gene delivery methods for reprogramming somatic cells
Báo cáo sinh học: " Improved gene delivery to human saphenous vein cells and tissue using a peptide-modified adenoviral vector Lorraine M Work1, Paul N Reynolds2 and Andrew H " pptx
... vector system for gene delivery Adenoviral vectors have proven efficient for gene delivery in this context [9] although high titers are required to provide sufficient levels of gene delivery to ... to vascular cells including intact human saphenous vein allowing lower doses of vector to be used to achieve attractive levels of gene delivery to grafts ex vivo [13] Likewise, gene delivery to ... binding in relation to time is therefore improved through the RGD-4C peptide and has direct implications for the design of gene therapy vectors for use in human CABG gene therapy procedures in the...
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THE DIRECT REPROGRAMMING OF SOMATIC CELLS: ESTABLISHMENT OF A NOVEL SYSTEM FOR PHOTORECEPTOR DERIVATION
... determination of candidate genes for reprogramming, cloning of these candidate genes into appropriate vectors, adaptation of a lentivirus system for gene delivery, generation of cells to use as a high-throughput ... demonstrated a genetic approach to reprogram mouse somatic cells to a pluripotent state via lentiviral delivery of a cocktail of four genes that govern pluripotency [10] He dubbed the cells derived ... neuralization of somatic cells induced by combinations of known pro-neural genes, and finally, the induction of photoreceptor-like phenotypes in somatic cells 2.1 Selection of candidate genes While...
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Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc
... synthesis Generation of longer cDNA fragments from SAGE tags for gene identification (GLGI) To avoid the disadvantage mentioned above in the RAST-PCR method, Chen et al proposed a new method called generation ... 2009 FEBS W.-J Xu et al Methods for 3¢ end amplification from SAGE tags A B Fig Schematic for GLGI (A) In this process, first-strand cDNA synthesized by oligo(dT) is used for PCR In the first cycle, ... only for the subsequent PCR but also for 5¢-RACE, 3¢-RACE and northern blotting, etc Finally, the two-step nested PCR principle can easily produce 3¢-end cDNA tag-specific frag- Methods for 3¢...
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Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx
... cytofectins for gene delivery H3C H3C N CH3 N H3C N O O O NH O NH R R The R group varies with the derivative: C11H23 for dilauroyl (1,3lb1) C13H27 for dimyristoyl (1,3lb2) C15H31 for dipalmitoyl ... analyzed with reference to their gene- delivery FEBS Journal 275 (2008) 148162 ê 2007 The Authors Journal compilation ê 2007 FEBS 157 Novel cytofectins for gene delivery M Spelios and M Savva capabilities ... of enhancing the transport of exogenous genetic material into cells in vitro, and their design eliminates the need for additional components in the gene- delivery system (i.e DOPE and cholesterol),...
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gene delivery to mammalian cells, volume 1
... underlying synthetic vector-mediated DNA delivery With the continuous effort to meet the need for safe and efficient gene- delivery methods for human gene therapy, it is foreseeable that significant advances ... further improvement in gene- delivery efficiency for human gene therapy In attempting to predict what surprises the next 5–10 years might hold for chemical methods for DNA delivery, we believe that ... ϫ 107 cells Cells can be either normally growing suspension cells, for example B -cells, or trypsinized anchorage-dependent cells Pellet cells in a 50-mL conical centrifuge tube Resuspend cells...
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gene delivery to mammalian cells, volume 2
... describe methods for adenovirus-mediated gene delivery to myoblasts, myotubes, and myofibers in vitro, and for intramuscular injection in vivo Although methods are described for gene transfer by ... libraries for screening in mammalian cells have been generated using plasmids, retroviral vectors, or Epstein-Barr virus (EBV)-based vectors From: Methods in Molecular Biology, vol 246: Gene Delivery ... (Wisent Inc.) Use 5% FBS for 293 cells and 10% FBS for all other cell lines Prepare heatinactivated FBS by heating the serum at 56°C for 30 to inactivate complement For all cells lines, cell growth...
