functionality of extracellular enzymes substrates in marine environments
Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
... amino group of the main chain at Glu268 The oxygen of the main chain at Asp266 was bound to amino groups of main chain of Tyr269 and Ala270 by hydrogen bonds The interactions should maintain ... from those obtained by treating the enzymes with high alkalinity Several mutant enzymes showed drastically improved stability in solutions containing SDS (Fig 4) ALP I maintained 60% of enzymatic ... core structure of ALP I independently As the side chain of Asn266 would be able to bind to the main chain of Ile10 with a hydrogen bond by substitution of Asp266Asn, the mobility of the two regions...
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Báo cáo khoa học: Structure of amyloid b fragments in aqueous environments docx
... aggregates of the mutant proteins using thioflavine T (ThT)-binding analysis The results confirmed the role of the regions in fibril formation of Ab in aqueous solution Results Purification of recombinant ... formation of aggregates of the wildtype and variant proteins using ThT-binding analysis in order to determine whether the introduced amyloidogenic sequences induce amyloid formation in the host protein ... exchange mapping of Ab using NMR revealed that the middle of Ab is involved in a b structure in the fibrils [30] In contrast, the C-terminal region, including residues 28–42, is important in nucleation...
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Characterization of Spin Coated Polymers in Nano-environments as a Function of Film Thickness pot
... divided spin coating into the four stages of deposition, spin-up, spin-off, and evaporation (Figure 2.31) By taking both the force of spinning and the concentration-controlled evaporation into account, ... material by finding the inflection point in the Tg step 3.1.3.2 Gel Permeation Chromato graphy A 3.160 mg/mL solution of PMMA in chloroform was prepared One sample of 100 µL was injected into a Waters ... a crosslink in the polymer by restraining certain chains.11 For thin films, those less than approximately 60 nm, significant increases in Tg could occur These researchers saw shifts of as much...
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Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt
... displaying two putative splice variants [Fig 2] Expression of fucokinase cDNAs The two splice variants of fucokinase were subcloned into the XbaI site of a pQM vector containing a C-terminal E2-Tag/A ... variants of L-fucokinase The long splice variant of L-fucokinase consisted of 3270 bp, encoding a protein of 1090 amino acids The sequence of the shorter cDNA was similar to the sequence of the IMAGE ... alternative splicing maintains the reading frame along the entire protein, therefore the protein variants are identical in the amino-terminal end up to the alternative splice area, in addition to...
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Báo cáo hóa học: " Research Article Automatic Detection and Recognition of Tonal Bird Sounds in Noisy Environments" pot
... multi-condition training approach, that is, the models were trained using a set of noisy training data The training and testing data were obtained as follows For each bird syllable, the detection of bird ... the information of interest in the signal Our aim is to investigate an employment of tonalbased features which are obtained using the spectral-level detection method presented in Section Since ... improved by using models trained on noise-corrupted training data, since such models can accommodate the effect of noise The use of the same noise conditions for training the models, and testing is generally...
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Báo cáo hóa học: " Research Article Characteristics of MIMO-OFDM Channels in Indoor Environments" pdf
... certain inconsistencies in the case of LoS environments, which point to the need for further model development The effects of varying antenna element spacing are also investigated, taking into ... spatial fading of a narrowband channel in indoor environments is often assumed to be Rayleigh distributed even in LoS link due to many scattering objects surrounding both Tx and Rx The degree of multipath ... characteristics of the channels in a number of indoor local areas We show that the channel can change as a function of antenna location in the order of a fraction of a wavelength in these multipath environments...
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Báo cáo y học: " Egr-1 inhibits the expression of extracellular matrix genes in chondrocytes by TNF-induced MEK/ERK signalling" ppsx
... localized to the extracellular space determined that many of the protein products of these genes were involved in a variety of activities, including chemokine/cytokine activity – including macrophage ... et al Figure Egr-1 in chondrocytes nallingDNA binding activity increased by TNF-induced MEK1/2 sigEgr-1 DNA binding activity isis increased by TNF-induced MEK1/2 signalling in chondrocytes Cells ... stimulating factor (Csf-1), were regulated in a MEK1/2-independent manner Surprisingly, the activation of NFB and the inhibition of Sox9 activity by TNF were independent of MEK1/2 The DNA binding...
