generation and characterization of cardiomyocytes under serum free conditions pdf
Báo cáo khoa học: Identification and characterization of oxidized human serum albumin A slight structural change impairs its ligand-binding and antioxidant functions pptx
... L(small)-Trp as an endogenous ligand which binds to site II, and of cefazolin (site I-ligand) and verapamil (site I and II-ligand) as exogenous ligands All these ligands are known for their high ... fragment of HSA N, nonoxidized HSA; H, highly oxidized HSA (B) Densitometric analysis of intensities of undigested HSA Changes in intensities of HSA bands relative to the band of starting point of ... in place of l-cysteine, and homocystine was used in place of cystine LC-ESI-TOFMS was used to determine the purity of HSA-Cys and HSA-Hcy The additive reaction resulted in high yield and HSA(red)%...
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Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx
... regulations and guidelines of the National Institutes of Health (NIH) and with the approval of the TPPS Animal Care and Use Committee Mice were challenged with various concentrations of SEB (Toxin ... capable of neutralizing high doses of SEB both in vitro and in vivo Our lead antibody HuMAb154 was selected for further characterization because of its high affinity and in vitro high potency and ... concentrations of 50 nM, 25 nM, 12.5 nM, 6.25 nM, 3.13 nM, 1.56 nM and 0.78 nM The running buffer used was HBS-EP with a flow rate of 30 μl/min Regeneration of the surface was performed by two injections of...
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GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS
... biology and Conrad and Dominik for the initial generation of 10.15 To Dr Lok Shee-Mei, Petra and Jiaqi, thank you for solving the cryo-EM structure of 14C10 and 10.15 To our collaborators at NUH and ... Figure 23 Binding activity of 10.15 to various strains of DENV2 and DENV1, and 101 Figure 24 Binding activity of 12.17 to various strains of DENV2 and DENV1, and 102 ... Philippines and recent isolates from South Pacific islands Thailand, Vietnam and Bangladesh Sri Lanka, India, Africa, Samoa and 1962 strain from Thailand Puerto Rico, Latin and central America and 1965...
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Báo cáo khoa học: Metabolism of N-methyl-amide by cytochrome P450s Formation and characterization of highly stable carbinol-amide intermediate pdf
... and characterization of stable carbinol-amide L Perrin et al Table Amounts of metabolites M1 and M2 recovered after 10 incubations of 100 lM tentoxin using lM of rat microsomal preparations and ... 2174 and concentrations, followed by HPLC separation in the presence of acids or bases In the case of metabolism of TTX and its analogs, we have shown that under such conditions rapid cleavage of ... 20% over a period of days at C (Fig 1D) 2170 Fig Time-dependence evolution of puried M1 converting to M2 as a function of time and storage conditions (A) HPLC peak of puried M1 and its evolution...
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Báo cáo khoa học: Identification and characterization of multiple species of tenascin-X in human serum doc
... number of samples from serum stored over longer periods and fresh serum and found no evidence of the appearance of additional bands after storage (data not shown) Fig Amino acid sequence coverage of ... antiserum against FNIII29–30 of TNX and the signal for the bands corresponding to the 75 and 140– 150 kDa species are approximately of equal strength Most likely, the FNIII29–30 repeats of TNX ... tropoelastin and regulation of elastic fibre assembly or stability could reside in the 75 and 140–150 kDa C-ter- Multiple species of tenascin-X in human serum minal species The failure of serum TNX...
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Báo cáo Y học: Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin doc
... activities of the TRH-DEs in serum, liver and brain from rat, pig and cow Serum and tissue homogenates were prepared and analyzed as described in Materials and methods (n ¼ for pig and cow, n ... rate of TRH-degradation was determined by a four-point kinetic test Purification of the TRH-DE from porcine serum Preparation of tissue homogenates and serum After the animals were killed, blood and ... brain enzyme [28] After SDS/PAGE under reducing conditions a molecular mass of 125 000 Da was estimated for the liver enzyme and the serum enzyme and a molecular mass of 116 000 Da for the brain enzyme,...
