... upstream ofthe STOP codon ofthe ORF of interest and on the 3¢ end, 65–70 nucleotides ofthe genomic region downstream ofthe ORF of interest Transformants were recovered based on their geneticine ... compare the sites of action of these polypeptides, C-terminal fusions of Sfh-proteins with yEGFP were constructed andthe localization ofthe proteins inthe yeast cell was determined The enzyme ... integrative versions of C-terminal yEGFP fusions in frame with the ORF of interest Thus, yEGFP fusions were present in single copy inthe genome and driven by the native promoter ofthe ORF of...
... p300–C(1571–2370) The intensity of binding, expressed as percentage of input bound, is indicated by different shades of gray and only interactions resulting in binding to more than 1.5% of input are shown ... given combination of proteins by the value obtained when the fold induction for each ofthe proteins present inthe combination were added (Table 1) There was little synergy inthe absence of cotransfected ... Thus, the bulk ofthe interaction between NF-jB and p300/CBP is mediated by the p65 subunit through the N- and C-terminal regions ofthe coactivators The pattern of IRF-1 binding to p300 and CBP...
... utilization of ICTs is examined, looking at NGO evolution, and NGOs and information, the Internet and Web 2.0 applications Finally, Internet use in Nepal andthe ideology of development inthe country and ... work of this type of Nepali NGO in terms ofthe degree of bottom-up development and social change it supports The study examines how these NGOs are communicating their work and advocating for their ... ICT use in Nepal is presented The research questions are then put forth andthe thesis‟ structure is outlined Discussions of ICTs and inequality, and development andthe Internet inthe global...
... the middle parts ofthe grove from points Sampling points St.1 and St.2 are inthe same stream, and sampling point St.3 is inthe other stream The two streams flow together to be one stream and ... and periphery ofthe Seto Inland Sea The location ofthe research area andthe distribution ofthe citrus groves in Matsuyama region are shown in Fig Matsuyama is situated in Shikoku Island in ... kind of citrus called Iyokan is mainly cultivated There are two small streams on theupper part ofthe hill andthe streams flow together from 47 m above sea level The water inthe stream is the...
... all the old University Oars now alive, requesting them to favour me with their experience ofthe after effects of training and boat-racing on their own health ; and I further begged them to inform ... emigrated, and were either residing in our Colonies or in other parts ofthe world, andthe only method I could discover of obtaining information regarding several of viil INTRODUCTION them, was ... captain ofthe John +, a boat which has often played a prominent part inthe struggles ofthe Isis, and which has served as the training-school for no fewer than ten ofthe Crews which during the...
... patterns of hydrophobins HFBIII of H jecorina and T longibrachiatum, and SRH1 and HFB1 of T atroviride, involving signal peptides, Kex2type processing, and C-terminal amino acid cleavage (Table 2) In ... spectra of H jecorina strain QM 9414 (D), andthe mutant strains QM 9414 Dhfb1 (B) and QM 9414 Dhfb1Dhfb2 (A) HFB I and HFB II indicate the processed hydrophobins Whereas the HFB I peak is missing in ... calculation of successive deletions of terminal amino acid residues of all six hydrophobins providing this single match A rapid means of identifying peaks corresponding to hydrophobins in MALDI-TOF mass...
... a This strain was grown in media lacking leucine The other strains were grown in media containing leucine Complementing abilities of LEU2, UTR1 and YEF1 for the growth defects ofthe pos5 mutant ... confirm the complementing ability of LEU2 for the growth defects ofthe pos5 mutant, we examined the growth of pos5 cells containing CgLEU2 instead of POS5 on the chromosome, ScLEU2 (LEU2 of S cerevisiae) ... mixture containing compounds at the indicated concentrations as described in Experimental procedures The effect of NADP and HgCl2 was examined by the stop method and others, by the continuous method...
... crab chitin-binding protein [15], as well as with the chitin-binding domain of hevein (Cys12 to Cys33), a plant chitin-binding protein [41,42], as shown in Fig This segment of tachycitin forms ... (Fig 6) In mammals, insulin-like growth factor-binding proteins, which contain the IGFBP motif, modulate the actions ofthe insulin-like growth factors in endocrine, paracrine, and autocrine systems ... Y9), and repeats ofthe di- and tri-peptide sequences QQ and QQQ were observed inthe amino terminal sequences of basic QH proteins and 10 Nucleotide sequences of HMM chitin-binding proteins cDNA...
... presence of saturating concentrations ofthe metal cofactor Together, these data are indicative of two modes of substrate binding (i.e open and closed) The closed, active form of UDP-Glc binding is ... concentrations, as the metal binding event would be totally independent ofthe interactions involved inthe enzyme–substrate complex The binding of MUG and Mg2+ are not independent, however, but instead ... involved inthe transferase Fig Diagram showing the thermodynamic equilibria andtheir dissociation constants involved inthe binding of UDP-Glc, MUG and Mg2+ to TcdA Values shown in parentheses...
... activity by interacting with the enzyme–substrate complex, denaturing the enzyme protein, interacting with its active sites (Nannipieri, 1994) or by affecting the synthesis ofthe enzymes within the ... complexation of Cd to polyphosphate granules inthe cell membrane and intracellular Cd binding proteins in a tolerant strain of Pseudomonas putida, grown in a defined medium containing mM Cd2q ... components (including the organisms) In addition, the total amount of bioavailable metal in soil is influenced by complexation with dissolved organic and inorganic ligands The toxicity of Cd to the mineralization...