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Recovery of antibiotic resistance genes in natural environments
... most plasmid had bands of fragments, which are indication of a right insert The remaining lanes showed only single bands these may due to the size of insert equals to the size of vector or the vector ... resistance Clin Microbiol Rev 14: 836-871 25 Benveniste, R., and Davies, J (1973) Aminoglycoside antibiotic-inactivating enzymes in actinomycetes similar to those present in clinical isolates of antibiotic-resistance ... canal in Ho Chi Minh city For screening antibiotic resistant E.coli strains bearing recombinant pUC19 plasmids, common antibiotics such as gentamicin, tetracycline, chloramphenicol, streptomycin...
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CHARACTERISTICS AND DISTRIBUTIONS OF NITROUS OXIDE-PRODUCING DENITRIFYING FUNGI IN NATURAL ENVIRONMENTS
... beginning and end of the experiment The contamination of bacteria or fungi was tested by the same procedures as the beginning of the incubation N2O analysis The N2O concentrations were determined ... was prepared in a vial of 70ml volume It contained a 3g (as dry wt ) of each sample pretreated as described above, 3ml of 0.1M phosphate buffer (in situ pH of each sample), containing potassium ... those in the soils Discussion The determination of contributions of individual microorganism to total metabolism in an ecosystem has remained difficult because of methodological problems The individual...
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Tài liệu Báo cáo khoa học: Reconstitution of coupled fumarate respiration in liposomes by incorporating the electron transport enzymes isolated from Wolinella succinogenes docx
... very low [19] In this paper, we address the following questions: (a) can coupled fumarate respiration be restored by incorporating the isolated enzymes into liposomes containing menaquinone; (b) ... The unit of activity (U) corresponds to the transfer of lmol electronsặmin)1 Protein was determined using the Biuret method with KCN [23] Measurement of H+/e ratios Proteoliposomes containing hydrogenase, ... derivatives of pHydcat [1] Plasmid pHydcat contains the entire hydABC operon and integrates into the genome of W succinogenes by homologous recombination Derivatives of pHydcat were synthesized using...
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Báo cáo khoa học: Molecular cloning and characterization of methylenedioxy bridge-forming enzymes involved in stylopine biosynthesis in Eschscholzia californica doc
... (S)-cheilanthifoline, not (S)-nandinine [7,30,31] If we assume that (S)-nandinine is produced in E californica, there may be no need for a catalyst from (S)-nandinine to (S)-stylopine However, nandinine could ... bridge-forming P450 (CYP719) cDNA, the protein of which catalyzed the conversion of (S)-tetrahydrocolumbamine to (S)-tetrahydroberberine [(S)-canadine] in berberine biosynthesis, from cultured cells of ... and the flow rate (0.5 mLÆmin)1) To determine the kinetic parameters of CYP719A2, the amount of nandinine produced was estimated by using a calibration curve of (S)-scoulerine (pmol versus peak area)...
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Báo cáo khoa học: Trigger factor interacts with the signal peptide of nascent Tat substrates but does not play a critical role in Tat-mediated export pptx
... Identification of a twin-arginine leader-binding protein Mol Microbiol 40, 323–331 Papish, A.L., Ladner, C.L & Turner, R.J (2003) The twinarginine leader-binding protein, DmsD, interacts with ... regulatory role in controlling the entry of secretory proteins in distinct targeting/translocation pathways [6,8] Photo-crosslinking experiments revealed contacts of the signal peptide of nascent ... production of full- Fig Photo-crosslinking of TF to the signal peptide of nascent SufI chains of various lengths In vitro translation of full-length SufIHATAG8 from non-truncated mRNA in the presence of...
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Báo cáo khoa học: "Corpus-based interpretation of instructions in virtual environments" pot
... and Regina Barzilay 2009 Reinforcement learning for mapping instructions to actions In Proceedings of the Joint Conference of the 47th Annual Meeting of the ACL and the 4th International Joint Conference ... Gargett, Konstantina Garoufi, Alexander Koller, and Kristina Striegnitz 2010 The GIVE-2 corpus of giving instructions in virtual environments In Proceedings of the 7th Conference on International ... them in an 80/20 proportion; the first 80% of data collected in each virtual world was used for training, while the remaining 20% was used for testing For each pair (uk , ck ) in the testing set,...
