gene protein and function

Báo cáo y học: "Relationship between the tissue-specificity of mouse gene expression and the evolutionary origin and function of the protein" ppsx

Ngày tải lên : 14/08/2014, 14:21

... and transporter proteins (b) The regulatory group includes signal transduction and transcription regulation proteins The pre-metazoa group includes universal proteins and eukaryote specific proteins ... proteins to one of four functional categories: two regulatory categories (signal transduction and transcription regulation) and two metabolic categories (enzymes and transporters) Next, the proteins ... transduction proteins and 5% transcription regulation proteins The phyletic composition of proteins in a tissue is found to be approximately 25% universal proteins, 40% eukaryoticspecific proteins,...

9
391
0

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

Ngày tải lên : 14/02/2014, 19:20

... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned into ... study revealed the structural and functional determinants of the protein protein interaction between v-KIND and MAP2 (i.e the MAP2-binding core module in v-KIND and the v-KIND-binding core module ... interaction between v-KIND and MAP2, as well as its biological signiﬁcance, remains unclear In the present study, we determined the structural and functional properties of the protein protein interaction...

11
658
0

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Ngày tải lên : 15/02/2014, 01:20

... PRTFDC1 (A) monomer and (B) tetramer generated using symmetry-related molecules Interactions with GMP (C) GMP, phosphate and calcium ions are shown as sticks and spheres colored pink and black, respectively ... S-(dimethylarsenic)cysteine Library ﬁles and coordinates for GMP and S-(dimethylarsenic)cysteine were obtained from the HIC-UP ligand database [36] and generated at the prodrg server [37] All ... Environment, Agricultural Sciences and Spatial Planning (to L.W and S.E.), the 4928 Swedish Research Council (to H.E and P.N.) and the Swedish Cancer Foundation (to H.E and P.N.) The Structural Genomics...

11
770
0

Tài liệu Báo cáo khoa học: Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium ppt

Ngày tải lên : 18/02/2014, 14:20

... between R81 and P82 and between G90 and I91 A similar cis peptide between G93 and V94 is found in Tm SurE also Overall structural features of the protein St SurE is an aba sandwich protein and adopts ... and an attempt was made to understand the molecular basis of the lower thermal stability 5856 Results and Discussion Preliminary characterization and crystallization of the protein The surE gene ... archaic and thermophilic homologues The St SurE monomer consists of 13 b-strands, six a-helices and three 310-helices The core of the protein is made up of a nine-stranded b-sheet ﬂanked by a1, a5 and...

10
553
0

Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

Ngày tải lên : 18/02/2014, 14:20

... PIN domain (D141A and D226A) was generated by sitedirected mutagenesis (Stratagene, Cedar Creek, TX, USA) A genetic construct overexpressing recombinant MCPIP with ﬂuorescent red protein (pmaxFN-MCPIP-RFP) ... appearance of functional proteins As reported by Kaspar and Gehrke [18] and Dinarello [19], stimulation of macrophages by mild stimulants (such as C5a complement or some modiﬁed proteins) leads ... lanes 2,3 and 4, lower band) The levels of mutant and wild-type MCPIP mRNAs were higher when these vectors were co-transfected with a vector overexpressing IL-1b (Fig 5, lanes and 4, upper bands)...

14
598
0

Tài liệu Báo cáo khoa học: The Mycobacterium tuberculosis membrane protein Rv2560 ) biochemical and functional studies pdf

Ngày tải lên : 18/02/2014, 16:20

... preimmune and post-third inoculation sera against a M tuberculosis sonicate (lanes 1, and 5) and a membrane fraction (lanes and 4) Lanes and 3, absence of recognition of M tuberculosis proteins ... characterization number of membrane proteins with an as yet undetermined function [11–13] A previous study has shown the importance of the melA gene (encoding an Rv2560 homologous protein) in Mycobacterium ... encoding gene and its transcripts in the M tuberculosis complex and clinical isolate strains, as well as the characterization of the high-activity binding peptide (HABP) involved in the binding to and...

13
492
0

Tài liệu Báo cáo khoa học: Globin gene family evolution and functional diversiﬁcation in annelids ppt

Ngày tải lên : 19/02/2014, 02:20

... structural and functional convergence between circulating and noncirculating intracellular globin and extracellular globin genes in annelids: these genes probably evolved from a common globin-like gene ... ancestor and did not emerge independently of unrelated genes All the annelid intracellular globin gene structures reported here show the same gene structure, whereas Aph aculeata nMb gene lacks ... 2645 Functional diversiﬁcation in annelid globin family X Bailly et al Fig Bayesian phylogenetic tree based on annelid extracellular and intracellular globins (circulating and noncirculating) and...

