gdp or gtpgammas and incubation with products of the in vitro transcription translation reaction

Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt

Ngày tải lên : 18/02/2014, 06:20

... often transcriptional regulators containing a C2H2 domain for DNA binding, but can also be found in combination with various other protein–protein interaction motifs, such as KELCH or meprin and ... was noted on the basis of homology with the C-terminal region of meprins A and B and the TRAF-C domain, and, like the BTB domain, facilitates protein–protein interaction [24] Meprins are tissue-speciﬁc ... proteins use their BTB ⁄ POZ domain to interact with the cullins CUL3A and CUL3B [3,4,16], we investigated what kind of protein–protein interactions were facilitated by their MATH domains by performing...

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Báo cáo toán học: " Dilemmas in the reliable estimation of the in-vitro cell viability in magnetic nanoparticle engineering: which tests and what protocols?" doc

Ngày tải lên : 20/06/2014, 20:20

... of factors including an increase in the coating diameter upon addition of the PEG moiety [33], thus increasing the stability of the nanoparticles and further shielding the cells from the iron ... either the presence of the MNPs both intracellular and on the membrane of the cells or the nanoparticles themselves interfere with the reagents In our experiments, wells with nanoparticles (intracellular ... [1] In the field of tissue engineering, magnetic nanoparticles have been previously reported for many applications including cellular labelling, sorting and monitoring, targeted in- vivo therapeutic...

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báo cáo hóa học:" Dilemmas in the reliable estimation of the in-vitro cell viability in magnetic nanoparticle engineering: which tests and what protocols?" potx

Ngày tải lên : 21/06/2014, 17:20

... of factors including an increase in the coating diameter upon addition of the PEG moiety [33], thus increasing the stability of the nanoparticles and further shielding the cells from the iron ... either the presence of the MNPs both intracellular and on the membrane of the cells or the nanoparticles themselves interfere with the reagents In our experiments, wells with nanoparticles (intracellular ... widely reported [35-38] In order to achieve a more in- depth understanding of the degree of cytotoxicity of the MNPs, we also investigated the effect the polymer-coated nanoparticles had on the integrity...

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Screening of Bacillus strains as potential probiotics and subsequent conﬁrmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs doc

Ngày tải lên : 07/07/2014, 19:20

... strains of spore-forming bacteria used as probiotics and then evaluate the strain of Bacillus showing the greatest in vitro potential for its effectiveness in improving piglet performance and ... exposure to chloroform vapor for 30 The chloroform was then evaporated for 20 0.1 ml of a 24 h culture of E coli K88 in Nutrient Broth (about 108 CFU/ml) was spread on the plate After 16–18 h incubation ... 634 and 744 were originally obtained from the fresh faeces of healthy piglets The size of the inhibitory zone for the six selected isolates against the four indicator pathogens is shown in Table...

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Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

Ngày tải lên : 19/02/2014, 12:20

... occurs at the active site or the glutamine-binding site of the enzyme (Sno1p) Although DON inhibition of Sno1p was not pursued further, it is worth pointing out that the cysteine serving as the key ... 000 g) for min, the absorbance of the supernatant was read at 363 nm The absorbance of the reduced form of APAD (APADH) was linear over the 2–100 lM range of glutamate with a molar extinction ... were correct at least to the 9th cycle from the N-terminus including the initiating Met: MHKTHSTMS for Sno1p and MTGEDFKIKS for Snz1p Properties of the complex of Sno1p and Snz1p When Sno1p with...

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CORREGGIO A COLLECTION OF FIFTEEN PICTURES AND A SUPPOSED PORTRAIT OF THE PAINTER, WITH INTRODUCTION AND INTERPRETATION pot

Ngày tải lên : 06/03/2014, 13:20

... whose writings and teachings shaped the doctrines of the faith in the early centuries of our era They interpreted for the people the meaning of the Scriptures and the Gospels The pendentive of our ... arm rests on the rim of a wheel, making a support for the hand holding the book The wheel is the emblem most frequently associated with St Catherine, as the reminder of the tortures inflicted by ... full of people on the same errand, and "there was no room for them in the inn." So it came about that the new-born babe was wrapped in swaddling clothes and laid in a manger used for feeding cattle...