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nonviral vectors for gene therapy, methods and protocols
... vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 11 Kabanov, A V and Kabanov, V A (1995) DNA complexes with polycations for the delivery ... sufficient for gene delivery into cells Bioconjugate Chem 10, 773–780 Niidome, T., Urakawa, M., Sato, H., Takahara, Y., Anai, T., Hatakayama, et al (2000) Gene transfer into hepatoma cells mediated ... 2.3 Cell Culture and Gene Transfer into Cells COS-7 cells (a monkey kidney cell line, RCB accession no RCB0539), HeLa cells (a human cervix, RCB accession no RCB0271) and CHO cells (a Chinese hamster...
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Báo cáo sinh học: "Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma" doc
... at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading control HEK293T cells ... as a gene delivery vehicle Among the total non-viral gene vectors, PEI, with high transfection efficiency, has bright prospects in application [9,10] However, PEI is not fit for keeping on gene ... Only about half of cells were alive with 200 μg/ml 2-kD PEI treatment, while 60% cells had viability even exposed to more than 200 μg/ml of FAPEAs or HPEI Generally, for OS-RC-2 cells, the cytotoxicity...
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báo cáo hóa học:" Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma" pot
... at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping gene b-actin was taken as a loading control HEK293T cells ... as a gene delivery vehicle Among the total non-viral gene vectors, PEI, with high transfection efficiency, has bright prospects in application [9,10] However, PEI is not fit for keeping on gene ... Only about half of cells were alive with 200 μg/ml 2-kD PEI treatment, while 60% cells had viability even exposed to more than 200 μg/ml of FAPEAs or HPEI Generally, for OS-RC-2 cells, the cytotoxicity...
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Báo cáo hóa học: " Research Article Computational Methods for Estimation of Cell Cycle Phase Distributions of Yeast Cells" docx
... estimate for the DNA replication function f (t), we can estimate the age distribution of a synchronous population We assume that the function f (t) is the same for all cells, that is, for cells ... population corresponding to (a) cells with no bud, (b) cells with a small bud, and (c) cells with a large bud The number of cells is normalized with the maximum number of cells Only the first cell cycle ... of cells at time t is C(t) = t=0 c(i) = N(2 − 2(1−t) ) That i is, for a given t, C(t) is the total number of cells at the earlier phases of the cell cycle As we know the cumulative number of cells...
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Báo cáo y học: "Gene expression signatures for autoimmune disease in peripheral blood mononuclear cells" potx
... of genes analyzed in the juvenile subjects or basic differences in pathogenesis between childhood and adult forms of these disorders Table Differentially expressed autoimmune genes Category Gene ... yielded sets of genes that were repeatedly detected in the SDA-derived models For RA compared to control genes were identified and for SLE Table Differentially expressed autoimmune genes detected ... of analyzing small blood samples These gene filters are, however, no longer available Current recommendations for other platforms, such as the Affymetrix Gene Chip Arrays®, require no more than...
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Báo cáo y học: "Threshold-free high-power methods for the ontological analysis of genome-wide gene-expression studies." pptx
... (cumulative) distribution functions for the gene- specific differential expression scores x1, ,xN for an N -gene category and the scores x'1, ,x'M for an M -gene reference gene population, respectively ... removes the need for discrete methods Regarding the remaining methods, ZK is slightly Genome Biology 2007, 8:R74 information The total time required for analyzing the 25 studies (all methods and ontologies) ... genes Modulated genes Category genes Cumulative distribution (a) Genome Biology 2007, 0.2 −5 Gene score information Genome Biology 2007, 8:R74 interactions We proceeded to apply all methods to real...
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Báo cáo sinh học: "Genetic parameters for lactation traits of milking ewes: protein content and composition, fat, somatic cells and individual laboratory cheese yield" ppt
... mean of somatic cells (in their natural logarithmic form) Standard errors of genetic correlations ranged from 0.06 to 0.13 0.05 0.30 Genetic parameters for lactation traits in ewes Table IV Genetic ... records for somatic cell count The SCC were determined using the Fossomatic method as described by Gonzalo et al [23] Test day records of somatic cell count were transformed to their logarithmic form ... Primitivo F., Genetic parameters for milk yield in dairy sheep, J Anim Breed Genet 112 (1995) 5963 [13] Da Y., Grossman M., Misztal I., Wiggans G.R., Estimation of genetic parameters for somatic cell...
