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granuphilin rab27a syntaxin 1a and munc18 1 in mouse pancreatic islets

báo cáo hóa học:

báo cáo hóa học: " Pharmacological inhibition of Akt and downstream pathways modulates the expression of COX-2 and mPGES-1 in activated microglia" potx

Toán học

... Nakanishi H: Involvement of COX -1 and up-regulated prostaglandin E synthases in phosphatidylserine liposome-induced prostaglandin E2 production by microglia J Neuroimmunol 2006, 17 2 :11 2 -12 0 13 Coghlan ... rapamycin increases the production of prostaglandins It has been shown that mTOR inhibitors reduced NOS activity and iNOS expression induced by hypoxia and a mixture of cytokines in BV-2 cells and ... LPS-induced COX-2 and mPGES -1 protein synthesis Interestingly, rapamycin, a well-known mTOR inhibitor, increased both COX-2 and mPGES -1 immunoreactivity (Fig 3A-B) The production of PGE2 and...
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Báo cáo toán học:

Báo cáo toán học: "Expressions of EphA2 and EphrinA-1 in early squamous cell cervical carcinomas and their relation to prognosis" pps

Báo cáo khoa học

... (15 ) 11 7 10 0 10 6 92 11 (9) (8) 202 15 18 7 11 15 (7) (27) 17 5 42 15 9 39 16 (9) (7) Ephrin A -1* p‡ 0. 812 Low High (%) 85 78 28 12 (12 ) (9) (18 ) 11 1 45 35 11 (9) (6) 12 (25) 10 4 87 13 (11 ) 13 (13 ) ... radical hysterectomy and lymphadenectomy with or without postoperative irradiation Int J Med Sci 2008, 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Cancer 19 84; 54: 17 14-9 Kamura T, Tsukamoto ... weak cytoplasmic staining for EphA2 and EphrinA -1 was present in 4 /10 (40%) and 6 /10 (60%) of the cases, respectively EphA2 and EphrinA -1 staining were seen in basal, parabasal and middle layers...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc

Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc

Báo cáo khoa học

... M., Yoshihara, K., Singer, A., Valentin, E., Ghomashchi, 13 14 15 16 17 18 19 20 21 22 23 24 25 26 F., Lambeau, G., Gelb, M.H & Kudo, I (20 01) Distinct arachidonate-releasing functions of mammalian ... cationic amino acid residues in their C-terminal regions [ 21, 22] Since the multiple cationic residues in the corresponding C-terminal portions of mouse and human sPLA2-IIAs and rat and human sPLA2-Vs ... which two lysine residues near the C-terminal end (Lys138 and Lys140) were replaced by glutamic acid, and the KE2RS2 mutant, in which two conserved arginine residues (Arg136 and Arg138) were additionally...
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Báo cáo khoa học: Tumor suppressor p16INK4a: Downregulation of galectin-3, an endogenous competitor of the pro-anoikis effector galectin-1, in a pancreatic carcinoma model pptx

Báo cáo khoa học: Tumor suppressor p16INK4a: Downregulation of galectin-3, an endogenous competitor of the pro-anoikis effector galectin-1, in a pancreatic carcinoma model pptx

Báo cáo khoa học

... availability 10 0 10 0 0 10 0 C 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4 Fig p16INK4a restitution inhibits Gal-3 expression (A) Western blot after 1D SDS ⁄ PAGE [15 % gel, proteins blotted onto poly(vinylidene ... Counts 12 0 C 10 0 10 1 10 2 Gal -1 binding (log fluorescence) Fig Gal-3 inhibits Gal -1- stimulated anoikis and Gal -1 binding (A) Determination of Gal -1- stimulated (10 0 lgÆmL )1) anoikis in p16INK4a-restituted ... Gal -1, which involves caspase-8 activation Combining previous results on Gal -1 ⁄ a5b1-integrin co-immunoprecipitation, and the effects of p16INK4a on a5b1integrin, cell-surface glycosylation and...
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Báo cáo y học:

