fx fa and fb clusters of photosystem i
Báo cáo khoa học: Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum PSI-J is required for efficient electron transfer and stable accumulation of photosystem I pot
... characteristics similar to a PSI-F-deficient strain [20] It was concluded that, in 70% of the PSI complexes lacking PSI-J, the N-terminal domain of PSI-F is unable to provide an efficient binding site ... integrity and stabilization of a luminal domain involving at least PSI-N and the N-terminal part of PSI-F which is required for efficient electron transfer Fig Alignment of PSI-J sequences representing ... (Glu) and H (His)] are indicated with green arrows Note that the histidine residue is only conserved in cyanobacteria, in agreement with the notion that PSI-J of cyanobacteria is involved in binding...
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Báo cáo lâm nghiệp: "Effects of light intensity on the growth and energy balance of photosystem II electron transport in Quercus alba " potx
... light is severely limited Leaf weight ratio, specific leaf area, and leaf area ratio increased with the degree of shading, indicating acclimation of leaves to different light intensities Using ... fluorescence Light acclimation of light-saturated NPQ in Quercus alba seedlings is consistent with findings in Digitalis [26] and Hibiscus [44] The level of total NPQ at saturating PPFD of 725 μmol ... proportion of seedlings initiating the third growth flush did reflect light environment, with 0, 0, 20 and 71% seedlings initiating the third growth flush for 5, 15, 30 and 100% of full sunlight,...
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Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: photosynthetic electron transfer from photosystem I to NADP+ doc
... activity of Fld derives from its FMN cofactor The Fld semiquinone is exceptionally stable and its midpoint potentials are quite negative This is a direct consequence of the differing stability of ... medium and the AnFNR isoalloxazine N5 via S80 [58,62] In addition, in both enzymes, this residue is critical for proper binding of the nicotinamide to the active centre, CTC stabilization and ... Clostridium beijerinckii flavodoxin: evaluation of additivity and the importance of interactions provided by the main chain in the modulation of the oxidation-reduction potentials Biochemistry 40, 13548–13555...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... trypsin digestion on the cis-side of the membrane The A B 4004 Fig Determination of the topology of PSI-G in the thylakoid membrane using in vitro import experiments (A) Insertion of Arabidopsis ... 0, 30 and 60 digestion with trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D ... topology of PSI-G and to test the feasibility of rescuing Arabidopsis PSI complexes lacking PSI-G with tagged constructs Successful introduction of a hexa-histidine (His)- or Strep-tagged PSI-G into...
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Báo cáo khoa học: Photosynthetic acclimation: State transitions and adjustment of photosystem stoichiometry – functional relationships between short-term and long-term light quality acclimation in plants ppt
... photosynthetic organism As a result of various abiotic and biotic influences, light is highly variable in its intensity and its quality on both a shortterm timescale (in the range of seconds to minutes) and ... fluctuating intensities of white light or alternating illumination with PSI-light or PSII-light it showed retarded devel1084 opment in comparison to the wild-type plant [24,32] In addition, in field ... photosynthesis and enriched in far-red light (B) Preferential action of acclimation responses under the different illumination conditions Relative importance is indicated by the thickness of the symbol...
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Báo cáo khoa học: Protective effect of active oxygen scavengers on protein degradation and photochemical function in photosystem I submembrane fractions during light stress pdf
... Physiol 35, 15–44 Aro EM, Virgin I & Andersson B (1993) Photoinhibition of photosystem II Inactivation, protein damage and turnover Biochim Biophys Acta 1143, 113–134 Prasil O, Adir N & Ohad I ... occurrence of and properties of two different types of photoinactivation Plant Cell Physiol 11, 29–38 Satoh K & Fork DC (1982) Photoinhibition of reaction centers of photosystem I and II in intact ... electron carriers located on the acceptor side of PSI (A1, FX, FA, and FB) were damaged during the photoinhibition of PSI The loss of PSI activity in thylakoids isolated from spinach leaves exposed...
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Báo cáo khoa học: High thermal and chemical stability of Thermus thermophilus seven-iron ferredoxin Linear clusters form at high pH on polypeptide unfolding doc
... protein stability at high temperatures and extreme conditions is relevant for a variety of biochemical and biotechnological applications Intriguingly, the stability of a protein can be increased ... functional importance [16] We here report a detailed biophysical characterization of the stability of FdTt to thermal and chemical perturbation in solution in vitro Interestingly, the cubic clusters in ... which is believed to result in high stability It appears that most of the stabilizing features of FdTt are found in the vicinity of the [3Fe-4S] cluster, which is the cluster that has functional...
