gene expression and epigenetic signatures of germ cell derived pluripotent stem cells and embryonic stem cells
Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... gene therapy 21 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells 1.5 Stem ... pluripotent stem cell derived- neural stem cell human induced pluripotent stem cell derived- neural stem cell expressing CodA human induced pluripotent stem cell derived- neural stem cell expressing ... sequences for pluripotent stem cell “stemness”-specific and neural stem cell- specific markers 57 Table Changes in expression of embryonic stem cell- specific markers and embryonic stem cell differentiation/lineage...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... c-myc gene expression To investigate whether NAMI-A-induced inhibition of ERK1/2 signaling could affect the expression of a cell growth-related gene, we assessed c-myc gene expression in growing cells ... mRNA expression in PD98059-treated, serum-cultured cells Since both PD98059 and NAMI-A could not completely inhibit c-myc gene expression in serum-cultured cells, it is possible that protooncogene ... endothelial cell plays a crucial role in angiogenesis [39] and that it might affect the growth and differentiation of neighboring cells [40] The endothelial cell is also the target cell for tumor...
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Báo cáo khoa học: Inhibition of urokinase receptor gene expression and cell invasion by anti-uPAR DNAzymes in osteosarcoma cells pot
... Inhibition of uPAR by DNAzymes all of which contribute to focal proteolysis and regulation of wound healing, angiogenesis, and metastasis [5] Of the components of the uPA system, uPAR is of particular ... control and mutant DNAzyme-treated cells (Fig 5) Inhibition of Saos-2 cell invasion by Dz720 The plasminogen activator system can promote the breakdown of ECM and facilitate cell invasion and metastasis ... blue and visualized by microscopy (A) Untreated cells (B) Cells transfected with Dz720 mutant (C) Cells transfected with Dz720 active (D) Graphical representation of toluidine blue-stained cells...
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Báo cáo y học: "Abnormalities of B cell phenotype, immunoglobulin gene expression and the emergence of autoimmunity in Sjögren’s syndrome" pdf
... Memory B cells 60% BBcells cells B cells CD27 Expression CD27 Expression Plasmablasts Naïve Naïve B cells B cells Memory Memory B cells B cells CD27 Expression Schematic distribution of B cell subsets ... peripheral naive (IgM+/CD27–) and memory (CD27+) B cells Interaction of CD27 with its ligand on T cells, CD70, serves as a pathway of differentiation of B cells into plasma cells [73,75,76] Recently, ... (founder) B cells or from an early state of dark-zone germinal center cells [71] However, it is uncertain whether these cells are able to leave the germinal center Accumulation of memory-type B cells...
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Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
... addition to the loss of cell cell adhesion, involves degradation of the extracellular matrix and release of cells from the constraints of cell cell and cell matrix interaction MMPs and urokinase-type ... potent inducer of uPA gene expression [16] d Fig Effects of Decma treatment on E-cadherin distribution, cell scattering and uPA expression (A) T47D cells (a and b) and MCF7 cells (c and d) were ... that the expression of E-cadherin and the expression of MMPs are inversely correlated [11,12] and that E-cadherindependent cell cell contact regulates the expression of MMPs and uPA in vitro [13–15]...
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Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx
... promoter region of pRIBOTEX and related vectors induces the transcription of genes cloned downstream of the promoter and their chromosomal integration [19] After 48 h of transfection, geneticin (G418) ... the parasite genome organization Results and Discussion Expression of the oat ADC gene in T cruzi In order to study the expression of the foreign ADC gene in T cruzi epimastigotes, we transformed ... deletion mutant for ODC and ADC genes, we used these polyamine auxotrophic parasites as recipients of foreign ODC or ADC genes to study their expression and the eventual suppression of polyamine auxotrophy...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... primary cells and animal models but, as a preliminary step, they unravel one of the mechanisms of TNFamediated withdrawal of insulin action in HepG2 cells Results Incubation of HepG2 cells with ... cytosol of insulin-treated cells, but was largely absent in control cells However, when cells were pretreated with TNFa (1 nm, 24 h) prior to insulin incubation, this nuclear extrusion of Foxa2 and ... treatment (50 nM, 15 min) Cells incubated in the absence of any of these were taken as the control (Con) On termination of incubation, cells were lysed and the nuclear (50 lg) and cytosolic (40 lg)...
