... an SNP were 60.0 68.0 detected with the primer premier 5.0 software to design the genotyping protocols Genotyping of other individuals ofthe second and third generations were carried out by PCR-RFLP ... present in heart, one (VLDLR-a) with an O-linked sugar domain and the other (VLDLR-b) without The prediction results from the Swiss Institute of Bioinformatics software showed that the VLDLR-a ... that the differential expression of both VLDLR variants varies slightly among species and tissues, the roles of each isoform may differ In addition, the fact that the transcript lacking the O-linked...
... with the same genes being induced among them The striking difference in number of genes induced between the q12 × (orange) and the q12 × (blue) cohorts strongly emphasizes the importance ofthe ... exemplified by the blue cohort signature, which clumped together not only the samples from the blue group, which served as a basis to select the genes used for clustering, but also ofthe other 15 imiquimod-treated ... during the early stages of therapy in lesions whose transcriptional profiles were sufficiently activated The relatively low sensitivity ofthegene set as predictors most likely reflects the exclusion...
... previously reported the structures ofthe mouse a3 integrin subunit gene including the exon/intron organization and the alternative exon usage for the generation of variants ofthe a3 subunits (a3A ... 324 bp upstream ofthe major transcription start site The presence of a CCAAT box and the absence of a TATA box seem to be one ofthe characteristics ofthe mouse integrin a3 gene We identified ... promoter region The presence of a CCAAT box seems to be a characteristic feature ofthe a3 integrin gene among integrin a subunit genes Fig Flow cytometric analysis ofthe expression ofthe integrin...
... that one ofthe two other known members ofthe family, namely the iGb3 synthetase and the Forssman synthetase, could have a dual specificity Alternatively, there could exist another gene in the rat ... Table and Fig There was a general agreement between the presence ofthe A antigen and that ofthe Abo gene mRNA Indeed, the A antigen was expressed in the oesophagus, the stomach, the large intestine ... been generated in the 1940s and is inbred Therefore, in these animals, the B antigen cannot be synthesized by the enzyme product of an allele at the Abo locus It is to be expected that another gene...
... vertebrates) One example of loss of a core gene is the case ofthe Toll receptor in Hydra magni apillata p http://jbiol.com/content/8/10/89 Whereas Nematostella and other members ofthe basal cnidarian ... Many of these taxonomically restricted genes are paralogs or highly diverged members of large gene families, generated by duplication events that have occurred at all levels On the basis ofthe ... evolution, whereas the beetle Tribolium and the honeybee Apis have lower rates of evolution and have lost fewer ofthe ancient genes present in Urbilateria Although vertebrates in general have lost...
... expense of ex vivo gene transfer remain Furthermore, the occurrence of insertional mutagenesis during human gene therapy trials [11] has generated a huge barrier to the use of oncoretroviruses ... synthesis [42] The need for the latter can be obviated with the use of scAAV, and recent findings confirm the superiority of these vectors in the rabbit knee joint [18] According to data from the ... experimental models of OA [79-82] Nearly all of these have used IL-1Ra as the transgene product, reflecting the importance of IL-1 as a mediator in the osteoarthritic joint The equine study of Frisbie...
... M., 1981 Thegene map ofthe rabbit I Synteny ELLIN ENNE ILLOIS between the rabbit gene loci coding for HPRT, PGK, G6PD, and GLA Their localisation on the X-chromosome Cytogenet Cell Genet., 29, ... 1982 Thegene map ofthe rabbit II Analysis of ENNE ILLOIS ELLIN the segregation of 11 enzymes in rabbit x hamster somatic cell hybrids : two syntenic groups, LDHB-TPI and LDHA-ACP2 Cytogenet ... clones, was therefore always possible clones (fig 1) to determine if the rabbit fragment is present Table shows the correlation analysis ofthe results obtained the cDNA probes with the somatic...
... observability is the degree to which the results of an innovation are visible to the adopters If there are observable positive outcomes from the implementation ofthe innovation then the innovation ... account ofthe study methods are presented elsewhere [9] Questionnaire The theoretical underpinnings ofthe questionnaire were based on Rogers' Diffusion of Innovation theory and the TPB The questionnaire ... using the items: "the kit is easy/simple to use;" "the content ofthe kit is clear;" "the content ofthe kit is relevant" with the same response options as above Trialability was measured using the...
... raised the possibility that the 150-probe set contains genes related to pathogenesis of disease Functional analysis ofthe genes overexpressed in the inflammation group showed that three gene groups ... comparison between these two types of biopsies due to the unavailability of tissues Further, the isolation of RNA from a liver biopsy fragment may limit the potential implication ofthe findings with ... makeup Conclusions Gene expression profilingofthe liver at the time of diagnosis of biliary atresia identifies prominent signatures of inflammation or fibrosis in most patients These signatures...
... observability is the degree to which the results of an innovation are visible to the adopters If there are observable positive outcomes from the implementation ofthe innovation then the innovation ... account ofthe study methods are presented elsewhere [9] Questionnaire The theoretical underpinnings ofthe questionnaire were based on Rogers' Diffusion of Innovation theory and the TPB The questionnaire ... using the items: "the kit is easy/simple to use;" "the content ofthe kit is clear;" "the content ofthe kit is relevant" with the same response options as above Trialability was measured using the...