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Báo cáo Y học: Supermolecular organization of photosystem II and its associated light-harvesting antenna in Arabidopsis thaliana docx
... projections of single PSII particles) indicated the presence of large numbers of PSII–LHCII supercomplexes with attached M-type LHCII trimers M AT E R I A L S A N D M E T H O D S Isolation of inside-out ... the new types of supercomplexes At the site of the tentative CP24 subunit, the Fig Comparison of positions of M-type trimers in various types of Arabidopis and spinach PSII–LHCII supercomplexes ... organization of photosystem II (Eur J Biochem 268) 6025 Fig Final result of image analysis of twodimensional crystalline and comparison (A) Sum of 450 aligned crystal fragments from Arabidopsis;...
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Báo cáo khoa học: Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley pot
... characterize the composition of this minimal PSII identified in viridis zb63, thylakoids isolated from the mutant were analysed by SDS ⁄ PAGE and immunoblotting with antibodies specific for PSI and PSII ... PSII RC was already at a minimal level and did not change significantly even at high light intensity The inability of the viridis zb63 PSII mutant to adjust its antenna size to different light intensities ... that the minimal LHCII–PSII supercomplex binds about 100 Chls per PSII RC The binding of the additional LHCII trimers to this minimal PSII unit provides an outer peripheral system that increases...
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Báo cáo khoa học: Increased sensitivity of glycogen synthesis to phosphorylase-a and impaired expression of the glycogen-targeting protein R6 in hepatocytes from insulin-resistant Zucker fa ⁄ fa rats pptx
... culture of hepatocytes with leptin and insulin (Fig 8A) in agreement with previous findings [38] R6 protein was increased by 75% after combined culture with leptin and insulin (Fig 8B) Discussion ... rat liver in vivo is insulin-dependent It declines during insulin deficiency and is restored by insulin treatment [22,40] Another glycogen-targeting protein expressed in rat liver and designated ... 0.005 relative to no additions 1.6 R6 IR (AU) * 1.2 0.8 0.4 * 0.0 Fa/ ? fa/ fa Fig Expression of glycogen-targeting proteins in fa ⁄ fa and Fa ⁄ ? hepatocytes.Immunoreactivity to GL, PTG ⁄ R5 and R6...
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Báo cáo khóa học: Protein assembly of photosystem II and accumulation of subcomplexes in the absence of low molecular mass subunits PsbL and PsbJ pdf
... are circled Positions of PSI, Cyt b6f dimers and various LHCII subassemblies are circled in the silver-stained gel of the DpsbF mutant lacking all PSII complexes and were identified by MALDI-TOF ... thylakoids (data not shown) It did confirm the association of PsbO with PSII–LHCII supercomplexes in the wild-type and with the PSII core dimers in DpsbJ, as well as the absence of PSII–LHCII supercomplexes ... dimerization, and thus the role of PsbL in the dimerization process itself may be indirect The exact mechanism of PSII dimerization is not known but it is conceivable that several small PSII subunits...
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Báo cáo khoa học: "Photochemical efficiency of photosystem II in rapidly dehydrating leaves of 11 temperate and tropical tree species differing in their tolerance to drought" doc
... to dehydration were similar in all species No significant interspecific differences could be detected in the sensitivity of PS II maximal photochemical efficiency (F Some important differ) m /F ... clarified In particular, survival and growth of trees under conditions of low water availability imply optimization of water use through stomatal regulation, high photosynthetic efficiency during ... of high values of PS II maximal photochemical efficiency ) m /F v (F up to very strong levels of dehydration, and decline in photosynthetic activity, as estimated by PS II photochemical efficiency...
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Báo cáo sinh học: "A comparison between the β-globin gene clusters of domestic sheep (Ovis aries) and Sardinian mouflon" docx
... polyphyletic origin of the domestic sheep In fact, by means of this technique, A and B haplotypes can be easily distinguished since they differ in both number and length of restriction fragments and ... embryonic one Corsico-Sardinian islands (Neolithic) In wild mouflons (Ovis gmelini rnusimon) captured in Sardinia, two alleles at the adult /3-globin locus have been observed: HBB and HBB’u, with ... HI, and Eco RI and probed with plasmid pG16Ec3Bm2 (containing the 5’ of the goat E gene) and plasmid pGq5’ (containing the 5’ of the goat V -globin I !3F-globin gene) According to the hybridization...
