growth soybean suspension cells and stress treatments
Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx
... demonstrated on child and adult heads [22] and, thus, the epidermal 3D model is a relevant skin cellular model, complementary to isolated cells In this study, we used human cutaneous cells and hRE to test ... detection in human primary epidermal and dermal cells Cells were analysed by flow cytometry using ANX–FITC ⁄ PI The percentage of viable, apoptotic and necrotic cells was determined by quadrant analysis ... of NHDFe cells, exposure to GSM-900 did not induce cell stress in NHDFc cells FEBS Journal 273 (2006) 5491–5507 ª 2006 The Authors Journal compilation ª 2006 FEBS 5493 GSM-900 and cell stress in...
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báo cáo hóa học:" Proliferating and differentiating effects of three different growth factors on pluripotent mesenchymal cells and osteoblast like cells" pdf
... followed by PDGFbb and then BMP-2 The mitogenic effect of the growth factors IGF-I and PDGFbb released from the implant coating on osteoblast like cells and pluripotent mesenchymal cells is also in ... C2C12 cells ML carried out the experiments with the osteoblast like cells TV and GS participated in the study design and coordination and helped to draft the manuscript All authors read and approved ... bone matrix apposition in vitro by local growth factors: a comparison between Insulin-like growth factor I, platelet-derived growth factor and transforming growth factor beta Endocrinology 1990,...
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báo cáo hóa học:" Curcumin induces chemo/radio-sensitization in ovarian cancer cells and curcumin nanoparticles inhibit ovarian cancer cell growth" pot
... cisplatin A2780CP cells were treated as indicated for a total of 48 hrs and protein lysates were collected and analyzed by immunoblotting for caspase and PARP (D) Bands for full length and cleaved ... the understanding regarding the molecular mechanisms involved in curcumin mediated chemo/radio-sensitization in ovarian cancer cells Materials and methods Cell culture and drugs A2780 and A2780CP ... cancer cells (A2780CP) to cisplatin treatment, we designed a curcumin pre-treatment strategy and compared individual treatments (curcumin or cisplatin) to a combination of treatments (curcumin and...
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báo cáo khoa học: " Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco suspension cells" potx
... permeabilize BY-2 cells [47] To enable the use of A thaliana cells in addition to BY-2 and to investigate the regulation of callose synthesis, we wished to establish if Col-0 suspension cultured cells were ... rotary shaker and subcultured every seventh day The cells were harvested for experiments and isolation of membrane fraction during their exponential growth phase (350 – 450 mg fresh weight cells per ... Yellow (BY-2) suspension cells, alamethicin permeabilized the plasma membrane and the inner mitochondrial membrane but not the tonoplast, allowing direct activity measurement of glycolytic and mitochondrial...
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Báo cáo y học: " Differential effects of cigarette smoke on oxidative stress and proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells" potx
... release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells Respir Res 2006, 7:132 Publish with Bio Med Central and every scientist can read your work ... (control) and various concentrations of CSE; a) control, b) CSE (1.0%), c) CSE (2.5%), d) CSE (5.0%), e) CSE (7.5%), f) CSE (10%) for 24 hr The cells were stained with ethidium bromide and acridine ... ethidium bromide and acridine orange and observed under fluorescence microscopy Living cells had normal shaped nuclei with green chromatin Early apoptotic cells have shrunken green nuclei with...
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Báo cáo y học: " Differential effects of cigarette smoke on oxidative stress and proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells" pps
... human and rodent alveolar epithelial cell lines, such as human lung cancer cells (H1299), human lung epithelial cells (H441), murine type II epithelial cells (MLE-15), and rat lung epithelial cells ... on oxidative stress (reduced glutathione and 4-hydroxy-2nonenal), cell toxicity (lactate dehydrogenase release, apoptosis and necrosis) and proinflammatory cytokine release (IL-6 and IL-8) in ... was adjusted to 7.4, and sterile filtered as described above Cell treatments Epithelial cells (H1299, A549, H441, MLE-15 and L2) were seeded at a density of 1.5 million cells in 6-well plates...
