functional analysis of the cervicalfunctional analysis of intergenic
Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc
... eggs in the one-cell stage to visualize the localization and to mea-sure the mobility of the components in the PGCs. The medaka embryo was peeled off the chorion, and the segment containing the ... on the same setup of the laser scanning microscope as used for FCS analysis. The detection gain was adjusted to the fluorescence of the GFP fusion proteins almost at the saturation level of the detector, ... in the HeLa cell [24], suggesting the existence of some interactive cellular partner.FRAP analysis of the nuage revealed the slow recovery of Olvas–GFP (Fig. 3B). When the whole part of the single...
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Situational analysis of the socioeconomic conditions of orphans and vulnerable children in seven districts in Botswana pot
... Morupule. The local mine Morupule Colliery supplies the coal for the power station. Most of the people living in Palapye are employed by either the power station or the colliery. The other groups of ... coping with the burden of orphans and providing support. The caregivers acknowledge that there are lots of OVC who are cared for by the government. As in Palapye, one of the strengths of the government ... take care of their own needs as well as the needs of the children with whom they live. The conditions under which OVC are raised indicate that most of them live in poverty. Indicators of poverty...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... envelope of the bound protein. The percentage of protein bound to RNA was the ratio between the value of bound protein and the total protein present in the sample. The relative percentage of binding ... evaluation of the available modelon the interaction and cleavage of the RNA substrateand the Kid toxin is of interest in itself because it is the basis of important cellular roles of this toxin ... test the formation of astable heterooctameric Kid2–Kis2–Kid2–Kis2complexon the parD promoter [9]. We further analysed the effects of the mutations on the co-regulatory activity of the...
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analysis of the final scenes of alfred hitchcock
... shiftingpoints of view, andlight and dark to help explain the relationships between Alicia,Devlin,Sebastian and Mrs. Sebastian, and an overall theme of beingtrapped. An analysis of the film from the ... scene to the finalscene in the film shows how the above tools lead to a better understanding of the character's motivations. The most obvious recurring object in the final scenes is the poisonedcoffee ... pours the coffee into the cup for Alicia, and sets it on a small table infront of her.Here, Hitchcock not only zooms in on the small teacup, butheightens the soundit makes connecting to the...
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Báo cáo khoa học: In silico analysis of the adenylation domains of the freestanding enzymes belonging to the eucaryotic nonribosomal peptide synthetase-like family pot
... no. Q7QKF0) where the Asp235 ismissing. In this latter case, the absence of the Asp235can be explained in the light of the model of the inter-action of the substrate with the active site (vide ... such enzymes. The structural and functional roles of the residues involved in the substrateselection and binding are proposed through the analysis of the predictedinteractions of the model active ... moiety tethered to the phosphopantetheinylgroup and the free amino group of the downstreamaminoacyl moiety, thus facilitating the translocation of the growing chain onto the next module. The structural...
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Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides doc
... Likewise, these glycopro-teins are localized in lysosome, however, Man6-SO4accounts for the majority of the sulfated sugar.In conclusion, the results obtained provide evidence of the nature of the ... that of the untreated sample (Fig. 5A), assuring the high-mannosenature of the sulfated glycans.Table 1. m ⁄ z-Value, composition and structure of the high mannose and complex type glycans of ... UV-MALDI-TOF MS analysis of the oligosaccharides released from C-terminaldomain by PNGase F treatment in the linearnegative-ion mode using nor-harmane asmatrix. (A) Analysis of the total oligosaccha-ride...
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Fundamentals of Current Pension Funding and Accounting For Private Sector Pension Plans: An Analysis by the Pension Committee of the American Academy of Actuaries pptx
... associated with the passage of time during the year. This is offset against the other cost items and is generally the expected long-term rate of return on assets multiplied by the beginning of year ... percent of the greater of the plan’s PBO or MRVA. The total of the above pieces of the NPPC can produce a negative expense, which appears as income on a company’s financial statements. The accrued ... that the retirement benefit is based on the pay at retirement. To allow the plan sponsor to recognize the cost of the plan gradually over the participant’s lifetime, the actuary considers the...
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Báo cáo khoa học: Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA pot
... datanot shown). On the basis of these results, we concludethat the absence of either of these two clusters of basicamino acid within the linker does not affect the ability of the protein to preferentially ... tyrosine on the scissile phosphate in the DNA, resulting in the cova-lent attachment of the enzyme to the 3¢ end of the broken strand [2]. Rotation of the duplex regiondownstream of the break ... manifestation of the preferencefor supercoils. It is noteworthy that the elimination of either of the clusters of basic amino acids within the linker region (Fig. 1) does not affect the preference of the...
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Báo cáo khoa học: Structural adaptation to low temperatures ) analysis of the subunit interface of oligomeric psychrophilic enzymes pdf
... interpreted in the light of the work of Bahadur et al. [36]. These authors stud-ied the subunit interfaces of 122 homodimers, andshowed that the distribution of the area between the rim and core ... from those of theirmesophilic and thermophilic homologs. The most vari-able features are the increase in the number of ionicinteractions, the decrease in the number of hydrogenbonds, the decrease ... using a set of 512 points locatedon a sphere around each atom. The sphere interaction radius of each atom is equal to the sum of the van der Waals radius of the atom type plus the radius of a water...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... and then the energyminimization of the model was iterated 300 cycles using the conjugate gradient methods with the programDISCOVER_3inINSIGHT II. To construct the dimeric form of LPL, the model ... as the high-affinity peak of wild-type LPL. The LPL in the eluate wasdetected by Western blot with LPL polyclonal antibody7640. The fraction number of the high-affinity peak was the same for the ... mutant, the two LPL peaks both eluted at lower salt concentrationsthan those of wild-type LPL. The R405A mutant exhibitedan earlier elution of the high-affinity peak, whereas the elution of the...
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Báo cáo khoa học: Functional and genetic characterization of the promoter region of apolipoprotein H (b2-glycoprotein I) ppt
... eliminate the possibility of the functional roles of the )643T>C and )1219G>A SNPs as part of the extended APOH promoter transcriptional machinery.To further substantiate the functional ... Further studies will be needed to explore the mechanism for these associations.APOH promoter SNPs explain a small proportion of the variance in APOH expression. Therefore, the ability of these ... with the univariate data,none of the haplotypes defined by the minor alleles at the APOH promoter SNPs reached significance.Although the )32C>A SNP was significant in the univariate analysis, the...
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Báo cáo khoa học: Mapping of the interaction site of CP12 with glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii Functional consequences for glyceraldehyde-3-phosphate dehydrogenase pot
... with the A8B8isoform of GAPDH lead-ing to the A2B2isoform with a higher NADPH-dependent activity [39].By analogy with the CTE of the isoform of GAPDH[15,37], we hypothesize that the ... Sparla et al.on the A2B2isoform of GAPDH [24,37], formation of adisulfide bridge in the oxidized CTE prevents the inter-action between the 2¢-phosphate of NADPH and the side chain of Arg77 (spinach ... Wetherefore mutated Asp36 and Glu39 of CP12 into Alaor Lys. To test whether the interactions of thesemutants with the other partner, GAPDH were im-paired, we tried to reconstitute in vitro the...
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- one of the ultimate goals of teaching
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