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Báo cáo hóa học: "Synthesis and characterization of Nb2O5@C core-shell nanorods and Nb2O5 nanorods by reacting Nb(OEt)5 via RAPET (reaction under autogenic pressure at elevated temperatures) technique" potx
... sintering of the rods, which occurs upon annealing at 500 °C under air for h EDX measurements of Nb2O5@C core-shell nanorods and neat Nb2O5 nanorods indicates the presence of only Nb and oxygen and ... existence of 69.0 wt% of Nb and 31 wt% of O, which is very close to the theoretical value of Nb2O5 (Nb = 69.9 wt% and O = 30 wt%) The carbon peak originates from the carbon shell The peaks of copper ... excitonic or interband (valence conduction band) transitions of Nb2O5, which allows us to calculate the band gap Figure depicts the optical DRS of the Nb2O5 An estimate of the optical band gap is obtained...
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Modeling and characterization of abrasive free copper chemical mechanical planarization process
... the surface analysis and the results of MRR and WIWNU of blanket wafer polishing, fundamental understanding of abrasive -free CMP was developed and some optimized polishing conditions were selected ... [Moore, 1965] The high demand of integration and miniaturization has made the fabrication and design of microchip more complex The demand of fast shrinkage of feature size and high performance ICs ... abrasive -free CMP The fundamental understanding of this newly developed abrasive -free CMP process is essentially important for process optimization and control 2.4 Fundamental Study of Abrasive and...
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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge
... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the...
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Preparation and characterization of the PVDF-based composite membrane for direct methanol fuel cells
... diameter of the PVDF membrane, the PVDF-PS membrane and the PVDF-SPS membrane is 0.25 µm, 0.12 µm and 0.16 µm, respectively It can be seen that the polymerization of styrene and the blending of PS and ... International Journal of Energy and Environment (IJEE), Volume 1, Issue 4, 2010, pp.643-656 645 2.5 Measurement of proton conductivity The proton conductivity of the PVDF-SPS membrane and Nafion-117 ... compartment (mol.L-1) VB is the volume of right reservoir (cm3) A and L are the membrane cross-sectional area and thickness, respectively α is the slope of the linear plot of methanol concentration in...
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Design, fabrication, and characterization of a solenoidsystem to generate magnetic field for an ECR proton source
... system is capable of producing off-resonance, mirror, and flat magnetic field configurations A mirror ratio of 1·1 and a maximum flat field of 1400 G has been obtained The measured values and the design ... varying the size of the solenoid coils, thickness of iron jacket, and amp-turns (NI) The optimum values of NI for the side and middle solenoid coils were obtained to be 12950 and 8250 respectively ... μS/cm) having inlet temperature of 27◦ C Based on the length of the conductor, water pressure drop and flow, the inlet and outlet connections were provided Total five inlet and five outlet connections...
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Tài liệu Báo cáo "Preparation and characterization of nanocomposite TiO2/SnO2 films " pdf
... resistance (RD) and the light resistance (RL) under the irradiation of 7W Hg lamp at the distance of 10cm were measured The calculated ratio of RD/RL was considered as a photoconductivity of obtained ... spectra of TiO2 prepared at 400oC impregnated and annealed at 325, 375 and 425oC Discussion TiO2 belongs to dielectric materials With a wide band gap of 3.2 eV, at room temperature there are no free ... with the help of an electronic digital controller Under an open-air environment and at high temperature, hydrolysis of Ti salt solutions takes place, resulting in the formation of TiO2 deposited...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... tissue of mouse and spleen and thymus of human and mouse [11,37] Foxp3 is also highly expressed in lymphoid organs, such as the thymus and spleen [49], and its expression within a variety of other ... expression of t-bet, stat6 and foxp3 was observed in the spleen, kidney, gill, gut, liver and skin tissue of healthy fish (data not shown) After stimulation of kidney cells with a variety of immunostimulants, ... t-bet, stat6 and foxp3 were also determined and found to be very similar to their human homologues Both the human and zebrafish T-bet and Foxp3 genes contained the same number of exons and introns...
