months later (in November 1991) it has proved to be ... lime and settled prior to discharge to the river Serious maintenance problems began to emerge with these ad hoc pump -and- treat facilities around Christmas 1991 As availability of spare parts and...
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Báo cáo khoa học: "Revision Learning and its Application to Part-of-Speech Tagging" pptx
... Japanese and the morphological analyzer has to segment the sentence into words as well as to decide the POS tag of the words So in this section, we describe how to apply revision learning to Japanese ... combines a stochastic model and a binary classifier to achieve higher performance with lower computational cost We applied it to English POS tagging and Japanese morphological analysis, and showed ... i := to l − begin Call the stochastic model to obtain the ordered indexes {n0 , , nk−1 } such that P (cn0 |xi ) ≥ · · · ≥ P (cnk−1 |xi ) for j := to k − begin if cnj = yi then Add xi to the...
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