g2 m phase arrest in glioma cells exposed to bo 1051
Báo cáo khoa học: " Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response" potx
... in days for tumors to reach 10 times the initial volume in mice treated with the combination of BO- 1051 and radiation minus the time in days for the tumors to reach 10 times the initial volume ... significant synergistic interaction in all three glioma cells Induction of a G2/ M phase arrest in glioma cells exposed to BO- 1051 Given that radiosensitivity is distinct in different phases of the cell ... considered to be statistically significant Results Determination of the cytotoxicity of BO- 1051 on different human glioma cell lines To determine the effects of BO- 1051 on glioma cell cytotoxicity...
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báo cáo hóa học:" Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles" doc
... was cultured in RPMI 1640 medium (PAA, Pasching, Austria), supplemented with 100 U of penicillin G/ml (Gibco, BRL, Eggenstein, Germany), 100 μg of streptomycin/ml (Gibco), mM L-glutamine Page of ... Huebner AK, Schinke T, Priemel M, Schilling S, Schilling AF, Emeson RB, Rueger JM, Amling M: Calcitonin deficiency in mice progressively results in high bone turnover J Bone Miner Res 2006, 21:1924-1934 ... Rueger JM, Gagel RF, Emeson RB, Amling M: Decreased bone formation and osteopenia in mice lacking alpha-calcitonin gene-related peptide J Bone Miner Res 2004, 19:2049-2056 25 Akopian A, Demulder...
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báo cáo hóa học:" Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles" pot
... was cultured in RPMI 1640 medium (PAA, Pasching, Austria), supplemented with 100 U of penicillin G/ml (Gibco, BRL, Eggenstein, Germany), 100 μg of streptomycin/ml (Gibco), mM L-glutamine Page of ... Huebner AK, Schinke T, Priemel M, Schilling S, Schilling AF, Emeson RB, Rueger JM, Amling M: Calcitonin deficiency in mice progressively results in high bone turnover J Bone Miner Res 2006, 21:1924-1934 ... Rueger JM, Gagel RF, Emeson RB, Amling M: Decreased bone formation and osteopenia in mice lacking alpha-calcitonin gene-related peptide J Bone Miner Res 2004, 19:2049-2056 25 Akopian A, Demulder...
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Báo cáo khoa học: "Increased betulinic acid induced cytotoxicity and radiosensitivity in glioma cells under hypoxic conditions" pptx
... example, BA is cytotoxic in different tumor cell lines, including neuroectodermal tumors, melanoma, colon, lung and ovarian carcinoma, head and neck cancers and sarcoma [2-4] Experiments in animals ... the mouse melanoma cell line B16F10, BA improved vincristine-induced cytotoxic effects in vitro, in addition to reducing the number of metastases in vivo [7] Sublines of the colon cancer cell line ... from - 30 M The half maximal inhibitory concentration (IC50) with an incubation time of 24 h was 24 M in U343MG cells and 23 M in U251MG cells Data represent mean values (± SD) of three independent...
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báo cáo khoa học: "Adenovirus-mediated delivery of bFGF small interfering RNA reduces STAT3 phosphorylation and induces the depolarization of mitochondria and apoptosis in glioma cells U251" pdf
... line U251 was cultured in Dulbcco’s modified Eagle medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (FBS), 100 U/ml of penicillin, and 100 μg/ml of streptomycin in a humidified ... production of the samples 2.5 Measurement of mitochondrial transmembrane potential (Δ m) Mitochondrial transmembrane potential (Δ m) was measured with the mitochondrial membrane potential assay ... humidified atmosphere containing 5% CO2 at 37°C All media and serum were purchased from Gibcol Normal human astrocytes (NHA) were obtained and maintained in specific growth medium AGM bullet kit from Clonetics-BioWhittaker...
