germ cell tumors gcts
Báo cáo khoa học: In vivo RNA interference in oyster – vasa silencing inhibits germ cell development pptx
... specifically expressed in germ cells of both male and female oysters, and we hypothesized that Oyvlg had a role in germ cell formation [17] However, the function of Oyvlg in germline development had ... reproductive deficiency The premeiotic male germ cells ceased their differentiation before the pachytene In vivo injection of oyvl-dsRNA provokes abnormal germ cell development One month postinjection, ... defects in germ cell development affecting all of the gonadic area, in both females and males Upon histological examination of gonads injected with 20 lg of dsRNA, there were fewer germ cells, and...
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Báo cáo khoa học: Wheat germ cell-free platform for eukaryotic protein production potx
... vitro cell- free methods for protein synthesis with extracts from prokaryotic [1] or eukaryotic [2] cells Wheat germ cell- free eukaryotic protein production offer an alternative to the E coli cell- based ... wheat germ cell- free method comparable to that of the E coli cell- based approach for NMR structure determinations of eukaryotic proteins One of the main advantages of the automated wheat germ cell- free ... normal multinuclear NMR spectra Cell- free protein production protocols are streamlined compared to cell- based protocols, in that they not require cell harvesting or cell lysis Protein purification...
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Báo cáo khoa học: Pathways involved in testicular germ cell apoptosis induced by H2O2 in vitro doc
... testicular Leydig cells in vitro [11] 874 Germ cells constitute the major inner seminiferous tubular cell mass and the role of H2O2 as a signalling molecule in apoptotic induction among germ cells is ... penicillin The cell viability of the final crude population that contained mostly maturing germ cells was in excess of 95% H2O2 treatment The isolated testicular germ cells (5 · 106 cellsÆmL)1 per ... Involvement of Bcl-2 family proteins in germ cell apoptosis during testicular development in the rat and pro-survival effect of stem cell factor on germ cells in vitro Mol Cell Endocrinol 165, 115–129 25...
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Germ Cell Tumor Edited by Angabin Matin pptx
... Testicular Germ Cell Tumors 75 Delphine Carouge and Joseph H Nadeau Chapter Epigenetic Modifications in Testicular Germ Cell Tumors 107 Christopher J Payne Chapter Claudins and Germ Cell Tumors Ylermi ... options for the most common type of germ cell tumors of the testes, non-seminomatous germ cell tumors In Chapter 3, Takamitsu Fujimaki reviews intracranial germ cell tumors, which affect mostly children, ... presentation, current treatment modalities and the biology and genetics of germ cell tumors Most authors have focused on testicular germ cell tumors which are the most common cancers in young adult males...
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Báo cáo khoa học: The consensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system ppt
... protein N-myristoylation in plant cells in more detail, we established a cell- free protein N-myristoylation system as an application of an advanced wheat germ cell- free translation system that ... Myr–GFP synthesized in the wheat germ cell- free translation system Effect of the combination of amino acids at positions and on protein N-myristoylation in the wheat germ cell- free translation system ... and of the consensus motif in plant target proteins with the use of a Cell- free N-myristoylation of plant proteins wheat germ cell- free translation system We identified several new N-myristoylated...
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germ cell protocols, volume 1
... Features Assay type Cell types Cells enter or cross cellulose membrane Cells move toward and enter capillary filled with attractant Cells avoid capillary filled with control buffer Cells migrate in ... and direction changes of individual cells (together referred to as “tracking” assays) as well as determinations of cell orientation, cell cell interactions, cell morphology, flagellar wave patterns, ... reservoirs; cells adhere to cover glass during migration Cells migrate between cover glass and slide; cells and chemoattractant released from wells Cells migrate across a circular bridge separating cell...
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germ cell protocols, volume 2
... midgestation of putative germ cell- derived clones, we hypothesize that either gonadal cells other than germ cells may have been picked instead of true germ cells or that a subset of germ cells may not have ... developmental potential of germ cell nuclei after transfer into oocytes remains controversial To date, gonadal cells bearing morphological resemblance to germ cells have been used or germ cells have been ... donor cells, either ES, EC, or germ cells could be used In this study, germ cells are used as they match cumulus cells for direct recovery from the body and absence of in vitro culture Gonadal cells,...