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Ceylon; an Account of the Island Physical, Historical, and Topographical with Notices of Its Natural History, Antiquities and Productions, Volume doc

Ngày tải lên : 07/03/2014, 14:20

... Value of artificial irrigation in the north of Ceylon In the south of the island the rains sustain cultivation Two harvests in the year in the south of the island In the north, where rains are ... Gardner.] NORTHERN PROVINCES. Coral Formation. But the principal scene of the most recent formations is the extreme north of the island, with the adjoining peninsula of Jaffna Here the coral rocks ... farther to the west and south, so as to include Lanka or the equinoctial point of the Indian astronomers." _Discourse on the Institution of a Society for inquiring into the History, &c., of the...

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A Brief Memoir with Portions of the Diary, Letters, and Other Remains, potx

Ngày tải lên : 16/03/2014, 01:20

... Christ, and He would lead me safely through all The shining of the fire and the shading of the cloud are yet in the ordering of the Captain of Salvation 32 20th Exceeding poor; and yet I rejoice in ... discovery of these has had the effect of making me more distrustful of self And, now, oh that the everlasting covenant might be ordered in all things 13 and sure, and He only, who is King of Kings and ... in their behalf, in the world at large, is, in many cases, not according to knowledge An historical view of their points of contact with the professing Christian world, has long been on my mind;...

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sugar processing and by products of the sugar industry 144 potx

Ngày tải lên : 18/03/2014, 12:20

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PRODUCTS OF THE CHEMICAL OR ALLIED INDUSTRIES ppt

Ngày tải lên : 28/03/2014, 19:20

... synthetic organic colouring matter, synthetic organic products of a kind used as fluorescent brightening agents or as luminophores (heading 32.04) or dyes or other colouring matter put up in forms ... (Chapter 27); (c) The products of headings 29.36 to 29.39 or the sugar ethers, sugar acetals and sugar esters, and their salts, of heading 29.40, or the products of heading 29.41, whether or not chemically ... - -For use as a starting material in the manufacture of synthetic organic dyes or pigment dyestuffs; For use in the manufacture or filling of cylinders for calendering, supercalendering or embossing...

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Báo cáo khoa học: Analysis and biological relevance of advanced glycation end-products of DNA in eukaryotic cells ppt

Ngày tải lên : 30/03/2014, 04:20

... in Table The amount of CEdG formed during the DHA incubation of the vector pGL3 Control increased with both incubation time and quantity of glycating reagent The reaction mixture containing the ... Time (min) B Intensity (cps) were applied: (a) the appearance of a peak pair with retention times of 8.3 and 9.5 min, and (b) the presence and (c) the correct proportion of the three major mass ... times with NaCl ⁄ Pi, and stored frozen for further analysis The other half of the cells were incubated with 10 mL of reduced medium [MEM with Earle’s salts containing 1% fetal bovine serum and...

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Báo cáo khoa học: Spectroscopic and DNA-binding characterization of the isolated heme-bound basic helix–loop–helix-PAS-A domain of neuronal PAS protein 2 (NPAS2), a transcription activator protein associated with circadian rhythms docx

Ngày tải lên : 30/03/2014, 11:20

... NPAS2 by interacting with the bHLH region in a direct or indirect manner Therefore, it is worth studying how the bHLH domain contributes to heme binding to the PAS-A domain in NPAS2 and how the bound ... properties of the bHLH-PAS-A fragment We further examined the binding of CO to the Fe(II) bHLH-PAS-A domain (5 lm heme) of NPAS2, but the increase of the Soret absorption upon CO binding was composed of ... For the Fe(II) complex, on the other hand, m3 bands were observed at 1471 and 1493 cm)1, representing the ﬁve-coordinate high-spin and six-coordinate low-spin states, respectively Because of the...

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Báo cáo sinh học: " Imperfect DNA mirror repeats in the gag gene of HIV-1 (HXB2) identify key functional domains and coincide with protein structural elements in each of the mature proteins" doc

Ngày tải lên : 18/06/2014, 18:20

... aspects of gene and protein The interaction between DNA and protein structure and function is precise and interwoven over the entire length of the protein The distribution of mIMRs and rdIMRs and their ... on whether they are completely contained within another IMR The next L1 IMR ends downstream from the end of the preceding IMR; it may begin within a preceding IMR or downstream from it For the ... evaluating the symmetry of every possible sub-string of a DNA sequence, then nesting them sequentially, beginning at the 5' end The span of the first IMR is designated L1; all shorter IMRs within the...