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Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx
... sample and of pBR322 isolated from cells incubated for 30 with 0.1 mgámL)1 of coumermycin and 0.2 mgámL)1 rifampicin Topoisomerase concentration The topoisomerase I and DNA gyrase content of the cells ... of gyrase activity and gyrase expression for supercoiling are negative (i.e the activity and expression of DNA gyrase is inhibited not stimulated by higher levels of supercoiling) and those of ... Accordingly we measured the cellular concentration of DNA gyrase at the various expression levels of topoisomerase I However, over the thousand-fold range of expression levels of topoisomerase I the...
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Báo cáo khoa học: Glutamine stimulates the gene expression and processing of sterol regulatory element binding proteins, thereby increasing the expression of their target genes docx
... activities of SREBP targets HepG2 cells (A and B) and Caco-2 cells (C) were transfected with 200 ng of the reporter constructs consisting of the indicated gene promoters and 200 ng of pEF-b-Gal The cells ... HepG2 cells and Caco-2 cells were plated in 12-well plates at a density of 1.0 · 105 cells well)1, cultured with DMEM for 20 h, and then transfected with 200 ng of one of the reporter plasmids and ... the gene expression of SREBP family members, SREBP-1 and SREBP-2 SREBP-1 exists in two forms, designated 1a and 1c SREBP-1a is the predominant isoform in most cultured cells, including HepG2 cells...
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Báo cáo khoa học: A functional polymorphism at the transcriptional initiation site in b2-glycoprotein I (apolipoprotein H) associated with reduced gene expression and lower plasma levels of b2-glycoprotein I docx
... (lanes 1, and 4) Effect of the )1CfiA mutation on reporter gene expression To further confirm that the )1CfiA mutation is associated with low expression of the b2GPI gene, we performed Fig Effect of the ... [30,31] However, our in vitro mutagenesis and expression study did not link these mutations to an altered b2GPI gene expression [32] Although, in vitro mutagenesis and expression study not rule out ... 4.3 and 7.3 mgÆdL)1) This indicates that the genetic basis of APA is complex and other genetic and/ or biological factors are involved in the occurrence of APA The structural organization of the...
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báo cáo hóa học: " Pro-inflammatory gene expression and neurotoxic effects of activated microglia are attenuated by absence of CCAAT/enhancer binding protein b" potx
... medium and aliquots of the cell suspension (10 μL/well) were seeded on top of DIV primary neuronal cultures at a final density of × 10 cells/ mL (1.3 × 105 cells/ cm2) Straccia et al Journal of Neuroinflammation ... the anti-inflammatory genes IL-4 and TGFb1 Absence of C/EBPb results in significant decreases in LPS-induced expression of NOS2 and in LPS+IFNg-induced expression of NOS2 and IL-1b Results are ... colocalization of NOS2-positive cells and CD11b-positive cells Bar = 50 μm Straccia et al Journal of Neuroinflammation 2011, 8:156 http://www.jneuroinflammation.com/content/8/1/156 Figure Lack of C/EBPb...
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Báo cáo hóa học: " Inhibition of cytokine gene expression and induction of chemokine genes in non-lymphatic cells infected with SARS coronavirus" doc
... cytokine storm and the severe and potentially fatal respiratory distress which is the hallmark of full-blown SARS Methods Cells and viruses Simian VeroE6 cells, human Caco-2 cells and human embryonic ... activated IP-10 and RANTES expression in HEK 293 cells as expected (Fig 4B, upper and lower panels) Thus, SARS-CoV induces IP-10 gene expression in Caco-2 cells, but not in HEK 293 cells, again ... the Caco-2 cells and the low passage HEK 293 cells as useful systems for studying the influence of SARS- CoV on the immune system-independent induction of cytokines Interferon genes and their...
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báo cáo hóa học:" Gene Expression and Pathway Analysis of Ovarian Cancer Cells Selected for Resistance to Cisplatin, Paclitaxel, or Doxorubicin" docx
... ECM and other genes altered in cisplatin derived resistant cells B Network including proteasome genes and other genes altered in doxorubicin resistant cells C Network containing translation genes ... OV90P cells compare to the OV90 cells For the other genes analyzed, such as the GAGE family genes, CD96, and VSIG1, the expression levels were increased significantly in various drug resistant cells ... contrast, the doxorubicinderived resistant cells (OV90D-6 and OV90D-7) exhibited significant cross-resistance to paclitaxel, and the paclitaxel -derived resistant cells (OV90P-3 and OV90P-7) were resistant...