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Báo cáo khoa học: The role of Tyr71 in Streptomyces trypsin on the recognition mechanism of structural protein substrates ppt
... degradation of recalcitrant animal proteins, such as collagen [12,13], keratin [14], those involved in blood clotting [15,16] and amyloid prion proteins [17], has been of interest because of potential industrial ... equivalent in ninhydrin colour to 1.0 lmol of leucine in h at pH 7.4 at 37 °C in the presence of CaCl2 Data are expressed as the mean ± SD of three independent experiments (C) The ratio of hydrolytic ... findings using RIBS in vivo DNA shuffling to show that Tyr71 of SOT (corresponding to residue 89 of a-chymotrypsin numbering) contributes to the topological specificity This finding has not been inferred...
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Báo cáo khoa học: Role of extracellular signal regulated kinases 1 and 2 in neuronal survival docx
... role in neuronal protection in status epilepticus Inhibition of ERK1/2 activation with SL327 increased mortality in rats with pilocarpine-induced seizures [35] Ó FEBS 2004 Interestingly, in several ... depolarization in trophicdeprived neurons (reviewed in [2]) Importantly, as the studies indicating a lack of ERK1/2 involvement in antiapoptotic signaling used a variety of neuronal cells, the role of ERK1/2 ... Ca2+ in ux into isolated synaptosomes [8] The interpretation of the data obtained through experimental modulation of ERK1/2 signaling may be hampered by the fact that inhibition of one element of...
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the biology of human survival life and death in extreme environments sep 2003
... communication, or intercellular signaling For example, some of the members of an important family of cytokines, known as interleukins, regulate body temperature during infection In fact, the first of these ... to Bert, Bernard’s thinking influenced many of the great physiologists of the early half of the twentieth century, including Joseph Barcroft and J S Haldane in England Barcroft, who lived from ... something is missing in this way of thinking about physiology Living organisms change the actions they take in response to a change in the environment simply on the basis of the change For instance,...
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Báo cáo hóa học: " Electrical characterisation of deep level defects in Be-doped AlGaAs grown on (100) and (311)A GaAs substrates by MBE" docx
... constant for each trap are given in Table This study reveals that the number of traps, including some electron emitting deep levels, increases with increasing Be-doping for the samples grown on (100) ... (HEC), Pakistan for funding his PhD studies at University of Nottingham, UK Author details School of Physics and Astronomy, University of Nottingham, Nottingham NG7 2RD, UK 2Nottingham Nanotechnology ... http://www.nanoscalereslett.com/content/6/1/180 Page of compared to those grown on (100) substrates Our finding is a direct confirmation of their argument Experimental details A set of six AlGaAs samples with different Be-doping concentrations...
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báo cáo hóa học:" Landscape features and weather influence nest survival of a ground-nesting bird of conservation concern, the greater sage-grouse, in humanaltered environments" ppt
... also retained the date of initiation of the incubation process (Julian date) and the nest age in the model (Table 2) The final landscape model thus included seven covariates, including the intercept ... first day of incubation Thus, we used this date as the initiation date of incubation We projected the expected hatch date using the average incubation period of 27 days from the first day of incubation ... nest was initiated Similarly, the age of the nest (number of days since incubation began) was modeled to examine whether nests early or late in incubation had a greater probability of surviving Before...
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Báo cáo hóa học: " Research Article Recognition of Faces in Unconstrained Environments: A Comparative Study" pptx
... research in this direction has been reported in [10]) A last interesting aspect to be mentioned is that the proposed strategy of using a reference set of images in the case of comparing pairs of images ... the effect of using different region sizes in the performance of the analyzed methods Note that increasing the size of the regions corresponds to adding or removing different amounts of background ... concept of congealing Congealing basically consists of searching a sequence of transformations (in this case affine transforms and translations) that are applied to a set of images in order to minimize...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008