genes affecting starch and protein composition of the kernel
Starch and Protein Quality Requirements of Japanese Alkaline Noodles (Ramen).pdf
... score of 14 points, or 70% of the maximum 20 points allocated for texture, at each of the two times of assessment Total texture score was the sum of the two scores The median score of the four ... lowest (15.1 mL/g) of the values for the sample set The PV and BD of the commercial sample assessed in mM AgNO3 were the second lowest and third lowest of the sample set (238 and 125 RVU, respectively) ... from these analyses and the texture of ramen prepared from the same flours were examined Other analyses included FN tests on the 20 wheat samples, and protein and ash determinations on the flours...
- 7
- 978
- 4
Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The ... Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose are involved in ... Asp142, 2¢ and 3¢OH of the ribose and three water molecules with ˚ coordination distances of approximately 2.4 A The phosphate of the GMP is hydrogen bonded to main chain and side chain atoms of amino...
- 11
- 770
- 0
Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf
... in 87% of the cases; and residue 334 a His in 80% of the cases [3] In addition, residue 54 is a Pro in 89% of the cases The importance of the identity of the residues present in these and adjacent ... transition of PAI-1 wild-type, and the use of this pH therefore allowed optimization of the difference in the effect of vitronectin on wild-type and the unstable variants The presence of vitronectin ... by affecting the rate of passage of the RCL through the gate region Based on the aminoacid sequence of the RCL and b strand 5A being directly continuous, it may be proposed that movements of the...
- 10
- 431
- 0
Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... the presence of these amino acids and amino acid derivatives, and values of DGD°, measured in triplicate, are given in Table The effect of polyols on the kinetic parameters (Km and kcat) of the ... preferential hydration of the protein domain is the driving force of protein stabilization or folding [3,4] Furthermore, the effect of osmolytes on the functional activity of an enzyme has also ... reaction of the free enzyme and free substrate [29] We have therefore estimated kcat ⁄ Km values of all the reactions in the presence and absence of all classes of osmolytes It can be Fig Effect of...
- 9
- 547
- 0
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... contain up to 230 proteins and about 65 of these are IgE binding proteins [20]; however, some of these are known to be either isoforms or the degraded products of allergens Furthermore, allergic ... sequence and sequence alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide sequence The ... in the pollen As a consequence, individuals allergic to BGP require individual diagnosis and therapy, and an understanding of the structure of these allergens is essential to the improvement of...
- 10
- 665
- 0
Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
... localization and hormonal regulation of the PKI and PKI isoforms of the inhibitor protein of the cAMP-dependent protein kinase J Biol Chem 272, 20011–20020 Byler, D.M & Susi, H (1986) Examination of the ... studies of the catalytic subunit of the cAMP-dependent protein kinase Its ability to readily alter the PKI coding sequence could permit further studying of human PKIb and understanding of the function ... indicated by the presence of a very strong negative band at 198 nm The negative band near 220 nm results from overlapping of the bands of b-sheet (215 nm) and a-helix Table Fractional composition of secondary...
- 6
- 531
- 0
Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the structures of these ... [33] The errors of R1 and R2 were estimated from the errors in the single exponential decay fitting, and those of the 15N-1H NOEs were obtained from the difference of two independent experiments The ... solubilized the protein fragment in 10 mm CHAPS buffer The combination of these two A Dynamic structure of XPC-binding domain of hHR23B procedures markedly improved the quality of the 15NHSQC...
- 10
- 431
- 0
Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... topology where the loop is in the thylakoid lumen, and the N -and C-termini face the stroma, opposite to that of PSI-K On the other hand, the second scenario implies a stromal loop, like that of PSI-K, ... of their crystal structure of Pisum sativum PSI, in which the loop of PSI-G is suggested to face the stroma The amount of PSI-G in the transformed knock-out line does not reach the level of the ... integration of the subunit into the PSI complex A protein band smaller that the tagged versions of PSI-G is present in the lanes with the loop-tagged PSI, but is also seen faintly in the lanes with the...
