generation of germline derived stem cells from the adult human testis
Báo cáo y học: "Hypoxic conditions increase hypoxia-inducible transcription factor 2α and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients" pps
... Arthritis Research & Therapy Vol No Khan et al practical access, cells from the IPFP are of interest as a potential source of cells for the repair of focal cartilage defects in the knee [5] In previous ... reported in 2% oxygen [36] The effects of hypoxia on the propagation of human bone marrow -derived stem cells suggested that 2% oxygen favoured more primitive stem cells with higher colony-forming ... Chondrogenic culture of IPFP cells and the effect of low oxygen tension The cultured cell aggregates of IPFP cells with chondrogenic medium showed evidence of induction of chondrogenesis under...
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Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx
... of proteoglycans after pellet co-culture of disc cells with BM-MSC [20] Stimulation of disc cells with stem cells or CM could enhance the success of autologous implantation of disc cells In the ... & Therapy Vol 10 No Tapp et al Adipose -derived mesenchymal stem cells (AD-MSC) offer some advantages as an attractive, readily available adult stem cell because of the ease of harvest and their ... ratio of AD-MSC and annulus cells in coculture compared with the predicted value calculated from the sum of the proteoglycan contents of individual cultures of ADMSC and annulus cells Data from...
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Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells
... another group reported the importance of Klf5 in mediating the self-renewal of mouse ESCs.59,60 They reported that the ablation of Klf5 prevented the successful derivation of mouse ESCs from the ... characteristics In another instance, pluripotent cells can also be derived from germline stem cells harvested from neonate mouse testes43 as well as adult testes44 Interestingly, parthenogenesis of an unfertilised ... the early developing embryo These versatile cells, known as embryonic stem cells (ESCs), are derived from the epiblast of the early peri-implantation mouse embryo11 and are characterised by their...
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Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot
... markers on their surface such as CD34 and CD133 allowing for the isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While ... While previous attempts have focused on the cell culture system, in this study the phosphoproteome of the primary cells from the immediate collection of HSCs from patient blood or bone marrow was ... characterization of phosphorylation is critical to the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications...
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o cáo hóa học:" Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pptx
... markers on their surface such as CD34 and CD133 allowing for the isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While ... While previous attempts have focused on the cell culture system, in this study the phosphoproteome of the primary cells from the immediate collection of HSCs from patient blood or bone marrow was ... characterization of phosphorylation is critical to the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications...
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Báo cáo y học: "dentification of blood biomarkers of rheumatoid arthritis by transcript profiling of peripheral blood mononuclear cells from the rat collagen-induced arthritis model" doc
... destruction of the extracellular matrix, and eventual damage to the affected joints [5,6] Although there have been many studies of cells within the arthritic joint, the responses of the peripheral ... and members of the functional genomics group for helpful discussion, and the members of the Shou laboratory for critical reading of the manuscript We also acknowledge the support from the bioinformatics/IT ... Data from each probe set were fitted to the above model independently as is done in other studies [30,31] To control the false positive rate of testing the expression change of thousands of genes...
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Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2" potx
... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells ... stem cells [48] FGF2 is routinely used in the culture of bone marrow -derived mesenchymal stem cells, and we have determined a baseline for the use of FGF-2 in the culture of fat pad -derived cells ... characterisation of infrapatellar fat padderived cells The rate of proliferation of the IPFP -derived cells in monolayer culture was significantly increased by FGF-2 A comparison of their proliferation...
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báo cáo hóa học:" Serous papillary adenocarcinoma possibly related to the presence of primitive oocyte-like cells in the adult ovarian surface epithelium: a case report" docx
... possible The primitive oocyte-like cells in the ovarian surface epithelium of this patient might have persisted from the fetal period of life or developed from the putative stem cells in the ovarian ... search for the presence of putative stem cells The analysis confirmed the presence of round, SSEA-4 and SOX-2-positive primitive oocyte-like cells in the ovarian surface epithelium These cells resembled ... VASA-positive cells (arrows) in the ovarian surface epithelium and cortex (A, B) Smaller cells with diameters of to μm in the epithelium (C-F) Bigger cells with diameters of 10 μm in the epithelium...
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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... in human neuroepithelial-like stem cells for the generation of modified neuronal cultures 59 4.2 Generation of gene-corrected neural stem cells from MJD patient -derived iPS cells ... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... dividing stem cell state 1.1.1 Human pluripotent stem cells Landmark discoveries of the young field of human stem cell science were the isolation and culture of inner cell mass from human blastocysts...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... ES cells and show that these marks occur independently of the recruitment of any lineage-specific transcription factors such as PU.1 Furthermore, they observe the presence of components of the ... recruitment of factors to the a-globin gene locus during the differentiation of erythroid cells The regulatory elements located upstream of the a-globin genes also appear to initiate the activation of the ... HS3, the HS2 ⁄ HS3 flanking region, HS2, and the adult b-globin gene 20 and 30% of CD34+ cells, which are known to express low levels of the adult b-globin gene; this could explain the presence of...