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Pleiotropically acting MicroRNA 10b regulates angiogenicity, invasion and growth of tumor cells resembling mesenchymal subgroup of glioblastoma multiforme
... represses growth of glioma-initiating neural stem cells via targeting the epithelial growth factor receptor (EGFR) and plateletderived growth factor receptor alpha (PDGFRA) (21) In addition to miR-7 and ... glioma cells and re-expression of miR-26b diminished proliferation, migration and invasion of glioma cells in vitro (25) Moreover, miR-26b targets ephrin A2 and diminish the capacity of glioma cells ... and miR-125a are down-regulated in GBM and their re-expression in glioma cells coordinately down-regulate podoplanin and consequently inhibit invasion, apoptosis and proliferation of glioma cells...
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Quantification of epidermal growth factor receptor dynamics and interactions in living cells by fluorescent correlation and cross correlation spectroscopy
... cells, such as those made of neurofilament proteins in neural cells and keratins in epithelial cells (Fuchs and Cleveland 1998) α- and β-tubulin form the ~25 nm-diameter-microtubules (Fuchs and ... confirmation and trials to understand their efficacy 1.2.3 ErbB4 ErbB4 shares some ligands with EGFR such as BTC, HBEGF and EPR, shares NRG1 and NRG with ErbB3 and has its specific ligands like NRG3 and ... which in turn phosphorylates and activates Akt, and then regulates cell growth, provides apoptosis resistance to chemotherapy and tumor invasion and migration (Vivanco and Sawyers 2002) Another...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
... blood examination and liver and renal functions, no manifestations of acute and chronic toxic reactions and graft versus host disease, no local swelling at injection sites, and no lower limb ... of acute and chronic toxic reactions and graft versus host disease were observed during and after MSC transplantation Meanwhile, the body temperature, routine blood parameters and liver and renal ... through the ear vein, within more than Necrotic and normal femoral heads, bone marrows, lungs and livers were harvested at 2, and w after transplantation and sectioned followed by observation under...
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Propelling Business Growth With A Secure And Continuous Information Infrastructure
... Growth in complexity and effort Shorten backup and restore times Growth in cost and risk to the business Contain costs Cannot add resources Continuity Defined: Ensuring applications and ... Storage and Management Challenges Information stored on disk arrays ~ 70% IT budget growth ~ - 5% IT environment getting more complex SLA’s continue to expand and tighten Protection and security ... tape and validated Application: restored from tape and validated Data: restored from tape and validated Connectivity: restored and validated Redundancy of data: recover lost transaction and...
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Phonetics and stress exercises
... Graphy : geography, photography,… The stress of nouns, adjectives and verbs having syllables Noun/adj :stress is on the first : export, record, expert Verb : stress is on the second : export, record, ... syllables receive the stress on the second syllables Ate : dictate, locate,… Ize (ise) : surprise, baptize, precise,… Fy, ly : defy, rely, reply,… If having syllables receive the stress on the third ... d.read d.week d.promise d.worked d.city d.spend d.practiced - • - • - - - WORD STRESS The endings which receive the stress: Oo : shampoo, kangaroo, yahoo… (cuckoo) Ee : employee, absentee, trainee,…(committee,...
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The interference of mother tongue as vietnames in learning english souds and stress at high school = ảnh hưởng của tiếng mẹ đẻ đối với việc học âm và trọng âm tiếng anh ở trường phổ thông
... 17 misunderstanding For example, sleep may sound slip and sheep may sound ship Pairs of English vowels such as /i:/ and //, /ổ/ and /e/, /:/ and / /, /:/ and //, /:/ and / /, /u:/ and // are compared ... pronunciation especially sounds and stress is discussed 1.2 Pronunciation Pronunciation consists of three aspects: sound, stress and intonation However, in the thesis, only sounds and stress are focused on ... 2.7 Word Stress 2.7.1 Word Stress in English 2.7.1.1 Definition According to Clarey and Dixson (1963:9), word stress is the emphasis given to a particular syllable within a word We mark stress...
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Therapeutic ultrasound as a potential male contraceptive: power, frequency and temperature required to deplete rat testes of meiotic cells and epididymides of sperm determined using a commercially available system doc
... parameters, and wrote the manuscript; PAD, CMG, RCG, and TSG designed, performed or analyzed in vitro ultrasound experiments, MGO, PAD, GJM and DCS analyzed data and directed Pilot Studies and Study ... necropsies and supervised all animal experiments performed at ILS; EJRS and KGH treated animals, performed necropsies and analyzed sperm All authors participated in experimental design and read and ... dramatic loss of germ cells from testes and that this loss of germ cells was reversible No notable side effects other than infertility were reported during studies with rats, dogs and monkeys [4] This...