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molecular merchanisms underlying inhibitory effect of fuciodan on cytokine-induced inflammation in glioma cells
... Molecular Mechanisms underlying Inhibitory Effect of Fucoidan on Cytokine-Induced Inflammation in Glioma Cells 2008 DO THI THU HANG Molecular Mechanisms nderlying Inhibitory Effect ... culture Rat glioma cell line C6, human blastoma cell line SH-SY5Y, human blastoma cell line SK-N-SH, human glioblastoma astrocytoma cell line U373-MG, and mouse macrophage cell line Raw264.7 ... Pathological mechanism of Alzheimer’s disease (J Clin Invest 2004 July 1; 114(1): 23–27) 1.4 Inflammation in Alzheimer’s disease Inflammation in AD is regulated mainly by microglia and astrocytes Microglia...
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Báo cáo khoa học: "Molecular analysis of hprt mutation in B6C3F1 mice exposed to ozone alone and combined treatment of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate for 32 and 52 weeks" ppsx
... primary culture, cells were then enumerated using a haemocytometer and cultured in 96-well U-bottom microtiter plates with supplemented medium to determine the clonal efficiency (CE) and to identify ... washing the recovered Molecular analysis of hprt mutation cells with RPMI 1640 medium The cells were then resuspended in primary culture medium for mitogenic stimulation for 36-40 hours Both primary ... 407-415 23 Masumura K, Horiguchi M, Nishikawa A, Umemura T, Kanki K, Kanke Y, Nohmi T Low dose genotoxicity of 2amino-3,8-dimethylimidazo [4,5-f] quinoxaline(MeIQx) in gpt delta transgenic mice Mutat...
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Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf
... sodium bicarbonate, 1% penicillin and streptomycin, 1.7 mM L-glutamine, 0.1 mM β-mercaptoethanol, ng/ml mouse leukemia inhibitory factor and 15% FBS The cells were grown on either gelatinized ... (Suppl 1), 43-58 Bonvin C, Guillon A, van Bemmelen MX, Gerwins P, Johnson GL, Widmann C Role of the amino-terminal domains of MEKKs in the activation of NF kappa B and MAPK pathways and in the regulation ... uptake is mediated by cAMP, protein kinase C, oxidative stress and mitogen-activated protein kinases in mouse embryonic stem cells Clin Exp Pharmacol Physiol 2006, 33, 211-220 32 Hausburg MA, Dekrey...
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Báo cáo khoa học: " Induction of oxidative stress and lipid peroxidation in rats chronically exposed to cypermethrin through dermal application" pps
... associated cardiolipin loss and membrane depolarization in rat brain mitochondria Neurochem Int 2006, 49, 20-27 Singh SN, Vats P, Kumria MM, Ranganathan S, Shyam R, Arora MP, Jain CL, Sridharan ... action on ciliary calcium channels in Paramecium tetraurelia Pestic Biochem Physiol 1999, 65, 181-193 Taplin D, Meinking TL Pyrethrins and pyrethroids in dermatology Arch Dermatol 1990, 126, 213-221 ... Turrens JF, Chang LY, Bush KM, Crapo JD, Freeman BA Detection of catalase in rat heart mitochondria J Biol Chem 1991, 266, 22028-22034 Rayman MP The importance of selenium to human health Lancet 2000,...
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Enhancement of tolerance development to morphine in rats prenatally exposed to morphine, methadone, and buprenorphine docx
... half-life is similar to morphine (70-90 minutes) in rats [21] In this test, the methadone injection protocol was similar to the morphine protocol as described above To measure antinociceptive effects ... tolerance to morphine in prenatally methadone- and morphine -exposed offspring The morphine-induced tolerance in prenatally methadone -exposed animals might also be due to opioid receptor expression ... response to morphine According to this finding, Robinson et al noted that buprenorphine seemed to have a greater ability than methadone to induce tolerance to morphine and did so in a dose-related manner...