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báo cáo hóa học:" Role of the VEGF-Flt-1-FAK pathway in the pathogenesis of osteoclastic bone destruction of giant cell tumors of bone" pptx
... receptors in giant cell tumors (GCTs) of bone Surgical specimens were fixed in formalin and serially sectioned (a) VEGF was expressed in stromal cells (arrows), monocyte-like cells (arrowheads) ... VEGF was clearly expressed in stromal cells, monocyte-like cells and MNCs in GCTs CD68, an intracellular glycoprotein, was expressed in monocyte lineage cells, including OPCs and OCs [20] Therefore, ... of FAK in cells within GCTs Correlation between the clinical stage and pY-Flt-1 and pY-FAK expression in GCTs To determine the biological significance of VEGF-Flt-1FAK signaling in GCTs, we examined...
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báo cáo hóa học:" Role of the VEGF-Flt-1-FAK pathway in the pathogenesis of osteoclastic bone destruction of giant cell tumors of bone" potx
... receptors in giant cell tumors (GCTs) of bone Surgical specimens were fixed in formalin and serially sectioned (a) VEGF was expressed in stromal cells (arrows), monocyte-like cells (arrowheads) ... VEGF was clearly expressed in stromal cells, monocyte-like cells and MNCs in GCTs CD68, an intracellular glycoprotein, was expressed in monocyte lineage cells, including OPCs and OCs [20] Therefore, ... of FAK in cells within GCTs Correlation between the clinical stage and pY-Flt-1 and pY-FAK expression in GCTs To determine the biological significance of VEGF-Flt-1FAK signaling in GCTs, we examined...
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Báo cáo khoa học: "Comparative immunohistochemical characterization of canine seminomas and Sertoli cell tumors" ppt
... Leydig cell Sertoli cell Spermatogenic cell 1+ 2+ 1+ 1+ 2+ 1+ * = no staining, 1+ = ＜10% positive cells, 2+ = 10 to 50% positive cells, 3+ = ＞50% positive cells ... and Sertoli cell tumors filled the lumens or sheets of the affected seminiferous tubules These cells replaced the normal lining of spermatogenic and Sertoli cells Large and irregular cellular aggregates ... and canine Canine seminomas and Sertoli cell tumors testicular tumors In the current seminoma series, inhibin-alpha was observed in the cytoplasm of tumors cell in 45% of the cases Vimentin and...
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Báo cáo khoa học: "Mixed germ cell tumor metastatic to the skin: Case report and literature review" pptx
... 4.0% for renal cell carcinoma, 0.84% to 3.6% for transitional cell carcinoma, 0.4% for germ cell tumors, and 0.36% to 0.7% for prostate adenocarcinoma[9] Testicular mixed germ cell tumors are common, ... common, comprising roughly 33% of all testicular tumors The reason for such a high incidence of testicular mixed germ- cell tumors is because germ cells in the testes are totipotent and can undergo ... testicular tumors reported in the annual of pathological autopsy cases in Japan Hinyokika Kiyo 1983, 29:155-68 12 Bredael JJ, Vugrin D, Whitmore WF Jr: Autopsy findings in 154 patients with germ cell tumors...
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Báo cáo khoa học: " SemiIntensity modulated radiotherapy (IMRT) in benign giant cell tumors – a single institution case series and a short review of the literatur" docx
... giant cell tumors occurring outside the extremities in combination with a short review of the literature Patients and Methods Between 2000 and 2006 a total of five patients with giant cell tumors ... All tumors were histologically proven before start of the treatment All patients except one with a giant cell tumor in the sphenoid sinus suffered from large tumors in the sacral region Three tumors ... 25 (8%) patients after surgery The appearance of malignant giant cell tumors of bone or malignant foci inside benign giant cell tumors has been described also in a small number of patients [24,25],...
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báo cáo khoa học: "SMI of Bcl-2 TW-37 is active across a spectrum of B-cell tumors irrespective of their proliferative and differentiation status" pptx
... established human B -cell tumor lines and fresh patient samples representing the spectrum of B -cell tumors in man Our results demonstrate activity of TW-37 across all B -cell tumors irrespective ... complexity in B -cell tumors Our results presented here, show that small-molecule inhibitors of the Bcl-2 family proteins has a therapeutic role in a wide spectrum of B -cell tumors All four cell lines ... cytotoxic effect on primary lymphoma cells The CLL/SLL cells expressed CD5, CD19, CD20 and faint monotypic SIg The MZL cells were CD5-, CD19+ and CD20+ Mononuclear cells were separated by Ficoll-Hypaque...