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Báo cáo sinh học: "The use of retroviral vectors for gene therapy-what are the risks? A review of retroviral pathogenesis and its relevance to retroviral vector-mediated gene delivery" pptx
... oncogene activated in the X-SCID trial, is normally expressed in primitive haematopoietic cells (the target for gene transfer) but not in mature cells (80) Therefore, it will be accessible for ... retroviral genes Oncogene capture The mechanism of oncogene capture appears to be dependent on the generation of a chimeric retroviraloncogene transcript (69, 70) This suggests that the risk of oncogene ... in activation of an oncogene, in combination with the requirement for other genetic changes before a cancer eventuates Although proviral integration can also result in gene inactivation, inactivation...
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Báo cáo sinh học: "In vivo gene targeting of IL-3 into immature hematopoietic cells through CD117 receptor mediated antibody gene delivery" pot
... bone marrow cells Conclusions Our gene delivery system is specific and leads to transient gene delivery and expression It may prove useful and safe for numerous clinical applications of gene transfer ... 40 cells using an inverted microscope Cells were then harvested and studied for IL-3 gene expression Two weeks post-transfection, semi-solid colonies were removed from methylcellulose culture for ... assay × 105 cells from bone marrow were assayed for clonogenic hematopoietic immature cells [23] Briefly, cells were plated in triplicate in 35-mm dishes at a concentration of × 105 cells/ ml in...
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Báo cáo sinh học: "Comparison of four statistical methods for detection of a major gene in a progeny test design" pptx
... several genes having major effects on commercial traits have been identified The dwarf gene in poultry (Merat & Ricard, 1974), the halothane sensitivity gene in pigs (Ollivier, 1980), the Booroola gene ... of simplification and validity Four methods are compared on simulated data ’ METHODS The four methods considered type of test statistics rely upon the same information structure and the same Experimental ... needed for the evaluation of the statistics under H Ten replicates of a sample of 10 sires and 10 progenies per sire used 640 s for the ls statistic, A 142 s for the I statistic and 48 s for the...
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ADVANCED METALLISATION METHODS FOR MONOCRYSTALLINE SILICON WAFER SOLAR CELLS
... used for metallisation of HET cells [38] Metallisation technologies for Si wafer solar cells (including HET cells) are discussed further in Section 2.6 Screen printing for HET cells and plating for ... HET cells In order to metallise HET cells using metal plating, the transparent conductive oxide layer (TCO) for such cells needs to be masked before the plating step Therefore, TCO masking methods ... Screen printing for homojunction Si wafer solar cells typically includes a hightemperature (~800 °C) firing step for contact formation to heavily doped Si regions However, HET solar cells have two...
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COAXIAL DOUBLE WALLED MICROSPHERES FOR DRUG AND GENE DELIVERY APPLICATIONS
... transfection model chosen for the gene delivery experiment High molecular weight bPEI up to 800 kDa has been used for non-viral gene transfer, exhibiting a superior capability to form compact and stable ... are typically very efficient in gene transfer They can be transformed into effective gene delivery systems by replacing part of their genome with a therapeutic gene However, safety concerns remain ... application of viral gene delivery Retroviruses, for instance, suffer from oncogenic risks associated with insertional mutagenesis, leading to the development of malignant cells Moreover, viral...
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Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system
... vectors for gene delivery to the nervous system has been limited by their ability to transfer genes only to dividing cells, although they are well suited for on-site delivery to neural precursors for ... improved gene delivery could be achieved by using these vectors In particular, gene delivery vectors evaluated in this study would be most useful for gene therapy for CNS diseases Nevertheless, the generalizable ... Particles…………141 5.1.2 Genetic Engineering of Baculovirus Vectors for Controlled Gene Expression……………………………………………………… 142 5.1.3 Surface Modifications of Baculovirus Vectors for Improved In Vivo Gene Delivery ………………………………………………...
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