12
594
0

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

Ngày tải lên : 21/02/2014, 00:20

... in Asian and Mediterranean countries [15] This paper describes the cloning and functional characterization of an AK gene from a genomic library of L donovani as well as its expression and molecular ... active and contained over 99% of the AK protein Finally, the recombinant protein was puriﬁed to apparent homogeneity by afﬁnity chromatography on Ni–NTA agarose columns The recombinant protein ... (http://www.genedb.org/genedb/leish/ index.jsp) The L major AK gene CHR34_tmp.344, corresponds to the L donovani AK2 gene described in this paper The amino acid sequence of L donovani AK2 gene shows...

9
487
0

Báo cáo khoa học: Evolutionary changes to transthyretin: structure and function of a transthyretin-like ancestral protein doc

Ngày tải lên : 07/03/2014, 00:20

... present and discuss recent ﬁndings regarding the identiﬁcation and distribution of TLP genes in nature, the structural and functional characterization of the TLP from various organisms, and the ... of a TLP gene (see Table 1) [11] In these cases, one gene encodes a cytoplasmic TLP and the ‘additional’ TLP gene encodes a periplasmic protein that, similarly to enterobacterial TLP genes, is ... 5-HIU and second to decarboxylate OHCU to (S)-allantoin As previously discussed, bacterial TLP genes are frequently found in close proximity to the uricase gene and to another gene encoding proteins...

13
390
0

Báo cáo khoa học: Glutamic acid residues in the C-terminal extension of small heat shock protein 25 are critical for structural and functional integrity pptx

Ngày tải lên : 07/03/2014, 04:20

... Structure and properties of the small heat shock proteins (sHsp) and their interaction with cytoskeleton proteins Biochemistry (Mosc) 67, 511–519 Macario AJL (1995) Heat-shock proteins and molecular ... B (2005) Protein ﬂexibility and rigidity predicted from sequence Proteins 61, 115–126 49 van Montfort R, Slingsby C & Vierling E (2002) Structure and function of the small heat shock protein ⁄ ... against ordered and disordered protein aggregation FEBS J 275, 935–947 Kumar MS, Kapoor M, Sinha S & Reddy GB (2005) Insights into hydrophobicity and the chaperone-like function of aA- and aB-crystallins:...

14
417
0

Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

Ngày tải lên : 07/03/2014, 12:20

... cDNA Lanes in panels A and B received 4.5–5.5 lg of protein and lanes in panels C and D received lg of protein Arrow, p26 M, molecular mass markers of 97, 66, 45, 31, 21 and 14 kDa 1022 COS-1 ... ubiquitination, degradation and aggregation Hum Mol Genet 13, 703–714 MacRae TH (2000) Structure and function of small heat shock ⁄ a-crystallin proteins: established concepts and emerging ideas Cell ... formation depends upon contact of b-strand 10 from the C-terminal extensions of monomers A and D with b-strands and in the a-crystallin domain of monomers C and B, respectively (Fig 8) A more prominent...

15
515
0

Báo cáo khoa học: Identiﬁcation and functional characterization of a novel barnacle cement protein pptx

Ngày tải lên : 16/03/2014, 05:20

... cement If the protein is present in the protein fraction from the calcareous base and not in that from the peripheral shell, this would conﬁrm that the protein is a cement component and not a component ... analysis of the primary cement, and of the protein fractions in the calcareous base and periphery, indicated that Mrcp-19k was present in the primary cement and protein fraction in the calcareous ... cirri, thorax, prosoma and hemolymph, and the lower part included the mantle, muscle, ovariole, cement gland and hemolymph Twenty micrograms of RNA was electrophoresed and transferred to a Hybond-N+...

11
488
0

Báo cáo khoa học: The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis ppt

Ngày tải lên : 16/03/2014, 05:20

... there is a high degree of sequence similarity and structural conservation of these genes and gene products, confirmation of the true identity and function of these neuropeptides remains difficult ... that there are at least four or five CHH-like genes in M ensis These genes may share high sequence similarity and ⁄ or analogous function Some of these genes may be expressed in FEBS Journal 274 ... development and reduce oocyte size, cause a decrease in total ovary protein incorporation, and suppress ovary total protein synthesis [11,17,18] Biological assays using these criteria are nonspecific and...

11
546
0

Báo cáo khoa học: A (1ﬁ3)-b-D-glucan recognition protein from the sponge Suberites domuncula Mediated activation of ﬁbrinogen-like protein and epidermal growth factor gene expression pot

Ngày tải lên : 16/03/2014, 16:20

... (1fi3)-b-Dglucan-binding protein; subsequently, a gene encoding a fibrinogen-like protein, and also one for a cytokine, are strongly expressed Materials and methods Chemicals and enzymes The sources of chemicals and ... d), for h, and then washed and probed with biotin-curdlan (cur), as described in the Materials and methods (1fi3)-b-D-glucan-binding proteins and was therefore termed GLUBPp_SUBDO The protein comprised ... curdlan Fig Steady-state expression of the SDGLUBP gene [(1fi3)-b-Dglucan-binding protein] , the SDFIBI gene (fibrinogen-like protein) and the SDEGFI-PREC gene (epidermal growth factor EGF precursor)...

