growth and maintenance of bladder cancer cell lines
Mycobacterium bovis, BCG modulation of gene expression in bladder cancer
... Advanced bladder cancer Stages T2 and T3 of cancer development are still localised within the bladder while the T4 stage denotes the advanced stage of bladder cancer, where cancer cells metastasize and ... preparations………………………………………………… 37 2.2.1.1 Growth and Maintenance of bladder cancer cell lines ………………………… 37 2.2.1.2 Preparation of Lyo BCG and live BCG…………………………………………… 37 2.2.1.3 Preparation of FITC labelled BCG………………………………………………… ... Integrin expression and BCG internalization profiles of human bladder cancer cell lines ……………………………………………………………………………… 87 4.1.1 Expression of Integrin α5 on human bladder cancer cells correlates...
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PURIFICATION OF ORGANIC CHEMICALS pot
... 101111 AcOH and diluted with 50ml of H20, (B) 5g KI in lOOml of H20 and (C) 0.3g ninhydrin in lOml of AcOH and 90ml of H20 The spray is prepared by mixing (A) and (B) with 1.5ml of (C) and stored ... impurities are trimethylacetic acid and C9 and C13 acids Dissolve 15g of acid in CCl4 (3OOml) and shake with lOml of 15N aqueous NH3 and the ammonium salt separates and is collected Acid impurities ... refluxed for 2h with 15ml of MeOH and 2ml of 98% H2SO4 (cool when mixing this soh) Pour into 10 volumes of H20 and extract with the minimum volume of CHC13 to give clear separation of phases The extract...
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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc
... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and ... the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ether/acetonitrile/acetone 2:1:1 and 4:3:1 v/v/v) are 0.34 and 0.24, and 0.20 and 0.20, respectively, which...
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PURIFICATION OF LABORATORY CHEMICALS pot
... Salts and organic esters of some inorganic acids Sol vents 5 5 6 6 METHODS OF PURIFICATION SOLVENT EXTRACTION OF REAGENTS AND SOLVENTS AND DISTRIBUTION DISTILLATION Techniques Distillation of liquid ... adsorbents and solvents Preparation and standardisation of alumina Preparation of other adsorbents Silica Diatomaceous earth Charcoal Cellulose Supelco products Flash Chromatography (FC and HPFC) ... REMOVAL OF TRACES OF METALS FROM REAGENTS METAL IMPURITIES DISTILLATION SCAVENGER RESINS AND OTHER SUPPORTS USE OF ION EXCHANGE RESINS PRECIPITATION Removal of lead contaminants Removal of iron...
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purification of carbon nanotubes
... oxidation include air [40–46], a mixture of Cl2, H2O, and HCl [47], a mixture of Ar, O2, and H2O [48–50], a mixture of O2, SF6 and C2H2F4 [51], H2S and O2 [52], and steam [53] High temperature oxidation ... Direct observation Precise content of carbon and metals Diameter, quality and conductivity of SWCNTs Conductivity feature and content of SWCNTs Accurate assessment of F-G on CNTs Elemental contents, ... and metal catalyst was developed Oxidative ions and acid ions dissolved in solution can evenly attack the network of raw samples, and therefore selection of oxidant type and precise control of...
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Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot
... description of the characterization of bacterial GGT and AGT at an enzymatic and gene level Comparison of the amino acid sequences with known enzymes showed the phylogenetic position of 1880 each ... GPT and AGT activities were derived from the single enzymes AT1, AT2 and AT3, respectively (Fig 1) GGT activity was caused by AT1 and AT2, which exhibited 11 and 60 UÆ(mg purified protein))1 of ... purification, suggesting that the four kinds of activity in CFE were derived from only the three enzymes Purified AT1, AT2 and AT3 gave single bands of 44, 42 and 45 kDa on SDS ⁄ PAGE, respectively...