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Báo cáo y học: "Eukaryotic large nucleo-cytoplasmic DNA viruses: Clusters of orthologous genes and reconstruction of viral genome evolution" pps
... Phycodnaviridae Poxviridae Asfarviridae Asco- and Iridoviridae Ascoviridae Iridoviridae Mimiviridae Marseillevirus Green algae; algal symbionts of paramecia and hydras Animals: insects, reptiles, birds, ... encoding a distinct enzyme of the phospholipase D family which is part of the virus envelope [41] and is involved in the formation of virus-specific vesicles in infected cells [42] It seems plausible ... In particular, the inclusion of representatives of two additional virus families, the Ascoviridae and the putative new family represented by the Marseillevirus, did not result in an erosion of...
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Báo cáo sinh học: " Exact p-value calculation for heterotypic clusters of regulatory motifs and its application in computational annotation of cis-regulatory modules" doc
... follows: ⎧ li if li ≤ ki , (9) [Λ(k1 , ,ks )(l1 , , ls ) ]i = i = ⎨ ⎩ ki if li > ki Definition A text Ti belongs to class Ci (λ1, , λs; q), ≤ i ≤ ki iff Length of Ti equals i, is the probability P ... data published in [34] on regulatory clusters in D melanogaster This compilation includes information on (i) known binding motifs for transcription factors, (ii) known CRM regions, and (iii) known ... interpretation of ChIP-chip experiments [5] In addition, our algorithm AHOPRO can easily be extended to amino acid sequences and applied in identification of protein domain signatures Additional file Tree...
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Báo cáo sinh học: "WordCluster: detecting clusters of DNA words and genomic elements" docx
... or supplies an anonymous sequence The minimum output consists of the basic statistics of the clusters (base composition, entity composition and statistical significance) and the statistics by ... means of the negative binomial distribution A p-value threshold is then used to filter out those clusters which are not statistically significant A main difference to the originally described ... DA: Distance preferences in the arrangement of binding motifs and hierarchical levels in organization of transcription regulatory information Nucleic acids research 2003, 31:6016-6026 Sandelin...
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Synthesis and reactivity studies of cyclopentadienyl derivatives of ruthenium iridium and osmium iridium mixed metal clusters 5
... continuation of our reactivity studies on 3c, we have investigated its reactivity with chalcogenide substrates under mild conditions The results of these studies are discussed in the following ... cluster IrOs3 metal core with the iridium at one of the wingtips, and a telluride bridging each of the two Os-Os of the Os3 wing Besides the Cp*, the iridium also carries a carbonyl group Clusters ... distillation of solvents, preparation of starting materials and characterization techniques were identical to those described in the experimental section of Chapter Selective decoupling experiments...
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Synthesis and reactivity studies of cyclopentadienyl derivatives of ruthenium iridium and osmium iridium mixed metal clusters 1
... osmium compounds Notable results have been reported for a ruthenium:iridium and osmium:iridium ratio of 3:1 The tremendous potential for ruthenium-iridium and osmium-iridium clusters in catalytic ... pentamethylcyclopentadienyl ligand (Cp*) is a significant modification of Cp It is more bulky and more electron releasing than Cp The Cp* ligand can stabilize a wide range of complexes and it has been employed in ... ligand in transition metal carbonyl cluster chemistry owing to its versatility, range of bonding modes exhibited, property of stabilizing metals in low oxidation states and also its small size...
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Synthesis and reactivity studies of cyclopentadienyl derivatives of ruthenium iridium and osmium iridium mixed metal clusters 2
... chemistry and reactivity of Cp*Ru3Ir and Cp*Os3Ir clusters, attempts were made to synthesize Cp*- containing triosmium-iridium clusters The results obtained along with the characterization of ... [25] IR monitoring of the reaction indicated that is not formed directly in the reaction but has resulted from decomposition Addition of silica-gel to the crude reaction mixture and stirring for ... Ru2IrH3(CO)11(PPh3) I Ru4H2(CO)12(PPh3) Ru2IrH3(CO)11(PPh3) III Ru3IrH(CO)12(PPh3) II Scheme 2.3 28 The low temperature 1H NMR of cluster III suggested the presence of two isomers IIIa and IIIb...
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Synthesis and reactivity studies of cyclopentadienyl derivatives of ruthenium iridium and osmium iridium mixed metal clusters 3
... Reaction of 3a with MeCCBut Thermal reaction of 3a with 4, 4-dimethyl 2-pentyne afforded red crystals of 10c, with IR spectroscopic characteristics similar to those of 10a and 10b The identity of ... Schematic diagram of 16 97 3.4.3 Role of amine The most intriguing aspect of this study, however, was the mechanistic role that the amine played in these reactions Monitoring the reaction of 4b with ... the coordinated “alkyne” in these clusters show considerable variations, ranging from 1.34 to 1.56 Å, indicating considerable bond lengthening and possible deactivation Following a criterion used...
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