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Báo cáo y học: " A single dose of DNA vaccine based on conserved H5N1 subtype proteins provides protection against lethal H5N1 challenge in mice pre-exposed to H1N1 influenza virus" ppt
... even in the unimmunized control mice Injection with DNA vaccines significantly increased the specific Abs in mice pre -exposed to H1N1 virus Cell-mediated immunity Cellular immune responses to DNA ... concern about safety of DNA vaccines always exists, including potential integration of plasmid into host genome, induction of autoimmune responses or immunologic tolerance, and so on, but DNA vaccines ... volume induced total respiratory infection that caused virus shedding from the lung and led to death from viral pneumonia - 10 days later [41] Immunization Mice were immunized with NP DNA, M1 ...
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Báo cáo y học: " Effect of recombinant IL-10 on cultured fetal rat alveolar type II cells exposed to 65%-hyperoxia" pps
... anti-inflammatory cytokines is shifted by a combination of an increase of pro-inflammatory cytokine and a stronger decrease of an anti-inflammatory cytokine rather than by increase in pro-inflammatory ... most important chemotactic factor during the acute phase of lung inflammation [17,18] In contrast, IL-10 is an anti-inflammatory cytokine that regulates the production of pro-inflammatory cytokines ... Rhandhawa I, Hanley JM, Ramanathan R, deLemos RA, Minoo P: The effects of IL-10 on proinflammatory cytokine expression (IL-1beta and IL-8) in hyaline membrane disease (HMD) Clin Immunol Immunopathol 1998,...
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Báo cáo y học: "Isoniazid prophylaxis differently modulates T-cell responses to RD1-epitopes in contacts recently exposed to Mycobacterium tuberculosis: a pilot study" pdf
... up in the responders* Group of subjects analysed Number of subjects N (%) Time months Time 1–2 months Time months Time vs time Time vs time IFN-gamma (IU/ml) No INH PHA PPD QTF-G RD1 proteins ... up in the responders* Group of subjects analysed Number of subjects N (%) Time months Time 1–2 months Time months Time vs time Time vs time IFN-gamma (IU/ml) No INH PHA PPD QTF-G RD1 proteins ... of a gamma interferon whole-blood enzyme-linked immunosorbent assay and an enzyme-linked immunospot assay Clin Diagn Lab Immunol 2005, 12:1311-16 Goletti D, Carrara S, Vincenti D, Saltini C,...
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Báo cáo y học: "Modulation of the transcription regulatory program in yeast cells committed to sporulation" ppt
... http://genomebiology.com/2006/7/3/R20 Time in SPM (h) Time in YPA (min) Time in SPM (h) Time in SPM (h) Time in YPA (min) Insulated (24 genes) Time in YPD (min) Repressed (245 genes) Transfer to YPA Time ... also in our experiment (a) Transfer Time (h) 0.5 (c) 910 20 40 25 genes Time in YPD (min) Time in SPM (h) Time in SPM (h) Time in YPD (min) 29 genes (d) Time in SPM (h) Time in SPM (h) Time in ... difficult to use this fraction as a quantitative measure for commitment reviews Defining the timing of commitment DAPI staining comment Cells (2 ml) were centrifuged for 30 seconds, fixed in 0.25 mM...
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báo cáo khoa học: "Osthole induces G2/M arrest and apoptosis in lung cancer A549 cells by modulating PI3K/Akt pathway" pptx
... expression might be the molecular mechanism through which Osthole induced G2/ M arrest Apoptosis, an important regulator in developmental processes, maintenance of homeostasis and elimination of the damaged ... Tong L, Vishwanath S, Rajaram M, Tridandapani S, Anant S, Kuppusamy P: Curcumin induces G2/ M arrest and apoptosis in cisplatin-resistant human ovarian cancer cells by modulating Akt and p38 MAPK ... analyzed by flow cytometry (B) The percentage of cells in G2/ M phase in histograms *p < 0.01, **p < 0.001 versus control group Osthole induces G 2 /M arrest To determine whether Osthole inhibits the...