Báo cáo y học: "TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells" pot

Báo cáo khoa học

... protein-Smad1 signaling and osteoblastic differentiation in murine MC3T3-E1 cells J Bone Miner Res 20 01, 16 :17 72 -17 79 22 Kretzschmar M, Doody J, Massague J: Opposing BMP and EGF signalling pathways ... Ando et al References 10 11 12 13 14 15 16 17 18 19 Chicheportiche Y, Bourdon PR, Xu H, Hsu YM, Scott H, Hession C, Garcia I, Browning JL: TWEAK, a new secreted ligand in the tumor necrosis factor ... Okumura K, Yagita H, Ogawa H: Induction of RANTES by TWEAK/Fn14 interaction in human keratinocytes J Invest Dermatol 2004, 12 2 :11 75 -11 79 Xu H, Okamoto A, Ichikawa J, Ando T, Tasaka K, Masuyama K,...
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báo cáo khoa học:

báo cáo khoa học: "Dysregulation of miR-15a and miR-214 in human pancreatic cancer" potx

Báo cáo khoa học

... cultures and clinical samples BxPC-3 human pancreatic cancer cells were maintained in RPMI 16 40 medium containing 10 % fetal bovine serum (FBS; Gibco BRL) 293T cells were maintained in DMEM containing ... measure the expression of seven mature miRNAs (miR15a, miR-27a, miR -10 0, miR -12 5b, miR -1 81a, miR-200a and miR- 214 ) in 10 pancreatic cancer tissues and their adjacent benign tissues These seven mature ... Peschle C, De Maria R: The miR -15 a-miR -16 -1 cluster controls prostate cancer by targeting multiple oncogenic activities Nat Med 2008, 14 (11 ) :12 71- 7 17 Bandi N, Zbinden S, Gugger M, Arnold M, Kocher...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells

Identification of oct4 and sox2 targets in mouse embryonic stem cells

Cao đẳng - Đại học

... Signaling pathways 1. 4 .1. 1 LIF-STAT3 signaling 1. 4 .1. 2 BMP signalling 1. 4.2 Key transcription factors controlling pluripotency 1. 4.2 .1 Oct4 1. 4.2 .1. 1 Oct4 structure 8 11 12 12 12 iii 1. 4.2 .1. 2 ... Sox2 DNA Binding Sites 6 .1 Introduction 11 4 6.2 Results 11 5 6.2 .1 Oct4 and Sox2 co-occupy shared binding sites 11 5 6.2 .1. 1 Comparative location analyses of Oct4 and Sox2 11 5 6.2 .1. 2 Oct4 and Sox2 ... 7.2 .1. 10 Stat3 and Smad1 are Oct4 protein partners 7.2.2 The connection between Stat3, Oct4 and Nanog pathways 14 5 14 6 14 6 7.2.2 .1 Culturing mESCs in defined media containing BMP4 and LIF 14 6...
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Substance p chemokine interaction in mouse pancreatic acinar cells, and its implications in acute pancreatitis

Substance p chemokine interaction in mouse pancreatic acinar cells, and its implications in acute pancreatitis

Cao đẳng - Đại học

... PROTEIN KINASES (MAPKs) 15 1. 11 PHOSPHOLIPASE C 17 1. 12 PROTEIN KINASE C (PKC) 18 1. 13 CALCIUM 20 1. 14 SRC FAMILY KINASES (SFKs) 20 ii 1. 15 ... expression 11 4 4.6 PKCδ is involved in substance P (SP)-induced MCP -1, MIP -1 and MIP-2 secretion 11 6 4.7 Substance P (SP)/NK1R interaction is involved in PKCδ and MEKK1 activation 11 8 5 .1 Substance ... involved in activation of MEKK1, ERK and JNK 10 7 4.4 PKCδ is involved in substance P (SP)-induced NFκB and AP -1 activation 11 2 4.5 PKCδ is involved in substance P (SP)-induced MCP -1, MIP -1 and...
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Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt

Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt

Báo cáo khoa học

... y9-y10, y13, y16-y17 b10-b 11 b6-b15, b23-b25 (2+), y 11- y15 y17, y19 – b 21- b26 (2+), b22-b29 (3+), y19, y 21, y24, y25 (2+) LC-ECD FTICR MS – – – c5-c8 , c 11- c12 Ó FEBS 2003 LC-FTICR MS of pancreatic ... B-chain Insulin 5793.5905 (5793. 610 9) Loss of water c3-c5 from the B-chain C-peptide C-peptide 311 9.5623 ( 311 9.56 21) 313 1.584 ( 313 1.56 21) 21 (A-chain) + 30 (B-chain) 29 31 – c9-c12 Des-(25-29)-C-peptide ... pancreatic islet extract (Fig 1a) The prominent signals in the spectrum originated from products of proinsulin Two insulin variants are expressed in mouse, insulin and [30] Masses corresponding...
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Investigation of the roles of two rac1 effectors phosphatidylinositol 5 kinase 1a and p21 activated kinase 1 in insulin secreting cells

