... Nakanishi H: Involvement of COX -1 and up-regulated prostaglandin E synthases in phosphatidylserine liposome-induced prostaglandin E2 production by microglia J Neuroimmunol 2006, 17 2 :11 2 -12 0 13 Coghlan ... rapamycin increases the production of prostaglandins It has been shown that mTOR inhibitors reduced NOS activity and iNOS expression induced by hypoxia and a mixture of cytokines in BV-2 cells and ... LPS-induced COX-2 and mPGES -1 protein synthesis Interestingly, rapamycin, a well-known mTOR inhibitor, increased both COX-2 and mPGES -1 immunoreactivity (Fig 3A-B) The production of PGE2 and...
... M., Yoshihara, K., Singer, A., Valentin, E., Ghomashchi, 13 14 15 16 17 18 19 20 21 22 23 24 25 26 F., Lambeau, G., Gelb, M.H & Kudo, I (20 01) Distinct arachidonate-releasing functions of mammalian ... cationic amino acid residues in their C-terminal regions [ 21, 22] Since the multiple cationic residues in the corresponding C-terminal portions of mouseand human sPLA2-IIAs and rat and human sPLA2-Vs ... which two lysine residues near the C-terminal end (Lys138 and Lys140) were replaced by glutamic acid, and the KE2RS2 mutant, in which two conserved arginine residues (Arg136 and Arg138) were additionally...
... cultures and clinical samples BxPC-3 human pancreatic cancer cells were maintained in RPMI 16 40 medium containing 10 % fetal bovine serum (FBS; Gibco BRL) 293T cells were maintained in DMEM containing ... measure the expression of seven mature miRNAs (miR15a, miR-27a, miR -10 0, miR -12 5b, miR -1 81a, miR-200a and miR- 214 ) in 10 pancreatic cancer tissues and their adjacent benign tissues These seven mature ... Peschle C, De Maria R: The miR -15 a-miR -16 -1 cluster controls prostate cancer by targeting multiple oncogenic activities Nat Med 2008, 14 (11 ) :12 71- 7 17 Bandi N, Zbinden S, Gugger M, Arnold M, Kocher...
... PROTEIN KINASES (MAPKs) 15 1. 11 PHOSPHOLIPASE C 17 1. 12 PROTEIN KINASE C (PKC) 18 1. 13 CALCIUM 20 1. 14 SRC FAMILY KINASES (SFKs) 20 ii 1. 15 ... expression 11 4 4.6 PKCδ is involved in substance P (SP)-induced MCP -1, MIP -1 and MIP-2 secretion 11 6 4.7 Substance P (SP)/NK1R interaction is involved in PKCδ and MEKK1 activation 11 8 5 .1 Substance ... involved in activation of MEKK1, ERK and JNK 10 7 4.4 PKCδ is involved in substance P (SP)-induced NFκB and AP -1 activation 11 2 4.5 PKCδ is involved in substance P (SP)-induced MCP -1, MIP -1 and...
... PIP5K-Iα in insulin secretion in INS -1 cells 11 1 4 .1. 3 Implication of PIP5K-Iα in PIP2 production in INS -1 cells 11 5 4 .1. 4 Role of PIP5K-Iα in glucose metabolism and membrane potential 11 8 4.2 ... .10 7 4 .1 Roles of PIP5K-Iα in insulin-secreting cells 10 9 4 .1. 1 Involvement of PIP5K-Iα in cell morphology and actin cytoskeleton organization in INS -1 cells 11 0 4 .1. 2 Role ... used insulinsecreting cell lines These includes RIN (70), HIT ( 71) , BRIN-BD 11 (72), MIN6 (73) and INS -1 cells (74) In 19 92, Asfari et al established a highly differentiated insulinsecreting cell...
... inspection of the region spanning )12 0 nucleotide to )40 nucleotide indicated many potential cis-acting elements, including Sp1, Ets, Stat3, Octamer [35] binding sites and AP1 sites (Fig 3) and ... elements spanning 39 bp in the proximal region of the fgl2 promoter As shown in Fig 4, mutating nucleotides from )87 to )80 (containing Oct -1) , )77 to ) 71 (containing Sp1), )68 to )57 (containing Ets/Stat3) ... homeodomain family proteins bind to this consensus motif and regulate gene transcription in diverse cells through interaction with a variety of partner DNA binding proteins, including Sp1 family...
... phosphorylated by CKI and CKII Initially two truncated mutants of syntaxin- 1A (1 19 0 and 19 1–265) were subjected to in vitro kinase assays Syntaxin- 1A (1 19 0), but not syntaxin- 1A (19 1–265), is a substrate ... structure of munc18 according to the Ó FEBS 2002 Phosphorylation of syntaxin- 1A by CKI and CKII (Eur J Biochem 269) 911 Fig Phosphorylation of syntaxin- 1A (1 265, 1 19 0, 19 1–265) by CKI and CKII In order ... phosphorylating syntaxin- 1A Figure shows that the cytoplasmic tail of syntaxin- 1A (residues 1 265) is phosphorylated by recombinant CKIa Munc18, a protein which interacts with syntaxin- 1Aand regulates...
... 2, pUC19 and 10 mM DTT; lane 3, pUC19 and lM FeCl3; lane 4, pUC19, 10 mM DTT, and lM FeCl3; lane 5, pUC19, 10 mM DTT, lM FeCl3, and 50 lgÆmL )1 rBcp2; lane 6, pUC19, 10 mM DTT, lM FeCl3, and 50 ... Aeropyrum pernix APE2278 [10 ] and Pyrococcus horikoshii PH1 217 [11 ,12 ] have A H2O 1- Cys Prx 1- Cys Prx H2O2 Sp OH 722 Identification of the bcp2 gene encoding putative peroxiredoxin The analysis of the ... the N terminus and SR at the C terminus belong to different subunits and condense to form an intersubunit disulfide bond; in atypical 2-Cys, Sp at the N terminus and SR at C terminus, originate from...
... were incubated in primary antibodies to PLD diluted : 10 0 in NaCl/Pi containing 2% BSA, 0 .1% Triton X -10 0 and 0 .1% Tween 20 (w/v/v/v) for 16 h Primary antibody-binding sites were visualized by incubation ... Acta 14 36, 10 5 11 5 Serrander, L., Fallman, M & Stendahl, O (19 96) Activation of phospholipase D is an early event in integrin-mediated signalling leading to phagocytosis in human neutrophils In ammation ... range of brefeldin A (0–300 lgÆmL )1) and wortmannin (0 10 0 nM) concentrations (inserts in Fig 3) These results indicate that PI3-kinase-sensitive and brefeldin A-sensitive GEFs (ARF1 or ARF3) play...
... the interface linking calpain to skeletal muscle a-actinin Colocalization of microcalpain and a-actinin in myogenic cells Calpains and a-actinin were previously located in Z-disks [44], and adhesion ... Calp1 binding site in the C-terminal region of a-actinin [56] situates the protease in the vicinity of titin [57] and CapZ [34], two proteins described as a-actinin partners in the Z-line and ... a-actinin, either in direct contact or in a complex including the two proteins In conclusion, our study proves the interaction between a-actinin and calpain and locates binding motifs within regions...