14
299
0

Báo cáo khoa học: Conformational and functional analysis of the lipid binding protein Ag-NPA-1 from the parasitic nematode Ascaridia galli potx

Ngày tải lên : 16/03/2014, 18:20

... a-helices and 12% b-turns (Fig 1) Incubation of the protein with different ligands such as palmitate, caprylic and arachidonic acids and retinol in concentrations sufficient to saturate the protein ... helminth lipidbinding proteins offload their ligands to membranes: differential mechanisms of fatty acid transfer by the ABA-1 polyprotein allergen and Ov-FAR-1 proteins of nematodes and Sj-FABPc of ... lumbricoides: sequence polymorphism, stage and tissuespecific expression, lipid binding function, and protein biophysical properties Parasitology 120, 211–224 Poole CB, Grandea AG, Maina CV, Jenkis RE, Selkirk...

10
501
0

Báo cáo khoa học: Spermosin, a trypsin-like protease from ascidian sperm cDNA cloning, protein structures and functional analysis doc

Ngày tải lên : 17/03/2014, 11:20

... band of 28 kDa under reducing conditions and two bands of 33 and 40 kDa under nonreducing conditions 2-ME, 2-mercaptoethanol (B) The N-terminal amino-acid sequences of three bands The 28-kDa protein ... fusion proteins (A) GST fusion proteins including L1, L2, and L1(DL2) (B) The fusion proteins were expressed in clones generated by PCR from the spermosin cDNA as described in Materials and methods ... designated L1), and the 33-kDa protein consisted of the heavy chain (residues 130±388) and a light chain designated as L2 (residues 97±129) There were no distinct bands of L1 and L2 chains on...

7
493
0

Báo cáo khoa học: Bacterial IscU is a well folded and functional single domain protein pdf

Ngày tải lên : 23/03/2014, 12:20

... iscSUA-hscBA-fdx gene cluster from Azotobacter vinelandii J Biol Chem 273, 13264–13272 Kispal, G., Csere, P., Prohl, C & Lill, R (1999) The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis ... the CONTIN, SELCON and CDSSTR methods [22] Near UV spectra required a mm path length cuvette and a 490 lM protein concentration Variations of the CD signal were studied as a function of temperature ... frequency) using standard pulse sequences [26] and analyzed using NMRPIPE/NMRDRAW or XEASY For T1 and T2 measurements, peak intensities were determined for 109 amide resonances as a function of the...

8
303
0

Báo cáo khoa học: N1 – IUBMB 50th Anniversary Symposium: Protein Structure and Function potx

Ngày tải lên : 23/03/2014, 15:20

... hypothetical protein similar to PA-IIL and coded by the gene cv1741 was found The gene was cloned and the recombinant protein CV-IIL, the product of cv1741, has been expressed in E coli and puriﬁed ... in detoxiﬁcation Two distinct supergene families encode proteins with GST activity; at least 16 genes encode proteins expressed in tissue cytosols and six genes are expressed in membranes Cytosolic ... diabetic human placenta and then to purify and examine kinetic properties The fresh placenta was obtained and the tissues were cooled, washed and perfused and weighed, then minced and suspended in three...

64
544
0

Báo cáo Y học: Structural and functional characterization of a C-type lectin-like antifreeze protein from rainbow smelt (Osmerus mordax) potx

Ngày tải lên : 24/03/2014, 03:21

... modulate protein half-life, and facilitate protein protein interactions [28–31] To examine possible roles of the N-linked oligosaccharide of smelt AFP in native protein structure and function, ... untreated and deglycosylated smelt AFP were digested with Glu-C and trypsin in the presence and absence of Ca2+ Both enzymes generated some proteolysis near the N-terminus and/ or C-terminus of the protein ... (1996) Antifreeze proteins: structures and mechanisms of function Chem Rev 96, 601–617 Ewart, K.V., Lin, Q & Hew, C.L (1999) Structure, function and evolution of antifreeze proteins Cell Mol...

8
518
0

Báo cáo khoa học: Cloning and functional analysis of 5¢-upstream region of the Pokemon gene pptx

Ngày tải lên : 30/03/2014, 04:20

... tumor suppressor gene and is a central regulator in oncogenesis Overexpression of the Pokemon gene can decrease expression of the ARF gene, which in turn results in p53 degradation and oncogenic ... Pokemon gene both inhibited oncogene-mediated cellular transformation and induced cell senescence and apoptosis [22,26] Therefore, Pokemon plays a crucial role in cell tumorigenesis and may be ... sequences (254 and 106 bp) between the NEGU and NEG-D elements The distance between the NEG-U and NEG-D elements was 253 and 106 bp in D-254 and D-106, respectively The right-hand panel shows...

14
340
0