Investigation of the roles of two rac1 effectors phosphatidylinositol 5 kinase 1a and p21 activated kinase 1 in insulin secreting cells

Cao đẳng - Đại học

... PIP5K-Iα in insulin secretion in INS -1 cells 11 1 4 .1. 3 Implication of PIP5K-Iα in PIP2 production in INS -1 cells 11 5 4 .1. 4 Role of PIP5K-Iα in glucose metabolism and membrane potential 11 8 4.2 ... .10 7 4 .1 Roles of PIP5K-Iα in insulin-secreting cells 10 9 4 .1. 1 Involvement of PIP5K-Iα in cell morphology and actin cytoskeleton organization in INS -1 cells 11 0 4 .1. 2 Role ... used insulinsecreting cell lines These includes RIN (70), HIT ( 71) , BRIN-BD 11 (72), MIN6 (73) and INS -1 cells (74) In 19 92, Asfari et al established a highly differentiated insulinsecreting cell...
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Báo cáo y học:

Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"

Y học thưởng thức

... A589G_G590C T100G_C102A T100G_C102A T205G_C206A 18 8 -19 0 18 8 -19 0 18 8 -19 1 18 8 -19 1 RKT RKT RKTT RKTT PKA PKC PKC PKA A568G_C569A_A570C A568G_C569A_A570C A571G_C572A A571G_C572A 18 9 -19 2 KTTS [R/K]X[pS/pT] ... O.P., Meltzer P., Morin P.J., and Weeraratna A.T Claudin -1 overexpression in melanoma is http://www.medsci.org Int J Med Sci 2009, 6 10 11 12 13 14 15 16 17 18 regulated by PKC and contributes to ... Nakamura Y., and Furukawa Y Involvement of claudin -1 in the beta-catenin/Tcf 10 1 signaling pathway and its frequent upregulation in human colorectal cancers Oncol Res 20 01; 12 (11 -12 ): 469-476 19 Dissanayake...
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Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

Báo cáo khoa học

... RPE cells FITC stained CHO-HVEM RPE D Events B 11 4 A C 10 1 10 2 11 9 10 0 10 3 10 4 FITC stained CHO-nectin -1 Events RPE 10 0 10 1 10 2 FITC 10 3 10 4 Fig Expression of HSV -1 gD receptors in RPE cells (A) ... nectin -1 is a major determinant of HSV -1 entry into RPE cells In addition, nectin -1 can in uence cell-to-cell spread of the virions involving membrane fusion Nectin -1 mediates HSV -1 entry into ... 11 6, 12 73 12 81 Spillmann D (20 01) Heparan sulfate: anchor for viral intruders? Biochimie 83, 811 – 817 WuDunn D & Spear PG (19 89) Initial interaction of herpes simplex virus with cells is binding...
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Tài liệu Báo cáo khoa học: Caveolin-1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx

Tài liệu Báo cáo khoa học: Caveolin-1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx

Báo cáo khoa học

... soluble insoluble P2X7R Cav -1 Flotillin -1 T1 B 10 11 12 13 Cav -1 P2X7R Flotillin -1 PDI -Cop TfR T1 Fig (A) Solubility of Caveolin -1, Flo -1 and P2X7 receptor in Triton X -10 0 E10 cells were lysed in ... Rassendren F (19 98) Membrane FEBS Journal 274 (2007) 30 21 3033 ª 2007 The Authors Journal compilation ª 2007 FEBS 30 31 Caveolin -1 and P2X7 expresssion in lung cells 10 11 12 13 14 15 16 17 18 19 20 K ... demonstration of Caveolin -1 and P2X7 distribution in control and in Caveolin -1 shRNA-transfected E10 cells Note the loss of colocalization of Caveolin -1 and P2X7 in Cav -1 shRNA treated cells localization...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

Báo cáo khoa học

... inspection of the region spanning )12 0 nucleotide to )40 nucleotide indicated many potential cis-acting elements, including Sp1, Ets, Stat3, Octamer [35] binding sites and AP1 sites (Fig 3) and ... elements spanning 39 bp in the proximal region of the fgl2 promoter As shown in Fig 4, mutating nucleotides from )87 to )80 (containing Oct -1) , )77 to ) 71 (containing Sp1), )68 to )57 (containing Ets/Stat3) ... homeodomain family proteins bind to this consensus motif and regulate gene transcription in diverse cells through interaction with a variety of partner DNA binding proteins, including Sp1 family...
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Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