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báo cáo hóa học: " The longitudinal link between visual acuity and health-related quality of life in patients with diabetic retinopathy" doc

Ngày tải lên : 18/06/2014, 19:20

... of the study protocol and the informed consent document The study was initiated at each site after the principal investigator and the study sponsor obtained approval documents The principal investigator ... investigator at each site was responsible for obtaining informed consent from each patient or legal representative and for obtaining the appropriate signatures on the informed consent document prior ... (ETDRS), and HRQL The scoring of all questionnaires followed the guidelines set forth by the instrument developers Categorical variables were summarized in terms of frequencies and percentages, and...

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FINANCIAL STATEMENTS AND SUPPLEMENTAL INFORMATION New York City Industrial Development Agency (A Component Unit of The City of New York) Years Ended June 30, 2010 and 2009 With Report of Independent Auditors _part1 potx

Ngày tải lên : 19/06/2014, 21:20

... compliance and the results of that testing, and not to provide an opinion on the internal control over financial reporting or on compliance That report is an integral part of an audit performed in accordance ... appropriate in the circumstances, but not for the purpose of expressing an opinion on the effectiveness of the Agency’s internal control over financial reporting Accordingly, we express no such opinion ... supplemental information IDA is considered a component unit of The City of New York (the City) for financial reporting purposes, and a public benefit agency of the State of New York (the State)...

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FINANCIAL STATEMENTS AND SUPPLEMENTAL INFORMATION New York City Industrial Development Agency (A Component Unit of The City of New York) Years Ended June 30, 2010 and 2009 With Report of Independent Auditors _part2 pptx

Ngày tải lên : 19/06/2014, 21:20

... the Financing Lease and related IDB financing Accordingly, with the exception of certain fees generated as a result of the Financing Transaction, the Financing Transaction is given no accounting ... Background and Organization The New York City Industrial Development Agency (IDA or the Agency) is considered a component unit of The City of New York (the City) for financial reporting purposes of the ... unit of The City of New York) Notes to Financial Statements (continued) Cash and Investments (continued) Investments As of June 30, 2010 and 2009, the Agency had the following investments Investments...

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FINANCIAL STATEMENTS AND SUPPLEMENTAL INFORMATION New York City Industrial Development Agency (A Component Unit of The City of New York) Years Ended June 30, 2010 and 2009 With Report of Independent Auditors _part3 doc

Ngày tải lên : 19/06/2014, 21:20

... either of Moody’s or S&P and (ii) the market value of the swap transactions covered by the credit support annex is in favor of the Agency in an amount that exceeds the threshold amount and the ... and Yankees in accordance with the terms of the lease agreements with the respective organizations The leases are considered direct financing leases for accounting purposes and, accordingly, construction ... defaults or if the swap is terminated The following table displays the terms of the Agency’s hedging derivative instruments outstanding at June 30, 2010, along with the credit rating of the associated...

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báo cáo hóa học:" The longitudinal link between visual acuity and health-related quality of life in patients with diabetic retinopathy pot

Ngày tải lên : 20/06/2014, 16:20

... of the study protocol and the informed consent document The study was initiated at each site after the principal investigator and the study sponsor obtained approval documents The principal investigator ... investigator at each site was responsible for obtaining informed consent from each patient or legal representative and for obtaining the appropriate signatures on the informed consent document prior ... (ETDRS), and HRQL The scoring of all questionnaires followed the guidelines set forth by the instrument developers Categorical variables were summarized in terms of frequencies and percentages, and...

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Báo cáo hóa học: " Research Article On the Connection between Kronecker and Hadamard Convolution Products of Matrices and Some Applications" pptx

Ngày tải lên : 22/06/2014, 02:20

... is of order mn × np, where fij t ∗ B t is the ijth submatrix of order n × p; thus A t A t ∗ B t is of order m × p, and similarly, the product A t •C t is of order m × n The following two theorems ... make some diﬀerent inequalities on using the commutativity of Hadamard convolution product Thus we have the following theorem I Theorem 3.1 For matrices A t and B t ∈ Mm,n and for s ∈ T T T T s ... , 3.5 and inequality 2.26 we can establish Theorem 3.1 8 Journal of Inequalities and Applications Further, Theorem 3.1 can be extended to the case of Hadamard convolution products which involves...

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