... have the right to information about theirhealth care and what is going to happen to them (86.6%); children should be asked their views by theirhealth professionals (75%); hospitals andhealth ... must go to all the children who participated inthe consultation process by completing the survey and otherwise sharing their experiences, views andtheir perspectives about theirhealth care 22 ... consistency in this question with the same proportions – roughly four out of five children saying that they were given the information they wanted at their appointment with theirhealth care professional,...
... Moreover, the abundant pores on the surface ofthe R-Fe2O3 nanorods can facilitate the diffusion ofthe gas molecules and enable them to access all surfaces ofthe nanoparticles contained inthe sensing ... length ofthe nanorods increased with the increase inthe reaction time On the basis ofthe above results, a growth mechanism ofthe porous R-Fe2O3 nanorods can be proposed The schematic diagram of ... a porosity of type IV with a distinct hysteresis loop inthe range of 0.5-1.0 P/P0.36 The curve of pore size distribution ofthe porous R-Fe2O3 nanorods is shown inthe inset figure The curve exhibits...
... alpha index andthe gamma index The alpha index is defined as the ratio ofthe number of actual circuits to the maximum number of circuits A circuit is a finite, closed path starting and ending ... indicating distance to other homes/buildings, type of housing inthe area (including vacant buildings) Additionally, they were asked to rate the upkeep of nearby structures andthe presence of ... containers inthe streets; rating of most ofthe housing units inthe face block; and general rating ofthe condition ofthe street There are also logistical challenges in linking survey data from panel...
... translocation depending upon the biophysical properties of CPPs andtheir cargo [7,14,24] Interestingly, most investigations involving CPPs have been carried out in mammalian cell lines and there are only ... Chugh and F Eudes ing questions such as how the combined use of cell permeabilizing agent and cell-penetrating peptides results inthe delivery of such large sized cargoes in plant cells involving ... volume of 100 lL The contents ofthe two tubes were mixed together, giving a : peptide ⁄ protein ratio inthe mixture The mixture was incubated for h at room temperature and then added to the isolated...
... prove the global existence ofthe solutions of a delay differential equation, while inthe second example, we will obtain the bound ofthe solutions of an integral equation For the sake of proving ... general theorems and establish some new integral inequalities Also we will give some examples so as to illustrate the validity ofthe present integral inequalities Main results Inthe rest ofthe ... denote the set of real numbers as R, and R+ = [0, ∞) is a subset of R Dom(f) and Im(f) denote the definition domain andthe image of f, respectively Theorem 2.1: Assume that x, a Î C(R+, R+) and...
... Liscic B: State ofthe art in quenching In Quenching and Carburizing Proceedings ofthe Third International Seminar ofthe International Federation for Heat Treatment and Surface Engineering Edited ... whereas the measured hardness profile shows a continuous line inthe case of sample quenched inthe polymer solution having total wetting time of 1.5 s (Figure 5) [2] Thus, the type ofthe wetting ... nanoparticles increase the surface area andthe heat capacity ofthe fluid, (ii) the suspended nanoparticles increase the effective (or apparent) thermal conductivity ofthe fluid, (iii) the interaction and...
... further investigated by the TEM image as shown in Fig 1b, andthe intensive contrast between center and edge ofthe spheres indicates the formation of hollow structure inthe final products, and ... defined as the slope ofthe Ra/Rg versus c curve, and herein, Ra is the resistance value ofthe sensor in clean air, Rg is the resistance value ofthe sensor in specific gas under test and c is the HCHO ... adsorption–desorption isotherms and corresponding pore size distribution ofthe TiO2 product The isotherm shown inthe Fig 1d can be well classified as type IV isotherm, indicating the formation of a typical...
... easily inside the film, increasing the interfacial contact between the nanotubes, the dye, andthe electrolyte In addition, a high level of dye adsorption on TiO2 inthe form of nanorods and nanotubes ... thickness of 12 to 15 µm was deposited onto the pretreated conducting glass using the screen printing technique and sintered again at 450°C for 15 and at 500°C for 15 in air Assembly ofthe DSSCs The ... adsorb the sensitized dye, and enhance incident light harvest, the use of TNTs in DSSCs has been explored [14-15] Inthe present work, the effect of combining TiO2 nanoparticles with TNTs and the...
... and K?) on the surface of MnO2 and also possible intercalation/deintercalation of H? and alkaline metal cations inthe bulk of MnO2 [29] Since the 3-D dandelion-like microspheres ofthe sample ... 1036 substrate inthe thin film form The effects ofthe synthesis temperature on the morphology ofthe films are investigated, andthe capacitive behaviors of nanostructured MnO2 thin films with different ... of existing MnO2 sites, leading to the formation of 1-D nanowhiskers from the initial colloidal microspheres With increase inthe processing duration, finally dandelion-like 3-D microspheres of...
... 3.1 In this section, we obtain the application inthe algebraic Riccati equation of our results including 3.1 Some of our results and 3.1 cannot contain each other Theorem 3.1 If 1/p and K is the ... Majorization and Its Applications, vol 143 of Mathematics in Science and Engineering, Academic Press, New York, NY, USA, 1979 17 C.-L Wang, “On development of inverses ofthe Cauchy and Holder inequalities,” ... 2 Journal of Inequalities and Applications where x0 ∈ Rn is the initial state ofthe systems 1.1 and 1.2 , K is the positive definite solution ofthe following algebraic Riccati equation...