genes and genetics of murine lupus

Báo cáo y học: "The P2X7 receptor is a candidate product of murine and human lupus susceptibility loci: a hypothesis and comparison of murine allelic products" potx

Ngày tải lên : 09/08/2014, 06:22

... flow cytometric and IL-1β secretion experiments, and wrote the first draft of the manuscript JHM designed allele-specific primers and typed the P2RX7 genes of NZB mice and NZW mice, and 16 17 Tsao ... P2X7-stimulated shedding of CD62L by lymphocytes To enable the direct comparison of responses of cells from New Zealand Black (NZB) mice, lymphocytes New Zealand White (NZW) mice and (NZB × NZW)F1 (NZB/W) ... suggest that the P2X7 receptor and gene have the functional and positional characteristics suggestive of a role in the pathogenesis in SLE, and that the potential of the cell death mechanism aponecrosis...

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Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt

Ngày tải lên : 30/03/2014, 13:20

... pathway of GDP-L-fucose in mouse (Eur J Biochem 271) 79 Fig Gene structures of the short and the long splice variants of mouse fucokinase (A) and human fucokinase (B) The long splice variant of mouse ... L-fucokinase and pyrophosphorylase The primer and probe sequences and their positions in the mRNA sequence, for GDP-L-fucose pyrophosphorylase and the short and long splice variants of L-fucokinase, ... lane 2, short splice variant of mouse fucokinase and lane 3, long splice variant of mouse fucokinase Fig Fucokinase activities of the cell lysates of COS-7 cells (A) and HeLa cells (B) transfected...

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Báo cáo y học: "A prospective study of androgen levels, hormone-related genes and risk of rheumatoid arthritis" pptx

Ngày tải lên : 09/08/2014, 14:22

... our past findings of associations between reproductive factors and the risk of developing RA in the NHS [56] Data on pack-years of smoking (product of years of smoking and packs of cigarettes per ... the activated androgen–androgen receptor complex activates the expression of other genes via ligand binding, homodimerization, nuclear translocation, DNA binding, and formation of complexes with ... of data, and manuscript preparation PAF participated in the study design and interpretation of the data ST participated in the study design, statistical analysis and interpretation of data, and...

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Báo cáo khoa hoc:" Development and assignment of bovine-speciﬁc PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probes" ppt

Ngày tải lên : 09/08/2014, 18:21

... presence of the gene of interest was conrmed by PCR on the BAC DNA For the three BAC clones identied using PCR systems and BAC for the Texas marker genes 197 Figure Metaphase spread of bovine R-banded ... without pseudogenes Thus they may not be 198 M Gautier et al Figure Ideogram of bovine R-banded chromosome 29 indicating the position of the LDHA gene the most appropriate marker genes for standardisation ... ambiguities of the bovine karyotype nomenclature, particularly concerning BTA25, BTA27 and BTA29, and could be used as a standard for cytogeneticists using different banding techniques (G, R and Q)...

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Báo cáo y học: "Genome-wide prioritization of disease genes and identification of disease-disease associations from an integrated human functional linkage network" pps

Ngày tải lên : 09/08/2014, 20:20

... is the number of non-seed genes above the cutoff, True negatives is the number of non-seed genes below the cutoff, and False negatives is the number of seed genes below the cutoff Random prediction ... the number of seed genes above the Si cutoff, FP (false positive) is the number of non-seed genes above the cutoff, TN (true negative) is the number of non-seed genes below the cutoff, and FN (false ... below the cutoff, FP is the number of genes associated with neither disease above the cutoff, and FN is the number of disease II genes below the cutoff Finally, we calculate the AUC of the ROC...

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Báo cáo y học: "Development and management of systemic lupus erythematosus in an HIV-infected man with hepatitis C and B co-infection following interferon therapy: a case report" doc

Ngày tải lên : 11/08/2014, 17:21

... between HCV, HIV and SLE and antiviral immunity mediated by IFNa and pDC INF therapy is a critical component of current HCV treatment and its risk including development of autoantibodies, and autoimmune ... Loss of pDC in HIV infection usually parallels the loss of CD4 [15], but the rate of recovery of DC and CD4 T cells may be independent What factors may have been important in the development of ... Changes in viral load and lymphocytes pre and post therapy A: summary of therapy B: lymphocyte subset numbers and C: lymphocyte proportion during ART and in response to splenectomy and anti-retroviral...

