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Recent Advances in Plant Biotechnology

Recent Advances in Plant Biotechnology

Ngày tải lên : 02/10/2012, 10:48
... yeast, and plants) in conjunction with creatine or arginine kinase systems (Sauer and Schlattner, 2004) Beyond such applications in bioprocess engineering, engineering of phosphagen kinase systems ... Blocking Catabolism Plant Vacuole C Fig 2.2 Approaches in metabolic engineering: (1) increase in the total carbon flux at the branch point; (2) decrease in the flux through competitive pathways or introduction ... molecule Fluorescent proteins are increasingly being employed as noninvasive probes in living cells due to their ability to be genetically fused to proteins of interest for investigations of localization,...
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Molecular Farming of Antibodies in Plants

Molecular Farming of Antibodies in Plants

Ngày tải lên : 25/10/2013, 05:20
... in gray and light chains in pink Solid black lines indicate continuation of the polypeptide backbone (simple lines indicate the constant parts of the antibody, curly lines indicate the variable ... of Antibodies in Plants 39 Antigen Antigen Minibody Fab fragment Single chain Fv fragment (scFv) Camelid heavy chain Diabody Large single chain Bispecific scFv Single variable domain scFv-fusion ... subcellular targeting of the protein This is a general method to increase the yield of recombinant proteins because the compartment in which a recombinant protein accumulates in uences its folding, assembly,...
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Regulating Phytonutrient Levels in Plants – Toward Modification of Plant Metabolism for Human Health

Regulating Phytonutrient Levels in Plants – Toward Modification of Plant Metabolism for Human Health

Ngày tải lên : 25/10/2013, 05:20
... others being delphinidin, peonidin, pelargonidin, petunidin, and malvidin Of the flavonols, quercetin, kaempferol, myricetin, and isorhamnetin are common in fruits, quercetin being the predominant ... apigenin, luteolin), flavonols (e.g., quercetin, myricetin), flavanones (e.g., naringenin, hesperidin), catechins or flavanols (e.g., epicatechin, gallocatechin), anthocyanidins (e.g., cyanidin, pelargonidin), ... 12 Regulating Phytonutrient Levels in Plants 311 12.5.4 Metabolic Engineering of the Flavonoid Biosynthetic Pathway in Tomato There is a growing interest in producing food plants with increased...
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Tài liệu In - plant transportation doc

Tài liệu In - plant transportation doc

Ngày tải lên : 18/01/2014, 15:20
... PB-Nr: M06.01.01 In- plant Transportation Issued by Date Hr Bauer Version 00 Page 2/2 ...
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Tài liệu Phytohormones and Abiotic Stress Tolerance in Plants ppt

Tài liệu Phytohormones and Abiotic Stress Tolerance in Plants ppt

Ngày tải lên : 14/02/2014, 12:20
... auxin signaling contributes to induction of immune responses in plants (Bari and Jones 2009) Some of the plant glutathione S-transferases (GSTs) are induced by plant hormones auxins and cytokinins ... receiver domain and a long C-terminal part containing a single-repeat MYB-type DNAbinding domain (Sakai et al 1998) called a GARP domain (Riechmann et al 2000) and the proline- and glutamine-rich ... cytokinins (zeatin and zeatin riboside) are decreased in the xylem in response to drought stress Surprisingly, in the same study, it was found that the level of the aromatic cytokinin 6-benzylaminopurine...
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Tài liệu Báo cáo khoa học: Shaped by the environment – adaptation in plants Meeting report based on the presentations at the FEBS Workshop ‘Adaptation Potential in Plants’ 2009 (Vienna, Austria) pdf

Tài liệu Báo cáo khoa học: Shaped by the environment – adaptation in plants Meeting report based on the presentations at the FEBS Workshop ‘Adaptation Potential in Plants’ 2009 (Vienna, Austria) pdf

Ngày tải lên : 18/02/2014, 11:20
... pathways involved in freezing tolerance in Arabidopsis Freezing tolerance increases in many plants in response to low, non-freezing temperature, a process known as cold acclimation Cold acclimation in ... for thought by providing just such an interdisciplinary forum, in which research results, including novel concepts such as environmentally induced increases in mutation rates in bacteria and a heritable, ... heat-shock proteins in plants Annu Rev Plant Physiol Plant Mol Biol 42, 579– 620 55 Larkindale J & Vierling E (2008) Core genome responses involved in acclimation to high temperature Plant Physiol...
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Tài liệu The Law ( In plan English) for the small Business pdf

Tài liệu The Law ( In plan English) for the small Business pdf

Ngày tải lên : 18/02/2014, 13:20
... Pension Plans 237 Defined Benefit Plans Defined Contribution Plans Designing and Documenting a Plan Employer-Sponsored Plan Investments in a Qualified Plan Chapter ... personally involved in incurring those debts This means that if you are getting involved in a partnership, you should be especially cautious in two areas First, since the involvement of a partner increases ... the business going 8 • The Law (In Plain English)® for Small Business Raising enough cash to buy out the retiring partner, however, could jeopardize the business by forcing the remaining partners...
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Tài liệu Good practices in planning and management of integrated commercial poultry production in South Asia ppt