... alignment of aa 296 - 735 region in all of those four Thai-PRRSV nsp2 sequences Then, the remaining nsp2 sequences of other Thai PRRSVs (78/51, 8NP59, 01CS1/2, 8NP46, 8NP147 and 8NP148) were further genetically ... found in of those partial nsp2 sequences Therefore, out of 10 Thai PRRSVs in this study had a nucleotide deletion in the nsp2-coding region (or at least in the studied region) The size ofthe partial ... introduction of new exotic PRRSV strains from other countries has been limited to a minimum Our data did not support the hypothesis ofthe introduction of new PRRSV strains with the same nsp2...
... lineage On the basis of these findings, the most critical choice of an animal model for investigation should be based on anatomical and mechanical considerations ofthe spinal Page of unit rather than ... out that the results of Minogue and colleagues [1] differ from those of Gilson and colleagues [13], who have reported that the expression of cytokeratin was restricted to a small cohort of cells ... (described as notochordal) in the adult bovine nucleus pulposus Surprisingly, these cells were similar in size to the chondrocyte-like cells ofthe nucleus pulposus and unlike the large notochordal cells...
... gel, and were added to the list of potential proteins of interest All 166 spots were automatically matched by the software to the Sypro stained image ofthe preparative gel Ofthe 166 spots excised, ... Image ofthe Sypro stained preparative gel The horizontal axis represent pH, here ranging from on the left to 10 on the right, and the vertical axis represent molecular weight A total of 500 μg of ... relevant roles of mesenteric lymph in the progression of T/HS as suggested by the identification of numerous proteins that either increase or decrease in the post-shock state The DIGE technique...
... (that is, they were designed to the same region ofthe gene) and 2,413 ofthe probes are designed to different parts ofthe same gene model As shown in Figure 8a, for the probes designed to the same ... changes in gene expression Nonetheless, for the lines examined here, the ND101/W23 hybrid is more similar to W23 than the heterologous A619 line The complete results from the analysis ofthe common ... metabolism (GO:6139) On the other hand, pollen-specific genes are enriched in pectin esterase interactions Enrichment ofGene Ontology classes There is currently no official release ofGene Ontology (GO)...
... long; the 5' ends in more than 80% ofthe reads of those sequences in the cluster ofthe dominant sequence ofthe pairs had to be consistent with each other After these criteria were met, we then ... RNA transcriptomes ofthe two phases show big differences in their length distribution and composition Classification ofthe rest ofthe small RNAs The rest ofthe sequences in the locust small ... file 3) Their features indicated that these 26-29-mer small RNAs might be ofthe piRNA class of small RNAs Therefore, we analyzed the sequences of these small RNAs to look for the presence of an...
... regard to the size of introns and 3’ untranslated region (3’ UTR; Figure 3) Compared to the 0.3 – 0.35 kb size of intron ofthe LHβ genes, the size ofthe corresponding intron in the FSHβ genes is ... that ofthe LHβ geneThe tilapia LHβ amino acid sequence shares the highest homology with the grouper at 96%, while the highest homology ofthe tilapia FSHβ amino acid sequence is with that ofthe ... 2002) The degree of stimulatory effects of GnRH on the gonadotropin genes is dependent largely on the reproductive stage ofthe fish and the species, and to a lesser extent, the gender In the study...
... represent the 5¢ ends ofthe mature fabp3 gene transcripts By aligning the sequence ofthe 5¢ RLM-RACE products with the fabp3 gene sequence, the transcription start sites ofthe zebrafish fabp3 gene ... Southern analysis ofthe zebrafish fabp3 gene DNA sequence and structure ofthe zebrafish fabp3 gene Using a pair of primers flanking the entire coding region and a portion ofthe 3¢ UTR for the zebrafish ... 3225 Fig Nucleotide sequences ofthe cDNA and the 5¢ upstream region of zebrafish fabp3 gene (A) The 5¢ upstream region of zebrafish fabp3 geneThe coding sequence ofthe first exon is shown in uppercase...
... Cttarget gene - Ctactin; Ct values then were obtained by subtracting the Ct of a reference sample from the Ct ofthe studied samples Finally, the levels of expression ofthe target genes in the studied ... ofthe genes of this panel, whereas the combination of TNF- and IL-1 had a significant stimulatory effect on the whole set of genes tested (Figure 6) Notably, the effects ofthe combination of ... ofthe scaled data for elimination ofthe genes with a flag absent in more than half ofthe samples and selection ofthe 8,000 genes that displayed the widest inter-individual variations in the...
... partners On the other hand, we hypothesize that further insights into the molecular basis ofthe developing heart will point towards novel candidate genes for a specific CHD type based upon the phenotypic ... executes these components when the page is rendered, or they can explicitly be called from within the page through the use of a specific tag For example, to include the list of CHDs linked to JAG1, the ... JAG1, the generic extension first calls the chdsForGenes component with parameter JAG1 to retrieve the data in the form of (variable, value) pairs Second, the extension retrieves the chdsForGeneTemplate...
... expression of >90% of previously uncharacterized genes on these lists (Table 1) We therefore conclude that these 'panneural' profiles provide accurate representations ofgene expression in the C elegans ... indication ofthe complex signaling capacity ofthe C elegans nervous system, most ofthe known peptide neurotransmitter genes (for example, 20 of 23 FMRFamide genes or 'flps') are enriched in the larval ... relative lack of enrichment of homeodomain mRNAs in the embryonic pan-neural profile was initially surprising given strong genetic evidence for the widespread role ofthe members of this transcription...