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Báo cáo khoa học: " Enhanced detection and study of murine norovirus-1 using a more efficient microglial cell line" pps

Ngày tải lên : 12/08/2014, 04:20

... the appearance of viral-induced CPE (rounding of cells, loss of contact inhibition, and cell death) and the production of infectious viral particles The results show that all three murine- derived ... and Human Health (COHH) program, of the National Institutes of Environmental Health Sciences (P50ES012740), National Institutes of Health, and the National Science Foundation (OCE04-32479), and ... the design of the study by providing technical assistance to CC regarding plaque assays YL conceived of the study, and participated in its design, coordination, and data analysis and manuscript...

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Báo cáo y học: "Eukaryotic large nucleo-cytoplasmic DNA viruses: Clusters of orthologous genes and reconstruction of viral genome evolution" pps

Ngày tải lên : 12/08/2014, 04:21

... potential of comparative and evolutionary genomics of the NCLDV increase A pre-requisite of an informative comparative-genomic study of any group of organisms is an accurate delineation of the sets of ... consisting of genes with different degrees of evolutionary conservation, are in need of and amenable to the same approach Here we describe the construction of clusters of orthologous genes for ... of genes of cellular organisms, in all likelihood, were acquired in the course of evolution of the mimivirus lineage, probably, from a variety of distinct cellular sources; the same process of...

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Báo cáo y học: "4th meeting of the EU research network EUROME: From the identification of genes and cellular networks in murine models of arthritis to novel therapeutic intervention strategies in rheumatoid arthritis, London, UK, 9 March 2004" pot

Ngày tải lên : 09/08/2014, 01:23

... Ibrahim and Paleolog as lymph nodes and joints at various stages of disease in susceptible DBA/1J and resistant FVB/N strains In parallel, a genome screen was performed of the F2 progeny mice of a ... activation, as well as expression of antiapoptotic proteins Bcl-2 and members of the inhibitor of apoptosis family (cIAP-1, XIAP and survivin, which directly bind to and inhibit caspases), was strikingly ... either T helper (Th) or Th2 effector cells The goals of this arm of EUROME are to identify genes and pathways involved in this differentiation of DCs and to set up an ex vivo–in vivo cell therapy whereby...

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Báo cáo y học: "Abnormal costimulatory phenotype and function of dendritic cells before and after the onset of severe murine lupus" pot

Ngày tải lên : 09/08/2014, 07:20

... three murine models of lupus [8,9,11] and therefore that it is likely to be important in lupus development DCs from lupus mice express an altered ratio of CD80 to CD86 ex vivo The analysis of the ... because of impaired activation of regulatory T cells CD40-CD40L interaction has been found to be involved in the pathogenesis of lupus in four murine models [33-37], and ectopic expression of CD40L ... onset of the disease resembles the defective costimulatory profile found in DCs from patients with SLE [2,4] and validates the use of NZM2410 and NZB-W/F1 strains as murine models for the study of...

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Báo cáo y học: "The in vivo expression of actin/salt-resistant hyperactive DNase I inhibits the development of anti-ssDNA and anti-histone autoantibodies in a murine model of systemic lupus erythematosus" ppt

Ngày tải lên : 09/08/2014, 07:20

... SLE have been shown to cluster in and on the surface blebs of apoptotic cells [7,8] and ablation in mice of a number of genes whose products mediate the clearance of apoptotic cells, such as C1q ... salt-resistant and hyperactive DNase I has potential to alter the development of autoimmunity Materials and methods Construction of DNase transgenic mice The cDNA encoding murine wt.DNase I and a murine ... characterised by the development of a range of autoantibodies of both the IgM and IgG isotypes, and subsequent glomerular disease [37,38,49,50] In our hands, high levels of IgM anti-ssDNA antibodies...

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Báo cáo y học: "Underexpression of mitochondrial-DNA encoded ATP synthesis-related genes and DNA repair genes in systemic lupus erythematosus" pot

Ngày tải lên : 12/08/2014, 15:23

... Efficiency of the DNA repair and polymorphisms of the XRCC1, XRCC3 and XRCC4 DNA repair genes in systemic lupus erythematosus Lupus 2008, 17:988-995 Fernandez D, Perl A: Metabolic control of T cell ... micrograms of aRNA Page of from each sample (21 SLE patients and 45 healthy individuals) and the equivalent quantity of reference aRNA from a mixture of RNA extracted from peripheral blood of 45 healthy ... intensities of noprobe spots were used as the background The median and standard deviation of background levels were calculated Genes whose intensities were less than the median plus two standard...