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báo cáo khoa học: " Urethral metastasis from non-seminomatous germ cell tumor: a case report" pptx
... (Figure 1c) The diagnosis was of an International Germ Cell Cancer Collaborative Group (IGCCCG) poor prognosis metastatic nonseminomatous germ cell tumor Four cycles of bleomycin, etoposide and ... and is mainly a result of local spread from the surrounding organs Germ cell tumors of the testis are the most common solid tumors in men aged between 20 and 35 years We are not aware of any ... European Consensus Conference on Diagnosis and Treatment of Germ Cell Cancer: A report of the Second Meeting of the European Germ Cell cancer Consensus group (EGCCCG): Part Eur Urol 2008, 53:478-496...
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Báo cáo y học: " Extragonadal germ cell tumor presenting in a woman with systemic lupus erythematosus: a case report" ppt
... Matsuda H: Primary germ cell tumors in the mediastinum: a 50-year experience at a single Japanese institution Cancer 2003, 97(2):367-376 Droz JP, Horwich A: Extragonadal germ cell tumors Comprehensive ... patient’s pituitary showing polymorphic cells with granular cytoplasm Discussion This case study is unusual because our patient had both SLE and pituitary germ cell tumor It is also unusual because ... Rheum 2003, 48:2100 doi:10.1186/1752-1947-4-97 Cite this article as: Shahrir et al.: Extragonadal germ cell tumor presenting in a woman with systemic lupus erythematosus: a case report Journal of...
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Báo cáo y học: "Tenosynovial giant cell tumors as accidental findings after episodes of distortion of the ankle: two case reports" ppsx
... composed of round to polygonal cells Some were spindle cells and some were multinucleated giant cells (Figure 2B) The diagnosis of localized tenosynovial giant cell tumor of the tendon sheath ... the growth of fibroblastic cells is followed by a reactive proliferation of histiocytic cells in the reticuloendothelial system After phagocytosis of erythrocytes the cells undergo a transformation ... right the showSagittal T1-weighted giant cell tumor anterior toankleankle Sagittal T1-weighted spin-echo MRI of the right ankle showing a well delineated giant cell tumor anterior to the ankle (arrow)...
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Báo cáo y học: "Unusual clear cell tumors of the jaws – clinical and histopathological considerations: A case report" docx
... Altini M, Favia G: Clear cell tumors salivary glands, jaws and oral mucosa Semin Diagn Pathol 1997, 14(3):203-212 Jones GM, Telfer MR, Evenson JW: Metastatic renal clear cell carcinoma of the jaws ... were Discussion and conclusion Clear cell neoplasms of the jaw are very rare In a review of the literature, only isolated cases of metastatic renal clear cell carcinoma of the jaw have been described ... support in the final histological analysis References In the maxillo-facial area, clear cell odontogenic tumors have also been documented that are potentially aggressive and capable of multiple...
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Cell Cycle Regulation in Mammalian Germ Cells
... protein that promotes cell survival and has a role in maintenance of reproductive germ cells The knockout leads to progressiveloss of germ cells, Sertoli cells and Leydig cells and cause extensive ... deriving germ cells from human ES cells will permit studies of germ cell differentiation (Surani 2004) Not only will these approaches be used to identify the signals involved in regulating germ cell ... mediating cell cycle regulators, growth factors and intracellular communication pathways for germ cell development Cytoplasmic and Apoptotic Factors During gametogenesis, apoptosis of germ cells...
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Báo cáo khoa học: Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ pdf
... purified by gel filtration with NICK columns and finally by ethanol precipitation Cell- free translation The wheat -germ extract was prepared by the reported method [1] DTT in the extract (4 mM) was ... the wheat -germ extract at a high concentration for the sake of stability during storage [1] Thus, in order to reduce the DTT concentration for the translation reaction, Ó FEBS 2003 Cell- free ... the crude cell- free protein synthesis solution The present biotinylation technique may be useful for preparation of biotin-labeled proteins and also for nonradioisotope detection of cell- free...
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báo cáo khoa học: "Odontogenic tumors and giant cell lesions of jaws - a nine year study" docx
... odontogenic tumors( 8.5%), calcifying epithelial odontogenic tumors( 7.1%), odontomas (4.5%), odontogenic myxomas(4.5%) and odontogenic fibromas(2.8%) Among the giant cell lesions, central giant cell ... The tumors next in frequency to ameloblastomas, and second in the series of odontogenic tumors were adenomatoid odontogenic tumors (Figure 5), constituting 8.5% (N = 6) of the 70 odontogenic tumors ... giant cell granuloma showing vascular fibroblastic stroma and multinucleated giant cells H&E 10× Page of from other tumors developing in the oral cavity and facial bones [3] While the giant cell...
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