- 9
- 422
- 0
Báo cáo khoa học: Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identified by site-directed mutagenesis pot
... Results PCR mutagenesis of CL1 and CL2 site residues of tACE and ACE2 The residues surrounding the chloride ion at the CL1 and CL2 sites of tACE and ACE2 are shown in Fig In tACE, the residues that ... obtained for the inhibition of the ACE2 variants by MLN-4760, and the inhibition of the tACE variants by captopril, in the absence and presence of NaCl (500 mm) The IC50 values derived from these dose–response ... [23] The CL2 chloride site is absent in ACE2 as a result of substitution of the tACE residues Pro407 and Pro519 with Glu398 and Ser511, and the resulting projection of their side-chains into the...
- 10
- 362
- 1
Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx
... minor forms of the holoACP structure, on the basis of their percentage contributions (65% and 35%, respectively) to the overall structure of the protein The structures of the major and minor conformations ... regulated The differences in stability between the two proteins (as indicated by the values of DGwater, Cm and Tg) hence arise from the changes in the surface of the protein because of the interactions ... slightly; there is a difference of almost C between the Tg values of the proteins, the value being higher for the holo form There have been contrasting reports about the interaction of the 4Â-PP...
- 14
- 419
- 0
Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx
... of the medium and K2 the orientation factor, determined by the mutual spatial orientation of the transition dipole moments of the donor and acceptor As no data on the spatial orientation of the ... quenching of protein Trp fluorescence and in an increase of the specific DAUDA emission after the noncovalent incorporation of the ligand Binding of DAUDA to Ag-NPA-1 caused 20% quenching of the emission ... in cells of the inner hypodermis and the epithelium of the oviduct as well as extracellularly in the pseudocoelomic cavity of the worms Discussion A bundle of four a-helices constitutes the secondary...
- 10
- 501
- 0
Báo cáo Y học: Synthesis and turn-over of the replicative Cdc6 protein during the HeLa cell cycle potx
... examined, and that about one half of the newly synthesized hCdc6p associated with chromatin during the 2-h label period Rates of hCdc6p synthesis To investigate whether the synthesis of hCdc6p ... [35S]methionine for h at the beginning (0–2 h after thymidine-block) and in the middle of S phase (4–6 h), as well as at the end of mitosis and during the early G1 phase (12–14 h) of the next cycle (Fig ... preferentially attacks the phosphorylated form of hCdc6p However, more importantly in the present context, we note that the synthesis of hCdc6p continues through most of the cell cycle Synthesis of hCdc6p...
- 7
- 554
- 0
Báo cáo khoa học: Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida pptx
... to that of PMT-C3 is that, at the sequence level, there is no discernible similarity of PMT-C3 to the proteins, with the exception of the Cryseobacterium protein glutaminase However, there is ... cellular outcomes, the sequence and structure of the activity domain of PMT differ considerably from those of the other dermonecrotic toxins [3] and point to a clear functional example of convergent ... this review, we focus on PMT and our current understanding of the structure–function, mechanism of action and cellular consequences of this newest member of the G -protein- deamidating dermonecrotic...
- 17
- 402
- 0
Báo cáo khoa học: Refined solution structure and backbone dynamics of the archaeal MC1 protein ppt
... additional DNA and the body of the protein [19] In the case of MC1ÆDNA complexes, we know that the protein covers at least 15 bp and that the binding site is composed of two areas of contact separated ... inwards, the arms close and the tips of the arms wrap around the DNA The amplitudes and time scales of the intramolecular motions experienced by the protein backbone are commonly determined from the ... with the DNA-binding b-hairpin arms of HUs, which showed high mobility relative to the core (Fig 2) The b-ribbon arms wrap around the minor groove of the DNA and, at the tip of each arm, the conserved...