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Báo cáo toán học: "Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an Anti-Estrogen Effect of Iodine" doc
... the changes in gene expression identified by microarray analysis RNA was isolated from control cell and cells grown in the presence of mM iodine for 48 hours QRT-PCR analysis of genes predicted ... no effect on cell proliferation or viability, relative to control cells However, treatment with mM iodine/iodide was toxic to the cells, inhibiting cell proliferation and reducing cell viability ... analyzed the effects of Lugol’s iodine solution (5% I2, 10% KI) on global gene expression in the estrogen responsive MCF-7 breast cancer cell line Analysis of the gene expression profile was used to...
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Báo cáo y học: "Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis" pptx
... status of the chondrocyte is central to our understanding of the initiation and progression of osteoarthrits (OA) [16] An initial anabolic response of chondrocytes in OA includes an upregulation of ... cartilage undergoing OA degeneration or are more generalized within arthritic joints An understanding of the changes that occur with the onset and progression of cartilage degeneration in R853 OA ... 0.01) and lumican (14.6-fold; P < 0.01) In the same region there were downregulations of decorin (1.6-fold; P < 0.01) and CTGF (2.1-fold; P < 0.05), and unchanged expression of fibromodulin and...
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Báo cáo y học: "The protective effect of licofelone on experimental osteoarthritis is correlated with the downregulation of gene expression and protein synthesis of several major cartilage catabolic factors: MMP-13, cathepsin K and aggrecanase" docx
... number of cells and the number of cells that stained positive for the specific antigen were determined The results were expressed as the percentage of cells that stained positive for the antigen (cell ... in situ effect of licofelone on the gene expression and protein synthesis of the major collagenolytic enzymes (MMP-13 and cathepsin K) and aggrecandegrading proteases (ADAMTS-4 and ADAMTS-5) in ... exists between the mRNA and protein levels At the two dosages tested, the R1094 ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis The level of gene expression of ADAMTS-5 in OA cartilage...
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Báo cáo y học: "The influence of the NOD Nss1/Idd5 loci on sialadenitis and gene expression in salivary glands of congenic mice" docx
... data are robust and provide clear differences between the strains Gene expression differences of genes located in the NOD loci Idd5 and Nss1 The main focus of the gene expression profiling has been ... loci affect the gene expression profiles in the SGs of the congenic mice The gene expression reflected the congenic fragments When comparing the gene expression of the B10.Q.Nss1 and the B10.Q.Nss1/Idd5 ... were selected from the gene list of the standard analysis, and Zap70, Cd19, Sell, Dock7 and Fas were selected from the gene list of the alternative analysis ference in gene expression This was convincingly...
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Báo cáo y học: "Gene expression and activity of cartilage degrading glycosidases in human rheumatoid arthritis and osteoarthritis synovial fibroblast" potx
... Gene expressions were characterized in SFs and in SMs from RA patients and OA patients Figure Gene expression of Hc-gp 39 was orders of magnitude higher than that of any of the other tested genes ... Effect of cytokines and nitric oxide on expression and secretion of glycosidases by synovial fibroblasts We tested the effect of various cytokines and nitric oxide on the gene expression of glycosidases ... 100% As shown in Figures 5a and 6a, TGF-β1 has significantly downregulated the expression of HexA and HexB genes, as well as of GusB and Hc-gp 39 The suppression of gene expression was more pronounced...
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Báo cáo y học: "JNK pathway is involved in the inhibition of inflammatory target gene expression and NF-kappaB activation by melittin" ppsx
... inflammatory gene expression, iNOS and COX-2 expression was determined The inhibitory effect of melittin and bee venom on iNOS and COX-2 expression by LPS and SNP in Raw 264.7 cells (Fig 5A) and in ... synoviocytes, and SNP treated Raw 264.7 cells and synoviocytes, but the expression ERK and p38 was also reduced, indicating that blocking of the activation of p38 and ERK was not specific (Fig 1A and 1B) ... induced by LPS or SNP Raw 264.7 cells (A) and THP-1 cells (B) were transfected with pNF-κB-Luc plasmid (5× NF-κB), Raw 264.7 cells or THP-1 cells were pretreated with 10 and 20 μM SP600125 hr prior...
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Báo cáo y học: " Effective inhibition of HCMV UL49 gene expression and viral replication by oligonucleotide external guide sequences and RNase P" pptx
... cells treated with EGS324, whereas a reduction of less than 10% was found in the C-EGS324 treated cells Inhibition of UL49 expression is not expected to affect the expression of other viral genes ... exogenously into human cells and inhibit cellular gene expression [28] Our study showed that exogenous administration of DNA-based EGSs is highly efficient in inhibiting gene expression and viral growth ... class of agents that may lead to highly effective and specific inhibition of gene expression RNA-based EGSs have been expressed endogenously as transgenes in both bacteria and mammalian cells...
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