Tài liệu Good practices in planning and management of integrated commercial poultry production in South Asia ppt

Ngày tải lên : 21/02/2014, 01:20
... Protein Amino acids Lysine Methionine Methionine + Cystine Minerals Calcium Available Phosphorus Manganese Zinc Iron Copper Iodine Selenium Vitamins Vitamin A Vitamin D3 Vitamin E Vitamin K Choline ... sub-clinical bacterial infections and thereby boost performance Examples are tetracyclines, tiamutin, lincomycin, tylosin, erythromycin, colistin, doxycycline, bacitracin, flavomycin, virginiamycin, ... Energy Crude Protein Amino acids Lysine Methionine Minerals Calcium Available Phosphorus Manganese Zinc Iodine Copper Vitamins Vitamin A Vitamin D3 Vitamin E Riboflavin (B2) Vitamin B12 Brooder...
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Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx

Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx

Ngày tải lên : 22/02/2014, 07:20
... due to long range coupling involving vicinal isoprenoid moieties Due to the line broadening, the precision of signal integration is substantially reduced However, within the experimental limits, ... the two competing deprotonation paths is illustrated in Fig for the predominant ES complex A of a sample of IPP carrying a 13 C label in its methyl group; a similar scheme involving a less stable ... by Kleinig & Beyer [37] Inner coronae of N pseudonarcissus (80 g) were homogenized in 250 mL of 67 mM Tris/HCl, pH 7.5, containing mM MgCl2, mM dithioerythritol, mM EDTA, 0.2% (w/v) polyvinylpyrrolidone...
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Uptake of organic chemicals in plants Human exposure assessment ppt

Uptake of organic chemicals in plants Human exposure assessment ppt

Ngày tải lên : 05/03/2014, 20:20
... applied in numerous studies It is well suited for determining distribution constants between environmental media and water, and for the determination of protein-binding affinities (Heringa and ... modelling the uptake of organic compounds into plants any easier However, combining models and experiments gain a lot of insight into the topic, and models can be used to help design and interpret ... coating SPME Sampling Kinetics SPME sampling kinetics were determined for different sample matrices in both static and shaken mode (Table 2) The sampling rates were on average 36% higher in the...
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Báo cáo khoa học: Arabidopsis thaliana CYP77A4 is the first cytochrome P450 able to catalyze the epoxidation of free fatty acids in plants potx

Báo cáo khoa học: Arabidopsis thaliana CYP77A4 is the first cytochrome P450 able to catalyze the epoxidation of free fatty acids in plants potx

Ngày tải lên : 07/03/2014, 03:20
... monomer into the cutin In this context, it is interesting to note that inhibition studies allowed LeQueu et al [49] to demonstrate the involvement of a peroxygenase in the formation of cutin of ... generated in incubations of linoleic acid with microsomes from yeast expressing CYP77A4 Microsomes were incubated with 100 lM [1-14C]linoleic acid in the absence (A) or presence (B) of NADPH Incubations ... encoding a fatty acid x-hydroxylase, a putative epoxide hydrolase, several genes encoding enzymes involved in the synthesis of fatty acids in plastids, including the stearoyl acyl carrier protein...
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Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx

Báo cáo khoa học: Plant DNA polymerase k, a DNA repair enzyme that functions in plant meristematic and meiotic tissues docx

Ngày tải lên : 07/03/2014, 15:20
... may be important for plant cells On the other hand, the N-terminal BRCT domain proposed to mediate protein–protein interactions involved in DNA repair and cell cycle checkpoint regulation on DNA ... 552 amino acid residues with a molecular mass of 60.9 kDa OsPol k protein contains a BRCT domain at the N-terminus and a Pol X domain at the C-terminal region, similar to the overall domain organization ... were introduced into OsPol k by a PCR-based method as described previously [16] Overexpression of OsPol k protein in Escherichia coli The OsPol k protein, which contained a six-histidine C-terminal...
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New techniques in plant biotechnology

New techniques in plant biotechnology

Ngày tải lên : 13/03/2014, 21:56
... the aim of integrating the mutation introduced into the gene construct into the plant-based gene In this way, a plant-based gene can also be target activated via insertion In combination with ... grafting on GM rootstock Grafting is a technique that has been used for centuries in plant breeding In grafting, the bud-bearing part (the graft) of a plant is grafted onto the rootbearing part ... changes in the function of genes that cannot be reversed by changing the DNA sequence For the breeding industry, epigenetics is interesting because it offers the possibility of inducing effects in...
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Báo cáo khoa học: Sugar signalling and antioxidant network connections in plant cells pptx

Báo cáo khoa học: Sugar signalling and antioxidant network connections in plant cells pptx

