... not used except for demonstrating the program. The procedure that assigns pseudo-weight to orthography is roughly as outlined below, ignoring morphology, etymological and more special cases ... Ferrnra Ferrell Raherty Flanagan Fuchs Gallagher Gallo Galloway Garcia from Orthography 0.96 SRom 0,92 SRom, 0.08 1,00 SRom 0.95 Swed 0.47 MF 0.45 1.00 Ger 1.00 NBrit 1.00 N Brit ... input orthography and the output strew. The orthography stress problem will be split into two subproblems: • Orthography Weight • Weight ~ Stress 3. What is Sy~ Weight: Weight is a binary...
... Leno GH, Zlatanova J,de Grooth BG & Greve J (2001) Unfolding individualnucleosomes by stretching single chromatin fibers withoptical tweezers. Nat Struct Biol 8, 606–610.39 Rodriguez-Campos ... rapidly decreased suggesting the per-manent removal of B4 from the nucleosomes duringthe initial stretching. No correlation between the dis-ruption length and the energy barrier was found. ... arelaxed zigzag structure at low ionic strength andundergoes compaction with increasing salt concentra-tion, reaching a very compact form under physiologi-cal conditions. The linker DNA arrangement...
... M2-goa1-s, 5¢-CATTATAAGAACATAGGCGCTACAAGGATGGGTTGTACCATGTCACAGGAAG-3¢; M2-goa1-PstI-as, 5¢-CCAATGCATTGGTTCTGCAGTTAATACAA GCCGCATCCACGAAGA-3¢.(An engineered PstI recognition site is single-underlined.The ... thefollowing primers: M2-myc-EcoRI-s, 5¢-CAGAATTCatggagcagaagctgatctccgagga ggacctg ctg GTGAACAACTCCACCAACTCCTCCAACAACTCCCTGGCTCTTACAAGTCCTTATAAGACA-3¢; HsM2-as, 5¢-TTACCTTGTAGCGCCTATGTTCTTATAATG-3¢. ... of GPCRs derived from evolutio-narily distant organisms for the manipulation of G protein signalling. GPCRs recognize a wide varietyof ligands. Although some ligands are conserved inmany organisms...
... developed by Torr (1995) for long-range motion matching, in which geometricconstraints were used to remove outliers for rigid objects moving within an image.The ground-breaking work of Schmid and Mohr ... large change in relative magnitudes for some gradients, but are lesslikely to affect the gradient orientations. Therefore, we reduce the influence of large gradientmagnitudes by thresholding ... larger than0.2, and then renormalizing to unit length. This means that matching the magnitudes forlarge gradients is no longer as important, and that the distribution of orientations has greateremphasis....
... obtained from Biomol (Plymouth Meeting,PA), Sephadex G- 25 and G- 50 from Amersham Biosciences(GE Healthcare, Piscataway, NJ), Cleland’s reagent (dithio-threitol) from Calbiochem (San Diego, CA), ... aldehydes via binding of released zinc to pro-tein sulfhydryls is evident from the effects of releasedzinc on gene expression (Fig. 6) and phosphorylationsignaling (Fig. 7). Short-chain alcohols ... in control HepG2 cells is75.4 ± 7.6 ngÆ (g cells))1(Fig. 6). Treating the cellswith ethanol, a known inducer of MT [17], for 12 hincreases the concentration of MT to 101 ngÆ (g cells))1.To...
... openness and savings ratios in emerging markets (Regional percentage averages) Financial openness index 20071 Trade openness indicator (X+M)/GDP (average 2004-07) National savings rates as ... Highlighting Monetary Policy Challenges from a Negative Asset Price Bubble Perspective Andrew Filardo 355 November 2011 Anchoring countercyclical capital buffers: the role of credit aggregates ... Thailand. Finally, Emerging Europe is: Bulgaria, the Czech Republic, Estonia, Hungary, Latvia, Lithuania, Poland and Romania. Graph 1 Real credit: growth and cycle by regions1 Growth rates2...