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armarego - purification of laboratory chemicals 5e
... inhalation of vapours and absorption of liquids and low melting solids through the skin In addition to the toxicity of solvents there is also the risk of their flammability and the possibility of eye ... Usually, centrifugation is preferred because of the greater ease and efficiency of separating crystals and mother liquor, and also because of the saving of time and effort, particularly when very small ... mL of 0.2M Na2HPO4 + 61.5 mL of O IM citric acid Or 18 mL of 0.2M NaOAc + 82 mL of 0.2M acetic acid Either 70 mL of 0.2M NaOAc + 30 mL of 0.2M acetic acid Or 51.5 mL of 0.2M Na2HPO4 + 48.5 mL of...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... analyses of HHV-6A- and mock-infected JJHAN cells at dpi Note the CPE, i.e enlargement of cells, in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and ... Infections of JJHAN cells with HHV-6A (U1102) Infections of JJHAN cells with HHV-6A (U1102) A Quantifications of cellular (diamonds) and extracellular (squares) HHV-6A DNA The log10 of the viral ... cytoplasmic and surface proteins gave a positive signal in cell lysates of infected and mock cells (Fig 6, lanes to 4) Next, we analyzed whether the cellular proteins were detected in aliquots of the...
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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
... analyses of HHV-6A- and mock-infected JJHAN cells at dpi Note the CPE, i.e enlargement of cells, in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and ... Infections of JJHAN cells with HHV-6A (U1102) Infections of JJHAN cells with HHV-6A (U1102) A Quantifications of cellular (diamonds) and extracellular (squares) HHV-6A DNA The log10 of the viral ... cytoplasmic and surface proteins gave a positive signal in cell lysates of infected and mock cells (Fig 6, lanes to 4) Next, we analyzed whether the cellular proteins were detected in aliquots of the...
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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc
... interests Luis M Branco, Alex Matschiner, and Darryl B Sampey are co-founders and sole members of the Board of Directors of BioFactura, Inc., and have received salary and other compensation from the company, ... 0.2 g/L of uridine, g/L of glucose, 0.372 mL/L of Dawes Trace 1, 2.14 mL/L of Dawes Trace 2, 0.072 mL/L of Dawes Trace 3, 0.606 mL/L of M calcium chloride dihydrate (CaCl2-2H2O), 0.30 mL/L of 0.43 ... instructions (New England BioLabs) The addition of dithiothreitol (DTT) was necessary to prevent aggregation and precipitation of protein before and during Factor Xa cleavage of LASV GP1-MBP and GP2-MBP...
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Báo cáo hóa học: " Temperature sensitive influenza A virus genome replication results from low thermal stability of polymerase-cRNA complexes" doc
... maximum rate of synthesis prior to that of m- or vRNA and then declines Maximal rates of mRNA synthesis also occur relatively early, before substantial amounts of vRNA are made Amplification of vRNA ... interactions and viral RNA synthesis The size of arrow represents the relative rate of each process At 37°C, polymerase-vRNA complexes are stable and catalyse the synthesis of mRNA and low amounts of cRNA ... m- c and vRNA were quantified by densitometry The ratios of cRNA:mRNA and vRNA:mRNA were calculated and are shown as the fold change (average ± S.D.) in ratios between 31°C and 39°C for cells...
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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx
... was introduced using the sites of restriction endonucleases NdeI and XhoI for cloning with the BCA-kit and a standard curve of BSA in the same buffer in a range of 2-0.005 mg/mL was used Samples ... affinity chromatography and characterization of the enzyme Material and methods Enzymes, chemicals and media Pfu+-polymerase was obtained from Roboclon (Berlin, Germany) and dNTPs from Roth (Karlsruhe, ... Expression of recombinant 2,5-DKCMO in E coli BL21 was performed by cultivation at 37°C to an OD 600 of 0.5, than addition IPTG to a final concentration of 0.1 mM and shifting the culture to 20°C and...
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purification of some heavy metals from paper making
... marina[10],Kandelia candel[11 ]and Aegiceras corniculatum[12] Shuangtai estuary wetland is the final barrier of Shuangtai into Bohai Sea Water quality of Liaohe river has deteriorated, and its heavy ... reed growth Fig.2 removal of Cu Zn Pb Cd at different growth stages of reed Table Variance analysis to removal of Cu at different growth stages of reed Variation source Sum of Squares df Mean Square ... wetland naturally It mainly researches the purification effect of four heavy metals by wetland reed in the different growth periods and parts Furthermore, it provides the theoretic basis and...