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Stem CELLS and the FUTURE OF REGENERATIVE MEDICINE pdf
... with many human adult stem cells The application of stem cell research to therapies for human disease will require much more knowledge about the biological properties of all types of stem cells Although ... or the brain, in the adult body They can renew themselves in the body, making identical copies of themselves for the lifetime of the organism, or become specialized to yield the cell types of the ... into the various components of the blood and prevents them from developing into leukemic cells (Saito et al., 2000) OTHER ADULT STEM CELLS During the past years, scientific reports of stem cells...
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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...
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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx
... concentration in dogs anesthetized with isoflurane Am J Vet Res 2003, 64, 1155-1160 27 Ogawa R The importance of adipose -derived stem cells and vascularized tissue regeneration in the field of tissue transplantation ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose -derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... Results Differentiation test of ASCs Adipogenic differentiation of ASCs was apparent after weeks of incubation with adipogenic medium By the end of the third week, half of the cells contained Oil red...
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Báo cáo y học: "Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model" pps
... biology of the prostate: stem cell biology Urology 2003, 62:11-20 Bonkhoff H: Role of the basal cells in premalignant changes of the human prostate: a stem cell concept for the development of prostate ... and stem cell characteristics of the transformed P63+ basal /stem cells, supporting that they could be the origin of PCSCs under the influence of persistent Hedgehog overexpression P63+ basal /stem ... in the P63+ basal/ stem cells Patch1 and Smo were found highly expressed in the P63+ basal /stem cells of the pCX-shh-IG-injected prostates, being located in the cell membrane of P63+ basal cells...
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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx
... clarified before the general application of the procedure The mechanism of regeneration is not yet clear It could be through direct differentiation of stem cells that were introduced to the diseased ... presentations The following cases concern four different individuals Of the four, the first two cases involve bone regeneration in osteonecrosis of hips, the latter two cases regeneration of cartilage ... showed osteonecrosis of the femoral head, stage Since there is no effective non-surgical Page of treatment of the disease, she elected to receive stem cell treatment At the time of initial evaluation,...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... μl of PBS in the left ear DTH response was calculated as the percentage swelling (the difference between the increase of thickness of the right ear and the left ear, divided by the thickness of ... Results Generation of mesenchymal stem cells and phenotypical analysis The isolated mesenchymal stem cells differentiate into osteogenic and adipogenic lineages To assess the multipotentiality of the ... potential of MSCs in vitro, we tested their effect on the anti-CD3induced proliferation of CD4+ T cells T cells were stimulated in vitro with anti-CD3 antibody in the absence or presence of MSCs and their...
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Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps
... radiographic damage [31] The MMPs are key mediators of the invasive phenotype of FLSs [32] Therefore, we further investigated the effect of UC-MSCs on the invasive behavior of FLSs by the Cell Migration/Invasion ... and the downregulated Th1/Th17 cells Furthermore, in the UC-MSCs treated mice, the expansion of Th2/Tregs and the production of anti-inflammatory IL-10 were elevated MSCs have the capability of ... [44] Therefore, reduction of MCP-1 could partly explain the absence of inflammatory infiltrates in the synovium of mice treated with human UC-MSCs Moreover, UC-MSCs increased the levels of the...
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Báo cáo y học: "Vpx rescues HIV-1 transduction of dendritic cells from the antiviral state established by type 1 interferon" pps
... that the PCR products were a reflection of de novo cDNA synthesis in the target cells and were not the result of contaminating plasmid DNA carried over from the transfection used to generate the ... (Applied Biosystems), μL of undiluted cDNA, and μL of the specified TaqMan Gene Expression Assay (Applied Biosystems) on either the 7900HT Fast RealTime PCR System (Applied Biosystems) or the CFX96 ... explore the effect of Vpx on HIV-1 transduction of myeloid cells after an antiviral state had been established by administration of exogenous type IFN Page of 16 Human monocyte -derived dendritic cells...
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Báo cáo y học: "Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristic" doc
... Dendritic cells (DCs) are highly specialized antigen presenting cells that originate from bone marrow progenitor cells They represent a major cellular component of the innate immune system and their ... Table shows the corresponding protein data The factor of difference was calculated by dividing the intensity of the protein spot in mDCs by that of the corresponding spot in moDCs or KG-1 cells Eighteen ... then proceeded Despite the general similarities between mDCs and the two DC model systems, moDCs and KG-1 cells, our findings identified important differences between the proteomes of these cells, ...
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Effects of combined mechanical and pulsed electromagnetic field stimulations on the osteogenesis of bone marrow stem cells
... distribution of cells throughout the scaffold structure It is also important for the diffusion of nutrients and gases and the removal of metabolic waste from the cells growing within the scaffold The ... the use of porogens or any solvents Hence, there is no risk of undesirable residual substances left in the scaffold The physical nature of the process also does not change the properties of the ... single disulfide bond The disulfide linkage that holds the fibroin together is between the Cys-c20 (20th residue from the carboxyl terminus) of the heavy chain and Cys-172 of the light chain and...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25