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Báo cáo y học: "Cell cycle G2/M arrest through an S phase-dependent mechanism by HIV-1 viral protein R." pps
... Bourgain F, de Gramont A, Gendron MC, SobczakThepot J: Flow cytometry to sort mammalian cells in cytokinesis Cytometry A 2007, 71:1-7 51 Naruyama H, Shimada M, Niida H, Zineldeen DH, Hashimoto ... 288:1425-1429 21 Molinari M, Mercurio C, Dominguez J, Goubin F, Draetta GF: Human Cdc25 A inactivation in response to S phase inhibition and its role in preventing premature mitosis EMBO Rep 2000, ... Cdt1 to promote the loading of minichromosome maintenance (MCM) complex for initiation of DNA replication [58] In fact, Cdt1 and Cdc6 are mutually dependent upon each other for MCM loading and initiation...
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Báo cáo y học: "Cell Cycle Arrest by a Natural Product via G2/M Checkpoint"
... In addition, genistein is found to induce G2/ M cell cycle arrest in MCF-7 cells [2] Whereas anti-carcinogenic compounds like lunasin and lectins in soybean were shown to induce apoptosis in malignant ... G2/ M population increased from 11.6 % to 20.6 % compared to control whereas cells with 15 % CKBM treatment, the G2/ M population increased to 50.5 % The increase of cell population at the G2/ M phase ... also involved in the induction of G2/ M cell cycle arrest and inhibition in cell proliferation still need further studies In summary, we have used flow cytometry and Western blot analysis to examine...
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Báo cáo Y học: Early growth response-1 gene (Egr-1 ) promoter induction by ionizing radiation in U87 malignant glioma cells in vitro pot
... control medium for up to h, indicating a longer lasting secretion of activator molecules or growth factors Adding suramin, a potent inhibitor of growth factor receptor±ligand binding, to the culture ... wortmannin allows us to investigate downstream signalling of the PtdIns3 pathway and dependent activation events Suramin Suramin (Sigma, St Louis, MO, USA) was added as an aqueous 30 mM stock to ... activity was measured by employing a 340 R G Meyer et al (Eur J Biochem 269) commercial kit (Invitrogen) Luciferase activity was determined with a kit from Promega and measured in a luminometer (Berthold);...
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báo cáo hóa học:" Drugs targeting the mitochondrial pore act as citotoxic and cytostatic agents in temozolomide-resistant glioma cells" pptx
... from a WHO grade IV human GBM [34]), were maintained in standard culture conditions (37°C, 95% humidity, 5% CO2) in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), mM L-glutamine, ... permeabilization or inner membrane permeabilization (IMP) IMP produces the so called mitochondrial permeability transition (MPT) that compromises the normal integrity of the mitochondrial inner membrane which ... ADF cells in 96 well plates The following day cells were stained for 10 minutes in medium containing JC-1 at the final concentration of 50 μg/ml After removal of JC-1, a new medium containing...
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Báo cáo sinh học: " G0/G1 arrest and apoptosis induced by SARS-CoV 3b protein in transfected cells" pot
... http://www.virologyj.com/content/2/1/66 70 Cells in each phase (%) 60 50 40 30 20 10 SubG1 G0/G1 S G 2M 24h SubG1 G0/G1 S G 2M SubG1 G0/G1 S G 2M 36h SubG1 G0/G1 S G 2M 48h 60h SubG1 G0/G1 S G 2M 72h positive cells ... that expression of 3b protein blocked or delayed the progression of cells from G0/G1 phase into S phase, and induced cells toward apoptosis Next, using similar method, we investigated the effects ... Kim IK, Oh SH: Activation of calcium signaling by hepatitis B virus-X protein in liver cells Exp Mol Med 2003, 35:301-309 Nakamichi K, Matsumoto Y, Otsuka H: Bovine herpesvirus U(S) ORF8 protein...
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