- 13
- 387
- 0
Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA docx
... stained with the DNA dye Hoechst; GFP, indicates the localization of the green fluorescent protein; RFP, indicates the localization of the red fluorescent protein GFP + RFP, merging of green and red ... accumulation of the pre-rRNA 35S and the aberrant 23S, and decreased levels of the 20S pre-rRNA and the mature 18S rRNA, although to different degrees of severity [10–12,14,25–28] These results ... depletion of Utp25p there is an accumulation of the pre-rRNA 35S and the aberrant 23S, and a decrease in pre-rRNA 20S and mature 18S rRNA (Fig 5A) The large ribosomal subunit RNAs 25S, 5.8S and 5S...
- 15
- 328
- 0
Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunflower KNOX protein HAKN1 with DNA ppt
... aggregation of the protein Nevertheless, different protein preparations showed the same specificity and affinity when considering the amount of bound protein as the sum of both shifted bands These bands ... The protein establishes contacts with both strands at the right side of the GAC core, while only one strand seems to be contacted at the left side This lack of symmetry and the extension of the ... towards the 3¢ end of the top strand and the 5¢ end of the bottom strand (Fig 4B) This agrees with the presence of a larger residue that makes stable contacts with this region of DNA The interaction...
- 13
- 558
- 0
Báo cáo khoa học: Increased sensitivity of glycogen synthesis to phosphorylase-a and impaired expression of the glycogen-targeting protein R6 in hepatocytes from insulin-resistant Zucker fa ⁄ fa rats pptx
... inactivation of glycogen synthase and inhibition of glycogen synthesis The rate of glycogen synthesis, but not the activity of glycogen synthase, inversely correlated with the activity of phosphorylase-a ... phosphorylase-a and glycogen synthesis Using three independent methods involving either expression of the muscle isoform of glycogen phosphorylase, or expression of the glycogen-targeting protein PTG ... property of the entire metabolic system and depends on the concentrations of other proteins that influence the flux through that pathway This study shows that the flux-control coefficient of phosphorylase...
- 11
- 360
- 0
Báo cáo khoa học: Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7 ) from zebrafish (Danio rerio) potx
... exons and coding for B-FABP This result is consistent with previous observations for the human and rat I-FABP, and other members of the FABP family, that the N-terminal halves of these proteins ... the FABP genes and other members of this multigene family reported thus far [24], with the exception of the desert locust muscle-type FABP which lacks intron [25] The coding sequence of the zebrafish ... amplifications to determine the standard curve of the crossing points for the amplification of B-FABP and b-actin from tissue-specific cDNA samples Melting curve analysis of each standard and experimental...
- 11
- 366
- 0
Báo cáo sinh học: " Relationship between the loss of neutralizing antibody binding and fusion activity of the F protein of human respiratory syncytial virus" doc
... mechanism of action of HRSV neutralizing antibodies and the impact of resistance to these antibodies upon the fusion activity of the F protein A summary of these results is presented in Table The HRSV ... reagents and performed the fusion assays PB and ND performed the flow cytometry LG conducted sitedirected mutagenesis of the HRSV F protein AD and RS participated in the design, oversight of the conduct ... insights into the function of the F protein, and help in the selection and development of clinical candidates, such as ch101F, for next generation antibody-based prophylaxis and therapy for HRSV...
- 4
- 271
- 0
Báo cáo hóa học: " Relationship between the loss of neutralizing antibody binding and fusion activity of the F protein of human respiratory syncytial virus" doc
... mechanism of action of HRSV neutralizing antibodies and the impact of resistance to these antibodies upon the fusion activity of the F protein A summary of these results is presented in Table The HRSV ... reagents and performed the fusion assays PB and ND performed the flow cytometry LG conducted sitedirected mutagenesis of the HRSV F protein AD and RS participated in the design, oversight of the conduct ... insights into the function of the F protein, and help in the selection and development of clinical candidates, such as ch101F, for next generation antibody-based prophylaxis and therapy for HRSV...
- 4
- 252
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25