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Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs pdf
... Identification of the initiation codons in GmPDIL-3a and Gm-PDIL-3b mRNAs (A) Schematic representation of the structure of GmPDIL-3a and GmPDIL-3b mRNAs The putative ORFs (gray boxes) of GmPDIL-3a and GmPDIL-3b ... S1 Comparison of intron–exon structures of GmPDIL-3a, GmPDIL-3b and orthologs of other plants Fig S2 Multiple amino acid sequence alignment of GmPDIL-3a, GmPDIL-3b and orthologs of other plants ... and CD analysis of recombinant GmPDIL-3a and GmPDIL-3b Table S1 List of primers for ATG-mutagenesis of GmPDIL-3a and GmPDIL-3b This supplementary material can be found in the online version of...
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Tài liệu Republican Legal Theory The History, Constitution and Purposes of Law in a Free State ppt
... Self-Determination of Peoples (editor) Republican Legal Theory The History, Constitution and Purposes of Law in a Free State M.N.S Sellers Regents Professor of the University System of Maryland and Director of ... author of the Constitution of the Commonwealth of Massachusetts,51 collected in his Defence of the Constitutions of Government of the United States of America, three volumes of examples and commentaries ... first ten books of Titus Livius on the history of Rome, the sixth book of the Histories of Polybius, and much less importantly, the works of Aristotle, insofar as they anticipate and justify Roman...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
... properties of SmSmad1 and SmSmad1B as described above However, in the presence of SmSmad4 and the active mutant form of TbRI, both SmSmad2 and SmSmad4 interacted with GCN5, indicating the formation of ... from S mansoni Fig Structure of the SmSmad1B gene, cDNA and protein (A) Schematic representations of the SmSmad1B gene, cDNA and protein and the amino acid sequence of SmSmad1B protein Five exons ... of SmSmad2 and SmSmad4 and resulted in the formation of a stable and functional Smad complex [6,7] The above results are consistent with the report that human GCN5 interacts with both TGFb- and...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... mode, Characterization of four pulchellin isoforms and multiplycharged peptide ions (+2 and +3) were automatically mass selected and dissociated in MS ⁄ MS experiments Typical LC and ESI conditions ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... Foundation of Raisiogroup (Raisio, Finland), the Finnish Funding Agency for Technology and Innovation (TEKES) and the Commission of the European Communities, specifically RTD program ‘Quality of Life and ... The met state is the resting state of the enzyme, and in the absence of substrate about 85–90% of the enzyme is in this state [16] Both the met and oxy states of tyrosinases can catalyze the diphenoloxidase ... Da) The identity of this peptide was then confirmed by MALDI-TOF ⁄ TOF fragment ion analysis, where partial sequences of this peptide were obtained from the ladders of b-fragment and y-fragment...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... stresses used for treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O) Panel M is the control ... Figure 7A, D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nucleus, and Fig 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respectively ... ORF of 678 bp, a 5¢ UTR of 131 bp and a 3¢ UTR of 163 bp including 20 bp of poly(A) tail The PsCBL ORF encodes a protein of 225 amino acid residues with a calculated molecular mass of 25.9 kDa and...
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Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
... ENDGAPS, off; NOPGAPS, off; NOHGAPS, off Graphical output of the bootstrap figure was produced by the program TREEVIEW Isolation of RNA and RT-PCR analysis Total RNA was isolated from RTG-2 cells and ... initiation cycle of 15 at 95 °C, amplification cycles of 0.5 at 94 °C, 0.5 at 50 °C and 0.5 at 72 °C, and one termination cycle of at 72 °C, with 35 cycles in total for HSF1a and HSF1b and 30 for heat ... HSF1 isoforms form both homo- and heterotrimers, there are four potential assemblies of HSF1 trimer, namely, two homotrimers (a3 and b3) and two heterotrimers (a2b1 and a1b2) The existence of the...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008