Ngày tải lên : 15/03/2014, 11:20
... metabolized Invertases (INVs) can in uence plant growth and development either directly (e.g by in uencing source–sink relationships or by acting as a true regulatory protein) or indirectly via ... Cytokinins, on the other hand, can delay plant senescence, and studies with the AtHXK1 mutant glucose insensitive show that sugars and cytokinins work antagonistically [19] Interestingly, cytokinin-induced ... contributing to increasing antioxidant abilities [17] and assisting in recycling sugars from sugar radicals (Fig 1) Cellular NDP-sugar concentrations Sugar signalling and antioxidant networks in plants...
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Báo cáo khoa học: Improvement of ecdysone receptor gene switch for applications in plants: Locusta migratoria retinoid X receptor (LmRXR) mutagenesis and optimization of translation start site pptx

Báo cáo khoa học: Improvement of ecdysone receptor gene switch for applications in plants: Locusta migratoria retinoid X receptor (LmRXR) mutagenesis and optimization of translation start site pptx

Ngày tải lên : 15/03/2014, 23:20
... GAL4 DNA-binding domain with CfEcR ligand-binding domain cloned under the control of the 35S promoter in the pKYLX80 vector A fusion of VP16 activation domain and LmRXR ligand-binding domain cloned ... Kozak sequence Interestingly, an increase in the ligandinduced activity of this switch containing the improved Kozak sequence was not accompanied by an increase in background activity in the absence ... containing wild-type LmRXR and CH9, as a partner of CfEcR, in inducing reporter gene expression in tobacco protoplasts The results observed in protoplasts were confirmed in Arabidopsis transgenic plants...
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Báo cáo khoa học: Photosynthetic acclimation: State transitions and adjustment of photosystem stoichiometry – functional relationships between short-term and long-term light quality acclimation in plants ppt

Báo cáo khoa học: Photosynthetic acclimation: State transitions and adjustment of photosystem stoichiometry – functional relationships between short-term and long-term light quality acclimation in plants ppt

Ngày tải lên : 16/03/2014, 06:20
... determinants include the geographical latitude of the ecosystem, the appearance of clouds and the leaf movement by wind Biotic in uences depend mainly on the individual position of a plant within ... phosphorylations were not found in stn7 ⁄ stn8 double mutants [40], pointing to a possible interaction or cross-talk between both kinases in the wild-type plant (Fig 3) A study using MS reported high specificities ... wild-type plant By contrast, the double mutant stn7 ⁄ stn8 showed much more reduction in seed production, indicating synergistic effects when both kinases are lacking [9] Additional interesting results...
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Báo cáo khoa học: Improvement of a monopartite ecdysone receptor gene switch and demonstration of its utility in regulation of transgene expression in plants pdf

Báo cáo khoa học: Improvement of a monopartite ecdysone receptor gene switch and demonstration of its utility in regulation of transgene expression in plants pdf

Ngày tải lên : 16/03/2014, 06:20
... Arabidopsis plants were selected by germinating the seeds collected from the in ltrated plants on a medium containing 50 mgÆL)1 kanamycin Resistant T1 plants surviving on kanamycin-containing medium ... foreign genes in plants Gene 61, 1–11 Tavva VS, Collins GB & Dinkins RD (2006) Targeted overexpression of the Escherichia coli MinC protein in higher plants results in abnormal chloroplasts Plant Cell ... expression in the absence of methoxyfenozide was indistinguishable from the background readings detected in the transgenic plants that were transformed with a two-hybrid gene switch containing wild-type...
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Báo cáo khoa học: Evidence for the presence of ferritin in plant mitochondria pdf

Báo cáo khoa học: Evidence for the presence of ferritin in plant mitochondria pdf

Ngày tải lên : 16/03/2014, 18:20
... bovine serum albumin, 20% (v/v) normal goat serum in 0.1 M Trisbuffered saline (pH 7.4), and then incubated for h in Trisbuffered saline (pH 7.4) containing PAAF (diluted : 5), 1% (w/v) bovine ... of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 27 Møller, I.M & Lin, W (1986) Membrane-bound NAD(P)H dehydrogenases in higher plant cells Ann Rev Plant Physiol ... pea seed proteins (CP, control proteins); molecular mass of protein standards is indicated in kDa (Std) (B) Immunoblotting of the same proteins with polyclonal antibody against ferritin (PAAF) (C)...
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Báo cáo khoa học: Regulation of pyruvate dehydrogenase complex activity in plant cells ppt

Báo cáo khoa học: Regulation of pyruvate dehydrogenase complex activity in plant cells ppt

Ngày tải lên : 17/03/2014, 09:20
... expression in flowers might instead reflect mRNAs present in the pollen Mitochondrial PDC Product inhibition As with their mammalian and microbial counterparts, plant PDCs employ a multisite ping-pong kinetic ... flanking sequences are conserved in mammalian E1a primary sequences Stoichiometric phosphorylation of any individual site resulted in total inactivation, but indicated that there were differences in ... membrane expansion and remodeling In most instances, there is coordinate expression of the E1 and E2 components The transient accumulation of mRNA preceding changes in protein levels and catalytic...
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