Báo cáo khoa học

... 7780 823 376 18 5 14 1 11 9 15 .1 30.6 56.8 62 .1 65.4 10 0 93 85 71 63 2.0 3.8 4 .1 4.3 46 000 24 10 0 9770 8450 19 20 234 10 5 70.6 24.0 10 0 93 12 0 10 0 52 21 18 4.2 3.9 5.0 Purification from 13 g of wet ... 2002 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Structure of B12-dependent glycerol dehydratase (Eur J Biochem 269) 4493 coenzyme B12: X-ray structure of diol dehydratase-adeninylpentylcobalamin ... containing 10 mM imidazole and 300 mM KCl After washing the column with buffer A containing 10 mM imidazole and 300 mM KCl, the enzyme was eluted with buffer A containing 50 10 0 mM imidazole and...
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Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt

Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt

Báo cáo khoa học

... phosphorylated by CKI and CKII Initially two truncated mutants of syntaxin- 1A (1 19 0 and 19 1–265) were subjected to in vitro kinase assays Syntaxin- 1A (1 19 0), but not syntaxin- 1A (19 1–265), is a substrate ... structure of munc18 according to the Ó FEBS 2002 Phosphorylation of syntaxin- 1A by CKI and CKII (Eur J Biochem 269) 911 Fig Phosphorylation of syntaxin- 1A (1 265, 1 19 0, 19 1–265) by CKI and CKII In order ... phosphorylating syntaxin- 1A Figure shows that the cytoplasmic tail of syntaxin- 1A (residues 1 265) is phosphorylated by recombinant CKIa Munc18, a protein which interacts with syntaxin- 1A and regulates...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf

Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf

Báo cáo khoa học

... 2, pUC19 and 10 mM DTT; lane 3, pUC19 and lM FeCl3; lane 4, pUC19, 10 mM DTT, and lM FeCl3; lane 5, pUC19, 10 mM DTT, lM FeCl3, and 50 lgÆmL )1 rBcp2; lane 6, pUC19, 10 mM DTT, lM FeCl3, and 50 ... Aeropyrum pernix APE2278 [10 ] and Pyrococcus horikoshii PH1 217 [11 ,12 ] have A H2O 1- Cys Prx 1- Cys Prx H2O2 Sp OH 722 Identification of the bcp2 gene encoding putative peroxiredoxin The analysis of the ... the N terminus and SR at the C terminus belong to different subunits and condense to form an intersubunit disulfide bond; in atypical 2-Cys, Sp at the N terminus and SR at C terminus, originate from...
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Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx

Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx

Báo cáo khoa học

... were incubated in primary antibodies to PLD diluted : 10 0 in NaCl/Pi containing 2% BSA, 0 .1% Triton X -10 0 and 0 .1% Tween 20 (w/v/v/v) for 16 h Primary antibody-binding sites were visualized by incubation ... Acta 14 36, 10 5 11 5 Serrander, L., Fallman, M & Stendahl, O (19 96) Activation of phospholipase D is an early event in integrin-mediated signalling leading to phagocytosis in human neutrophils In ammation ... range of brefeldin A (0–300 lgÆmL )1) and wortmannin (0 10 0 nM) concentrations (inserts in Fig 3) These results indicate that PI3-kinase-sensitive and brefeldin A-sensitive GEFs (ARF1 or ARF3) play...
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Báo cáo khoa học: The calpain 1–a-actinin interaction Resting complex between the calcium-dependant protease and its target in cytoskeleton doc

Báo cáo khoa học: The calpain 1–a-actinin interaction Resting complex between the calcium-dependant protease and its target in cytoskeleton doc

Báo cáo khoa học

... the interface linking calpain to skeletal muscle a-actinin Colocalization of microcalpain and a-actinin in myogenic cells Calpains and a-actinin were previously located in Z-disks [44], and adhesion ... Calp1 binding site in the C-terminal region of a-actinin [56] situates the protease in the vicinity of titin [57] and CapZ [34], two proteins described as a-actinin partners in the Z-line and ... a-actinin, either in direct contact or in a complex including the two proteins In conclusion, our study proves the interaction between a-actinin and calpain and locates binding motifs within regions...
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