... underlined)5¢-CTAGGTGAACATATGAGTGAGGAAAGAATTCC-3¢and the reverse primer 5¢-GGAGCTGGATTAATGCTCGAGTCTCCTATTAG-3¢ (the inserted XhoI restriction siteis underlined). The PCR products obtained were purified from ... Storz G & Zheng M (2000) Oxidative stress. In Bacter-ial Stress Responses (Storz, G & Hengge-Aronis, R,eds), pp. 47–59. ASM Press, Washington, DC.3 Touati D (2000) Iron and oxidative stress ... purified to homogeneity ina two-stage process using affinity chromatography onHisTrap HP and gel filtration on HiLoad Superdex 75obtaining 30 mg and 18 mg, respectively. TheSDS ⁄ PAGE of the final...
... IgEbinding to occur. Therefore, folding of more than oneduplex is not necessary to create an IgE bindingepitope. Given the repeated structure of Bla g 1, thedegree of IgE binding to Bla g ... by the German cockroach (Blattella germanica)is common [2–5]. Several German cockroach allergenshave been cloned including Bla g 1, Bla g 2, Bla g 4,Bla g 5 and Bla g 6 [6–10]. Bla g 1 is ... exposure to > 2 U g )1Bla g 1 isa strong risk factor for sensitization [5,15]. A recent reportsuggested that exposure to Bla g 1 or Bla g 2 at 3 monthsof age predicted allergen-specific...
... propargylglycine (PGG). Both GSH and Cys can be exported from cells into plasma, where theyundergo auto-oxidation. The liver and kidneys appear to have significant capacity for GSH efflux. Although ... ⁄ CySS or GSH ⁄ GSSG pool (e .g. 21and 9 mV changes, respectively, as observed in aging)is sufficient to cause a large increase in the oxidizedforms of intracellular proteins bearing vicinal ... efflux from a singleorgan because of pathophysiological conditions, and,more importantly, the influence of oxidative stress onthese processes. Our data clarify some of these aspects,and suggest...
... –IIB-IVB Glu113-Arg271 Glu116-Arg272 Glu112-Arg271IIB-IVB CO117-Arg276 CO120-Arg277 –IIA-IIB Asp39-Arg118 Asp96-Arg121 Asp92-Arg117IVB-IVC Arg276-Asp280 Arg277–281 Arg276-Asp2802560 A. Hofmann ... positivelycharged patch between domains III and IV. This figure was prepared withGRASP[36].Table 2. Conservation of salt bridges.Anx(Gh1) Anx24(Ca32) AnxA5IE-IIA Arg80-Glu99 – –IIB-IVB Glu113-Arg271 Glu116-Arg272 ... Tyr192IIIDE Lys230 Gly231IVAB Arg261 Arg262IVAB Arg262 Arg263Fig. 2. The membrane binding loops. The IAB loops of Anx(Gh1) (A) and AnxA5 (B) are shown in the same view from the front. (C) and (D)...
... primer: 5¢-GATGTTGATGGTGATGGTCA-3¢;reverse primer: 5¢-AAACCAGCCATGAATGAAAT-3¢)and with actin primers (forward primer: 5¢-GTTGGGATGAACCAGAAGGA-3¢; reverse primer: 5¢-GAACCACCGATCCAGACACT-3¢) ... oligonucleotides used for theseexperiments are: Oligo I, 5¢-CAAGGACGTTCGATGCACTTCCAAAAAACATATAAT-3¢; Oligo II, 5¢-CAATGTAGTATTAAAAAGTAGTAGTTAAAAGC-3¢; OligoIII, 5¢-GTTTTTATCCGATGCAAATTTTTGCTTTGTGATTG-3¢.The ... multiplestresses and probably has a role at the very beginning ofmultiple stress signaling pathways [37]. Involvementof CDPKs in stress signaling has also been shown byusing chimeric gene constructs...
... rapidly growing and aging population and will address the key risk factors. The government will play a vital role in improving the health system along the following levers: Promoting and encouraging ... improving their recruitment and training, enhancing the management of their performance, improving their image in society, and increasing the attractiveness of careers in teaching16 | From Regional ... a national competitive advantage as emerging global centres of low-cost manufacturing are gradually eroding this edge. We now need to rethink our place in the global value chain and identify...