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Tài liệu GENETICS IN PREVENTION AND TREATMENT OF CANCER ppt

Ngày tải lên : 15/02/2014, 04:20

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Tài liệu CANCER, GENES AND INHERITED PREDISPOSITION OvERvIEw – Cancer Genetics pptx

Ngày tải lên : 15/02/2014, 04:20

... they can be thought of as ‘cancer protection’ genes We all have two copies of a number of different ‘cancer protection’ genes in our cells, one copy inherited from our mother and one copy inherited ... protection’ genes have in the development of cancer? The cycle of cell division and growth continues throughout the lifespan Changes (mutations) in the ‘cancer protection’ genes that make them faulty and ... many ‘cancer protection’ genes in the cells www .genetics. edu.au CANCER, GENES AND INHERITED PREDISPOSITION Overview – Cancer Genetics a) There are ‘cancer protection’ genes on different chromosomes...

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Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc

Ngày tải lên : 20/02/2014, 11:20

... enhancement of adenovirus infection at each of the concentrations tested and a positive relationship between dose and number of GFP-expressing cells for both immature and mature murine Ó FEBS ... detectable homology to CD40 ligand (data not shown) and that enhances adenoviral infection of CD40-positive cells of both human and murine origin, including DC and B cells resistant to infection ... ﬂuorescence of uninfected cells, and the percentage of GFP positive cells is shown Error bars represent the standard deviation of triplicate infections Results shown are representative of three...

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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Ngày tải lên : 07/03/2014, 12:20

... gene promoters and to the LCR of the murine b-globin gene locus in MEL cells (A) Schematic representation of the murine b-globin gene locus LCR hypersensitive sites and globin genes are shown ... indicating that dimethylation of H3K4 and recruitment of RNA Pol II and TBP to the LCR occurs before activation of any of the globin genes The presence of H3 dimethylated at K4 indicates that ... the temporal nature and extent of covalent histone modiﬁcations and association of transcription complexes at the murine b-globin locus during the in vitro differentiation of murine embryonic stem...

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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

Ngày tải lên : 07/03/2014, 15:20

... product of ml1p variants giving 169 and 246 bp fragments SalI cuts the ampliﬁcation product of ml2p, giving 66, 121 and 243 bp fragments, and the amlpiﬁcation product of ml3p and ml3.1p, giving 50 and ... The product of the second round of PCR was cloned and the 1066 bp sequence encoding 125 amino acids of the A-chain, the 19 amino acids linker, and 216 amino acids of the B-chain of the ml gene ... separated on 2% agarose gel and the bands corresponding to genomic fragment (length of 415/430 bp) and competitor fragment (length of 388 bp) were excised Radioactivity of the bands was then measured...

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Báo cáo Y học: Cloning, chromosomal localization and characterization of the murine mucin gene orthologous to human MUC4 pdf

Ngày tải lên : 08/03/2014, 23:20

... transcript of a predicted size of 13 kb Both human MUC4 and mouse Muc4 genes are virtually identical in terms of the class of introns, the exon number and size of exons The sizes of the 24 mouse ... mapped; (c) Muc4 and MUC4 have a similar pattern of expression; (d) the tandem repeat sequences of Muc4 share homology with the tandem repeat sequences of SMC; and (e) BLAST searches and multiple ... last exon is composed of a coding sequence of 193 bp and of an untranslated region of at least 131 bp The size of the 24 introns ranges from 79 bp to about 16 kb The size of the largest intron...

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Báo cáo khoa học: Distribution of the lipolysis stimulated receptor in adult and embryonic murine tissues and lethality of LSR–/– pdf

Ngày tải lên : 16/03/2014, 18:20

... (430–53 1of 4467) 124 (1926–2049 of 5521) 131 (520–650 of 1785) 65 (771–835 of 2354) 79 (134–212 of 936 CDS) 126 (268–393 of 924) 72 (1084–1155 of 2031) 75 (1010–1084 of 1172) 190 200 Zealand ... comprised of left and right homology arms, which consisted of 2.65 kb of cloned genomic DNA sequence containing the 5¢part of exon 2, intron 1, exon and its 5¢-ﬂanking noncoding sequence, and 2.7 kb of ... a null allele by deletion of an internal region of the gene between the 5¢-end of exon and the 3¢-end of exon and its substitution with a reporter (b-galactosidase) and selection marker (neomycin...

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Neural Tube Defects – Role of Folate, Prevention Strategies and Genetics Edited by Kannan Laksmi Narasimhan ppt

Ngày tải lên : 17/03/2014, 02:20

... prevention of infection, covering of the myelomeningocele, control of hydrocephalus, management of urinary dysfunction, and treatment of the paralysis and abnormalities of the hips and feet (Gleeson ... results in terms of knowledge about the benefits and sources of folic acid, and especially in terms of understanding the correct, periconceptional timing of folic acid intake Official health education ... (i.e., the proband) and his or her (their) parents, sibs, and maternal grandparents Doolin et al (2002) assessed associations between maternal and offspring MTR and MTRR genotypes and spina bifida...

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