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Báo cáo khoa học: WFIKKN1 and WFIKKN2 bind growth factors TGFb1, BMP2 and BMP4 but do not inhibit their signalling activity docx
... and lM) with TGFb1; (D) WFIKKN2 (250 AND 500 nM, and 1, and lM) with TGFb1; (E) WFIKKN1 (400 and 750 nM, and lM) with BMP2; (F) WFIKKN2 (50, 100, 250 and 500 nM) with BMP2; (G) WFIKKN1 (500 and ... 750 nM, and 1.5 lM) with BMP3; (H) WFIKKN2 (100, 250 and 500 nM, and lM) with BMP3; (I) WFIKKN1 (250, 500 and 750 nM, 1, 1.25, 1.5 and lM) with BMP8b; (J) WFIKKN2 (250, 500 and 750 nM, and 1.5 ... nm growth factors for 30 at 37 °C and were added to the cells After 16 h, the cells were washed with NaCl ⁄ Pi and lysed using 50 lL passive lysis buffer from the Biotium luciferase kit, and...
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Complementary and Alternative Treatments in Mental Health Care doc
... London, England Note: The authors have worked to ensure that all information in this book is accurate at the time of publication and consistent with general psychiatric and medical standards, and that ... drug dosages, schedules, and routes of administration is accurate at the time of publication and consistent with standards set by the U.S Food and Drug Administration and the general medical community ... (herbal treatments, physical manipulations) and mind–brain (energy therapies, mindfulness) effects They also challenge us to understand and better use bidirectional effects of body on mind and mind...
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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
... of posttranslational modifications of plasma-derived and recombinant human HCII from CHO cells and their effects on heparin binding MATERIALS AND METHODS Outdated citrated human plasma was a gift ... exclusion assay and the specific HCII productivity (lg per 106 cells per day) was calculated Ó FEBS 2002 Treatment of HCII expressing CHO cells with inhibitors of N-glycosylation and tyrosine sulfation ... carboxamidomethylation and tryptic digestion of HCII from plasma and from recombinant CHO cells One to two nanomoles of the purified protein were reduced, carboxamidomethylated and digested with trypsin;...
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Báo cáo Y học: Characteristics of binding of insulin-like growth factor (IGF)-I and IGF-II analogues to the type 1 IGF receptor determined by BIAcore analysis pptx
... effects, and by allowing 300 s for association and 900 s for dissociation In the case of IGF-I and related analogues, the concentrations used were 12.5, 25, 50, 100, and 200 nM, and for IGF-II and ... for Tissue Growth and Repair (includes Long IGF-I and Gly3IGF-I) Nerve growth factor (NGF) was acquired from Alomone (Jerusalem, Israel) BIAcore CM5 sensor chips, amine coupling kits and surfactant ... penicillin and 100 lgámL)1 streptomycin PC12 cells were detached by trituration, resuspended in complete DMEM, and plated on poly(L-ornithine)-coated plastic culture dishes for 18±24 h before further treatments...
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Environmental Adaptations and Stress Tolerance of Plants in the Era of Climate Change pptx
... Parvaiz Ahmad, and Nicole Geissler Prospects of Halophytes in Understanding and Managing Abiotic Stress Tolerance Vinayak H Lokhande and Penna Suprasanna UV-B Radiation, Its Effects and Defense ... both whole plant and cellular level through osmotic stress in an earlier phase and ionic stress at a later stage of Prospects of Halophytes in Understanding and Managing Abiotic Stress Tolerance ... withstand the stress Chapter describes involvement of different osmolytes, osmoprotectants, and antioxidants during abiotic stress Chapter deals with the role of halophytes in understanding and...
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Growth-oriented Women Entrepreneurs and their Businesses doc
... Economics and Business Administration, Turku, Finland and at the Swedish School of Economics and Business Administration, Helsinki, Finland Her research interests range from entrepreneurship theory and ... debt and equity Sources of growth capital include equity providers (angels, venture capitalists) and debt providers (banks, and other commercial lenders) (Mason and Harrison, 1999; Timmons and ... and Cash note that the growth and prevalence of women entrepreneurs is recognized by researchers and they present an overview of the relative performance of women-owned businesses and their growth...
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