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báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps
... end of each hour for SDS-PAGE analysis Cell lysis and protein extraction Bacterial cells were harvested by centrifugation of cell culture at 4000 rpm, 4°C for 10 Supernatant was aspirated off and ... [5, 10, 20 and 40 μl of SiMAC-Nickel bead (100 mg/ml) corresponding to the final concentration of 1, 2, and mg/ml, respectively, and 5, 10, 20, 40 and 80 μl of SDS- PAGE and Western blotting ... yield, and percent of protein recovery and loss were determined for each Nickel-magnetic matrix, each Page 10 of 11 bead concentration and each recombinant protein The sum of the specific band densities...
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Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx
... primary cell cultures of porcine origin The method is both labor intensive and costly, with the additional hazard of requiring the handling of large quantities of infectious virus [21] Economic and ... concentration of about × 106 cells per ml of culture medium (30 ml growing volume) and infected with the recombinant viruses at a multiplicity of infection (MOI) of 2-3 At 72 h postinfection (p.i.), cells ... Construction of recombinant plasmids and recombinant bacmid Genomic DNA was extracted from the cell- cultured strain 20-06 of PPV by the classical phenol-chloroform extraction method and was used...
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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
... a volume of 30 μl of PBS, as follows: PBS containing μg of CT adjuvant (Sigma), 15 μg of p24, 30 μg of RTB/p24, 15 μg of p24 mixed with μg of CT and, 30 μg of RTB/p24 mixed with μg of CT adjuvant ... with immobilized asialofetuin Five mg of asialofetuin were diluted in 0.1 M NaHCO3 and 0.5 M NaCl buffer, pH 8.3 and the solution was employed for immobilization of asialofetuin in Cyanogen bromideactivated-sepharose ... weight mass of the predicted translation products from sequences RTB/p24 and p24 was 57 kDa and 34 kDa respectively E coli expression of RTB/p24 and p24 proteins Purification of RTB/p24 and p24 was...
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PURIFICATION OF SIMPL ANTIBODY AND IMMUNOFLUORESCENCE OF SIMPL SUB-CELLULAR LOCALIZATION IN RESPONSE TO TNFα- AND IL-1
... family of transcription factors that greatly impacts the fate of the cell It can affect the fate of cells in many ways including induction of anti-apoptotic signaling, regulation of cell cycling, and ... cytotoxic T cells as well as memory T cells The helper T cells remain in the lymph tissue where they secrete cytokines and chemokines to manage the activity of B cells, cytotoxic T cells, and macrophages ... capable of inducing an inflammatory response in the endothelial cells, the localization of NF-κB was monitored in control groups of cells Two groups of endothelial cells were incubated with 50 ng of...
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studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene
... hand, PR-S failed to inhibit the growth of Trichoderma viride, Candida albicans, and N crassa, while both zeamatin and osmotin completely inhibited the growth of these three fungi Furthermore, ... chitinases are usually extracellular and show little antifungal activity It seems that extracellular chitinases are involved in generation of signal and transfer of information about infection, ... surface of polymeric substrates (e.g pathogen cell wall), and, hence, allow the hydrolysis of many neighboring chitin strands (Neuhaus, 1999) This could explain the weaker enzymatic activity of class...
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purification of phytase from aspergillus fumigatus
... Silva and Sonia Couri 2009 Purification and characterisation of an extracellular phytase from Aspergillus niger 11T53A9 Brazilian Jf Microbiol 40:795-807 Heinonen, J K and R J Lahti 1981 A new and ... properties, known and potential applications of phytases: An overview Crit Rev Biotechnol 29(2):182-198 Spieck, E and A Lipski 2011 Cultivation, growth physiology, and chemotaxonomy of nitrite-oxidizing ... Preparation of fungi strain and culture medium Aspergillus fumigatus was maintained in PGA medium and incubated in 45°C for days Determine the centration of A fumigatus spore by hemocytometer and adjust...
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THE CONTRIBUTION OF CLAMS ON TIDAL FLAT PURIFICATION CAPACITY
... mixture of chloroform-methanol and then re-extracted into hexane Menaquinones and ubiquinones contained in the crude extract were separated and purified using Sep-Pak® Plus Silica The type and concentration ... abbreviations of the type of quinone are ubiquinone: UQ, menaquinone: MK, plastoquinone: PQ and vitamin K1: K1 RESULTS AND DISCUSSION Diurnal variation of DOC concentration The diurnal variation of DOC ... variation of NH4-N concentration in pore water of tidal flat sediments with and without clams Biodegradability and molecular weight distribution of organic matter Figure shows that more than 80% of...
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Tìm thêm: khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25